Photoperturbation of the heme a3-CuB binuclear center of cytochrome c oxidase CO complex observed by Fourier transform infrared spectroscopy.

abstract：:Dithioation of DNA phosphate is known to enhance binding affinities, at least for some proteins. We mechanistically characterized this phenomenon for the Antennapedia homeodomain-DNA complex by integrated use of fluorescence, isothermal titration calorimetry, NMR spectroscopy, and x-ray crystallography. By fluorescenc...

journal_title：Biophysical journal

authors： Zandarashvili L,Nguyen D,Anderson KM,White MA,Gorenstein DG,Iwahara J

更新日期：2015-09-01 00:00:00

abstract：:To record post synaptic potentials or electrical activity from processes of single cells in a central nervous system (CNS) preparation in situ, voltage sensitive dyes can be injected intracellularly, thereby staining only the cell under investigation. We report the structure, evaluation, and synthesis of 11 fluorescen...

journal_title：Biophysical journal

authors： Grinvald A,Salzberg BM,Lev-Ram V,Hildesheim R

更新日期：1987-04-01 00:00:00

abstract：:A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonl...

journal_title：Biophysical journal

authors： Gratton E,Limkeman M

更新日期：1983-12-01 00:00:00

abstract：:Extracellular vesicles (EVs) are proposed to play important roles in intercellular communication. Two classes of EVs can be distinguished based on their intracellular origin. Exosomes are generated within endosomes and released when these fuse with the plasma membrane, whereas ectosomes bud directly from the plasma me...

journal_title：Biophysical journal

authors： Jackson CE,Scruggs BS,Schaffer JE,Hanson PI

更新日期：2017-09-19 00:00:00

abstract：:Adaptation of the attractant response in Escherichia coli is attributable to the methylation of its transmembrane chemotactic receptors by the methyltransferase CheR. This protein contains two binding domains, one for the sites of methylation themselves and the other for a flexible tether at the C terminus of the rece...

journal_title：Biophysical journal

authors： Levin MD,Shimizu TS,Bray D

更新日期：2002-04-01 00:00:00

abstract：:A computational analysis of d(GGGGTTTTGGGG)(2) guanine quadruplexes containing either lateral or diagonal four-thymidine loops was carried out using molecular dynamics (MD) simulations in explicit solvent, locally enhanced sampling (LES) simulations, systematic conformational search, and free energy molecular-mechanic...

journal_title：Biophysical journal

authors： Fadrná E,Spacková N,Stefl R,Koca J,Cheatham TE 3rd,Sponer J

更新日期：2004-07-01 00:00:00

abstract：:A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...

journal_title：Biophysical journal

authors： Paillotin G,Geacintov NE,Breton J

更新日期：1983-10-01 00:00:00

abstract：:Neal and Cunningham (Neal, M. J., and J. R. Cunningham. 1995. J. Physiol. (Lond.). 482:363-372) showed that GABA(B) agonists and glycinergic antagonists enhance the light-evoked release of retinal acetylcholine. They proposed that glycinergic cells inhibit the cholinergic Starburst amacrine cells and are in turn inhib...

journal_title：Biophysical journal

authors： Grzywacz NM,Zucker CL

更新日期：2006-07-15 00:00:00

abstract：:Saturation transfer electron paramagnetic resonance (ST-EPR) spectroscopy has been employed to characterize the very slow microsecond to millisecond rotational dynamics of a wide range of nitroxide spin-labeled proteins and other macromolecules in the past three decades. The vast majority of this previous work has bee...

journal_title：Biophysical journal

authors： Hustedt EJ,Beth AH

更新日期：2004-06-01 00:00:00

abstract：:Solid-state NMR study shows that the 22-residue K3 peptide (Ser(20)-Lys(41)) from beta(2)-microglobulin (beta(2)m) adopts a beta-strand-loop-beta-strand conformation in its fibril state. Residue Pro(32) has a trans conformation in the fibril state of the peptide, while it adopts a cis conformation in the native state ...

journal_title：Biophysical journal

authors： Liang C,Derreumaux P,Mousseau N,Wei G

更新日期：2008-07-01 00:00:00

abstract：:In an attempt to quantitate the physical behavior of biological systems, Fourier analysis has been applied to the respiratory and circulatory systems by a number of investigators. The validity of this application has been questioned on the basis that these systems are nonlinear and not strictly periodic. If these obje...

journal_title：Biophysical journal

authors： Attinger EO,Anné A,McDonald DA

更新日期：1966-05-01 00:00:00

abstract：:The energy transfer processes between carotenoids and Chls have been studied by femtosecond transient absorption in the CP29-WT complex, which contains only two carotenoids per polypeptide located in the L1 and L2 sites, and in the CP29-E166V mutant in which only the L1 site is occupied. The comparison of these two sa...

journal_title：Biophysical journal

authors： Croce R,Müller MG,Caffarri S,Bassi R,Holzwarth AR

更新日期：2003-04-01 00:00:00

abstract：:A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than ...

journal_title：Biophysical journal

authors： Prosser RS,Volkov VB,Shiyanovskaya IV

更新日期：1998-11-01 00:00:00

abstract：:Understanding the intermolecular interaction potential, V(r), of proteins under the influence of temperature, pressure, and salt concentration is essential for understanding protein aggregation, crystallization, and protein phase behavior in general. Here, we report small-angle x-ray scattering studies on dense lysozy...

journal_title：Biophysical journal

authors： Möller J,Schroer MA,Erlkamp M,Grobelny S,Paulus M,Tiemeyer S,Wirkert FJ,Tolan M,Winter R

更新日期：2012-06-06 00:00:00

abstract：:The effects of rigor and cycling cross-bridges on distributions of calcium (Ca) bound within sarcomeres of rabbit psoas muscle fibers were compared using electron probe x-ray microanalysis. Calcium in the overlap region of rigor fibers, after correction for that bound to thick filaments, was significantly higher than ...

journal_title：Biophysical journal

authors： Cantino ME,Quintanilla A

更新日期：2007-01-15 00:00:00

abstract：:In adherent and motile neutrophils NAD(P)H concentration, flavoprotein redox potential, and production of reactive oxygen species and nitric oxide, are all periodic and exhibit defined phase relationships to an underlying metabolic oscillation of approximately 20 s. Utilizing fluorescence microscopy, we have shown in ...

journal_title：Biophysical journal

authors： Rosenspire AJ,Kindzelskii AL,Simon BJ,Petty HR

更新日期：2005-05-01 00:00:00

abstract：:Although ubiquitous, the processes by which bacteria colonize surfaces remain poorly understood. Here we report results for the influence of the wall shear stress on the early-stage adhesion of Pseudomonas aeruginosa PA14 on glass and polydimethylsiloxane surfaces. We use image analysis to measure the residence time o...

journal_title：Biophysical journal

authors： Lecuyer S,Rusconi R,Shen Y,Forsyth A,Vlamakis H,Kolter R,Stone HA

更新日期：2011-01-19 00:00:00

abstract：:We continue in this paper the presentation of theoretical and experimental methods for the joint refinement of neutron and x-ray lamellar diffraction data for the analysis of fluid (L alpha phase) bilayer structure (Wiener, M. C., and S. H. White. 1991 a, b, c. Biophys. J. 59:162-173 and 174-185; Biochemistry. 30:6997...

journal_title：Biophysical journal

authors： Wiener MC,White SH

更新日期：1992-02-01 00:00:00

abstract：:The use of one-dimensional electron density strip models in interpreting low-angle X-ray data from planar and concentric multilayered structures is described. Diffraction formulas for an n-strip model are given. Fourier transforms, normalization constants, and Patterson functions are derived for certain strip models. ...

journal_title：Biophysical journal

authors： Worthington CR

更新日期：1969-02-01 00:00:00

abstract：:A model for the Na-K exchange pump was applied to data on Na(+)-loaded frog sartorius muscle, and was used to relate the rate of adenosine triphosphate (ATP) hydrolysis to the electrical properties of the cell membrane. Membrane hyperpolarization was considered to arise from an electrical current which was produced by...

journal_title：Biophysical journal

authors： Rapoport SI

更新日期：1971-08-01 00:00:00

abstract：:The application of a digital computer to the processing of data from single neurons is described. Examples from experimental data are presented to demonstrate the usefulness of certain types of computations. These methods are placed in a descriptive mathematical framework. Other easily attainable computations are sugg...

journal_title：Biophysical journal

authors： GERSTEIN GL,KIANG NY

更新日期：1960-09-01 00:00:00

abstract：:To understand the thermodynamic exclusion of Na(+) relative to K(+) from the S(2) site of the selectivity filter, the distribution P(X)(epsilon) (X = K(+) or Na(+)) of the binding energy (epsilon) of the ion with the channel is analyzed using the potential distribution theorem. By expressing the excess chemical potent...

journal_title：Biophysical journal

authors： Dixit PD,Merchant S,Asthagiri D

更新日期：2009-03-18 00:00:00

abstract：:Previously, we discussed the calculation of the dipole moments of small proteins using the three-dimensional protein data-base. Our results demonstrate that the calculated dipole moments are in acceptable agreement with measured values. We, however, noted the difficulty of the calculation with larger proteins, in part...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1993-05-01 00:00:00

abstract：:Nanopatterned surfaces are believed to kill bacteria through physical deformation, a mechanism that has immense potential against biochemical resistance. Because of its elusive nature, this mechanism is mostly understood through biophysical modeling. Problematically, accurate descriptions of the contact mechanics and ...

journal_title：Biophysical journal

authors： Velic A,Hasan J,Li Z,Yarlagadda PKDV

更新日期：2021-01-19 00:00:00

abstract：:Odorant-induced currents in mammalian olfactory receptor neurons have proved difficult to obtain reliably using conventional whole-cell recording. By using a mathematical model of the electrical circuit of the patch and rest-of-cell, we demonstrate how cell-attached patch measurements can be used to quantitatively ana...

journal_title：Biophysical journal

authors： Chiu P,Lynch JW,Barry PH

更新日期：1997-03-01 00:00:00

abstract：:Activation of skeletal muscle ryanodine receptors (RyRs) by suramin and disulfonic stilbene derivatives (Diisothiocyanostilbene-2',2'-disulfonic acid (DIDS), 4,4'-dibenzamidostilbene-2,2'-disulfonic acid (DBDS),and 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS)) was investigated using planar bilayers. One reversible...

journal_title：Biophysical journal

authors： O'Neill ER,Sakowska MM,Laver DR

更新日期：2003-03-01 00:00:00

abstract：:Regulation of the F-actin severing activity of gelsolin by Ca2+ has been investigated under physiologic ionic conditions. Tryptophan fluorescence intensity measurements indicate that gelsolin contains at least two Ca2+ binding sites with affinities of 2.5 x 10(7) M-1 and 1.5 x 10(5) M-1. At F-actin and gelsolin concen...

journal_title：Biophysical journal

authors： Kinosian HJ,Newman J,Lincoln B,Selden LA,Gershman LC,Estes JE

更新日期：1998-12-01 00:00:00

abstract：:Comparison of the electron spin resonance spectra of phosphatidylcholines spin-labeled in the sn-2 chain at a position close to the polar region and close to the methyl terminus indicate that symmetrical saturated diacyl phosphatidylcholines with odd and even chain lengths from 13 to 20 C-atoms (and probably also 12 C...

journal_title：Biophysical journal

authors： Swamy MJ,Marsh D

更新日期：1995-10-01 00:00:00

abstract：:Red blood cells aggregate face-to-face to form long, cylindrical, straight chains and sometimes branched structures called rouleaux. Here we extend a kinetic model developed by R. W. Samsel and A. S. Perelson (1982, Biophys. J. 37:493-514) to include both the formation and dissociation of rouleaux. We examine thermody...

journal_title：Biophysical journal

authors： Samsel RW,Perelson AS

更新日期：1984-04-01 00:00:00

abstract：:There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In additio...

journal_title：Biophysical journal

authors： Jiang Y,Rabbi M,Kim M,Ke C,Lee W,Clark RL,Mieczkowski PA,Marszalek PE

更新日期：2009-02-01 00:00:00