Birefringence Changes of Dendrites in Mouse Hippocampal Slices Revealed with Polarizing Microscopy.

abstract：:Dielectrophoresis and electrorotation are commonly used to measure dielectric properties and membrane electrical parameters of biological cells. We have derived quantitative relationships for several critical points, defined in Fig. A 1, which characterize the dielectrophoretic spectrum and the electrorotational spect...

journal_title：Biophysical journal

authors： Gimsa J,Marszalek P,Loewe U,Tsong TY

更新日期：1991-10-01 00:00:00

abstract：:The rate of muscle force redevelopment after release-restretch protocols has previously been interpreted using a simple two-state cross-bridge cycling model with rate constants for transitions between non-force-bearing and force-bearing states, f, and between force-bearing and non-force-bearing states, g. Changes in t...

journal_title：Biophysical journal

authors： Campbell K

更新日期：1997-01-01 00:00:00

abstract：:The electric quadrupole moment of the deuterium nucleus provides a nuclear magnetic resonance (NMR) probe of electric field gradients, and thereby of organization of tissue water. 8-17% of H(2)O in rat muscle and brain was replaced by D(2)O from 50% deuterated drinking water. The peak height of the steady-state NMR sp...

journal_title：Biophysical journal

authors： Cope FW

更新日期：1969-03-01 00:00:00

abstract：:High-affinity and cooperative binding of two Ca(2+) per ATPase (SERCA) occurs within the membrane-bound region of the enzyme. Direct measurements of binding at various Ca(2+) concentrations demonstrate that site-directed mutations within this region interfere selectively with Ca(2+) occupancy of either one or both bin...

journal_title：Biophysical journal

authors： Inesi G,Zhang Z,Lewis D

更新日期：2002-11-01 00:00:00

abstract：:Kinesin is a motor protein that uses the energy derived from the hydrolysis of ATP to power the transport of organelles along microtubules. To probe the mechanism of this chemical-to-mechanical energy transduction reaction, the movement of microtubules across glass surfaces coated with kinesin was perturbed by raising...

journal_title：Biophysical journal

authors： Hunt AJ,Gittes F,Howard J

更新日期：1994-08-01 00:00:00

abstract：:Pure bending of a membrane bilayer is developed including different properties for each membrane half. Both connected and unconnected bilayer surfaces are treated. The bilayer bending resistance is the resultant of parallel surface compression "resistances." The neutral surface is a function of the upper and lower sur...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1974-12-01 00:00:00

abstract：:We describe a puffing method for changing solutions near one surface of lipid bilayers that allows simultaneous measurement of channel activity and extent of solution change at the bilayer surface. Ion adsorption to the lipid headgroups and screening of the bilayer surface charge by mobile ions provided a convenient p...

journal_title：Biophysical journal

authors： Laver DR,Curtis BA

更新日期：1996-08-01 00:00:00

abstract：:Fusion of a vesicle with its target membrane is preceded by tethering or docking. However, the physical mechanism of vesicle-tethering is unknown. To study this mechanism, we used eosinophil secretory granules, which undergo stimulated homotypic fusion events inside the cell during degranulation. Using a dual optical ...

journal_title：Biophysical journal

authors： Valero V,Nevian T,Ho D,Lindau M

更新日期：2008-11-15 00:00:00

abstract：:We study the effect of ergosterol on the physical properties of 1-[(2)H(31)]palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) multibilayers using deuterium nuclear magnetic resonance. NMR spectra were taken as a function of temperature and ergosterol concentration up to 70 mol %. The spectral first moments sh...

journal_title：Biophysical journal

authors： Hsueh YW,Weng CJ,Chen MT,Thewalt J,Zuckermann M

更新日期：2010-04-07 00:00:00

abstract：:Protein/lipid coassembly is an understudied phenomenon that is important to the function of antimicrobial peptides as well as the pathological effects of amyloid. Here, we study the coassembly process of PAP248-286, a seminal peptide that displays both amyloid-forming and antimicrobial activity. PAP248-286 is a peptid...

journal_title：Biophysical journal

authors： Vane EW,He S,Maibaum L,Nath A

更新日期：2020-09-01 00:00:00

abstract：:Exposure of aqueous suspensions of fragments of the purple membrane of Halobacterium halobium to electric field pulses leads to transient linear dichroism phenomena. The effects are interpreted in terms of field-induced alignments of the bacteriorhodopsin chromophore. Two observed relaxation times (tau) are attributed...

journal_title：Biophysical journal

authors： Shinar R,Druckmann S,Ottolenghi M,Korenstein R

更新日期：1977-07-01 00:00:00

abstract：:Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates ...

journal_title：Biophysical journal

authors： Hu J,Asbury T,Achuthan S,Li C,Bertram R,Quine JR,Fu R,Cross TA

更新日期：2007-06-15 00:00:00

abstract：:There are several examples of membrane-associated protein domains that target curved membranes. This behavior is believed to have functional significance in a number of essential pathways, such as clathrin-mediated endocytosis, which involve dramatic membrane remodeling and require the recruitment of various cofactors...

journal_title：Biophysical journal

authors： Cui H,Lyman E,Voth GA

更新日期：2011-03-02 00:00:00

abstract：:A theory of molecular motors is presented that explains how the energy released in single chemical reactions can generate mechanical motion and force. In the simplest case the fluctuating movements of a motor enzyme are well described by a diffusion process on a two-dimensional potential energy surface, where one dime...

journal_title：Biophysical journal

authors： Keller D,Bustamante C

更新日期：2000-02-01 00:00:00

abstract：:Cationic liposomes (CLs) are used worldwide as gene vectors (carriers) in nonviral clinical applications of gene delivery, albeit with unacceptably low transfection efficiencies (TE). We present three-dimensional laser scanning confocal microscopy studies revealing distinct interactions between CL-DNA complexes, for b...

journal_title：Biophysical journal

authors： Lin AJ,Slack NL,Ahmad A,George CX,Samuel CE,Safinya CR

更新日期：2003-05-01 00:00:00

abstract：:Myosin subfragment 1 (S1) can be specifically modified at Lys-553 with the fluorescent probe FHS (6-[fluorescein-5(and 6)-carboxamido]hexanoic acid succinimidyl ester) (Bertrand, R., J. Derancourt, and R. Kassab. 1995. Biochemistry. 34:9500-9507), and solvent quenching of FHS-S1 with iodide has been shown to be sensit...

journal_title：Biophysical journal

authors： Cooper WC,Chrin LR,Berger CL

更新日期：2000-03-01 00:00:00

abstract：:Artificial membrane systems allow researchers to study the structure and function of membrane proteins in a matrix that approximates their natural environment and to integrate these proteins in ex vivo devices such as electronic biosensors, thin-film protein arrays, or biofuel cells. Given that most membrane proteins ...

journal_title：Biophysical journal

authors： Tunuguntla R,Bangar M,Kim K,Stroeve P,Ajo-Franklin CM,Noy A

更新日期：2013-09-17 00:00:00

abstract：:Pyranine (8-hydroxy pyrene-1,3,6-trisulfonate) is a commonly used photoacid that discharges a proton when excited to its first electronic singlet state. Follow-up of its dissociation kinetics reveals the physicochemical properties of its most immediate environment. At vanishing ionic strength the dye adsorbs to the Es...

journal_title：Biophysical journal

authors： Nachliel E,Pollak N,Huppert D,Gutman M

更新日期：2001-03-01 00:00:00

abstract：:Analyses of receptor-ligand interactions are important to the understanding of cellular adhesion. Traditional methods of measuring the three-dimensional (3D) dissociation constant (Kd) require at least one of the molecular species in solution and hence cannot be directly applied to the case of cell adhesion. We descri...

journal_title：Biophysical journal

authors： Piper JW,Swerlick RA,Zhu C

更新日期：1998-01-01 00:00:00

abstract：:A first principles model for calculating hydrogen bonding interactions, previously applied to water, is here applied to the more difficult problem of interactions between DNA bases. We first consider the energetics and geometry for the A-T and the G-C basepairs, comparing our results to other calculated results as wel...

journal_title：Biophysical journal

authors： Lewis JP,Sankey OF

更新日期：1995-09-01 00:00:00

abstract：:The strength of receptor-mediated cell adhesion is directly controlled by the mechanism of cohesive failure between the cell surface and underlying substrate. Unbinding can occur either at the locus of the specific bond or within the bilayer, which results in tearing the hydrophobic anchors from the membrane interior....

journal_title：Biophysical journal

authors： Leckband D,Müller W,Schmitt FJ,Ringsdorf H

更新日期：1995-09-01 00:00:00

abstract：:Alterations in bone tissue composition during osteoporosis likely disrupt the mechanical environment of bone cells and may thereby initiate a mechanobiological response. It has proved challenging to characterize the mechanical environment of bone cells in vivo, and the mechanical environment of osteoporotic bone cells...

journal_title：Biophysical journal

authors： Verbruggen SW,Mc Garrigle MJ,Haugh MG,Voisin MC,McNamara LM

更新日期：2015-04-07 00:00:00

abstract：:Ca2+ release from the endoplasmic reticulum (ER) contributes to Ca2+ transients in frog sympathetic ganglion neurons. Here we use video-rate confocal fluo-4 fluorescence imaging to show that single action potentials reproducibly trigger rapidly rising Ca2+ transients at 1-3 local hot spots within the peripheral ER-ric...

journal_title：Biophysical journal

authors： Cseresnyés Z,Schneider MF

更新日期：2004-01-01 00:00:00

abstract：:N-BAR domains are protein modules that bind to and induce curvature in membranes via a charged concave surface and N-terminal amphipathic helices. Recently, molecular dynamics simulations have demonstrated that the N-BAR domain can induce a strong local curvature that matches the curvature of the BAR domain surface fa...

journal_title：Biophysical journal

authors： Blood PD,Swenson RD,Voth GA

更新日期：2008-08-01 00:00:00

abstract：:A novel combined approach of molecular dynamics (MD) and Monte Carlo simulations is developed to calculate various free-volume distributions as a function of position in a lipid bilayer membrane at 323 K. The model bilayer consists of 2 x 100 chain molecules with each chain molecule having 15 carbon segments and one h...

journal_title：Biophysical journal

authors： Xiang TX

更新日期：1993-09-01 00:00:00

abstract：:The following arguments are presented for the observation that curves relating free Ca2+ and force development of thin filament regulated myofilaments of skinned muscle fibers have Hill coefficient (n) greater than 4, which is the number of Ca2+ binding sites on troponin: Activation of the myofilaments is a process re...

journal_title：Biophysical journal

authors： Shiner JS,Solaro RJ

更新日期：1984-10-01 00:00:00

abstract：:In the photoactive yellow protein, PYP, both Glu46 and Tyr42 form hydrogen bonds to the phenolic OH group of the p-hydroxycinnamoyl chromophore. Previous work on replacement of the carboxyl group of Glu46 by an amide group (Glu46Gln) has shown that changing the nature of this hydrogen bond has a minimal effect on the ...

journal_title：Biophysical journal

authors： Devanathan S,Lin S,Cusanovich MA,Woodbury N,Tollin G

更新日期：2001-10-01 00:00:00

abstract：:We aimed to investigate insulin amyloid fibril polymorphism caused by salt effects and heating temperature and to visualize the structural differences of the polymorphisms in situ using Raman imaging without labeling. The time course monitoring for amyloid formation was carried out in an acidic condition without any s...

journal_title：Biophysical journal

authors： Ishigaki M,Morimoto K,Chatani E,Ozaki Y

更新日期：2020-06-16 00:00:00

abstract：:Mathematical modeling of the cardiac action potential has proven to be a powerful tool for illuminating various aspects of cardiac function, including cardiac arrhythmias. However, no currently available detailed action potential model accurately reproduces the dynamics of the cardiac action potential and intracellula...

journal_title：Biophysical journal

authors： Mahajan A,Shiferaw Y,Sato D,Baher A,Olcese R,Xie LH,Yang MJ,Chen PS,Restrepo JG,Karma A,Garfinkel A,Qu Z,Weiss JN

更新日期：2008-01-15 00:00:00

abstract：:A system based on two designed peptides, namely the cationic peptide K, (KIAALKE)3, and its complementary anionic counterpart called peptide E, (EIAALEK)3, has been used as a minimal model for membrane fusion, inspired by SNARE proteins. Although the fact that docking of separate vesicle populations via the formation ...

journal_title：Biophysical journal

authors： Rabe M,Aisenbrey C,Pluhackova K,de Wert V,Boyle AL,Bruggeman DF,Kirsch SA,Böckmann RA,Kros A,Raap J,Bechinger B

更新日期：2016-11-15 00:00:00