Abstract:
:We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong laser-induced overheating effects accompanied by the bubble-formation phenomena around clustered gold nanoparticles are the main cause of bacterial damage. PT imaging and time-resolved monitoring of the integrated PT responses assessed these effects. Specifically, we used this technology for selective killing of the Gram-positive bacterium Staphylococcus aureus by targeting the bacterial surface using 10-, 20-, and 40-nm gold particles conjugated with anti-protein A antibodies. Labeled bacteria were irradiated with focused laser pulses (420-570 nm, 12 ns, 0.1-5 J/cm(2), 100 pulses), and laser-induced bacterial damage observed at different laser fluences and nanoparticle sizes was verified by optical transmission, electron microscopy, and conventional viability testing.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Zharov VP,Mercer KE,Galitovskaya EN,Smeltzer MSdoi
10.1529/biophysj.105.061895subject
Has Abstractpub_date
2006-01-15 00:00:00pages
619-27issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(06)72240-8journal_volume
90pub_type
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