Analysis and refinement of criteria for predicting the structure and relative orientations of transmembranal helical domains.

abstract：:Polymers play an important role in many biological systems, so a fundamental understanding of their cross-links is crucial not only for the development of medicines but also for the development of biomimetic materials. The biomechanical movements of all mammals are aided by tendon fibrils. The self-organization and bi...

journal_title：Biophysical journal

authors： Gutsmann T,Hassenkam T,Cutroni JA,Hansma PK

更新日期：2005-07-01 00:00:00

abstract：:The nucleus has a smooth, regular appearance in normal cells, and its shape is greatly altered in human pathologies. Yet, how the cell establishes nuclear shape is not well understood. We imaged the dynamics of nuclear shaping in NIH3T3 fibroblasts. Nuclei translated toward the substratum and began flattening during t...

journal_title：Biophysical journal

authors： Li Y,Lovett D,Zhang Q,Neelam S,Kuchibhotla RA,Zhu R,Gundersen GG,Lele TP,Dickinson RB

更新日期：2015-08-18 00:00:00

abstract：:The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at ...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Hegemann P,Oesterhelt D

更新日期：1985-01-01 00:00:00

abstract：:In this work we have designed a simple system to investigate empirically the image contrast of tapping-mode atomic force microscopy (TMAFM). We modified the cantilever tips with protein molecules (bovine serum albumin or goat anti-biotin antibody) and used these protein-modified cantilevers to scan poly-L-lysine films...

journal_title：Biophysical journal

authors： You H,Yu L

更新日期：1997-12-01 00:00:00

abstract：:The effects of cholesterol (Chol) on phospholipid bilayers include ordering of the fatty acyl chains, condensing of the lipids in the bilayer plane, and promotion of the liquid-ordered phase. These effects depend on the type of phospholipids in the bilayer and are determined by the nature of the underlying molecular i...

journal_title：Biophysical journal

authors： Aittoniemi J,Niemelä PS,Hyvönen MT,Karttunen M,Vattulainen I

更新日期：2007-02-15 00:00:00

abstract：:A discrete multistate kinetic model for water-wire proton transport is constructed and analyzed using Monte Carlo simulations. In the model, each water molecule can be in one of three states: oxygen lone-pairs pointing leftward, pointing rightward, or protonated (H(3)O(+)). Specific rules for transitions among these s...

journal_title：Biophysical journal

authors： Chou T

更新日期：2004-05-01 00:00:00

abstract：:In contrast with most inhalational anesthetics, the anesthetic gases xenon (Xe) and nitrous oxide (N(2)O) act by blocking the N-methyl-d-aspartate (NMDA) receptor. Using x-ray crystallography, we examined the binding characteristics of these two gases on two soluble proteins as structural models: urate oxidase, which ...

journal_title：Biophysical journal

authors： Colloc'h N,Sopkova-de Oliveira Santos J,Retailleau P,Vivarès D,Bonneté F,Langlois d'Estainto B,Gallois B,Brisson A,Risso JJ,Lemaire M,Prangé T,Abraini JH

更新日期：2007-01-01 00:00:00

abstract：:Many proteins are anchored to lipid bilayer membranes through a combination of hydrophobic and electrostatic interactions. In the case of the membrane-bound nonreceptor tyrosine kinase Src from Rous sarcoma virus, these interactions are mediated by an N-terminal myristoyl chain and an adjacent cluster of six basic ami...

journal_title：Biophysical journal

authors： Scheidt HA,Klingler J,Huster D,Keller S

更新日期：2015-08-04 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:Muscle contraction is governed by tropomyosin (Tpm) shifting azimuthally between three states on F-actin (B-, C-, and M-states) in response to calcium binding to troponin and actomyosin cross-bridge formation. The Tpm coiled coil polymerizes head to tail along the long-pitch helix of F-actin to form continuous superhe...

journal_title：Biophysical journal

authors： Sundar S,Rynkiewicz MJ,Ghosh A,Lehman W,Moore JR

更新日期：2020-01-21 00:00:00

abstract：:Measurements were made of exchange rates of oxygen, carbon dioxide, and krypton-85 with blood at 37.5 degrees C. Gas transfer took place across a 1 mil silicone rubber membrane. The blood was in a rotating disk boundary layer flow, and the controlling resistance to transfer was the concentration boundary layer. Measur...

journal_title：Biophysical journal

authors： Spaeth EE,Friedlander SK

更新日期：1967-11-01 00:00:00

abstract：:The classical cable equation, in which membrane conductance is considered constant, is modified by including the linearized effect of membrane potential on sodium and potassium ionic currents, as formulated in the Hodgkin-Huxley equations for the squid giant axon. The resulting partial differential equation is solved ...

journal_title：Biophysical journal

authors： Sabah NH,Leibovic KN

更新日期：1969-10-01 00:00:00

abstract：:The formation and function of the mitotic spindle depends upon force generation by multiple molecular motors and by the dynamics of microtubules, but how these force-generating mechanisms relate to one another is unclear. To address this issue we have modeled the separation of spindle poles as a function of time durin...

journal_title：Biophysical journal

authors： Cytrynbaum EN,Scholey JM,Mogilner A

更新日期：2003-02-01 00:00:00

abstract：:Bistability is a major mechanism for cellular decision making and usually results from positive feedback in biochemical control systems. Here we show theoretically that bistability between unbound and bound states of adhesion clusters results from positive feedback mediated by structural rather than biochemical proces...

journal_title：Biophysical journal

authors： Erdmann T,Schwarz US

更新日期：2006-09-15 00:00:00

abstract：:Phospholipids with covalently attached poly(ethylene glycol) (PEG lipids) are commonly used for the preparation of long circulating liposomes. Although it is well known that lipid/PEG-lipid mixed micelles may form above a certain critical concentration of PEG-lipid, little is known about the effects of PEG-lipids on l...

journal_title：Biophysical journal

authors： Edwards K,Johnsson M,Karlsson G,Silvander M

更新日期：1997-07-01 00:00:00

abstract：:Topical microbicide products are being developed for the prevention of sexually transmitted infections. These include vaginally-applied gels that deliver anti-HIV molecules. Gels may also provide partial barriers that slow virion diffusion from semen to vulnerable epithelium, increasing the time during which anti-HIV ...

journal_title：Biophysical journal

authors： Lai BE,Henderson MH,Peters JJ,Walmer DK,Katz DF

更新日期：2009-11-04 00:00:00

abstract：:The propagation of sounds and pulse waves within the cardiovascular system is subject to strong dissipative mechanisms. To investigate the effects of blood viscosity on dissipation as well as dispersion of small waves in arteries and veins, a parametric study has been carried out. A linearized analysis of axisymmetric...

journal_title：Biophysical journal

authors： Jones E,Anliker M,Chang ID

更新日期：1971-12-01 00:00:00

abstract：:The effects of intracellular magnesium ions and extracellular calcium and magnesium ions on the delayed rectifier potassium ion channel, IK, were investigated from intracellularly perfused squid giant axons. Cao+2 and Mgo+2 both blocked IK in a voltage-independent manner with a KD of approximately 100 and 500 mM, resp...

journal_title：Biophysical journal

authors： Clay JR

更新日期：1995-11-01 00:00:00

abstract：:Ca2+ regulates ryanodine receptor's (RyR) activity through an activating and an inhibiting Ca2+-binding site located on the cytoplasmic side of the RyR channel. Their altered sensitivity plays an important role in the pathology of malignant hyperthermia and heart failure. We used lanthanide ions (Ln3+) as probes to in...

journal_title：Biophysical journal

authors： Sárközi S,Komáromi I,Jóna I,Almássy J

更新日期：2017-05-23 00:00:00

abstract：:Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particles similar to nascent high-density lipoproteins, which reduce the risk of coronary artery disease. The major protein component of high-density lipoproteins is human apolipoprotein A-I, and the corresponding protein component of Nanodis...

journal_title：Biophysical journal

authors： Li Y,Kijac AZ,Sligar SG,Rienstra CM

更新日期：2006-11-15 00:00:00

abstract：:Finite difference solutions of the Poisson-Boltzmann equation are used to calculate the pKa values of the functionally important ionizable groups in bacteriorhodopsin. There are strong charge-charge interactions between the residues in the binding site leading to the possibility of complex titration behavior. Structur...

journal_title：Biophysical journal

authors： Sampogna RV,Honig B

更新日期：1994-05-01 00:00:00

abstract：:Surface charge in track-etched polyethylene terephthalate (PET) membranes with narrow pores has been probed with a fluorescent cationic dye (3,3'-diethyloxacarbocyanine iodide (diO-C2-(3))) using confocal microscopy. Staining of negatively charged PET membranes with diO-C2-(3) is a useful measure of surface charge for...

journal_title：Biophysical journal

authors： Bashford CL,Alder GM,Pasternak CA

更新日期：2002-04-01 00:00:00

abstract：:The possibility that the chlorophyll (chl) ring distortions observed in the crystal structures of chl-protein complexes are involved in the transition energy modulation, giving rise to the spectral forms, is investigated. The out-of-plane chl-macrocycle distortions are described using an orthonormal set of deformation...

journal_title：Biophysical journal

authors： Zucchelli G,Brogioli D,Casazza AP,Garlaschi FM,Jennings RC

更新日期：2007-09-15 00:00:00

abstract：:The persistence length of titin from rabbit skeletal muscles was measured using a combination of static and dynamic light scattering, and neutron small angle scattering. Values of persistence length in the range 9-16 nm were found for titin-II, which corresponds to mainly physiologically inelastic A-band part of the p...

journal_title：Biophysical journal

authors： Di Cola E,Waigh TA,Trinick J,Tskhovrebova L,Houmeida A,Pyckhout-Hintzen W,Dewhurst C

更新日期：2005-06-01 00:00:00

abstract：:The folding pathway of the small α/β protein GB1 has been extensively studied during the past two decades using both theoretical and experimental approaches. These studies provided a consensus view that the protein folds in a two-state manner. Here, we reassessed the folding of GB1, both by experiments and simulations...

journal_title：Biophysical journal

authors： Morrone A,Giri R,Toofanny RD,Travaglini-Allocatelli C,Brunori M,Daggett V,Gianni S

更新日期：2011-10-19 00:00:00

abstract：:Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

journal_title：Biophysical journal

authors： Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

更新日期：2008-06-01 00:00:00

abstract：:Experimental studies have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high repetition rate lasers, a phenomenon we call "light quenching." We now describe the theory of light quenching and some of its effects on the steady-state and time-resolved intensity and ani...

journal_title：Biophysical journal

authors： Kuśba J,Bogdanov V,Gryczynski I,Lakowicz JR

更新日期：1994-11-01 00:00:00

abstract：:The statistical mechanics of cross-bridge action is considered in order to develop constitutive equations that express fiber tension as a function of degree of activation and time history of speed of contraction. The kinetic equation of A.F. Huxley (1) is generalized to apply to the partially activated state. The rate...

journal_title：Biophysical journal

authors： Tözeren A

更新日期：1985-02-01 00:00:00

abstract：:Steady-state polarization-resolved fluorescence imaging is used to analyze the molecular orientational order behavior of rigidly labeled major histocompatibility complex class I (MHC I) proteins and lipid probes in cell membranes of living cells. These fluorescent probes report the orientational properties of proteins...

journal_title：Biophysical journal

authors： Kress A,Ferrand P,Rigneault H,Trombik T,He HT,Marguet D,Brasselet S

更新日期：2011-07-20 00:00:00

abstract：:The effects of chemical modifications of myosin's reactive cysteines on actomyosin adenosine triphosphatase (ATPase) activities and sliding velocities in the in vitro motility assays were examined in this work. The three types of modifications studied were 4-[N-[(iodoacetoxy)ethyl]-N-methylamino]-7-nitrobenz-2-oxa-1,3...

journal_title：Biophysical journal

authors： Root DD,Reisler E

更新日期：1992-09-01 00:00:00