Probing orientational behavior of MHC class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging.

abstract：:EmrE is a small multidrug resistance transporter that has been well studied as a model for secondary active transport. Because transport requires the protein to convert between at least two states open to opposite sides of the membrane, it is expected that blocking these conformational transitions will prevent transpo...

journal_title：Biophysical journal

authors： Dutta S,Morrison EA,Henzler-Wildman KA

更新日期：2014-08-05 00:00:00

abstract：:Aggregated Markov models are a widely used tool to model patch clamp data measured from single ion channels. These channels must obey the principle of detailed balance in thermodynamic equilibrium; otherwise, the channel is driven by an external source of energy. We investigate the power of a likelihood ratio test for...

journal_title：Biophysical journal

authors： Wagner M,Timmer J

更新日期：2000-12-01 00:00:00

abstract：:Antibodies are key tools in biomedical research and medicine. Their binding properties are classically measured in solution and characterized by an affinity. However, in physiological conditions, antibodies can bridge an immune effector cell and an antigen-presenting cell, implying that mechanical forces may apply to ...

journal_title：Biophysical journal

authors： González C,Chames P,Kerfelec B,Baty D,Robert P,Limozin L

更新日期：2019-04-23 00:00:00

abstract：:Molecular dynamics simulations are presented for a Thermus aquaticus (Taq) DNA polymerase I complex (consisting of the protein, the primer-template DNA strands, and the incoming nucleotide) subjected to external forces. The results obtained with a force applied to the DNA template strand provide insights into the effe...

journal_title：Biophysical journal

authors： Andricioaei I,Goel A,Herschbach D,Karplus M

更新日期：2004-09-01 00:00:00

abstract：:DNA release from lipoplexes is an essential step during lipofection and is probably a result of charge neutralization by cellular anionic lipids. As a model system to test this possibility, fluorescence resonance energy transfer between DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to mo...

journal_title：Biophysical journal

authors： Tarahovsky YS,Koynova R,MacDonald RC

更新日期：2004-08-01 00:00:00

abstract：:The lipid matrix present in the uppermost layer of the skin, the stratum corneum, plays a crucial role in the skin barrier function. The lipids are organized into two lamellar phases. To gain more insight into the molecular organization of one of these lamellar phases, we performed neutron diffraction studies. In the ...

journal_title：Biophysical journal

authors： Groen D,Gooris GS,Barlow DJ,Lawrence MJ,van Mechelen JB,Demé B,Bouwstra JA

更新日期：2011-03-16 00:00:00

abstract：:Two different spin labels, N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)iodoacetamide (IPSL) and (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate (MTSSL), and two different fluorescent labels 5-((((2-iodoacetyl)amino)-ethyl)amino)naphtalene-1-sulfonic acid (IAEDANS) and 6-bromoacetyl-2-dimetylaminon...

journal_title：Biophysical journal

authors： Hammarström P,Owenius R,Mårtensson LG,Carlsson U,Lindgren M

更新日期：2001-06-01 00:00:00

abstract：:The rat connexin40 gap junction channel is permeable to monovalent cations including tetramethylammonium and tetraethylammonium ions. Larger tetraalkyammonium (TAA(+)) ions beginning with tetrabutylammonium (TBA(+)) reduced KCl junctional currents disproportionately. Ionic blockade by tetrapentylammonium (TPeA(+)) and...

journal_title：Biophysical journal

authors： Musa H,Gough JD,Lees WJ,Veenstra RD

更新日期：2001-12-01 00:00:00

abstract：:A new solid-state NMR-based strategy is established for the precise and efficient analysis of orientation and dynamics of transmembrane peptides in fluid bilayers. For this purpose, several dynamically averaged anisotropic constraints, including (13)C and (15)N chemical shift anisotropies and (13)C-(15)N dipolar coupl...

journal_title：Biophysical journal

authors： Holt A,Rougier L,Réat V,Jolibois F,Saurel O,Czaplicki J,Killian JA,Milon A

更新日期：2010-05-19 00:00:00

abstract：:Deuterium magnetic resonance is applied to the study of the liquid crystalline and gel phases, and of the phase transition, of a multilamellar dispersion of chain perdeuterated (d62)-dipalmitoyl phosphatidylcholine/H2O. Analysis of the deuterium spectra in terms of the moments of the spectra allows one to make quantit...

journal_title：Biophysical journal

authors： Davis JH

更新日期：1979-09-01 00:00:00

abstract：:Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish...

journal_title：Biophysical journal

authors： Barnhart EL,Allen GM,Jülicher F,Theriot JA

更新日期：2010-03-17 00:00:00

abstract：:We have used identified neurons from the abdominal ganglion of the mollusc Aplysia to construct and analyze two circuits in vitro. Each of these circuits was capable of producing two patterns of persistent activity; that is, they had bistable output states. The output could be switched between the stable states by a b...

journal_title：Biophysical journal

authors： Kleinfeld D,Raccuia-Behling F,Chiel HJ

更新日期：1990-04-01 00:00:00

abstract：:We report the crystal structure of a bromide-bound form of the D85S mutant of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather than ATP to pump halide ions across the cell membrane. Comparison of the structure of the halide-bound and halide-free states reveals that both displacements of individual ...

journal_title：Biophysical journal

authors： Facciotti MT,Cheung VS,Nguyen D,Rouhani S,Glaeser RM

更新日期：2003-07-01 00:00:00

abstract：:Two-dimensional electronic chirality-induced signals of excitons in the photosynthetic Fenna-Matthews-Olson complex from two species of green sulfur bacteria (Chlorobium tepidum and Prosthecochloris aestuarii) are compared. The spectra are predicted to provide sensitive probes of local protein environment of the const...

journal_title：Biophysical journal

authors： Voronine DV,Abramavicius D,Mukamel S

更新日期：2008-11-15 00:00:00

abstract：:Cell adhesion often occurs under dynamic conditions, as in flowing blood. A quantitative understanding of this process requires accurate knowledge of the topographical relationships between the cell membrane and potentially adhesive surfaces. This report describes an experimental study made on both the translational a...

journal_title：Biophysical journal

authors： Tissot O,Pierres A,Foa C,Delaage M,Bongrand P

更新日期：1992-01-01 00:00:00

abstract：:Molecular dynamics (MD) simulations of 5 ns on protein-DNA complexes of catabolite-activator protein (CAP), lambda-repressor, and their corresponding uncomplexed protein and DNA, are reported. These cases represent two extremes of DNA bending, with CAP DNA bent severely and the lambda-operator nearly straight when com...

journal_title：Biophysical journal

authors： Dixit SB,Andrews DQ,Beveridge DL

更新日期：2005-05-01 00:00:00

abstract：:Gap junctions formed of connexin46 (Cx46) and connexin50 (Cx50) in lens fiber cells are crucial for maintaining lens transparency. We determined the functional properties of homotypic Cx46, heterotypic Cx46/Cx50, and heteromeric Cx46/Cx50 channels in a communication-deficient neuroblastoma (N2A) cell line, using dual ...

journal_title：Biophysical journal

authors： Hopperstad MG,Srinivas M,Spray DC

更新日期：2000-10-01 00:00:00

abstract：:We have worked out a procedure for covalent binding of native biomacromolecules on flat gold surfaces for scanning probe microscopy in aqueous buffer solutions and for other nanotechnological applications, such as the direct measurement of interaction forces between immobilized macromolecules, of their elastomechanica...

journal_title：Biophysical journal

authors： Wagner P,Hegner M,Kernen P,Zaugg F,Semenza G

更新日期：1996-05-01 00:00:00

abstract：:The polyunsaturated fatty acid docosahexaenoic acid (DHA) makes up approximately 50% of the lipid chains in the retinal rod outer segment disk membranes and a large fraction of the lipid chains in the membranes of neuronal tissues. There is an extensive literature concerned with the dietary requirements for essential ...

journal_title：Biophysical journal

authors： Everts S,Davis JH

更新日期：2000-08-01 00:00:00

abstract：:The glutamate-activated current in photoreceptors has been attributed both to a sodium/glutamate transporter and to a glutamate-activated chloride channel. We have further studied the glutamate-activated current in single, isolated photoreceptors from the tiger salamander using noise analysis on whole-cell patch-clamp...

journal_title：Biophysical journal

authors： Larsson HP,Picaud SA,Werblin FS,Lecar H

更新日期：1996-02-01 00:00:00

abstract：:Closing linear poly(ethylene glycol) (PEG) into a circular "crown" dramatically changes its dynamics in the alpha-hemolysin channel. In the electrically neutral crown ether (C2H4O)6, six ethylene oxide monomers are linked into a circle that gives the molecule ion-complexing capacity and increases its rigidity. As with...

journal_title：Biophysical journal

authors： Bezrukov SM,Krasilnikov OV,Yuldasheva LN,Berezhkovskii AM,Rodrigues CG

更新日期：2004-11-01 00:00:00

abstract：:The recently developed method of site-directed Fourier transform infrared dichroism for obtaining orientational constraints of oriented polymers is applied here to the transmembrane domain of the vpu protein from the human immunodeficiency virus type 1 (HIV-1). The infrared spectra of the 31-residue-long vpu peptide r...

journal_title：Biophysical journal

authors： Kukol A,Arkin IT

更新日期：1999-09-01 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:The cell membrane of Salinibacter ruber contains xanthorhodopsin, a light-driven transmembrane proton pump with two chromophores: a retinal and the carotenoid, salinixanthin. Action spectra for transport had indicated that light absorbed by either is utilized for function. If the carotenoid is an antenna in this prote...

journal_title：Biophysical journal

authors： Balashov SP,Imasheva ES,Wang JM,Lanyi JK

更新日期：2008-09-01 00:00:00

abstract：:We have developed a model for the analysis of the forward creatine kinase reaction in muscle as measured by the nuclear magnetic resonance (NMR) technique of magnetization transfer. The model, accounting for the double-exponential behavior observed in some NMR magnetization transfer data, allows for the existence of t...

journal_title：Biophysical journal

authors： Zahler R,Bittl JA,Ingwall JS

更新日期：1987-06-01 00:00:00

abstract：:The effect of salt and pH titration on the selectivity of spin-labeled analogues of phosphatidic acid, phosphatidylserine, phosphatidylcholine, and stearic acid for the nicotinic acetylcholine receptor (nAcChoR) reconstituted into dioleoylphosphatidylcholine was examined at 0 degrees C using electron spin resonance sp...

journal_title：Biophysical journal

authors： Raines DE,Miller KW

更新日期：1993-03-01 00:00:00

abstract：:We have established a quantum mechanics (QM)/molecular mechanics (MM) hybrid method for calculating the Raman spectra of protein-bound cofactors using the alpha-subunit of C-phycocyanin containing a phycocyanobilin (PCB) chromophore as a test case. The PCB cofactor was described with density functional theory, whereas...

journal_title：Biophysical journal

authors： Mroginski MA,Mark F,Thiel W,Hildebrandt P

更新日期：2007-09-15 00:00:00

abstract：:To explain the effect of profilin on actin critical concentration in a manner consistent with thermodynamic constraints and available experimental data, we built a thermodynamically rigorous model of actin steady-state dynamics in the presence of profilin. We analyzed previously published mechanisms theoretically and ...

journal_title：Biophysical journal

authors： Yarmola EG,Dranishnikov DA,Bubb MR

更新日期：2008-12-15 00:00:00

abstract：:The current rapid expansion of biological knowledge offers a great opportunity to rationally engineer biological systems that respond to signals such as light and chemical inducers by producing specific proteins. Turning on and off the production of proteins on demand holds great promise for creating significant biote...

journal_title：Biophysical journal

authors： Tuttle LM,Salis H,Tomshine J,Kaznessis YN

更新日期：2005-12-01 00:00:00

abstract：:Using a combination of microelectrode measurements and high-power microscopy we have demonstrated that different Donnan potentials can be recorded from the A- and I-bands of glycerinated and chemically skinned muscles in rigor, so that the A-band fixed charge concentration exceeds the I-band fixed charge concentration...

journal_title：Biophysical journal

authors： Bartels EM,Elliott GF

更新日期：1985-07-01 00:00:00