Studies on the fluorophore sempervirene and its complexes with DNA.

abstract：:A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found for the various parts of the life cycle: S, 4.13 hours; G1, 4.71 hours; G2, 2.81 hours; mitosis, 0.81 hours. The cell has a to...

journal_title：Biophysical journal

authors： PUCK TT,SANDERS P,PETERSEN D

更新日期：1964-11-01 00:00:00

abstract：:We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system and derive a general expression for the fluorescence autocorrelation function that applies w...

journal_title：Biophysical journal

authors： McHale K,Mabuchi H

更新日期：2010-07-07 00:00:00

abstract：:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Prot...

journal_title：Biophysical journal

authors： van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

更新日期：2013-03-05 00:00:00

abstract：:Coarse-grained (CG) models of biomolecules have recently attracted considerable interest because they enable the simulation of complex biological systems on length-scales and timescales that are inaccessible for atomistic molecular dynamics simulation. A CG model is defined by a map that transforms an atomically detai...

journal_title：Biophysical journal

authors： Zhang Z,Lu L,Noid WG,Krishna V,Pfaendtner J,Voth GA

更新日期：2008-12-01 00:00:00

abstract：:We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displa...

journal_title：Biophysical journal

authors： Terpetschnig E,Szmacinski H,Malak H,Lakowicz JR

更新日期：1995-01-01 00:00:00

abstract：:The structural dynamics of bovine erythrocyte Cu, Zn superoxide dismutase (BSOD) was studied by time-resolved fluorescence spectroscopy. BSOD is a homodimer containing a single tyrosine residue (and no tryptophan) per subunit. Frequency-domain fluorometry revealed a heterogeneous fluorescence decay that could be descr...

journal_title：Biophysical journal

authors： Ferreira ST,Stella L,Gratton E

更新日期：1994-04-01 00:00:00

abstract：:The regulatory mechanism of sarcomeric activity has not been fully clarified yet because of its complex and cooperative nature, which involves both Ca(2+) and cross-bridge binding to the thin filament. To reveal the mechanism of regulation mediated by the cross-bridges, separately from the effect of Ca(2+), we investi...

journal_title：Biophysical journal

authors： Shimamoto Y,Kono F,Suzuki M,Ishiwata S

更新日期：2007-12-15 00:00:00

abstract：:Fusion is a crucial event in the infection of animal cells by enveloped viruses (e.g., HIV or influenza). Viral fusion is mediated by glycoproteins, spanning the viral envelope, which attach to a membrane surface and induce fusion of the viral envelope to the cellular membrane. Influenza fusion protein (hemagglutinin)...

journal_title：Biophysical journal

authors： Longo ML,Waring AJ,Hammer DA

更新日期：1997-09-01 00:00:00

abstract：:An analysis of four 1-ns molecular dynamics trajectories for two different 15-bp oligonucleotides is presented. Our aim is to show which groups of atoms can be treated as rigid bodies within a bead representation of DNA, independently of the base sequence and for any conformations belonging to the A/B family. Five mod...

journal_title：Biophysical journal

authors： Bruant N,Flatters D,Lavery R,Genest D

更新日期：1999-11-01 00:00:00

abstract：:We are interested in modeling the membrane-spanning domain of the serotonin 5-HT1A G-protein coupled receptor. This superfamily of proteins is predicted to share the topology of the seven transmembrane helices of bacteriorhodopsin (BR), even though no significant sequence homology had been identified. We found signifi...

journal_title：Biophysical journal

authors： Ballesteros JA,Weinstein H

更新日期：1992-04-01 00:00:00

abstract：:Sphingomyelin is an abundant lipid in some cellular membrane domains, such as lipid rafts. Hydrogen bonding and hydrophobic interactions of the lipid with surrounding components such as neighboring sphingomyelin and cholesterol (Cho) are widely considered to stabilize the raft-like liquid-ordered (Lo) domains in membr...

journal_title：Biophysical journal

authors： Yano Y,Hanashima S,Yasuda T,Tsuchikawa H,Matsumori N,Kinoshita M,Al Sazzad MA,Slotte JP,Murata M

更新日期：2018-10-16 00:00:00

abstract：:Calcium is essential for many biological processes involved in cellular motility. However, the pathway by which calcium influences motility, in processes such as muscle contraction and neuronal growth, is often indirect and complex. We establish a simple and direct mechanochemical link that shows how calcium quantitat...

journal_title：Biophysical journal

authors： Tam BK,Shin JH,Pfeiffer E,Matsudaira P,Mahadevan L

更新日期：2009-08-19 00:00:00

abstract：:The effect of Ca ion concentration on cross-bridge kinetics in a small bundle (one to three fibers) of chemically skinned rabbit psoas muscle is studied. The length of the muscle is oscillated in small amplitude sine waves (0.2% L0 peak-to-peak) at varying frequencies (0.125 -- 167 Hz), and the resulting amplitude and...

journal_title：Biophysical journal

authors： Kawai M,Cox RN,Brandt PW

更新日期：1981-08-01 00:00:00

abstract：:The switch 1 region of myosin forms a lid over the nucleotide phosphates as part of a structure known as the phosphate-tube. The homologous region in kinesin-family motors is more open, not interacting with the nucleotide. We used molecular dynamics (MD) simulations to examine a possible displacement of switch 1 of th...

journal_title：Biophysical journal

authors： Minehardt TJ,Cooke R,Pate E,Kollman PA

更新日期：2001-03-01 00:00:00

abstract：:Conventional equations for enzyme kinetics are based on mass-action laws, that may fail in low-dimensional and disordered media such as biological membranes. We present Monte Carlo simulations of an isolated Michaelis-Menten enzyme reaction on two-dimensional lattices with varying obstacle densities, as models of biol...

journal_title：Biophysical journal

authors： Berry H

更新日期：2002-10-01 00:00:00

abstract：:In contrast with most inhalational anesthetics, the anesthetic gases xenon (Xe) and nitrous oxide (N(2)O) act by blocking the N-methyl-d-aspartate (NMDA) receptor. Using x-ray crystallography, we examined the binding characteristics of these two gases on two soluble proteins as structural models: urate oxidase, which ...

journal_title：Biophysical journal

authors： Colloc'h N,Sopkova-de Oliveira Santos J,Retailleau P,Vivarès D,Bonneté F,Langlois d'Estainto B,Gallois B,Brisson A,Risso JJ,Lemaire M,Prangé T,Abraini JH

更新日期：2007-01-01 00:00:00

abstract：:Conductance noise measurement of the open states of alamethicin transmembrane channels reveals excess noise attributable to cooperative low-frequency molecular dynamics that can generate fluctuations approximately 1 A rms in the effective channel pore radius. Single-channel currents through both persistent and nonpers...

journal_title：Biophysical journal

authors： Mak DO,Webb WW

更新日期：1995-12-01 00:00:00

abstract：:Nonconfluent fibroblasts are relatively depolarized when compared with confluent fibroblasts, and transient hyperpolarizations result from a range of external stimuli as well as internal cellular activities. This electrical activity ceases, along with growth and mitogenic activity, when the cells become confluent. A c...

journal_title：Biophysical journal

authors： Stockbridge LL,French AS

更新日期：1988-07-01 00:00:00

abstract：:We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described met...

journal_title：Biophysical journal

authors： Abreu MS,Moreno MJ,Vaz WL

更新日期：2004-07-01 00:00:00

abstract：:Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Amon...

journal_title：Biophysical journal

authors： Capitanio M,Pavone FS

更新日期：2013-09-17 00:00:00

abstract：:A combined experimental and theoretical study is performed on binary dilauroylphosphatidylcholine/distearoylphosphatidylcholine (DLPC/DSPC) lipid bilayer membranes incorporating bacteriorhodopsin (BR). The system is designed to investigate the possibility that BR, via a hydrophobic matching principle related to the di...

journal_title：Biophysical journal

authors： Dumas F,Sperotto MM,Lebrun MC,Tocanne JF,Mouritsen OG

更新日期：1997-10-01 00:00:00

abstract：:Polarized photolysis experiments have been performed on the carbon monoxide complex of myoglobin to assess the effects of photoselection on the kinetics of ligand rebinding and to investigate the reorientational dynamics of the heme plane. The results are analyzed in terms of the optical theory developed in the preced...

journal_title：Biophysical journal

authors： Ansari A,Jones CM,Henry ER,Hofrichter J,Eaton WA

更新日期：1993-03-01 00:00:00

abstract：:The geometry of domains in phospholipid bilayers of binary (1:1) mixtures of synthetic lecithins with a difference in chain length of four methylene groups has been studied by two independent, direct and complementary methods. Grazing incidence diffraction of neutrons provided gel domain sizes of less than 10 nm in bo...

journal_title：Biophysical journal

authors： Gliss C,Clausen-Schaumann H,Günther R,Odenbach S,Randl O,Bayerl TM

更新日期：1998-05-01 00:00:00

abstract：:The conduction properties of ClC-0 and ClC-1 chloride channels are examined using electrostatic calculations and three-dimensional Brownian dynamics simulations. We create an open-state configuration of the prokaryotic ClC Cl(-) channel using its known crystallographic structure as a basis. Two residues that are occlu...

journal_title：Biophysical journal

authors： Corry B,O'Mara M,Chung SH

更新日期：2004-02-01 00:00:00

abstract：:In maximally activated skinned fibers, the rate of tension redevelopment (ktr) following a rapid release and restretch is determined by the maximal rate of cross-bridge cycling. During submaximal Ca2+ activations, however, ktr regulation varies with thin filament dynamics. Thus, decreasing the rate of Ca2+ dissociatio...

journal_title：Biophysical journal

authors： Regnier M,Rivera AJ,Chase PB,Smillie LB,Sorenson MM

更新日期：1999-05-01 00:00:00

abstract：:We present a new method for analyzing the dynamics of conformational fluctuations of individual flexible polymer molecules. In single-particle tracking (SPT), one end of the polymer molecule is tethered to an immobile substratum. A microsphere attached to the other end serves as an optical marker. The conformational f...

journal_title：Biophysical journal

authors： Qian H,Elson EL

更新日期：1999-03-01 00:00:00

abstract：:The stoichiometric matrix, S, represents a mapping of reaction rate vectors into a space of concentration time derivatives. The left null space of the stoichiometric matrix contains the dynamic invariants: a combination of concentration variables, referred to as metabolic pools, whose total concentration does not chan...

journal_title：Biophysical journal

authors： Famili I,Palsson BO

更新日期：2003-07-01 00:00:00

abstract：:The closed-state crystal structure of prokaryotic inward rectifier, KirBac1.1, has implicated four inner helical phenylalanines near the cytoplasmic side as a possible locus of the channel gate. In the present study, we investigate whether this structural feature corresponds to the physiological pH gate of the renal i...

journal_title：Biophysical journal

authors： Sackin H,Nanazashvili M,Palmer LG,Krambis M,Walters DE

更新日期：2005-04-01 00:00:00

abstract：:Morphogenesis of epithelial tissues requires tight spatiotemporal coordination of cell shape changes. In vivo, many tissue-scale shape changes are driven by pulsatile contractions of intercellular junctions, which are rectified to produce irreversible deformations. The functional role of this pulsatory ratchet and its...

journal_title：Biophysical journal

authors： Staddon MF,Cavanaugh KE,Munro EM,Gardel ML,Banerjee S

更新日期：2019-11-05 00:00:00

abstract：:The actin cytoskeleton fulfills numerous key cellular functions, which are tightly regulated in activity, localization, and temporal patterning by actin binding proteins. Tropomyosins and gelsolin are two such filament-regulating proteins. Here, we investigate how the effects of tropomyosins are coupled to the binding...

journal_title：Biophysical journal

authors： Kis-Bicskei N,Bécsi B,Erdődi F,Robinson RC,Bugyi B,Huber T,Nyitrai M,Talián GC

更新日期：2018-02-27 00:00:00