Abstract:
:Some new approaches to the kinetic study of the reduction of cytochrome aa3 by cytochrome c are presented. The primary innovations are the use of a spectrometer which can acquire multiwavelength data as fast as every 10 microseconds, and the application of a variety of analytical methods which can utilize simultaneously all of the time-resolved spectral data. These techniques include singular value decomposition (SVD), deconvolutions based on pure Gaussian models for absorption peaks, deconvolutions based on isolated absorption spectra for the pure components, and simulations of SVD-deduced and actual experimental difference spectra. The reduction characteristics of the anaerobic resting enzyme can be distinguished from those of pulsed forms. In the former case, only two electrons can be bound by cytochrome aa3, whereas in the latter case complete reduction of the enzyme is achieved.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Hendler RW,Bose SK,Shrager RIdoi
10.1016/S0006-3495(93)81170-6subject
Has Abstractpub_date
1993-09-01 00:00:00pages
1307-17issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(93)81170-6journal_volume
65pub_type
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