Abstract:
:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary distribution of the probe orientation is of a Gaussian type. For the same experimental anisotropy decay, analysis by the Gaussian model predicts a smaller value for the rate of wobbling motion than the strict cone analysis, but the difference is 35% at most; the cone angle obtained by the strict cone analysis agrees closely with the effective width of the Gaussian distribution. The results suggest that, when only two parameters (the rate and the angular range) are extracted from an experiment, the choice of a model is not crucial as long as the model contains the essential feature, e.g., the more-or-less conical restriction, of the motion under study. Model-independent analyses are also discussed.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Kinosita K Jr,Ikegami A,Kawato Sdoi
10.1016/S0006-3495(82)84692-4subject
Has Abstractpub_date
1982-02-01 00:00:00pages
461-4issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(82)84692-4journal_volume
37pub_type
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