Abstract:
:Odorant-induced currents in mammalian olfactory receptor neurons have proved difficult to obtain reliably using conventional whole-cell recording. By using a mathematical model of the electrical circuit of the patch and rest-of-cell, we demonstrate how cell-attached patch measurements can be used to quantitatively analyze responses to odorants or a high (100 mM) K+ solution. High K+ induced an immediate current flux from cell to pipette, which was modeled as a depolarization of approximately 52 mV, close to that expected from the Nernst equation (56 mV), and no change in the patch conductance. By contrast, a cocktail of cAMP-stimulating odorants induced a current flux from pipette into cell following a significant (4-10 s) delay. This was modeled as an average patch conductance increase of 36 pS and a depolarization of 13 mV. Odorant-induced single channels had a conductance of 16 pS. In cells bathed with no Mg2+ and 0.25 mM Ca2+, odorants induced a current flow from cell to pipette, which was modeled as a patch conductance increase of approximately 115 pS and depolarization of approximately 32 mV. All these results are consistent with cAMP-gated cation channels dominating the odorant response. This approach, which provides useful estimates of odorant-induced voltage and conductance changes, is applicable to similar measurements in any small cells.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Chiu P,Lynch JW,Barry PHdoi
10.1016/S0006-3495(97)78791-5subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1442-57issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(97)78791-5journal_volume
72pub_type
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