Lipid Droplets Can Spontaneously Bud Off from a Symmetric Bilayer.

abstract：:Internally perfused squid giant axons with intact sodium inactivation gating were prepared for gating current experiments. Gating current records were obtained in sinusoidally driven dynamic steady states and as dynamic transients as functions of the mean membrane potential and the frequency of the command sinusoid. C...

journal_title：Biophysical journal

authors： Fohlmeister JF,Adelman WJ Jr

更新日期：1986-09-01 00:00:00

abstract：:Microscopic calcium (Ca2+) events (such as Ca2+ sparks) are an important area for study, as they help clarify the mechanism(s) underlying intracellular signaling. In the heart, Ca2+ sparks occur as a result of Ca2+ release from the sarcoendoplasmic reticulum, via ryanodine receptor channels. Measurement of Ca2+ spark ...

journal_title：Biophysical journal

authors： Kong CH,Soeller C,Cannell MB

更新日期：2008-12-15 00:00:00

abstract：:T cell receptor phosphorylation by Lck is an essential step in T cell activation. It is known that the conformational states of Lck control enzymatic activity; however, the underlying principles of how Lck finds its substrate over the plasma membrane remain elusive. Here, single-particle tracking is paired with photoa...

journal_title：Biophysical journal

authors： Hilzenrat G,Pandžić E,Yang Z,Nieves DJ,Goyette J,Rossy J,Ma Y,Gaus K

更新日期：2020-03-24 00:00:00

abstract：:Some low molecular weight biomolecules, i.e., NAD(P)H, are unstable at high temperatures. The use of these biomolecules by thermophilic microorganisms has been scarcely analyzed. Herein, NADH stability has been studied at different temperatures and viscosities. NADH decay increased at increasing temperatures. At incre...

journal_title：Biophysical journal

authors： Cuecas A,Cruces J,Galisteo-López JF,Peng X,Gonzalez JM

更新日期：2016-08-23 00:00:00

abstract：:Calmodulin (CaM) is a highly flexible calcium-binding protein that mediates signal transduction through an ability to differentially bind to highly variable binding sequences in target proteins. To identify how binding affects CaM motions, and its relationship to conformational entropy and target peptide sequence, we ...

journal_title：Biophysical journal

authors： Smith DM,Straatsma TP,Squier TC

更新日期：2012-10-03 00:00:00

abstract：:To study why pancreatic beta-cells prefer to burst as a multi-cellular complex, we have formulated a stochastic model for bursting clusters of excitable cells. Our model incorporated a delayed rectifier K+ channel, a fast voltage-gated Ca2+ channel, and a slow Cai-blockable Ca2+ channel. The fraction of ATP-sensitive ...

journal_title：Biophysical journal

authors： Chay TR,Kang HS

更新日期：1988-09-01 00:00:00

abstract：:Deciphering the mechanisms underlying the high sensitivity of cells to mechanical microenvironments is crucial for understanding many physiological and pathological processes, e.g., stem cell differentiation and cancer cell metastasis. Here, a cytoskeletal tensegrity model is proposed to study the reorientation of pol...

journal_title：Biophysical journal

authors： Xu GK,Li B,Feng XQ,Gao H

更新日期：2016-10-04 00:00:00

abstract：:Dual-trap optical tweezers are often used in high-resolution measurements in single-molecule biophysics. Such measurements can be hindered by the presence of extraneous noise sources, the most prominent of which is the coupling of fluctuations along different spatial directions, which may affect any optical tweezers s...

journal_title：Biophysical journal

authors： Ribezzi-Crivellari M,Ritort F

更新日期：2012-11-07 00:00:00

abstract：:A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we suggest that this motif maximizes information flow in a noisy network. Gene expressio...

journal_title：Biophysical journal

authors： Hormoz S

更新日期：2013-03-05 00:00:00

abstract：:We study theoretically the feasibility of using transverse electronic transport within a nanopore for rapid DNA sequencing. Specifically, we examine the effects of the environment and detection probes on the distinguishability of the DNA bases. We find that the intrinsic measurement bandwidth of the electrodes helps t...

journal_title：Biophysical journal

authors： Lagerqvist J,Zwolak M,Di Ventra M

更新日期：2007-10-01 00:00:00

abstract：:Conduction speed (theta) in single myelinated Rana pipiens sciatic nerve fibers has been precisely measured using intracellular recording and on-line digital computer techniques. The dependence of relative speed on external Na concentration at 15 degrees C has been found to be ln(theta(1)/theta(2)) = 0.524 (+/-0.018) ...

journal_title：Biophysical journal

authors： Hardy WL

更新日期：1973-10-01 00:00:00

abstract：:We investigate the interactions between lipid bilayers and amphiphilic peptides using a solvent-free coarse-grained simulation technique. In our model, each lipid is represented by one hydrophilic and three hydrophobic beads. The amphiphilic peptide is modeled as a hydrophobic-hydrophilic cylinder with hydrophilic cap...

journal_title：Biophysical journal

authors： Illya G,Deserno M

更新日期：2008-11-01 00:00:00

abstract：:A mean-field statistical mechanical theory has been developed to describe molecular distributions in interphases. The excluded volume interaction has been modeled in terms of a reversible work that is required to create a cavity of the solute size against a pressure tensor exerted by the surrounding interphase molecul...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1994-03-01 00:00:00

abstract：:Clostridium difficile (C. diff) is one of the most common and most severe hospital-acquired infections; its consequences range from lengthened hospital stay to outright lethality. C. diff causes cellular damage through the action of two large toxins TcdA and TcdB. Recently, there has been increased effort toward devel...

journal_title：Biophysical journal

authors： Swett RJ,Cisneros GA,Feig AL

更新日期：2013-07-16 00:00:00

abstract：:Current methods for fluorescence resonance energy transfer (FRET) microscopy of living cells involve taking a series of images with alternating excitation colors in separate camera exposures. Here we present a new FRET method based on polarization that requires only one camera exposure and thereby offers the possibili...

journal_title：Biophysical journal

authors： Mattheyses AL,Hoppe AD,Axelrod D

更新日期：2004-10-01 00:00:00

abstract：:Human immunodeficiency virus-1 viral infectivity factor (Vif) is an intrinsically disordered protein responsible for the ubiquitination of the APOBEC3 (A3) antiviral proteins. Vif folds when it binds Cullin-RING E3 ligase 5 and the transcription cofactor CBF-β. A five-protein complex containing the substrate receptor ...

journal_title：Biophysical journal

authors： Ball KA,Chan LM,Stanley DJ,Tierney E,Thapa S,Ta HM,Burton L,Binning JM,Jacobson MP,Gross JD

更新日期：2019-04-23 00:00:00

abstract：:Changes in voltage-dependent gating represent a common pathogenetic mechanism for genetically inherited channelopathies, such as benign familial neonatal seizures or peripheral nerve hyperexcitability caused by mutations in neuronal K(v)7.2 channels. Mutation-induced changes in channel voltage dependence are most ofte...

journal_title：Biophysical journal

authors： Miceli F,Vargas E,Bezanilla F,Taglialatela M

更新日期：2012-03-21 00:00:00

abstract：:A major challenge in synthetic gene delivery is to quantitatively predict the optimal design of polymer-based gene carriers (polyplexes). Here, we report a consistent, integrated, and fundamentally grounded computational methodology to address this challenge. This is achieved by accurately representing the spatio-temp...

journal_title：Biophysical journal

authors： Dinh AT,Pangarkar C,Theofanous T,Mitragotri S

更新日期：2007-02-01 00:00:00

abstract：:Biological membranes composed of lipids and proteins are in contact with electrolytes like aqueous NaCl solutions. Based on molecular dynamics studies it is widely believed that Na(+) ions specifically bind to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes, whereas Cl(-) ions stay in solution. Here,...

journal_title：Biophysical journal

authors： Knecht V,Klasczyk B

更新日期：2013-02-19 00:00:00

abstract：:Myofilament length-dependent activation is a universal property of striated muscle, yet the molecular mechanisms that underlie this phenomenon are incompletely understood. Additionally, the rate by which sarcomere length (SL) is sensed and then transduced to form length-dependent activation is unknown. Here, using iso...

journal_title：Biophysical journal

authors： Mateja RD,de Tombe PP

更新日期：2012-07-03 00:00:00

abstract：:The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that FR-...

journal_title：Biophysical journal

authors： Chen H,Yang J,Low PS,Cheng JX

更新日期：2008-02-15 00:00:00

abstract：:The kinetics of conformational changes of P-type ATPases necessary for the occlusion or deocclusion of transported ions are known to be sensitive to the composition of the surrounding membrane, e.g., phospholipid content, mole percentage of cholesterol, and the presence of lipid-bound anions. Research has shown that m...

journal_title：Biophysical journal

authors： Clarke RJ

更新日期：2015-10-20 00:00:00

abstract：:P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of intracellular materials from the damaged cells. To explore the nature of the interaction between P188 and cell membranes, we have constructed a model...

journal_title：Biophysical journal

authors： Maskarinec SA,Hannig J,Lee RC,Lee KY

更新日期：2002-03-01 00:00:00

abstract：:Using a model for catalysis of a dynamic equilibrium, the role of constraint in catalysis is quantified. The intrinsic rigidity of proteins is shown to be insufficient to constrain the activated complexes of enzymes, irrespective of the mechanism. However, when minimization of the surface excess free energy of water s...

journal_title：Biophysical journal

authors： Vanselow DG

更新日期：2002-05-01 00:00:00

abstract：:Coupled folding-binding is central to the function of many intrinsically disordered proteins, yet not fully understood. With a continuous three-letter protein model, we explore the free-energy landscape of pairs of interacting sequences and how it is impacted by 1), variations in the binding mechanism; and 2), the add...

journal_title：Biophysical journal

authors： Bhattacherjee A,Wallin S

更新日期：2012-02-08 00:00:00

abstract：:With the aim of understanding the relation between structure and gating of CNGA1 channels from bovine rod, an extensive cysteine scanning mutagenesis was performed. Each residue from Phe-375 to Val-424 was mutated into a cysteine one at a time and the modification caused by various sulfhydryl reagents was analyzed. Th...

journal_title：Biophysical journal

authors： Nair AV,Mazzolini M,Codega P,Giorgetti A,Torre V

更新日期：2006-05-15 00:00:00

abstract：:To understand better the effect of electrostatics on the rigidity of the DNA double helix, we define DNA*, the null isomer of DNA, as the hypothetical structure that would result from DNA if its phosphate groups were not ionized. For the purposes of theoretical analysis, we model DNA* as identical to ordinary DNA but ...

journal_title：Biophysical journal

authors： Manning GS

更新日期：2006-11-15 00:00:00

abstract：:Gangliosides are a class of glycosphingolipids (GSLs) with amphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domains makes them vital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustere...

journal_title：Biophysical journal

authors： Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

更新日期：2016-11-01 00:00:00

abstract：:The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe to determine the viscous properties of the aqueous lumen of the ER. GFP was targeted to the ER lumen of CHO cells by transient tr...

journal_title：Biophysical journal

authors： Dayel MJ,Hom EF,Verkman AS

更新日期：1999-05-01 00:00:00

abstract：:Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescen...

journal_title：Biophysical journal

authors： Swift JL,Cramb DT

更新日期：2008-07-01 00:00:00