NMR studies of electrostatic potential distribution around biologically important molecules.

abstract：:Fluorescence contributions from immobile sources present a challenge for fluorescence fluctuation spectroscopy (FFS) because the absence of signal fluctuations from stationary fluorophores leads to a biased analysis. This is especially of concern for cellular FFS studies on proteins that interact with immobile structu...

journal_title：Biophysical journal

authors： Skinner JP,Chen Y,Müller JD

更新日期：2008-03-15 00:00:00

abstract：:Label-free imaging techniques such as differential interference contrast (DIC) allow the observation of cells and large subcellular structures in their native, unperturbed states with minimal exposure to light. The development of robust computational image-analysis routines is vital to quantitative label-free imaging....

journal_title：Biophysical journal

authors： Chaphalkar AR,Jawale YK,Khatri D,Athale CA

更新日期：2021-01-11 00:00:00

abstract：:Using a recently developed in vitro motility assay, we have demonstrated that local anesthetics directly inhibit myosin-based movement of single actin filaments in a reversible dose-dependent manner. This is the first reported account of the actions of local anesthetics on purified proteins at the molecular level. In ...

journal_title：Biophysical journal

authors： Tsuda Y,Mashimo T,Yoshiya I,Kaseda K,Harada Y,Yanagida T

更新日期：1996-11-01 00:00:00

abstract：:In many situations, cell-cell adhesion is mediated by multiple ligand-receptor pairs. For example, the interaction between T cells and antigen-presenting cells of the immune system is mediated not only by T cell receptors and their ligands (peptide-major histocompatibility complex) but also by binding of intracellular...

journal_title：Biophysical journal

authors： Coombs D,Dembo M,Wofsy C,Goldstein B

更新日期：2004-03-01 00:00:00

abstract：:A computational framework is presented for studying the mechanical response of macromolecules. The method combines a continuum mechanics (CM) model for the mechanical properties of the macromolecule with a continuum electrostatic (CE) treatment of solvation. The molecules are represented by their shape and key physico...

journal_title：Biophysical journal

authors： Ma L,Yethiraj A,Chen X,Cui Q

更新日期：2009-05-06 00:00:00

abstract：:The possibility that the chlorophyll (chl) ring distortions observed in the crystal structures of chl-protein complexes are involved in the transition energy modulation, giving rise to the spectral forms, is investigated. The out-of-plane chl-macrocycle distortions are described using an orthonormal set of deformation...

journal_title：Biophysical journal

authors： Zucchelli G,Brogioli D,Casazza AP,Garlaschi FM,Jennings RC

更新日期：2007-09-15 00:00:00

abstract：:The relative contributions of the allosteric and affinity factors toward the change in p50 have been calculated for a series of effectors of hemoglobin (Hb). Shifts in the ligand affinity of deoxy Hb and the values for 50% ligand saturation (p50) were obtained from oxygen equilibrium data. Because the high-affinity pa...

journal_title：Biophysical journal

authors： Marden MC,Bohn B,Kister J,Poyart C

更新日期：1990-03-01 00:00:00

abstract：:Proton translocation activity of bacteriorhodopsin mutants lacking the proton acceptor Asp-85 was investigated using the black lipid membrane technique. Mutants D85N, D85T, and D85,96N were constructed and homologously expressed in Halobacterium salinarium to yield a membrane fraction with a buoyant density of 1.18 g/...

journal_title：Biophysical journal

authors： Tittor J,Schweiger U,Oesterhelt D,Bamberg E

更新日期：1994-10-01 00:00:00

abstract：:Voltage-sensitive fluorescent dyes are commonly used to measure cardiac electrical activity. Recent studies indicate, however, that optical action potentials (OAPs) recorded from the myocardial surface originate from a widely distributed volume beneath the surface and may contain useful information regarding intramura...

journal_title：Biophysical journal

authors： Hyatt CJ,Mironov SF,Wellner M,Berenfeld O,Popp AK,Weitz DA,Jalife J,Pertsov AM

更新日期：2003-10-01 00:00:00

abstract：:A detailed analysis is made of the model system of two parallel cylindrical polyelectrolytes which contain ionizable groups on their surfaces and are immersed in an ionic bathing medium. The interaction between the cylinders is examined by considering the interplay between repulsive electrostatic forces and attractive...

journal_title：Biophysical journal

authors： Brenner SL,McQuarrie DA

更新日期：1973-04-01 00:00:00

abstract：:Measurements of transepithelial electrical impedance of continuously short-circuited A6 epithelia were made at audio frequencies (0.244 Hz to 10.45 kHz) to investigate the time course and extent to which prostaglandin E(2) (PGE(2)) modulates Cl(-) transport and apical membrane capacitance in this cell-cultured model e...

journal_title：Biophysical journal

authors： Păunescu TG,Helman SI

更新日期：2001-08-01 00:00:00

abstract：:The energy transfer processes between carotenoids and Chls have been studied by femtosecond transient absorption in the CP29-WT complex, which contains only two carotenoids per polypeptide located in the L1 and L2 sites, and in the CP29-E166V mutant in which only the L1 site is occupied. The comparison of these two sa...

journal_title：Biophysical journal

authors： Croce R,Müller MG,Caffarri S,Bassi R,Holzwarth AR

更新日期：2003-04-01 00:00:00

abstract：:Control of organelle abundance is a fundamental unsolved problem in cell biology. Mechanisms for number control have been proposed in which organelle assembly is actively increased or decreased to compensate for deviations from a set-point, but such phenomena have not been experimentally verified. In this report we ex...

journal_title：Biophysical journal

authors： Marshall WF

更新日期：2007-09-01 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:Using both analytical solutions obtained from simplified systems and numerical results from more realistic cases, we investigate the role played by the dielectric constant of membrane proteins epsilon(p) and pore water epsilon(w) in permeation of ions across channels. We show that the boundary and its curvature are th...

journal_title：Biophysical journal

authors： Baştuğ T,Kuyucak S

更新日期：2003-05-01 00:00:00

abstract：:Molecular dynamics simulations of RNA-protein complex between Escherichia coli loop E/helix IV (LE/HeIV) rRNA and L25 protein reveal a qualitative agreement between the experimental and simulated structures. The major groove of LE is a prominent rRNA cation-binding site. Divalent cations rigidify the LE major groove g...

journal_title：Biophysical journal

authors： Réblová K,Spacková N,Koca J,Leontis NB,Sponer J

更新日期：2004-11-01 00:00:00

abstract：:Sedimentation velocity is a powerful tool for the analysis of complex solutions of macromolecules. However, sample turbidity imposes an upper limit to the size of molecular complexes currently amenable to such analysis. Furthermore, the breadth of the particle size distribution, combined with possible variations in th...

journal_title：Biophysical journal

authors： Mächtle W

更新日期：1999-02-01 00:00:00

abstract：:At low surface concentrations that permit formation of impermeable membranes, neuronal soluble N-ethyl maleimide sensitive factor attachment protein receptor (SNARE) proteins form a stable, parallel, trans complex when vesicles are brought into contact by a low concentration of poly(ethylene glycol) (PEG). Surprisingl...

journal_title：Biophysical journal

authors： Dennison SM,Bowen ME,Brunger AT,Lentz BR

更新日期：2006-03-01 00:00:00

abstract：:Resolving the dynamical interplay of proteins and lipids in the live-cell plasma membrane represents a central goal in current cell biology. Superresolution concepts have introduced a means of capturing spatial heterogeneity at a nanoscopic length scale. Similar concepts for detecting dynamical transitions (superresol...

journal_title：Biophysical journal

authors： Ruprecht V,Wieser S,Marguet D,Schütz GJ

更新日期：2011-06-08 00:00:00

abstract：:PIEZO1 is an inactivating eukaryotic cation-selective mechanosensitive ion channel. Two sites have been located in the channel that when individually mutated lead to xerocytotic anemia by slowing inactivation. By introducing mutations at two sites, one associated with xerocytosis and the other artificial, we were able...

journal_title：Biophysical journal

authors： Bae C,Gottlieb PA,Sachs F

更新日期：2013-08-20 00:00:00

abstract：:The recently developed method of site-directed Fourier transform infrared dichroism for obtaining orientational constraints of oriented polymers is applied here to the transmembrane domain of the vpu protein from the human immunodeficiency virus type 1 (HIV-1). The infrared spectra of the 31-residue-long vpu peptide r...

journal_title：Biophysical journal

authors： Kukol A,Arkin IT

更新日期：1999-09-01 00:00:00

abstract：:Under the assumption that vesicles are the anatomic correlate of quantal release, the forces governing the movement of synaptic vesicles inside neurons are analyzed. Semiquantitative calculations are presented to show that a diffuse layer field penetrates a few Debye lengths into the axoplasm. This field binds tightly...

journal_title：Biophysical journal

authors： Remler MP

更新日期：1973-02-01 00:00:00

abstract：:Evidence that the fluorophore sempervirene binds to nucleic acids is presented. The complexes were studied by fluorescence intensity, spectra, decay lifetime, and polarization methods. Both fluorescent and nonfluorescent complexes are formed. The sempervirene is rigidly fixed to DNA. If ethidium bromide and sempervire...

journal_title：Biophysical journal

authors： Burns VW

更新日期：1974-03-01 00:00:00

abstract：:Dielectrophoresis and electrorotation are commonly used to measure dielectric properties and membrane electrical parameters of biological cells. We have derived quantitative relationships for several critical points, defined in Fig. A 1, which characterize the dielectrophoretic spectrum and the electrorotational spect...

journal_title：Biophysical journal

authors： Gimsa J,Marszalek P,Loewe U,Tsong TY

更新日期：1991-10-01 00:00:00

abstract：:Many multicellular communities propagate signals in a directed manner via excitable waves. Cell-to-cell heterogeneity is a ubiquitous feature of multicellular communities, but the effects of heterogeneity on wave propagation are still unclear. Here, we use a minimal FitzHugh-Nagumo-type model to investigate excitable ...

journal_title：Biophysical journal

authors： Zhai X,Larkin JW,Süel GM,Mugler A

更新日期：2020-04-07 00:00:00

abstract：:In some fungi, a premeiotic process known as repeat-induced point mutation (RIP) can accurately identify and mutate nearly all gene-sized DNA repeats present in the haploid germline nuclei. Studies in Neurospora crassa have suggested that RIP detects sequence homology directly between intact DNA double helices, withou...

journal_title：Biophysical journal

authors： Mazur AK,Gladyshev E

更新日期：2018-08-21 00:00:00

abstract：:Ionotropic glutamate receptors are essential for fast synaptic nerve transmission. Recent x-ray structures for the ligand-binding (S1S2) region of the GluR2 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-sensitive receptor have suggested how differences in protein/ligand interactions may determine wh...

journal_title：Biophysical journal

authors： Arinaminpathy Y,Sansom MS,Biggin PC

更新日期：2002-02-01 00:00:00

abstract：:The distribution of a selection of cationic fluorescent dyes can be used to measure the membrane potential of individual cells with a microfluorometer. The essential attributes of these dyes include membrane permeability, low membrane binding, spectral properties which are insensitive to environment, and, of course, s...

journal_title：Biophysical journal

authors： Ehrenberg B,Montana V,Wei MD,Wuskell JP,Loew LM

更新日期：1988-05-01 00:00:00

abstract：:Cardiac transverse (T)-tubules provide a specialized structure for synchronization and stabilization of sarcoplasmic reticulum Ca(2+) release in healthy cardiomyocytes. The application of laser scanning confocal microscopy and the use of fluorescent lipophilic membrane dyes have boosted the discoveries that T-tubule r...

journal_title：Biophysical journal

authors： Guo A,Song LS

更新日期：2014-06-17 00:00:00

abstract：:Experimental approaches such as fiber diffraction and cryo-electron microscopy reconstruction have defined regulatory positions of tropomyosin on actin but have not, as yet, succeeded at determining key atomic-level contacts between these proteins or fully substantiated the dynamics of their interactions at a structur...

journal_title：Biophysical journal

authors： Pavadai E,Lehman W,Rynkiewicz MJ

更新日期：2020-07-07 00:00:00