Selectivity in lipid binding to the bacterial outer membrane protein OmpF.

abstract：:Myelin basic protein (MBP) is predominantly found in the membranes of the myelin sheath of the central nervous system and is involved in important protein-protein and protein-lipid interactions in vivo and in vitro. Furthermore, divalent transition metal ions, especially Zn(2+) and Cu(2+), seem to directly affect the ...

journal_title：Biophysical journal

authors： Bund T,Boggs JM,Harauz G,Hellmann N,Hinderberger D

更新日期：2010-11-03 00:00:00

abstract：:The results of a 3-ns molecular dynamics simulation of the dodecamer duplex d(TATGGATCCATA)(2) recognized by the BamHI endonuclease are presented here. The DNA has been simulated as a flexible molecule using an AMBER force field and the Ewald summation method, which eliminates the undesired effects of truncation and p...

journal_title：Biophysical journal

authors： Castrignanò T,Chillemi G,Desideri A

更新日期：2000-09-01 00:00:00

abstract：:The time dependence of magnetic field effects on light absorption by triplet-state and radical ions in quinone-depleted reaction centers of Rhodopseudomonas sphaeroides strain R-26 has been investigated. Measurements on the time scale of the hyperfine interaction in the radical pair [(BChl)2+. ...BPh-.)] provided kine...

journal_title：Biophysical journal

authors： Ogrodnik A,Krüger HW,Orthuber H,Haberkorn R,Michel-Beyerle ME,Scheer H

更新日期：1982-07-01 00:00:00

abstract：:The temperature and voltage dependence of gating and conductance of sarcoplasmic reticulum K+ channels (S-R K+) isolated from adult canine hearts were studied using the reconstituted bilayer technique. Fusion of vesicles from this preparation frequently resulted in the incorporation of a single channel. Only bilayers ...

journal_title：Biophysical journal

authors： Shen WK,Rasmusson RL,Liu QY,Crews AL,Strauss HC

更新日期：1993-08-01 00:00:00

abstract：:The kinetics of redistribution of lipid-like molecules between the membranes of two fused spherical vesicles is studied by solving the time-dependent diffusion equation of the system. The effects on the probe redistribution rate of pore size at the fusion junction and the relative sizes of the vesicles are examined. I...

journal_title：Biophysical journal

authors： Rubin RJ,Chen YD

更新日期：1990-11-01 00:00:00

abstract：:In biology, molecular linkages at, within, and beneath cell interfaces arise mainly from weak noncovalent interactions. These bonds will fail under any level of pulling force if held for sufficient time. Thus, when tested with ultrasensitive force probes, we expect cohesive material strength and strength of adhesion a...

journal_title：Biophysical journal

authors： Evans E,Ritchie K

更新日期：1997-04-01 00:00:00

abstract：:We have used the elastic neutron scattering technique to investigate the dynamics of the two main saccharidic components of starch: amylose and amylopectin. The measurements were carried out in the temperature range of 20 to 320 K and at different hydration levels from the dry state up to 0.47 g saccharide/g D(2)O. In...

journal_title：Biophysical journal

authors： Di Bari M,Cavatorta F,Deriu A,Albanese G

更新日期：2001-08-01 00:00:00

abstract：:Forces generated by microtubule polymerization and depolymerization are important for the biological functioning of cells. The mean growth velocity, V, under an opposing force, F, has been measured by; Science 278:856-860) for single microtubules growing in vitro, but their analysis of the data suggested that V decrea...

journal_title：Biophysical journal

authors： Kolomeisky AB,Fisher ME

更新日期：2001-01-01 00:00:00

abstract：:The yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, toposome, accounts for approximately 50% of the total yolk protein and has been shown to be localized to the embryonic cell surface. Extensive characterization in severa...

journal_title：Biophysical journal

authors： Hayley M,Emberley J,Davis PJ,Morrow MR,Robinson JJ

更新日期：2006-12-15 00:00:00

abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

journal_title：Biophysical journal

authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

更新日期：2010-07-21 00:00:00

abstract：:Cellular differentiation proceeds along complicated pathways, even when it is induced by extracellular signaling molecules. One of the major reasons for this complexity is the highly multidimensional internal dynamics of cells, which sometimes causes apparently stochastic responses in individual cells to extracellular...

journal_title：Biophysical journal

authors： Morita S,Takanezawa S,Hiroshima M,Mitsui T,Ozaki Y,Sako Y

更新日期：2014-11-18 00:00:00

abstract：:Nonphotochemical quenching is the protective mechanism against overexcitation of photosystem II, triggered by excess ΔpH in photosynthetic membranes. The light-harvesting complexes (LHCs), the de-epoxidation of violaxanthin to zeaxanthin, and the photosystem II subunit S (PsbS) work in synergy for an optimized multile...

journal_title：Biophysical journal

authors： Daskalakis V,Papadatos S

更新日期：2017-12-05 00:00:00

abstract：:A first principles model for calculating hydrogen bonding interactions, previously applied to water, is here applied to the more difficult problem of interactions between DNA bases. We first consider the energetics and geometry for the A-T and the G-C basepairs, comparing our results to other calculated results as wel...

journal_title：Biophysical journal

authors： Lewis JP,Sankey OF

更新日期：1995-09-01 00:00:00

abstract：:Intracellular transport of cargos along microtubules is often complicated by the topology of the underlying filament network. The fundamental building blocks for this complex arrangement are filament intersections. The navigation of cargos across microtubule intersections remains poorly understood. Here, we demonstrat...

journal_title：Biophysical journal

authors： Osunbayo O,Butterfield J,Bergman J,Mershon L,Rodionov V,Vershinin M

更新日期：2015-03-24 00:00:00

abstract：:The role of desolvation in protein binding kinetics is investigated using Brownian dynamics simulations in complexes in which the electrostatic interactions are relatively weak. We find that partial desolvation, modeled by a short-range atomic contact potential, is not only a major contributor to the binding free ener...

journal_title：Biophysical journal

authors： Camacho CJ,Kimura SR,DeLisi C,Vajda S

更新日期：2000-03-01 00:00:00

abstract：:We present a theoretical and experimental analysis of the effects of nonlinear flow in a cone-plate viscometer. The analysis predicts that flow in the viscometer is a function of two parameters, the Reynolds number and the cone angle. Nonlinear flow occurs at high shear rates and causes spatial variations in wall shea...

journal_title：Biophysical journal

authors： Shankaran H,Neelamegham S

更新日期：2001-06-01 00:00:00

abstract：:Human transthyretin (TTR) is an amyloidogenic protein. The pathway of TTR amyloid formation has been proposed based on lines of evidence: TTR tetramer first dissociates into native monomers, which is shown to be a rate-limiting step in the formation of fibrils. Subsequently, the monomeric species partially unfold to f...

journal_title：Biophysical journal

authors： Yang M,Lei M,Bruschweiler R,Huo S

更新日期：2005-07-01 00:00:00

abstract：:Certain standard properties, including spontaneous curvature, are assumed for the membrane of a bilipid vesicle. Then, if there is a mechanism that causes the membrane area to increase, vesicle cleavage is found to occur. ...

journal_title：Biophysical journal

authors： Luke JC,Kaplan JI

更新日期：1979-01-01 00:00:00

abstract：:We present here the parmbsc0 force field, a refinement of the AMBER parm99 force field, where emphasis has been made on the correct representation of the alpha/gamma concerted rotation in nucleic acids (NAs). The modified force field corrects overpopulations of the alpha/gamma = (g+,t) backbone that were seen in long ...

journal_title：Biophysical journal

authors： Pérez A,Marchán I,Svozil D,Sponer J,Cheatham TE 3rd,Laughton CA,Orozco M

更新日期：2007-06-01 00:00:00

abstract：:The observation of biological processes at the molecular scale in real time requires high spatial and temporal resolution. Magnetic tweezers are straightforward to implement, free of radiation or photodamage, and provide ample multiplexing capability, but their spatiotemporal resolution has lagged behind that of other...

journal_title：Biophysical journal

authors： Dulin D,Cui TJ,Cnossen J,Docter MW,Lipfert J,Dekker NH

更新日期：2015-11-17 00:00:00

abstract：:Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. ...

journal_title：Biophysical journal

authors： Alexandre MT,Domratcheva T,Bonetti C,van Wilderen LJ,van Grondelle R,Groot ML,Hellingwerf KJ,Kennis JT

更新日期：2009-07-08 00:00:00

abstract：:Many animals regulate power generation for locomotion by varying the number of muscle fibers used for movement. However, insects with asynchronous flight muscles may regulate the power required for flight by varying the calcium concentration ([Ca(2+)]). In vivo myoplasmic calcium levels in Drosophila flight muscle hav...

journal_title：Biophysical journal

authors： Wang Q,Zhao C,Swank DM

更新日期：2011-11-02 00:00:00

abstract：:We examined the state-, voltage-, and time dependences of interaction between 4-AP and a mammalian A-type K channel clone (rKv1.4) expressed in Xenopus oocytes using whole-cell and single-channel recordings. 4-AP blocked rKv1.4 from the cytoplasmic side of the membrane. The development of block required channel openin...

journal_title：Biophysical journal

authors： Yao JA,Tseng GN

更新日期：1994-07-01 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:A significant amount of work has been devoted to obtaining a detailed atomistic knowledge of the human hemoglobin mechanism. Despite this impressive research, to date, the ligand diffusion processes remain unclear and controversial. Using recently developed computational techniques, PELE, we are capable of addressing ...

journal_title：Biophysical journal

authors： Lucas MF,Guallar V

更新日期：2012-02-22 00:00:00

abstract：:N-methyl-d-aspartate (NMDA) receptors are glutamate- and glycine-gated channels that flux Na+ and Ca2+ into postsynaptic neurons during synaptic transmission. The resulting intracellular Ca2+ transient is essential to physiological and pathological processes related to synaptic development, plasticity, and apoptosis. ...

journal_title：Biophysical journal

authors： Iacobucci GJ,Popescu GK

更新日期：2017-11-21 00:00:00

abstract：:We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicl...

journal_title：Biophysical journal

authors： Shtrahman M,Yeung C,Nauen DW,Bi GQ,Wu XL

更新日期：2005-11-01 00:00:00

abstract：:Molecular dynamics simulations in simplified models allow one to study the scaling properties of folding times for many proteins together under a controlled setting. We consider three variants of the Go models with different contact potentials and demonstrate scaling described by power laws and no correlation with the...

journal_title：Biophysical journal

authors： Cieplak M,Hoang TX

更新日期：2003-01-01 00:00:00

abstract：:It is widely assumed that the decay of fluorescence in photosynthetic systems can be described as a sum of exponential components and that the amplitude of each component is directly related to the absorption cross-section of the antenna pigments coupled to the fluorescing species. We present exact calculations of exc...

journal_title：Biophysical journal

authors： Jean JM,Chan CK,Fleming GR,Owens TG

更新日期：1989-12-01 00:00:00

abstract：:A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found for the various parts of the life cycle: S, 4.13 hours; G1, 4.71 hours; G2, 2.81 hours; mitosis, 0.81 hours. The cell has a to...

journal_title：Biophysical journal

authors： PUCK TT,SANDERS P,PETERSEN D

更新日期：1964-11-01 00:00:00