Polarized fluorescence resonance energy transfer microscopy.

abstract：:A long-standing question about membrane structure and function is the degree to which the physical properties of the inner and outer leaflets of a bilayer are coupled to one another. Using our recently developed methods to prepare asymmetric vesicles, coupling was investigated for vesicles containing phosphatidylcholi...

journal_title：Biophysical journal

authors： Chiantia S,London E

更新日期：2012-12-05 00:00:00

abstract：:Cell motility is essential in a variety of biological phenomena ranging from early development to organ homeostasis and diseases. This phenomenon has mainly been studied and characterized on flat surfaces in vitro, whereas such conditions are rarely observed in vivo. Recently, cell motion in three-dimensional microfab...

journal_title：Biophysical journal

authors： Le Maout E,Lo Vecchio S,Kumar Korla P,Jinn-Chyuan Sheu J,Riveline D

更新日期：2020-10-06 00:00:00

abstract：:Adsorption-desorption isotherms were obtained for water binding by 1,2-dimyristoylphosphatidylcholine in the temperature range 15 degrees-35 degrees C. The isotherms were analyzed by Brunauer et al.'s (BET) theory and also a polarization theory, the latter being more successful in fitting the data. There was some evid...

journal_title：Biophysical journal

authors： Wilkinson DA,Morowitz HJ,Prestegard JH

更新日期：1977-11-01 00:00:00

abstract：:The role of desolvation in protein binding kinetics is investigated using Brownian dynamics simulations in complexes in which the electrostatic interactions are relatively weak. We find that partial desolvation, modeled by a short-range atomic contact potential, is not only a major contributor to the binding free ener...

journal_title：Biophysical journal

authors： Camacho CJ,Kimura SR,DeLisi C,Vajda S

更新日期：2000-03-01 00:00:00

abstract：:We find evidence for a direct transition of insulin monomers into amyloid fibrils without measurable concentrations of oligomers or protofibrils, suggesting that fibrillogenesis may occur directly from assembly of denaturing insulin monomers rather than by successive transitions through protofibril nuclei. To support ...

journal_title：Biophysical journal

authors： Pease LF 3rd,Sorci M,Guha S,Tsai DH,Zachariah MR,Tarlov MJ,Belfort G

更新日期：2010-12-15 00:00:00

abstract：:It is shown that if x(t) is the solution of a second order differential equation, with real negative characteristic roots (not necessarily distinct), which exhibits an extremum at t = T, then T|x(T)|/|A| [UNK] 1/e where A is the area under the x(t) curve. This result is compared to a special case previously derived by...

journal_title：Biophysical journal

authors： HEARON JZ

更新日期：1961-09-01 00:00:00

abstract：:The force-extension curve of single myosin subfragment-1 molecules, interacting in the rigor state with an actin filament, has been investigated at low [ATP] by applying a slow triangle-wave movement to the optical traps holding a bead-actin-bead dumbbell. In combination with a measurement of the overall stiffness of ...

journal_title：Biophysical journal

authors： Lewalle A,Steffen W,Stevenson O,Ouyang Z,Sleep J

更新日期：2008-03-15 00:00:00

abstract：:The outer membrane porin OmpF from Escherichia coli has been reconstituted into lipid bilayers of defined composition, and fluorescence spectroscopy is used to characterize its interaction with the surrounding lipid. OmpF is a trimer within the membrane. It contains two Trp residues per monomer, Trp(214) at the lipid-...

journal_title：Biophysical journal

authors： O'Keeffe AH,East JM,Lee AG

更新日期：2000-10-01 00:00:00

abstract：:Protein solubility in aqueous solutions depends in a complicated and not well understood way on pH, salt type, and salt concentration. Why for instance does the use of two different monovalent salts, potassium thiocyanate and potassium chloride, produce such different results? One important and previously neglected so...

journal_title：Biophysical journal

authors： Boström M,Williams DR,Ninham BW

更新日期：2003-08-01 00:00:00

abstract：:We have measured the electronic circular dichroism (ECD) of the ferri- and ferro-states of several natural cytochrome c derivatives (horse heart, chicken, bovine, and yeast) and the Y67F mutant of yeast in the region between 300 and 750 nm. Thus, we recorded the ECD of the B- and Q-band region as well as the charge-tr...

journal_title：Biophysical journal

authors： Dragomir I,Hagarman A,Wallace C,Schweitzer-Stenner R

更新日期：2007-02-01 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Recently, several studies have shown that short doubled-stranded DNA (dsDNA) loops more readily than the wormlike chain model predicts. In most of these experiments, the intrinsic bendedness of dsDNA, which in theory can dramatically influence looping dynamics, was either avoided or unaccounted for. To investigate the...

journal_title：Biophysical journal

authors： Le TT,Kim HD

更新日期：2013-05-07 00:00:00

abstract：:Rapid and robust methods are required to quantify the effect of hydrodynamic shear on protein conformation change. We evaluated such strategies in this work and found that the binding of the fluorescent probe 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid (bis-ANS) to hydrophobic pockets in the blood protein von ...

journal_title：Biophysical journal

authors： Themistou E,Singh I,Shang C,Balu-Iyer SV,Alexandridis P,Neelamegham S

更新日期：2009-11-04 00:00:00

abstract：:Cryoelectron microscopy has been used to study the reorganization of unilamellar cationic lipid vesicles upon the addition of DNA. Unilamellar DNA-coated vesicles, as well as multilamellar DNA lipid complexes, could be observed. Also, DNA induced fusion of unilamellar vesicles was found. DNA appears to adsorb to the o...

journal_title：Biophysical journal

authors： Huebner S,Battersby BJ,Grimm R,Cevc G

更新日期：1999-06-01 00:00:00

abstract：:The binding mechanism of sweet proteins to their receptor, a G-protein-coupled receptor, is not supported by direct structural information. In principle, the key groups responsible for biological activity (glucophores) can be localized on a small structural unit (sweet finger) or spread on a larger surface area. A rec...

journal_title：Biophysical journal

authors： De Simone A,Spadaccini R,Temussi PA,Fraternali F

更新日期：2006-05-01 00:00:00

abstract：:M2delta, one of the transmembrane segments of the nicotinic acetylcholine receptor, is a 23-amino-acid peptide, frequently used as a model for peptide-membrane interactions. In this and the companion article we describe studies of M2delta-membrane interactions, using two different computational approaches. In the pres...

journal_title：Biophysical journal

authors： Kessel A,Haliloglu T,Ben-Tal N

更新日期：2003-12-01 00:00:00

abstract：:The photophysics of the complex forming reaction of Ca2+ and Fura-2 are investigated using steady-state and time-resolved fluorescence measurements. The fluorescence decay traces were analyzed with global compartmental analysis yielding the following values for the rate constants at room temperature in aqueous solutio...

journal_title：Biophysical journal

authors： Van den Bergh V,Boens N,De Schryver FC,Ameloot M,Steels P,Gallay J,Vincent M,Kowalczyk A

更新日期：1995-03-01 00:00:00

abstract：:We performed hole-burning Stark effect experiments on cytochrome c in which the iron of the herne was either removed or replaced by Zn. According to the experiments, the free-base compound has an effective inversion center, even in the protein. The Zn compound, on the other hand, shows quite peculiar features: in the ...

journal_title：Biophysical journal

authors： Köhler M,Gafert J,Friedrich J,Vanderkooi JM,Laberge M

更新日期：1996-07-01 00:00:00

abstract：:Clusters of calcium-loaded sarcoplasmic reticulum (SR) vesicles in agarose gel were previously shown to behave as an excitable medium that propagates calcium waves. In a 3D-hexagonal disposition, the distance between neighboring spheres (which may stand for SR vesicles) is constant and the relationship between distanc...

journal_title：Biophysical journal

authors： Wussling MH,Aurich I,Knauf O,Podhaisky H,Holzhausen HJ

更新日期：2004-12-01 00:00:00

abstract：:Molecular dynamics simulations of the tripeptide Ala-Phe-Ala-O-tert-butyl interacting with dimyristoylphosphatidylcholine lipid bilayers have been carried out. The lipid and aqueous environments of the peptide, the alkyl chain order, and the lipid and peptide dynamics have been investigated with use of density profile...

journal_title：Biophysical journal

authors： Damodaran KV,Merz KM Jr,Gaber BP

更新日期：1995-10-01 00:00:00

abstract：:We present elastic and inelastic incoherent neutron scattering data from a series of trehalose glasses diluted with glycerol. A strong correlation with recently published protein stability data in the same series of glasses illustrates that the dynamics at Q >or= 0.71 A(-1) and omega > 200 MHz are important to stabili...

journal_title：Biophysical journal

authors： Cicerone MT,Soles CL

更新日期：2004-06-01 00:00:00

abstract：:The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal...

journal_title：Biophysical journal

authors： Charras GT,Horton MA

更新日期：2002-06-01 00:00:00

abstract：:The AXH domain of protein Ataxin 1 is thought to play a key role in the misfolding and aggregation pathway responsible for Spinocerebellar ataxia 1. For this reason, a molecular level understanding of AXH oligomerization pathway is crucial to elucidate the aggregation mechanism, which is thought to trigger the disease...

journal_title：Biophysical journal

authors： Grasso G,Morbiducci U,Massai D,Tuszynski JA,Danani A,Deriu MA

更新日期：2018-01-23 00:00:00

abstract：:Solid phase amplification (SPA), a new method to amplify DNA, is characterized by the use of surface-bound primers. This limits the amplification to two-dimensional surfaces and therefore allows the easy parallelization of DNA amplification in a single system. SPA leads to the formation of small but dense DNA brushes,...

journal_title：Biophysical journal

authors： Mercier JF,Slater GW

更新日期：2005-07-01 00:00:00

abstract：:We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). This compound is unique since both its acid and base forms possess different fluorescence emiss...

journal_title：Biophysical journal

authors： Kurtz I,Balaban RS

更新日期：1985-09-01 00:00:00

abstract：:We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These chang...

journal_title：Biophysical journal

authors： Miake-Lye RC,Doniach S,Hodgson KO

更新日期：1983-03-01 00:00:00

abstract：:Accommodation may be defined as an increase in the threshold of an excitable membrane when the membrane is subjected to a sustained subthreshold depolarizing stimulus. Some excitable membranes show accommodation in response to currents which rise linearly at a very slow rate. In this report we point out a theoretical ...

journal_title：Biophysical journal

authors： Jakobsson E,Guttman R

更新日期：1980-08-01 00:00:00

abstract：:The size of a cell is central to many functions, including cellular communication and exchange of materials with the environment. This modeling and experimental study focused on understanding how the size of a cell determines its ability to uptake nanometer-scale extracellular materials from the environment. Several m...

journal_title：Biophysical journal

authors： Khetan J,Shahinuzzaman M,Barua S,Barua D

更新日期：2019-01-22 00:00:00

abstract：:Both the solution and the oriented film absorption and circular dichroic spectra of the bacteriorhodopsin (bR(568)) and M(412) intermediate of the purple membrane photocycle were compared over the wavelength region 800-183 nm to assess structural changes during this photocycle. The main findings are (a) loss of the ex...

journal_title：Biophysical journal

authors： Draheim JE,Cassim JY

更新日期：1985-04-01 00:00:00

abstract：:Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. Application of the dual whole-cell voltage clamp technique with patch electrodes to pairs of transfected SKHep1 cells revealed strong sensit...

journal_title：Biophysical journal

authors： Moreno AP,Eghbali B,Spray DC

更新日期：1991-11-01 00:00:00