Laser flow measurements of scattering and fluorescence from cell nuclei in the presence of increasing Mg++ concentrations.

abstract：:Recent biological optical spectroscopic studies have correlated discrete spectroscopic states with biological function in several systems. One of the challenges of molecular biophysics is to correlate structural changes with these spectroscopic states. From small-angle x-ray scattering one can obtain a key structural ...

journal_title：Biophysical journal

authors： Stamatoff J

更新日期：1979-05-01 00:00:00

abstract：:It is often assumed that pH(i) is spatially uniform within cells. A double-barreled microperfusion system was used to apply solutions of weak acid (acetic acid, CO(2)) or base (ammonia) to localized regions of an isolated ventricular myocyte (guinea pig). A stable, longitudinal pH(i) gradient (up to 1 pH(i) unit) was ...

journal_title：Biophysical journal

authors： Swietach P,Leem CH,Spitzer KW,Vaughan-Jones RD

更新日期：2005-04-01 00:00:00

abstract：:De novo formation of cell pairs was used to examine the gating properties of single gap junction channels. Two separate cells of an insect cell line (clone C6/36, derived from the mosquito Aedes albopictus) were pushed against each other to provoke formation of gap junction channels. A dual voltage-clamp method was us...

journal_title：Biophysical journal

authors： Bukauskas FF,Weingart R

更新日期：1994-08-01 00:00:00

abstract：:Many proteins "bind" DNA through positively charged amino acids on their surfaces. However, to overcome significant energetic and topological obstacles, proteins that bend or package DNA might also modulate the stiffness that is generated by repulsions between phosphates within DNA. Much previous work describes how io...

journal_title：Biophysical journal

authors： Podestà A,Indrieri M,Brogioli D,Manning GS,Milani P,Guerra R,Finzi L,Dunlap D

更新日期：2005-10-01 00:00:00

abstract：:We have used confocal microscopy to examine the [Ca2+]i increase in the albino eggs of the frog Xenopus laevis after fertilization. Eggs were placed in agar wells with their animal poles downward so that fertilization occurred preferentially in the equatorial plane, and confocal microscopy was used to provide a two-di...

journal_title：Biophysical journal

authors： Fontanilla RA,Nuccitelli R

更新日期：1998-10-01 00:00:00

abstract：:Conduction speed (theta) in single myelinated Rana pipiens sciatic nerve fibers has been precisely measured using intracellular recording and on-line digital computer techniques. The dependence of relative speed on external Na concentration at 15 degrees C has been found to be ln(theta(1)/theta(2)) = 0.524 (+/-0.018) ...

journal_title：Biophysical journal

authors： Hardy WL

更新日期：1973-10-01 00:00:00

abstract：:The binding mechanism of sweet proteins to their receptor, a G-protein-coupled receptor, is not supported by direct structural information. In principle, the key groups responsible for biological activity (glucophores) can be localized on a small structural unit (sweet finger) or spread on a larger surface area. A rec...

journal_title：Biophysical journal

authors： De Simone A,Spadaccini R,Temussi PA,Fraternali F

更新日期：2006-05-01 00:00:00

abstract：:The efficiency of various patterns of pulsatile stimulation is determined in a model in which a receptor becomes desensitized in the presence of its stimulatory ligand. The effect of stochastic or chaotic changes in the duration and/or interval between successive pulses in a series of square-wave stimuli is investigat...

journal_title：Biophysical journal

authors： Li Y,Goldbeter A

更新日期：1992-01-01 00:00:00

abstract：:Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

journal_title：Biophysical journal

authors： Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

更新日期：2008-06-01 00:00:00

abstract：:We show prolonged contraction of permeabilized muscle fibers of the frog during which structural order, as judged from low-angle x-ray diffraction, was preserved by means of partial cross-linking of the fibers using the zero-length cross-linker 1-ethyl-3-[3-dimethylamino)propyl]carbodiimide. Ten to twenty percent of t...

journal_title：Biophysical journal

authors： Bershitsky S,Tsaturyan A,Bershitskaya O,Mashanov G,Brown P,Webb M,Ferenczi MA

更新日期：1996-09-01 00:00:00

abstract：:The physical mechanism underlying the voltage-dependent gating of K channels is usually addressed theoretically using molecular dynamics simulations. However, besides being computationally very expensive, this approach is presently unable to fully predict the behavior of fundamental variables of channel gating such as...

journal_title：Biophysical journal

authors： Catacuzzeno L,Franciolini F

更新日期：2019-11-19 00:00:00

abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....

journal_title：Biophysical journal

authors： Wei P,Zheng BK,Guo PR,Kawakami T,Luo SZ

更新日期：2013-04-02 00:00:00

abstract：:Many experiments have measured the effect of force on the dissociation of single selectin bonds, but it is not yet clear how the force dependence of molecular dissociation can influence the rolling of cells expressing selectin molecules. Recent experiments using constant-force atomic force microscopy or high-resolutio...

journal_title：Biophysical journal

authors： Caputo KE,Lee D,King MR,Hammer DA

更新日期：2007-02-01 00:00:00

abstract：:Molecular chaperones are large proteins or protein complexes from which many proteins require assistance in order to fold. One unique property of molecular chaperones is the cavity they provide in which proteins fold. The interior surface residues which make up the cavities of molecular chaperone complexes from differ...

journal_title：Biophysical journal

authors： White AD,Huang W,Jiang S

更新日期：2012-12-19 00:00:00

abstract：:Fluorescence cross-correlation spectroscopy (FCCS) is used to determine interactions and dissociation constants (K(d)s) of biomolecules. The determination of a K(d) depends on the accurate measurement of the auto- and cross-correlation function (ACF and CCF) amplitudes. In the case of complete binding, the ratio of th...

journal_title：Biophysical journal

authors： Foo YH,Naredi-Rainer N,Lamb DC,Ahmed S,Wohland T

更新日期：2012-03-07 00:00:00

abstract：:Biological cells embedded in fibrous matrices have been observed to form intercellular bands of dense and aligned fibers through which they mechanically interact over long distances. Such matrix-mediated cellular interactions have been shown to regulate various biological processes. This study aimed to explore the eff...

journal_title：Biophysical journal

authors： Sopher RS,Tokash H,Natan S,Sharabi M,Shelah O,Tchaicheeyan O,Lesman A

更新日期：2018-10-02 00:00:00

abstract：:The addition of 10(-3) M p-chloromercuribenzene sulfonate (PCMBS) to the solution bathing the mucosal surface of rabbit colon has no effect on the rate of active Na transport but blocks or reverses the inhibitory action of amiloride. The tissue must be exposed to PCMBS for 20-30 min for a complete blocking effect, and...

journal_title：Biophysical journal

authors： Gottlieb GP,Turnheim K,Frizzell RA,Schultz SG

更新日期：1978-04-01 00:00:00

abstract：:The domains of the giant muscle protein titin (connectin) provide interaction sites for other sarcomeric proteins and fulfill mechanical functions. In this paper we compare the unfolding forces of defined regions of different titin isoforms by single-molecule force spectroscopy. Constructs comprising six to eight immu...

journal_title：Biophysical journal

authors： Rief M,Gautel M,Schemmel A,Gaub HE

更新日期：1998-12-01 00:00:00

abstract：:Molecular motors are thought to generate force and directional motion via nonequilibrium switching between energy surfaces. Because all enzymes can undergo such switching, we hypothesized that the ability to generate rotary motion and torque is not unique to highly adapted biological motor proteins but is instead a co...

journal_title：Biophysical journal

authors： Slochower DR,Gilson MK

更新日期：2018-05-08 00:00:00

abstract：:The two glycosphingolipids galactosylceramide (GalC) and its sulfated form, cerebroside sulfate (CBS), are present at high concentrations in the multilayered myelin sheath and are involved in carbohydrate-carbohydrate interactions between the lipid headgroups. In order to study the structure of the complex of these tw...

journal_title：Biophysical journal

authors： Boggs JM,Menikh A,Rangaraj G

更新日期：2000-02-01 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:We have developed a model for the analysis of the forward creatine kinase reaction in muscle as measured by the nuclear magnetic resonance (NMR) technique of magnetization transfer. The model, accounting for the double-exponential behavior observed in some NMR magnetization transfer data, allows for the existence of t...

journal_title：Biophysical journal

authors： Zahler R,Bittl JA,Ingwall JS

更新日期：1987-06-01 00:00:00

abstract：:The excess oxygen concentration in the photosynthetic membranes of functioning oxygenic photosynthetic cells was estimated using classical diffusion theory combined with experimental data on oxygen production rates of cyanobacterial cells. The excess oxygen concentration within the plesiomorphic cyanobacterium Gloeoba...

journal_title：Biophysical journal

authors： Kihara S,Hartzler DA,Savikhin S

更新日期：2014-05-06 00:00:00

abstract：:We have studied the low-resolution solution conformation of a Holliday (or four-way) DNA junction by using small-angle x-ray scattering, sedimentation velocity, and computational modeling techniques. The scattering data were analyzed in two independent ways: firstly, by rigid-body modeling of the scattering data using...

journal_title：Biophysical journal

authors： Nöllmann M,Stark WM,Byron O

更新日期：2004-05-01 00:00:00

abstract：:Molecular diffusion process after the photo-induced electron injection to ferric cytochrome c (Fe(III) cyt c) in guanidine hydrochloride (GdnHCl) 3.5 M buffer solution is studied by the time-resolved transient grating technique. Circular dichroism studies have revealed that Fe(III) cyt c is unfolded under this conditi...

journal_title：Biophysical journal

authors： Nada T,Terazima M

更新日期：2003-09-01 00:00:00

abstract：:This work presents a discrete multidomain model that describes ionic diffusion pathways between connected cells and within the interstitium. Unlike classical models of impulse propagation, the intracellular and extracellular spaces are represented as spatially distinct volumes with dynamic/static boundary conditions t...

journal_title：Biophysical journal

authors： Roberts SF,Stinstra JG,Henriquez CS

更新日期：2008-10-01 00:00:00

abstract：:High-bandwidth measurements of the ion current through hafnium oxide and silicon nitride nanopores allow the analysis of sub-30 kD protein molecules with unprecedented time resolution and detection efficiency. Measured capture rates suggest that at moderate transmembrane bias values, a substantial fraction of protein ...

journal_title：Biophysical journal

authors： Larkin J,Henley RY,Muthukumar M,Rosenstein JK,Wanunu M

更新日期：2014-02-04 00:00:00

abstract：:Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress condition...

journal_title：Biophysical journal

authors： Leekumjorn S,Wu Y,Sum AK,Chan C

更新日期：2008-04-01 00:00:00

abstract：:The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...

journal_title：Biophysical journal

authors： Lacroix JJ,Bezanilla F

更新日期：2012-08-08 00:00:00

abstract：:The prion protein (PrP), widely recognized to misfold into the causative agent of the transmissible spongiform encephalopathies, has previously been shown to bind to lipid membranes with binding influenced by both membrane composition and pH. Aside from the misfolding events associated with prion pathogenesis, PrP can...

journal_title：Biophysical journal

authors： Le Brun AP,Haigh CL,Drew SC,James M,Boland MP,Collins SJ

更新日期：2014-11-18 00:00:00