Abstract:
:The lipid matrix present in the uppermost layer of the skin, the stratum corneum, plays a crucial role in the skin barrier function. The lipids are organized into two lamellar phases. To gain more insight into the molecular organization of one of these lamellar phases, we performed neutron diffraction studies. In the diffraction pattern, five diffraction orders were observed attributed to a lamellar phase with a repeat distance of 5.4 nm. Using contrast variation, the scattering length density profile could be calculated showing a typical bilayer arrangement. To obtain information on the arrangement of ceramides in the unit cell, a mixture that included a partly deuterated ceramide was also examined. The scattering length density profile of the 5.4-nm phase containing this deuterated ceramide demonstrated a symmetric arrangement of the ceramides with interdigitating acyl chains in the center of the unit cell.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Groen D,Gooris GS,Barlow DJ,Lawrence MJ,van Mechelen JB,Demé B,Bouwstra JAdoi
10.1016/j.bpj.2011.02.001subject
Has Abstractpub_date
2011-03-16 00:00:00pages
1481-9issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(11)00187-1journal_volume
100pub_type
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