Abstract:
:By combining a description of the coupled solute and water flows through the in vivo cornea with a set of appropriate mechanical equilibrium conditions, it is possible to calculate directly the corneal thickness, given the corneal temperature, the state of the aqueous and tears, the swelling pressure-hydration relation of the corneal stroma, and the transport properties of the corneal membranes. Active transport of ions or water by the corneal epithelium or endothelium, or both, are explicitly included. When published parameters are inserted into the formulation, the normal corneal thickness is recovered, and the corneal potential, anteriorly directed water flux, and stromal salt content are in reasonable to quantitative agreement with experiment. The analysis yields a simple physical explanation of the stromal imbibition pressure and the opposing forces which cause the cornea to assume its normal thickness.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Friedman MHdoi
10.1016/S0006-3495(72)86110-1subject
Has Abstractpub_date
1972-06-01 00:00:00pages
648-65issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(72)86110-1journal_volume
12pub_type
杂志文章abstract::Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spa...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.11.001
更新日期:2010-02-17 00:00:00
abstract::In vitro and in situ tests have been run to characterize the reaction of the mettalochromic indicator, arsenazo III, with calcium. Job plots as well as plots of indicator absorbance vs. [Ca2+] at different indicator concentrations show a 1:1 reaction stoichiometry. Equilibrium analysis and analysis using Adair's equat...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(80)85025-9
更新日期:1980-12-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2013.02.022
更新日期:2013-04-02 00:00:00
abstract::Monolayers of a functional pulmonary surfactant (PS) can reach very low surface tensions well below their equilibrium value. The mechanism by which PS monolayers reach such low surface tensions and maintain film stability remains unknown. As shown previously by fluorescence microscopy, phospholipid phase transition an...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.122648
更新日期:2008-05-01 00:00:00
abstract::Erythrocytes were electrofused with multiple rectangular voltage pulses to show an oscillatory movement, divided into swell phases and pump events. During each swell phase, which lasted from 0.5 s to more than 180 s, the fused cells' (doublets') volume increased by colloid osmotic swelling, and the membrane area was e...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(99)77094-3
更新日期:1999-11-01 00:00:00
abstract::We carried out experiments on single isolated myofibrils in which thin filaments had been functionally removed, leaving the connecting (titin) filaments as the sole agent taking up the length change. With technical advances that gave sub-nanometer detectability we examined the time course of single sarcomere-length ch...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)75766-9
更新日期:2001-08-01 00:00:00
abstract::Fluorescence resonance energy transfer (FRET) was used to reveal aspects of the mechanism of signal transduction by epidermal growth factor receptors (EGFR). The superpositions of epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha) and an antibody fragment (29.1) to the carbohydrate extremity of...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(02)75585-9
更新日期:2002-05-01 00:00:00
abstract::Thin filament regulation of contraction is thought to involve the binding of two activating ligands: Ca2+ and strongly bound cross-bridges. The specific cross-bridge states required to promote thin filament activation have not been identified. This study examines the relationship between cross-bridge cycling and thin ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(99)77371-6
更新日期:1999-04-01 00:00:00
abstract::Elastin-derived peptides are released from the extracellular matrix remodeling by numerous proteases and seem to regulate many biological processes, notably cancer progression. The canonical elastin peptide is VGVAPG, which harbors the XGXXPG consensus pattern, allowing interaction with the elastin receptor complex lo...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2020.04.019
更新日期:2020-06-02 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)77751-3
更新日期:1998-12-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)75715-3
更新日期:2001-07-01 00:00:00
abstract::Atomically detailed simulations are used to compute the kinetics of the R-to-T transition in deoxy Scapharca hemoglobin. A computational approach called milestoning is utilized that combines 1), an efficient reaction path algorithm; and 2), a "fragment and glue" approach for classical trajectories. Milestoning compute...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.101899
更新日期:2007-05-01 00:00:00
abstract::The statistical mechanics of cross-bridge action is considered in order to develop constitutive equations that express fiber tension as a function of degree of activation and time history of speed of contraction. The kinetic equation of A.F. Huxley (1) is generalized to apply to the partially activated state. The rate...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/s0006-3495(85)83895-9
更新日期:1985-02-01 00:00:00
abstract::The kinetics of oxygen geminate binding with the taxonomic substates of MbO2 are reported. The maximum entropy method was used to analyze the rebinding kinetics of MbCO and MbO2 monitored in the Soret. The resulting rate distributions were found to consist of a small number of overlapping bands. A global parametric fi...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(02)75561-6
更新日期:2002-04-01 00:00:00
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journal_title:Biophysical journal
pub_type: 信件
doi:10.1016/j.bpj.2010.07.007
更新日期:2010-09-22 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.07.018
更新日期:2009-10-07 00:00:00
abstract::Helix-coil transitions in polyalanine molecules of length 10 are studied by multi-canonical Monte Carlo simulations. The solvation effects are included by either a distance-dependent dielectric permittivity or by a term that is proportional to the solvent-accessible surface area of the peptide. We found a strong depen...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(02)75668-3
更新日期:2002-06-01 00:00:00
abstract::Epigenetic modifications can extend over long genomic regions to form domain-level chromatin states that play critical roles in gene regulation. The molecular mechanism for the establishment and maintenance of these states is not fully understood and remains challenging to study with existing experimental techniques. ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2019.04.006
更新日期:2019-05-21 00:00:00
abstract::Juxtacrine signaling is an important class of signaling systems that plays a crucial role in various developmental processes ranging from coordination of differentiation between neighboring cells to guiding axon growth during neurogenesis. Such signaling systems rely on the interaction between receptors on one cell an...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2014.10.006
更新日期:2014-11-18 00:00:00
abstract::Influenza neuraminidase is an important drug target. Glycans are present on neuraminidase and are generally considered to inhibit antibody binding via their glycan shield. In this work, we studied the effect of glycans on the binding kinetics of antiviral drugs to the influenza neuraminidase. We created all-atom in si...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2020.10.024
更新日期:2020-12-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(02)75189-8
更新日期:2002-07-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(66)86651-1
更新日期:1966-03-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2013.06.043
更新日期:2013-08-06 00:00:00
abstract::The AXH domain of protein Ataxin 1 is thought to play a key role in the misfolding and aggregation pathway responsible for Spinocerebellar ataxia 1. For this reason, a molecular level understanding of AXH oligomerization pathway is crucial to elucidate the aggregation mechanism, which is thought to trigger the disease...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2017.11.025
更新日期:2018-01-23 00:00:00
abstract::We extend the Saffman theory of membrane hydrodynamics to account for the correlated motion of membrane proteins, along with the effect of protein concentration on that correlation and on the response of the membrane to stresses. Expressions for the coupling diffusion coefficients of protein pairs and their concentrat...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.01.020
更新日期:2009-04-22 00:00:00
abstract::We have identified two kinetically distinct modes of block, by lidocaine, of cardiac sodium channels, activated by batrachotoxin and incorporated into planar lipid bilayers. Here, we analyze the slow blocking mode which appears as a series of nonconducting events that increase in frequency and duration with increasing...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(93)81043-9
更新日期:1993-07-01 00:00:00
abstract::The skeletal muscles of chickens, frogs, and fish have been reported to express two isoforms (alpha and beta) of the sarcoplasmic reticulum calcium release channel (ryanodine receptor or RYR), while mammals express only one. We have studied patterns of RYR isoform expression in skeletal muscles from a variety of fish,...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(93)81303-1
更新日期:1993-12-01 00:00:00
abstract::The continuum and single jump treatments of ion transport through black lipid membranes predict experimentally distinguishable results, even when the same mechanistic assumptions are made and the same potential-distance profile is used. On the basis of steady-state current-voltage curves for nonactin-mediated transpor...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(85)83766-8
更新日期:1985-07-01 00:00:00
abstract::Two-dimensional 1H NMR spectroscopy over a range of temperature through thermal unfolding has been applied to the low-spin, ferric cyanide complex of myoglobin from Aplysia limacina to search for intermediates in the unfolding and to characterize the effect of temperature on the magnetic properties and electronic stru...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.105.062398
更新日期:2005-12-01 00:00:00
abstract::A dimeric alamethicin analog with lysine at position 18 in the sequence (alm-K18) was previously shown to form stable anion-selective channels in membranes at pH 7.0 [Starostin, A. V., R. Butan, V. Borisenko, D. A. James, H. Wenschuh, M. S. Sansom, and G. A. Woolley. 1999. Biochemistry. 38:6144-6150]. To probe the cha...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/s0006-3495(00)76688-4
更新日期:2000-03-01 00:00:00