Bacteriorhodopsin/amphipol complexes: structural and functional properties.

abstract：:Members of the spectrin superfamily of proteins contain different numbers of homologous repeats arranged in tandem. Each of these consists of a three-alpha-helix motif, comprising two similarly and one oppositely directed alpha-helical segment joined by nonhelical linkers of characteristic length. The right-handed alp...

journal_title：Biophysical journal

authors： Parry DA,Dixon TW,Cohen C

更新日期：1992-04-01 00:00:00

abstract：:We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong la...

journal_title：Biophysical journal

authors： Zharov VP,Mercer KE,Galitovskaya EN,Smeltzer MS

更新日期：2006-01-15 00:00:00

abstract：:The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-06-01 00:00:00

abstract：:Biochemical systems lose their homogeneity at a mesoscopic scale; physical parameters vary sharply over a scale of a few nanometers.In this manuscript, we demonstrate how proton diffusion studies can report the microscopic properties of inhomogeneous systems.The method used for this purpose was the laser induced proto...

journal_title：Biophysical journal

authors： Gutman M,Nachliel E,Kiryati S

更新日期：1992-07-01 00:00:00

abstract：:Protein/lipid coassembly is an understudied phenomenon that is important to the function of antimicrobial peptides as well as the pathological effects of amyloid. Here, we study the coassembly process of PAP248-286, a seminal peptide that displays both amyloid-forming and antimicrobial activity. PAP248-286 is a peptid...

journal_title：Biophysical journal

authors： Vane EW,He S,Maibaum L,Nath A

更新日期：2020-09-01 00:00:00

abstract：:The presence of tryptophan residues as intrinsic fluorophores in most proteins makes them an obvious choice for fluorescence spectroscopic analyses of such proteins. Membrane proteins have been reported to have a significantly higher tryptophan content than soluble proteins. The role of tryptophan residues in the stru...

journal_title：Biophysical journal

authors： Chattopadhyay A,Mukherjee S,Rukmini R,Rawat SS,Sudha S

更新日期：1997-08-01 00:00:00

abstract：:The diffusion of beta-enolase and creatine phosphokinase in muscle cells has been studied by modulated fringe pattern photobleaching. Beta-enolase is mobile in the sarcoplasm. At 20 degrees C, the diffusion coefficient is 13.5 +/- 2.5 microm2 s(-1) in the cytosol and 56 microm2 s(-1) in aqueous media. As in the case o...

journal_title：Biophysical journal

authors： Arrio-Dupont M,Foucault G,Vacher M,Douhou A,Cribier S

更新日期：1997-11-01 00:00:00

abstract：:A requirement for concentrated and chemically homogeneous pools of molecular building blocks would severely restrict plausible scenarios for the origin of life. In the case of membrane self-assembly, models of prebiotic lipid synthesis yield primarily short, single-chain amphiphiles that can form bilayer vesicles only...

journal_title：Biophysical journal

authors： Budin I,Prwyes N,Zhang N,Szostak JW

更新日期：2014-10-07 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is a sensitive and widely used technique for measuring diffusion. FCS data are conventionally modeled with a finite number of diffusing components and fit with a least-square fitting algorithm. This approach is inadequate for analyzing data obtained from highly heterogeneous...

journal_title：Biophysical journal

authors： Sengupta P,Garai K,Balaji J,Periasamy N,Maiti S

更新日期：2003-03-01 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:The role played by non-homogeneous interactions in stabilizing cooperative structural changes in proteins was investigated by exhaustive simulations of all compact conformations compatible with several well-defined globule-like shapes in three dimensions. Conformational free energies corresponding to the association o...

journal_title：Biophysical journal

authors： Bahar I,Jernigan RL

更新日期：1994-02-01 00:00:00

abstract：:Deuterium magnetic resonance is applied to the study of the liquid crystalline and gel phases, and of the phase transition, of a multilamellar dispersion of chain perdeuterated (d62)-dipalmitoyl phosphatidylcholine/H2O. Analysis of the deuterium spectra in terms of the moments of the spectra allows one to make quantit...

journal_title：Biophysical journal

authors： Davis JH

更新日期：1979-09-01 00:00:00

abstract：:A recent thermodynamic model describes a reversible reaction between cholesterol (C) and phospholipid (P) to form a condensed complex C(nq)P(np). Here q and p are relatively prime integers used to define the stoichiometric composition, and n is a measure of cooperativity. The present study applies this model to the sc...

journal_title：Biophysical journal

authors： Anderson TG,McConnell HM

更新日期：2001-11-01 00:00:00

abstract：:The mouse major urinary protein (MUP) has proved to be an intriguing test bed for detailed studies on protein-ligand recognition. NMR, calorimetric, and modeling investigations have revealed that the thermodynamics of ligand binding involve a complex interplay between competing enthalpic and entropic terms. We perform...

journal_title：Biophysical journal

authors： Roy J,Laughton CA

更新日期：2010-07-07 00:00:00

abstract：:Dipole moment, enthalpy, and entropy changes were calculated for hypothetical structural units which control the opening and closing of ionic channels in axon membranes. The changes of these thermodynamic functions were calculated both for activation (transition to intermediate complex) and for the structural transfor...

journal_title：Biophysical journal

authors： Levitan E,Palti Y

更新日期：1975-03-01 00:00:00

abstract：:The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal...

journal_title：Biophysical journal

authors： Bullen A,Patel SS,Saggau P

更新日期：1997-07-01 00:00:00

abstract：:In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell deformation, which is comparable to the deformation in small capillaries. The shape recovery is recorded, and a relaxation time is obtained for each individual red...

journal_title：Biophysical journal

authors： Bronkhorst PJ,Streekstra GJ,Grimbergen J,Nijhof EJ,Sixma JJ,Brakenhoff GJ

更新日期：1995-11-01 00:00:00

abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...

journal_title：Biophysical journal

authors： Abbandonato G,Storti B,Tonazzini I,Stöckl M,Subramaniam V,Montis C,Nifosì R,Cecchini M,Signore G,Bizzarri R

更新日期：2019-02-05 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) is a powerful technique to study molecular dynamics inside living cells. During the past years, several laboratories have used FRAP to image the motion of RNA-protein and other macromolecular complexes in the nucleus and cytoplasm. In the case of mRNAs, there is growin...

journal_title：Biophysical journal

authors： Braga J,McNally JG,Carmo-Fonseca M

更新日期：2007-04-15 00:00:00

abstract：:The average sizes of fluid and gel domains in the two-component, two-phase system formed from mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine were determined from an analysis of the electron spin resonance spectral lineshapes of a dimyristoyl phosphatidylcholine-nitroxide spin label as a...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Thompson TE

更新日期：1992-08-01 00:00:00

abstract：:Fusion is a crucial event in the infection of animal cells by enveloped viruses (e.g., HIV or influenza). Viral fusion is mediated by glycoproteins, spanning the viral envelope, which attach to a membrane surface and induce fusion of the viral envelope to the cellular membrane. Influenza fusion protein (hemagglutinin)...

journal_title：Biophysical journal

authors： Longo ML,Waring AJ,Hammer DA

更新日期：1997-09-01 00:00:00

abstract：:The initial output of a time-resolved macromolecular crystallography experiment is a time-dependent series of difference electron density maps that displays the time-dependent changes in underlying structure as a reaction progresses. The goal is to interpret such data in terms of a small number of crystallographically...

journal_title：Biophysical journal

authors： Kostov KS,Moffat K

更新日期：2011-01-19 00:00:00

abstract：:We describe two enhancements of the planar bilayer recording method which enable low-noise recordings of single-channel currents activated by voltage steps in planar bilayers formed on apertures in partitions separating two open chambers. First, we have refined a simple and effective procedure for making small bilayer...

journal_title：Biophysical journal

authors： Wonderlin WF,Finkel A,French RJ

更新日期：1990-08-01 00:00:00

abstract：:Inactivation is a fundamental property of voltage-gated ion channels. Fast inactivation of Na(+) channels involves channel block by the III-IV cytoplasmic interdomain linker. The mechanisms of nonfast types of inactivation (intermediate, slow, and ultraslow) are unclear, although the ionic environment and P-loops rear...

journal_title：Biophysical journal

authors： Tikhonov DB,Zhorov BS

更新日期：2007-09-01 00:00:00

abstract：:Freeze-fracture electron micrographs from degranulating cells show that the lumen of the secretory granule is connected to the extracellular compartment via large (20 to 150 nm diameter) aqueous pores. These exocytotic fusion pores appear to be made up of a highly curved bilayer that spans the plasma and granule membr...

journal_title：Biophysical journal

authors： Nanavati C,Markin VS,Oberhauser AF,Fernandez JM

更新日期：1992-10-01 00:00:00

abstract：:Microbes inhabiting Earth have adapted to diverse environments of water, air, soil, and often at the interfaces of multiple media. In this study, we focus on the behavior of Caulobacter crescentus, a singly flagellated bacterium, at the air/water interface. Forward swimming C. crescentus swarmer cells tend to get phys...

journal_title：Biophysical journal

authors： Morse M,Huang A,Li G,Maxey MR,Tang JX

更新日期：2013-07-02 00:00:00

abstract：:Raman spectroscopic data indicate that the conformations of the two hydrocarbon chains of dipalmitoyl phosphatidylcholine in aqueous dispersions of the lipid differ signficantly. The compounds 1-palmitoyl, 2-palmitoyl-d31-3-sn-phosphatidylcholine and 1-palmitoyl-d31, 2-palmitoyl-3-sn-phosphatidylcholine were synthesiz...

journal_title：Biophysical journal

authors： Gaber BP,Yager P,Peticolas WL

更新日期：1978-12-01 00:00:00

abstract：:Mathematical models are increasingly important in biology, and testability is becoming a critical issue. One limitation is that one model simulation tests a parameter set representing one instance of the biological counterpart, whereas biological systems are heterogeneous in their properties and behavior, and a model ...

journal_title：Biophysical journal

authors： Tomaiuolo M,Bertram R,Leng G,Tabak J

更新日期：2012-11-07 00:00:00

abstract：:A mean-field statistical mechanical theory has been developed to describe molecular distributions in interphases. The excluded volume interaction has been modeled in terms of a reversible work that is required to create a cavity of the solute size against a pressure tensor exerted by the surrounding interphase molecul...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1994-03-01 00:00:00

abstract：:Some plant leaves display complex, orientation-dependent, proton nuclear magnetic resonance (1H NMR) spectra. The spectral patterns vary as the angle between the leaf surface and the applied magnetic field is varied. They also vary with temperature and with the quantity of absorbed manganous ions, but they are indepen...

journal_title：Biophysical journal

authors： McCain DC,Markley JL

更新日期：1985-11-01 00:00:00