The hill coefficient for the Ca2+-activation of striated muscle contraction.

abstract：:Nonconfluent fibroblasts are relatively depolarized when compared with confluent fibroblasts, and transient hyperpolarizations result from a range of external stimuli as well as internal cellular activities. This electrical activity ceases, along with growth and mitogenic activity, when the cells become confluent. A c...

journal_title：Biophysical journal

authors： Stockbridge LL,French AS

更新日期：1988-07-01 00:00:00

abstract：:The folding of short alanine-based peptides with different numbers of lysine residues is simulated at constant temperature (274 K) using the rigid-element Monte Carlo method. The solvent-referenced potential has prevented the multiple-minima problem in helix folding. From various initial structures, the peptides with ...

journal_title：Biophysical journal

authors： Sung SS

更新日期：1995-03-01 00:00:00

abstract：:A voltage clamp study has been performed in the presynaptic terminal of the squid stellate ganglion. After blockage of the voltage-dependent sodium and potassium conductances, an inward calcium current is demonstrated. Given a step-depolarization pulse, this voltage- and time-dependent conductance has an S-shaped onse...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:We present a theoretical and experimental analysis of the effects of nonlinear flow in a cone-plate viscometer. The analysis predicts that flow in the viscometer is a function of two parameters, the Reynolds number and the cone angle. Nonlinear flow occurs at high shear rates and causes spatial variations in wall shea...

journal_title：Biophysical journal

authors： Shankaran H,Neelamegham S

更新日期：2001-06-01 00:00:00

abstract：:The conduction properties of ClC-0 and ClC-1 chloride channels are examined using electrostatic calculations and three-dimensional Brownian dynamics simulations. We create an open-state configuration of the prokaryotic ClC Cl(-) channel using its known crystallographic structure as a basis. Two residues that are occlu...

journal_title：Biophysical journal

authors： Corry B,O'Mara M,Chung SH

更新日期：2004-02-01 00:00:00

abstract：:The patch-clamp technique of Mueller (1975, Ann. N.Y. Acad. Sci., 274:247-264) and Neher and Sakmann (1976, Nature (Lond.), 260:799-802) was modified to be suitable for single-channel measurements in lipid bilayers at potentials up to 500 mV. This method was used to study gramicidin A single-channel current-voltage ch...

journal_title：Biophysical journal

authors： Andersen OS

更新日期：1983-02-01 00:00:00

abstract：:Crystallographic structure and deuterium accessibility comparisons of CcO in different redox states have suggested conformational changes of mechanistic significance. To predict the intrinsic flexibility and low energy motions in CcO, this work has analyzed available high-resolution crystallographic structures with Pr...

journal_title：Biophysical journal

authors： Buhrow L,Ferguson-Miller S,Kuhn LA

更新日期：2012-05-02 00:00:00

abstract：:The effects of low pH, and of alkaline earth cations, were examined on calcium uptake by pinched-off nerve terminals (synaptosomes). This uptake appears to be mediated by voltage-sensitive Ca channels (J. Physiol. 247:617, 1975). Ca uptake was measured in low (5 mM) or high (77 mM) potassium media. The extra uptake pr...

journal_title：Biophysical journal

authors： Nachshen DA,Blaustein MP

更新日期：1979-05-01 00:00:00

abstract：:KATP channels assemble from four regulatory SUR1 and four pore-forming Kir6.2 subunits. At the single-channel current level, ATP-dependent gating transitions between the active burst and the inactive interburst conformations underlie inhibition of the KATP channel by intracellular ATP. Previously, we identified a slow...

journal_title：Biophysical journal

authors： Drain P,Geng X,Li L

更新日期：2004-04-01 00:00:00

abstract：:A computational analysis of d(GGGGTTTTGGGG)(2) guanine quadruplexes containing either lateral or diagonal four-thymidine loops was carried out using molecular dynamics (MD) simulations in explicit solvent, locally enhanced sampling (LES) simulations, systematic conformational search, and free energy molecular-mechanic...

journal_title：Biophysical journal

authors： Fadrná E,Spacková N,Stefl R,Koca J,Cheatham TE 3rd,Sponer J

更新日期：2004-07-01 00:00:00

abstract：:We present a quantitative analysis of conformational changes of the nucleotide-binding subunits, MalK(2), of the maltose ATP-binding cassette importer MalFGK(2) during the transport cycle. Distance changes occurring between selected residues were monitored in the full transporter by site-directed spin-labeling electro...

journal_title：Biophysical journal

authors： Grote M,Bordignon E,Polyhach Y,Jeschke G,Steinhoff HJ,Schneider E

更新日期：2008-09-15 00:00:00

abstract：:In ubiquitination, cullin-RING E3 ubiquitin ligases (CRLs) assist in ubiquitin transfer from ubiquitin-conjugating enzyme E2 to the substrate. Neddylation, which involves NEDD8 transfer from E2 to E3-cullin, stimulates ubiquitination by inducing conformational change in CRLs. However, deneddylation, which removes NEDD...

journal_title：Biophysical journal

authors： Liu J,Nussinov R

更新日期：2010-08-04 00:00:00

abstract：:Understanding cellular membrane processes is critical for the study of events such as viral entry, neurotransmitter exocytosis, and immune activation. Supported lipid bilayers are commonly used to model these membrane processes experimentally. Despite the relative simplicity of such a system, many important structural...

journal_title：Biophysical journal

authors： Kasson PM,Pande VS

更新日期：2004-06-01 00:00:00

abstract：:Changes in the ultraviolet light scattering from a suspension of purple membrane fragments were detected during the photocycle of bacteriorhodopsin with a cross-correlation method. The scattered light intensity from a suspension of membrane fragments containing the protein bacteriorhodopsin was measured on a logarithm...

journal_title：Biophysical journal

authors： Czégé J,Reinisch L

更新日期：1990-09-01 00:00:00

abstract：:The amyloid fibrillar form of the protein Tau is involved in a number of neurodegenerative diseases, also known as tauopathies. In this work, six different fibrillar Tau isoforms were assembled in vitro. The morphological and nanomechanical properties of these isoforms were studied using atomic force microscopy at hig...

journal_title：Biophysical journal

authors： Makky A,Bousset L,Madiona K,Melki R

更新日期：2020-12-15 00:00:00

abstract：:A promising strategy to control the aggregation of the Alzheimer's Aβ peptide in the brain is the clearance of Aβ from the central nervous system into the peripheral blood plasma. Among plasma proteins, human serum albumin plays a critical role in the Aβ clearance to the peripheral sink by binding to Aβ oligomers and ...

journal_title：Biophysical journal

authors： Milojevic J,Melacini G

更新日期：2011-01-05 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:We report applications of two-photon excitation fluorescence (2PEF) microscopy with subdiffraction-limit resolution for green-fluorescent-protein-tagged cell imaging. The microscope integrates 2PEF microscopy and stimulated emission depletion microscopy in one microscope that has the benefits of both techniques: intri...

journal_title：Biophysical journal

authors： Li Q,Wu SS,Chou KC

更新日期：2009-12-16 00:00:00

abstract：:Mechanical properties of living cells can be determined using atomic force microscopy (AFM). In this study, a novel analysis was developed to determine the mechanical properties of adherent monolayers of pulmonary microvascular endothelial cells (ECs) using AFM and finite element modeling, which considers both the fin...

journal_title：Biophysical journal

authors： Kang I,Panneerselvam D,Panoskaltsis VP,Eppell SJ,Marchant RE,Doerschuk CM

更新日期：2008-04-15 00:00:00

abstract：:Prion diseases are infectious fatal neurodegenerative diseases including Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. The misfolding and conversion of cellular PrP in such mammals into pathogenic PrP is believed to be the key procedure. Rabbits are among the few mammalian species...

journal_title：Biophysical journal

authors： Zhou Z,Yan X,Pan K,Chen J,Xie ZS,Xiao GF,Yang FQ,Liang Y

更新日期：2011-09-21 00:00:00

abstract：:The binding mechanism of sweet proteins to their receptor, a G-protein-coupled receptor, is not supported by direct structural information. In principle, the key groups responsible for biological activity (glucophores) can be localized on a small structural unit (sweet finger) or spread on a larger surface area. A rec...

journal_title：Biophysical journal

authors： De Simone A,Spadaccini R,Temussi PA,Fraternali F

更新日期：2006-05-01 00:00:00

abstract：:Surface and subsurface dynamics of Rat Basophilic Leukemia cells, a model system of stimulated secretion, were imaged using Scanning Force Microscopy (SFM) at a rate of 50-60 s/image. Cytoskeletal elements and organelles were tracked within quiescent cells and those activated after IgE receptor crosslinking. In additi...

journal_title：Biophysical journal

authors： Braunstein D,Spudich A

更新日期：1994-05-01 00:00:00

abstract：:A new kinetic model of the Na(+)/H(+) exchanger (NHE) was developed by fitting a variety of major experimental findings, such as ion-dependencies, forward/reverse mode, and the turnover rate. The role of NHE in ion homeostasis was examined by implementing the NHE model in a minimum cell model including intracellular p...

journal_title：Biophysical journal

authors： Cha CY,Oka C,Earm YE,Wakabayashi S,Noma A

更新日期：2009-11-18 00:00:00

abstract：:Interactions between the graft copolymer poly(L-lysine)-g-poly(ethylene glycol), PLL-g-PEG, and two kinds of surface-supported lipidic systems (supported phospholipid bilayers and supported vesicular layers) were investigated by a combination of microscopic and spectroscopic techniques. It was found that the applicati...

journal_title：Biophysical journal

authors： Rossetti FF,Reviakine I,Csúcs G,Assi F,Vörös J,Textor M

更新日期：2004-09-01 00:00:00

abstract：:Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These resu...

journal_title：Biophysical journal

authors： Sikorav JL,Pelta J,Livolant F

更新日期：1994-10-01 00:00:00

abstract：:Microstructured surfaces provide a unique framework to probe cell migration and cytoskeletal dynamics in a standardized manner. Here, we report on the steady-state occupancy probability of cells in asymmetric two-state microstructures that consist of two fibronectin-coated adhesion sites connected by a thin guidance c...

journal_title：Biophysical journal

authors： Fink A,Brückner DB,Schreiber C,Röttgermann PJF,Broedersz CP,Rädler JO

更新日期：2020-02-04 00:00:00

abstract：:Applying self-consistent field theory, we consider a coarse-grained model for the polymerlike projections of neurofilament (NF) proteins that form a brush structure around neurofilaments. We focus on effects of molecular composition, which is the relative occurrence of NF-H, NF-M, and NF-L proteins, on the organizatio...

journal_title：Biophysical journal

authors： Zhulina EB,Leermakers FA

更新日期：2010-02-03 00:00:00

abstract：:At low surface concentrations that permit formation of impermeable membranes, neuronal soluble N-ethyl maleimide sensitive factor attachment protein receptor (SNARE) proteins form a stable, parallel, trans complex when vesicles are brought into contact by a low concentration of poly(ethylene glycol) (PEG). Surprisingl...

journal_title：Biophysical journal

authors： Dennison SM,Bowen ME,Brunger AT,Lentz BR

更新日期：2006-03-01 00:00:00

abstract：:Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was develope...

journal_title：Biophysical journal

authors： Sengupta K,Aranda-Espinoza H,Smith L,Janmey P,Hammer D

更新日期：2006-12-15 00:00:00