Abstract:
:We apply topography and recognition (TREC) imaging to the analysis of whole, untreated human tissue for what we believe to be the first time. Pseudoexfoliation syndrome (PEX), a well-known cause of irreversible blindness worldwide, is characterized by abnormal protein aggregation on the anterior lens capsule of the eye. However, the development of effective therapies has been hampered by a lack of detailed knowledge of the protein constituents in these pathological deposits and their distribution. Using both TREC and immunofluorescence, one of the proteins implicated in the PEX pathology--the apolipoprotein clusterin--was detected, and differences in its distribution pattern on the surface of untreated human lens capsule tissue in both PEX and normal control samples were investigated. Our study shows the potential of TREC imaging for the analysis of whole, untreated human tissue samples.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Creasey R,Sharma S,Craig JE,Gibson CT,Ebner A,Hinterdorfer P,Voelcker NHdoi
10.1016/j.bpj.2010.06.044subject
Has Abstractpub_date
2010-09-08 00:00:00pages
1660-7issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(10)00787-3journal_volume
99pub_type
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