Abstract:
:Slow fluorescence transients in Chlamydomonas reinhardi arise after transitions from high light intensities to low light or dark conditions. Characteristics of the newly described transient phenomena include: (a) A slow biphasic decrease in fluorescence yield occurs in the dark, followed by an even slower, hour long, increase in fluorescence. (b) A similar, but faster, fluorescence yield decrease and subsequent increase also occurs during low intensity illumination periods separating high light intervals, or after transitions from high intensity to low intensity light. (c) Short (several seconds) flashes of light given during a dark period have no effect on the dark fluorescence decay, regardless of the flash frequency. Such flash regimes accurately monitor the dark decline of the M2 level by tracing the parallel decay of flash-generated P2 (Kautsky) peaks. However, flashes during a low light illumination period do influence the decay kinetics. Frequent flashes allow decay similar to that occurring in dark, but less frequent flashes inhibit the decrease in fluorescence yield.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Rice GE,Bannister TTdoi
10.1016/S0006-3495(80)85015-6subject
Has Abstractpub_date
1980-11-01 00:00:00pages
767-77issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(80)85015-6journal_volume
32pub_type
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