Models of electrical activity: calibration and prediction testing on the same cell.

abstract：:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily...

journal_title：Biophysical journal

authors： Casuso I,Sens P,Rico F,Scheuring S

更新日期：2010-10-06 00:00:00

abstract：:A newly developed water-soluble phosphor suitable for measuring oxygen pressure in the blood (Green 2W) was used for noninvasive, in vivo imaging of oxygen distribution in the vascular systems of mice. Oxygen quenches the phosphorescence of Green 2W, measured in the presence of 2% albumin, according to the Stern-volme...

journal_title：Biophysical journal

authors： Vinogradov SA,Lo LW,Jenkins WT,Evans SM,Koch C,Wilson DF

更新日期：1996-04-01 00:00:00

abstract：:The activator protein-1 transcription factor is a heterodimer containing one of each of the Fos and Jun subfamilies of basic-region leucine-zipper proteins. We have previously shown by fluorescence cross-correlation spectroscopy (FCCS) that the fluorescent fusion proteins Fos-EGFP and Jun-mRFP1, cotransfected in HeLa ...

journal_title：Biophysical journal

authors： Vámosi G,Baudendistel N,von der Lieth CW,Szalóki N,Mocsár G,Müller G,Brázda P,Waldeck W,Damjanovich S,Langowski J,Tóth K

更新日期：2008-04-01 00:00:00

abstract：:Limited proteolysis of RNase-A yields a short N-terminal S-peptide segment and the larger S-protein. Binding of S-peptide to S-protein results in the formation of an enzymatically active RNase-S protein. S-peptide undergoes a transition from intrinsic disorder to an ordered helical state upon association with S-protei...

journal_title：Biophysical journal

authors： Luitz MP,Bomblies R,Zacharias M

更新日期：2017-10-03 00:00:00

abstract：:The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC). The thermodynamic parameters derived from all methods are remarkably similar and strongly suggest the high stabil...

journal_title：Biophysical journal

authors： Teles RC,Calderon Lde A,Medrano FJ,Barbosa JA,Guimarães BG,Santoro MM,de Freitas SM

更新日期：2005-05-01 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are attributed to envir...

journal_title：Biophysical journal

authors： Tata DB,Foresti M,Cordero J,Tomashefsky P,Alfano MA,Alfano RR

更新日期：1986-09-01 00:00:00

abstract：:Myosin subfragment 1 (S1) can be specifically modified at Lys-553 with the fluorescent probe FHS (6-[fluorescein-5(and 6)-carboxamido]hexanoic acid succinimidyl ester) (Bertrand, R., J. Derancourt, and R. Kassab. 1995. Biochemistry. 34:9500-9507), and solvent quenching of FHS-S1 with iodide has been shown to be sensit...

journal_title：Biophysical journal

authors： Cooper WC,Chrin LR,Berger CL

更新日期：2000-03-01 00:00:00

abstract：:Allosteric regulation of protein function is recognized to be widespread throughout biology; however, knowledge of allosteric mechanisms, the molecular changes within a protein that couple one binding site to another, is limited. Although mutagenesis is often used to probe allosteric mechanisms, we consider herein wha...

journal_title：Biophysical journal

authors： Carlson GM,Fenton AW

更新日期：2016-05-10 00:00:00

abstract：:We have obtained new insights into the behavior of a class of excitable systems when a stimulus, or parameter, is slowly tuned through a threshold value. Such systems do not accommodate no matter how slowly a stimulus ramp is applied, and the stimulus value at onset of repetitive activity shows a curious, nonmonotonic...

journal_title：Biophysical journal

authors： Rinzel J,Baer SM

更新日期：1988-09-01 00:00:00

abstract：:Back-of-the-envelope or rule-of-thumb calculations involving rough estimates of quantities play a central scientific role in developing intuition about the structure and behavior of physical systems, for example in so-called Fermi problems in the physical sciences. Such calculations can be used to powerfully and quant...

journal_title：Biophysical journal

authors： Johnston IG,Rickett BC,Jones NS

更新日期：2014-12-02 00:00:00

abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

journal_title：Biophysical journal

authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

更新日期：2010-07-21 00:00:00

abstract：:The thermophilic Bacillus caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-CspB) in 11 of the 66 residues. Stability measurements of Schmid and co-workers have implicated contributions of electrostatic interactions to the thermostability. To further elucid...

journal_title：Biophysical journal

authors： Zhou HX,Dong F

更新日期：2003-04-01 00:00:00

abstract：:Small RNA-mRNA binding is an essential step in RNA interference, an important cellular regulatory process. Calculations of binding free energy have been used in binding site prediction, but the cost of stretching the mRNA loop when the small RNA-mRNA duplex forms requires further exploration. Here, using both polymer ...

journal_title：Biophysical journal

authors： Meng Y,Aalberts DP

更新日期：2013-01-22 00:00:00

abstract：:Adenylate kinase, an enzyme that catalyzes the phosphoryl transfer between ATP and AMP, can interconvert between the open and catalytically potent (closed) forms even without binding ligands. Several aspects of the enzyme elasticity and internal dynamics are analyzed here by atomistic molecular dynamics simulations co...

journal_title：Biophysical journal

authors： Pontiggia F,Zen A,Micheletti C

更新日期：2008-12-15 00:00:00

abstract：:The effects of Mg++ on the spatial organization of nuclei from rat hepatocytes are analyzed in the range 0-60 mM, in the presence of suitable concentrations of KCl to reproduce physiological conditions. It is shown that the scatter-signal distribution measured by means of a flow microfluorimeter is greatly affected by...

journal_title：Biophysical journal

authors： Grattarola M,Carlo P,Giannetti G,Finollo R,Viviani R,Chiabrera A

更新日期：1985-04-01 00:00:00

abstract：:Universal features of the peptide aggregation process suggest a common mechanism, with a first-order phase transition in aqueous solutions of the peptides being the driving force. Small system sizes strongly affect the stability of the minor phase in the two-phase region. We show manifestations of this effect in aqueo...

journal_title：Biophysical journal

authors： Singh G,Brovchenko I,Oleinikova A,Winter R

更新日期：2008-10-01 00:00:00

abstract：:Nucleoprotein filament formation by recombinases is central to homologous recombination. To follow this process, we used fluorescent human Rad51 recombinase to visualize the interactions with double-stranded DNA (dsDNA). Fluorescence imaging revealed that Rad51 filament formation on dsDNA initiates from multiple nucle...

journal_title：Biophysical journal

authors： Mameren Jv,Modesti M,Kanaar R,Wyman C,Wuite GJ,Peterman EJ

更新日期：2006-10-15 00:00:00

abstract：:Many proteins are anchored to lipid bilayer membranes through a combination of hydrophobic and electrostatic interactions. In the case of the membrane-bound nonreceptor tyrosine kinase Src from Rous sarcoma virus, these interactions are mediated by an N-terminal myristoyl chain and an adjacent cluster of six basic ami...

journal_title：Biophysical journal

authors： Scheidt HA,Klingler J,Huster D,Keller S

更新日期：2015-08-04 00:00:00

abstract：:Fundamental mechanisms governing cell size control and homeostasis are still poorly understood. The relationship between sizes at division and birth in single cells is used as a metric to categorize the basis of size homeostasis. Cells dividing at a fixed size regardless of birth size (sizer) are expected to show a di...

journal_title：Biophysical journal

authors： Facchetti G,Knapp B,Chang F,Howard M

更新日期：2019-11-05 00:00:00

abstract：:The lateral mobility of the lipid analog N-4-nitrobenzo-2-oxa-1,3 diazole phosphatidylethanolamine and of the integral protein glycophorin in giant dimyristoylphosphatidylcholine vesicles was studied by the photobleaching technique. Above the temperature of the chain-melting transition (Tm = 23 degrees C), the diffusi...

journal_title：Biophysical journal

authors： Kapitza HG,Rüppel DA,Galla HJ,Sackmann E

更新日期：1984-03-01 00:00:00

abstract：:Mechanosensitive ion channels (MSCs) which could provide for fast osmoregulatory responses in bacteria, remain unidentified as molecular entities. MSCs from Escherichia coli (strain AW740) were examined using the patch-clamp technique, either (a) in giant spheroplasts, (b) after reconstitution by fusing native membran...

journal_title：Biophysical journal

authors： Sukharev SI,Martinac B,Arshavsky VY,Kung C

更新日期：1993-07-01 00:00:00

abstract：:Kv1 potassium channels are widely distributed in mammalian tissues and are involved in a variety of functions from controlling the firing rate of neurons to maturation of T-lymphocytes. Here we show that the newly described KCNE4 beta-subunit has a drastic inhibitory effect on currents generated by Kv1.1 and Kv1.3 pot...

journal_title：Biophysical journal

authors： Grunnet M,Rasmussen HB,Hay-Schmidt A,Rosenstierne M,Klaerke DA,Olesen SP,Jespersen T

更新日期：2003-09-01 00:00:00

abstract：:Secondary structure formation of nucleic acids strongly depends on salt concentration and temperature. We develop a theory for RNA folding that correctly accounts for sequence effects, the entropic contributions associated with loop formation, and salt effects. Using an iterative expression for the partition function ...

journal_title：Biophysical journal

authors： Einert TR,Netz RR

更新日期：2011-06-08 00:00:00

abstract：:Open channel properties of canine cardiac Purkinje cell Na+ channels were studied with single channel cell-attached recording and with whole cell macroscopic current recording in internally perfused cells. Single channel currents and membrane currents increased with an increase in Na+ concentration, but showed evidenc...

journal_title：Biophysical journal

authors： Sheets MF,Scanley BE,Hanck DA,Makielski JC,Fozzard HA

更新日期：1987-07-01 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:A lattice relaxation algorithm is developed to solve the Poisson-Nernst-Planck (PNP) equations for ion transport through arbitrary three-dimensional volumes. Calculations of systems characterized by simple parallel plate and cylindrical pore geometries are presented in order to calibrate the accuracy of the method. A ...

journal_title：Biophysical journal

authors： Kurnikova MG,Coalson RD,Graf P,Nitzan A

更新日期：1999-02-01 00:00:00

abstract：:Current methods for fluorescence resonance energy transfer (FRET) microscopy of living cells involve taking a series of images with alternating excitation colors in separate camera exposures. Here we present a new FRET method based on polarization that requires only one camera exposure and thereby offers the possibili...

journal_title：Biophysical journal

authors： Mattheyses AL,Hoppe AD,Axelrod D

更新日期：2004-10-01 00:00:00

abstract：:The interaction with lipids of a synthetic peptide corresponding to the transmembrane domain of influenza hemagglutinin was investigated by means of electron spin resonance. A detailed analysis of the electron spin resonance spectra from spin-labeled phospholipids revealed that the major effect of the peptide on the d...

journal_title：Biophysical journal

authors： Ge M,Freed JH

更新日期：2011-01-05 00:00:00

abstract：:Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditio...

journal_title：Biophysical journal

authors： Bédard C,Destexhe A

更新日期：2008-02-15 00:00:00