The barrel-stave model as applied to alamethicin and its analogs reevaluated.

abstract：:Recent experiments suggested that cholesterol and other lipid components of high negative spontaneous curvature facilitate membrane fusion. This is taken as evidence supporting the stalk-pore model of membrane fusion in which the lipid bilayers go through intermediate structures of high curvature. How do the high-curv...

journal_title：Biophysical journal

authors： Wang W,Yang L,Huang HW

更新日期：2007-04-15 00:00:00

abstract：:Diffusion of the chloride salts of Li, Na, K, Rb, and Cs from water into 1-butanol and from 1-butanol into water was examined at temperatures from 13-40 degrees C. Distribution coefficients, interfacial transfer coefficients, Arrhenius activation energy, free energy of activation, enthalpy, and entropy of activation w...

journal_title：Biophysical journal

authors： Ting HP,Bertrand GL,Sears DF

更新日期：1966-11-01 00:00:00

abstract：:The oxygen concentration in tissue can vary on several length scales. The basic scale of variation is determined by capillary spacing. It is this scale that is manifest in the simplest Krogh cylinder model. A second, smaller scale of variation is associated with the consumption of oxygen by mitochondria. This paper gi...

journal_title：Biophysical journal

authors： Clark A Jr,Clark PA

更新日期：1985-12-01 00:00:00

abstract：:The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside living Bacillu...

journal_title：Biophysical journal

authors： Olshausen PV,Defeu Soufo HJ,Wicker K,Heintzmann R,Graumann PL,Rohrbach A

更新日期：2013-09-03 00:00:00

abstract：:A block normal mode (BNM) algorithm, originally proposed by Tama et al., (Proteins Struct. Func. Genet. 41:1-7, 2000) was implemented into the simulation program CHARMM. The BNM approach projects the hessian matrix into local translation/rotation basis vectors and, therefore, dramatically reduces the size of the matri...

journal_title：Biophysical journal

authors： Li G,Cui Q

更新日期：2002-11-01 00:00:00

abstract：:Cell proliferation and contact inhibition play a major role in maintaining epithelial cell homeostasis. Prior experiments have shown that externally applied forces, such as stretch, result in increased proliferation in an E-cadherin force-dependent manner. In this study, the spatial regulation of cell proliferation in...

journal_title：Biophysical journal

authors： Mohan A,Schlue KT,Kniffin AF,Mayer CR,Duke AA,Narayanan V,Arsenovic PT,Bathula K,Danielsson BE,Dumbali SP,Maruthamuthu V,Conway DE

更新日期：2018-09-04 00:00:00

abstract：:Atherosclerosis is a disease initiated by lipoprotein aggregation and deposition in artery walls. In this study, the de novo low-density lipoprotein aggregation process was examined. Nine major intermediates were identified in two stages of the aggregation process. In the aggregation stage, low-density lipoprotein mol...

journal_title：Biophysical journal

authors： Xu S,Lin B

更新日期：2001-10-01 00:00:00

abstract：:The opening of basepairs plays a key role in DNA replication and transcription, and in the action of DNA repair and modification enzymes. In this article, we have used proton exchange to define the energetics of the pathways for basepair opening in two DNA 17-mer duplexes. The rates of exchange of imino protons with s...

journal_title：Biophysical journal

authors： Coman D,Russu IM

更新日期：2005-11-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) is a sensitive and widely used technique for measuring diffusion. FCS data are conventionally modeled with a finite number of diffusing components and fit with a least-square fitting algorithm. This approach is inadequate for analyzing data obtained from highly heterogeneous...

journal_title：Biophysical journal

authors： Sengupta P,Garai K,Balaji J,Periasamy N,Maiti S

更新日期：2003-03-01 00:00:00

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:The field of computational biology has been revolutionized by recent advances in genomics. The completion of a number of genome projects, including that of the human genome, has paved the way toward a variety of challenges and opportunities in bioinformatics and biological systems engineering. One of the first challen...

journal_title：Biophysical journal

authors： Klepeis JL,Floudas CA

更新日期：2003-10-01 00:00:00

abstract：:Investigating the behavior of breast cancer cells via reaction kinetics may help unravel the mechanisms that underlie metabolic changes in tumors. However, obtaining human in vivo kinetic data is challenging because of difficulties associated with measuring these parameters. Nondestructive methods of measuring lipid c...

journal_title：Biophysical journal

authors： Hou J,Reid NE,Tromberg BJ,Potma EO

更新日期：2020-07-21 00:00:00

abstract：:We investigate the validity of continuum electrostatics in the gramicidin A channel using a recently determined high-resolution structure. The potential and electric field acting on ions in and around the channel are computed by solving Poisson's equation. These are then used in Brownian dynamics simulations to obtain...

journal_title：Biophysical journal

authors： Edwards S,Corry B,Kuyucak S,Chung SH

更新日期：2002-09-01 00:00:00

abstract：:Several hundred solid state NMR dipolar couplings and chemical shift anisotropies were simulated for the polytopic membrane protein, bacteriorhodopsin, and for an idealized transmembrane peptide conforming to several different secondary structures (alpha- and 3(10)-helices and parallel and antiparallel beta-sheets), e...

journal_title：Biophysical journal

authors： Sanders CR 2nd,Schwonek JP

更新日期：1993-10-01 00:00:00

abstract：:The fundamental backbone dynamics of unfolded proteins arising due to intrinsic ϕ-ψ dihedral angle fluctuations dictate the course of protein folding, binding, assembly, and function. These internal fluctuations are also critical for protein misfolding associated with a range of human diseases. However, direct observa...

journal_title：Biophysical journal

authors： Jain N,Narang D,Bhasne K,Dalal V,Arya S,Bhattacharya M,Mukhopadhyay S

更新日期：2016-08-23 00:00:00

abstract：:Biological membranes are constantly exposed to forces. The stress-strain relation in membranes determines the behavior of many integral membrane proteins or other membrane related-proteins that show a mechanosensitive behavior. Here, we studied by force spectroscopy the behavior of supported lipid bilayers (SLBs) subj...

journal_title：Biophysical journal

authors： Alessandrini A,Seeger HM,Caramaschi T,Facci P

更新日期：2012-07-03 00:00:00

abstract：:Understanding how polypeptides can efficiently and reproducibly attain a self-entangled conformation is a compelling biophysical challenge that might shed new light on our general knowledge of protein folding. Complex lassos, namely self-entangled protein structures characterized by a covalent loop sealed by a cystein...

journal_title：Biophysical journal

authors： Perego C,Potestio R

更新日期：2019-07-23 00:00:00

abstract：:Proteins that cross-link actin filaments can either form bundles of parallel filaments or isotropic networks of individual filaments. We have found that mixtures of actin filaments with alpha-actinin purified from either Acanthamoeba castellanii or chicken smooth muscle can form bundles or isotropic networks depending...

journal_title：Biophysical journal

authors： Wachsstock DH,Schwartz WH,Pollard TD

更新日期：1993-07-01 00:00:00

abstract：:The triplet state absorption and phosphorescence of Zn and Pd derivatives of myoglobin were compared. Both metal derivatives exhibit long triplet state lifetimes at room temperature, but whereas the Pd derivative showed exponential decay and an isosbestic point in the transient absorption spectra, the decay of the Zn ...

journal_title：Biophysical journal

authors： Papp S,Vanderkooi JM,Owen CS,Holtom GR,Phillips CM

更新日期：1990-07-01 00:00:00

abstract：:A novel system for measuring, simultaneously, ciliary beating and intracellular free calcium is presented. The advantages and dynamic nature of the system are demonstrated by measuring the effects of the calcium ionophore lonomycin and of extracellular ATP on ciliated rabbit trachea. The results are discussed with reg...

journal_title：Biophysical journal

authors： Korngreen A,Priel Z

更新日期：1994-07-01 00:00:00

abstract：:In many situations, cell-cell adhesion is mediated by multiple ligand-receptor pairs. For example, the interaction between T cells and antigen-presenting cells of the immune system is mediated not only by T cell receptors and their ligands (peptide-major histocompatibility complex) but also by binding of intracellular...

journal_title：Biophysical journal

authors： Coombs D,Dembo M,Wofsy C,Goldstein B

更新日期：2004-03-01 00:00:00

abstract：:The primary drivers of yeast endocytosis are actin polymerization and curvature-generating proteins, such as clathrin and BAR domain proteins. Previous work has indicated that these factors may not be capable of generating the forces necessary to overcome turgor pressure. Thus local reduction of the turgor pressure, v...

journal_title：Biophysical journal

authors： Scher-Zagier JK,Carlsson AE

更新日期：2016-12-20 00:00:00

abstract：:The solubility of aqueous solutions of lysozyme in the presence of polyethylene glycol and various alkaline salts was studied experimentally. The protein-electrolyte mixture was titrated with polyethylene glycol, and when precipitation of the protein occurred, a strong increase of the absorbance at 340 nm was observed...

journal_title：Biophysical journal

authors： Boncina M,Rescic J,Vlachy V

更新日期：2008-08-01 00:00:00

abstract：:The properties of vesicle membranes prepared from 16:0-SM, 16:0-DHSM, or DPPC were characterized using steady-state and time-resolved fluorescence spectroscopy and different fluorescent reporter molecules. The acyl-chain region was probed using free and phospholipid-bound 1,6-diphenyl-1,3,5-hexatriene. 16:0-DHSM was f...

journal_title：Biophysical journal

authors： Nyholm T,Nylund M,Söderholm A,Slotte JP

更新日期：2003-02-01 00:00:00

abstract：:Total internal reflection excitation used in combination with fluorescence correlation spectroscopy (TIR-FCS) is a method for characterizing the dynamic behavior and absolute concentrations of fluorescent molecules near or at the interface of a planar substrate and a solution. In this work, we demonstrate for the firs...

journal_title：Biophysical journal

authors： Lieto AM,Cush RC,Thompson NL

更新日期：2003-11-01 00:00:00

abstract：:Five highly homologous epidermal growth factor receptor ligands were studied by mass spectral analysis, hydrogen/deuterium (H/D) exchange via attenuated total reflectance Fourier transform-infrared spectroscopy, and two-dimensional correlation analysis. These studies were performed to determine the order of events dur...

journal_title：Biophysical journal

authors： Iloro I,Narváez D,Guillén N,Camacho CM,Guillén L,Cora E,Pastrana-Ríos B

更新日期：2008-05-15 00:00:00

abstract：:Exchangeable apolipoproteins are located in the surface of lipoprotein particles and regulate lipid metabolism through direct protein-protein and protein-lipid interactions. These proteins are characterized by the presence of tandem repeats of amphiphatic alpha-helix segments and a high surface activity in monolayers ...

journal_title：Biophysical journal

authors： Bolanos-Garcia VM,Renault A,Beaufils S

更新日期：2008-03-01 00:00:00

abstract：:An ab initio model for gene prediction in prokaryotic genomes is proposed based on physicochemical characteristics of codons calculated from molecular dynamics (MD) simulations. The model requires a specification of three calculated quantities for each codon: the double-helical trinucleotide base pairing energy, the b...

journal_title：Biophysical journal

authors： Singhal P,Jayaram B,Dixit SB,Beveridge DL

更新日期：2008-06-01 00:00:00

abstract：:To probe the complexity of the cell membrane organization and dynamics, it is important to obtain simple physical observables from experiments on live cells. Here we show that fluorescence correlation spectroscopy (FCS) measurements at different spatial scales enable distinguishing between different submicron confinem...

journal_title：Biophysical journal

authors： Wawrezinieck L,Rigneault H,Marguet D,Lenne PF

更新日期：2005-12-01 00:00:00

abstract：:We expressed rod-type homotetrameric cyclic nucleotide-gated (CNGA1) channels in Xenopus oocytes and studied activation by photolysis-induced jumps of the 3',5'-cyclic guanosine monophosphate (cGMP) concentration and by voltage steps. cGMP jumps to increasing concentrations up to the EC50 value of 46.5 microM decelera...

journal_title：Biophysical journal

authors： Nache V,Kusch J,Hagen V,Benndorf K

更新日期：2006-05-01 00:00:00