Detecting protein aggregates on untreated human tissue samples by atomic force microscopy recognition imaging.

Abstract:

:We apply topography and recognition (TREC) imaging to the analysis of whole, untreated human tissue for what we believe to be the first time. Pseudoexfoliation syndrome (PEX), a well-known cause of irreversible blindness worldwide, is characterized by abnormal protein aggregation on the anterior lens capsule of the eye. However, the development of effective therapies has been hampered by a lack of detailed knowledge of the protein constituents in these pathological deposits and their distribution. Using both TREC and immunofluorescence, one of the proteins implicated in the PEX pathology--the apolipoprotein clusterin--was detected, and differences in its distribution pattern on the surface of untreated human lens capsule tissue in both PEX and normal control samples were investigated. Our study shows the potential of TREC imaging for the analysis of whole, untreated human tissue samples.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Creasey R,Sharma S,Craig JE,Gibson CT,Ebner A,Hinterdorfer P,Voelcker NH

doi

10.1016/j.bpj.2010.06.044

subject

Has Abstract

pub_date

2010-09-08 00:00:00

pages

1660-7

issue

5

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(10)00787-3

journal_volume

99

pub_type

杂志文章
  • Lipid-Composition-Mediated Forces Can Stabilize Tubular Assemblies of I-BAR Proteins.

    abstract::Collective action by inverse-Bin/Amphiphysin/Rvs (I-BAR) domains drive micron-scale membrane remodeling. The macroscopic curvature sensing and generation behavior of I-BAR domains is well characterized, and computational models have suggested various mechanisms on simplified membrane systems, but there remain missing ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2020.11.019

    authors: Jarin Z,Pak AJ,Bassereau P,Voth GA

    更新日期:2021-01-05 00:00:00

  • Theoretical basis for an anomalous temperature coefficient in swelling pressure of rabbit corneal stroma.

    abstract::In the rabbit corneal stroma, the swelling pressure, P, has been reported to have an anomalous (negative) temperature coefficient, alpha P, contradicting traditional Donnan swelling theory. A parallel-plate, diffuse double layer Gouy-Chapman model was used to resolve this discrepancy. The present model incorporates th...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(90)82584-4

    authors: Kwok LS,Klyce SD

    更新日期:1990-03-01 00:00:00

  • Viscoelastic dynamics of actin filaments coupled to rotary F-ATPase: curvature as an indicator of the torque.

    abstract::ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(01)75781-5

    authors: Cherepanov DA,Junge W

    更新日期:2001-09-01 00:00:00

  • Fluorescence study of the divalent cation-transport mechanism of ionophore A23187 in phospholipid membranes.

    abstract::The mechanism for transport of divalent cations across phospholipid bilayers by the ionophore A23187 was investigated. The intrinsic fluorescence of the ionophore was used in equilibrium and rapid-mixing experiments as an indicator of ionophore environment and complexation with divalent cations. The neutral (protonate...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(81)84738-8

    authors: Kolber MA,Haynes DH

    更新日期:1981-11-01 00:00:00

  • Free energy determinants of binding the rRNA substrate and small ligands to ricin A-chain.

    abstract::A continuum model is provided of the free energy terms that contribute to the molecular association of ricin A-chain (RTA) with the rRNA substrate and several small ligands. The model for RTA interactions with the RNA was taken from a previously proposed complex containing a 29-mer oligonucleotide hairpin (. Proteins ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(99)77175-4

    authors: Olson MA,Cuff L

    更新日期:1999-01-01 00:00:00

  • Membrane on a chip: a functional tethered lipid bilayer membrane on silicon oxide surfaces.

    abstract::Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.105.061374

    authors: Atanasov V,Knorr N,Duran RS,Ingebrandt S,Offenhäusser A,Knoll W,Köper I

    更新日期:2005-09-01 00:00:00

  • Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction.

    abstract::Many aspects of chromatin biology are influenced by the nuclear compartment in which a locus resides, from transcriptional regulation to DNA repair. Further, the dynamic and variable localization of a particular locus across cell populations and over time makes analysis of a large number of cells critical. As a conseq...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2016.05.036

    authors: Zhao Y,Schreiner SM,Koo PK,Colombi P,King MC,Mochrie SG

    更新日期:2016-07-12 00:00:00

  • P-chloromercuribenzene sulfonate blocks and reverses the effect of amiloride on sodium transport across rabbit colon in vitro.

    abstract::The addition of 10(-3) M p-chloromercuribenzene sulfonate (PCMBS) to the solution bathing the mucosal surface of rabbit colon has no effect on the rate of active Na transport but blocks or reverses the inhibitory action of amiloride. The tissue must be exposed to PCMBS for 20-30 min for a complete blocking effect, and...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(78)85477-0

    authors: Gottlieb GP,Turnheim K,Frizzell RA,Schultz SG

    更新日期:1978-04-01 00:00:00

  • Patch-clamp recording of charge movement, Ca2+ current, and Ca2+ transients in adult skeletal muscle fibers.

    abstract::Intramembrane charge movement (Q), Ca(2+) conductance (G(m)) through the dihydropyridine-sensitive L-type Ca(2+) channel (DHPR) and intracellular Ca(2+) fluorescence (F) have been recorded simultaneously in flexor digitorum brevis muscle fibers of adult mice, using the whole-cell configuration of the patch-clamp techn...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/s0006-3495(99)77104-3

    authors: Wang ZM,Messi ML,Delbono O

    更新日期:1999-11-01 00:00:00

  • Membrane fusion and the lamellar-to-inverted-hexagonal phase transition in cardiolipin vesicle systems induced by divalent cations.

    abstract::The polymorphic phase behavior of bovine heart cardiolipin (CL) in the presence of different divalent cations and the kinetics of CL vesicle fusion induced by these cations have been investigated. (31)P-NMR measurements of equilibrium cation-CL complexes showed the lamellar-to-hexagonal (L(alpha)-H(II)) transition tem...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(99)77041-4

    authors: Ortiz A,Killian JA,Verkleij AJ,Wilschut J

    更新日期:1999-10-01 00:00:00

  • Temperature-dependent electrical and ultrastructural characterizations of porcine skin upon electroporation.

    abstract::The mechanism of high-voltage pulse-induced permeabilization of the stratum corneum, the outer layer of the skin, is still not completely understood. It has been suggested that joule heating resulting from the applied pulse may play a major role in disrupting the stratum corneum. In this study, electrical and ultrastr...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(02)75378-2

    authors: Gallo SA,Sen A,Hensen ML,Hui SW

    更新日期:2002-01-01 00:00:00

  • Resolution of multiphasic reactions by the combination of fluorescence total-intensity and anisotropy stopped-flow kinetic experiments.

    abstract::Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interr...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(94)80741-6

    authors: Otto MR,Lillo MP,Beechem JM

    更新日期:1994-12-01 00:00:00

  • Dynamic imaging of lateral diffusion by electron spin resonance and study of rotational dynamics in model membranes. Effect of cholesterol.

    abstract::The effects of cholesterol on the dynamics and the structural properties of two different spin probes, the sterol type CSL and the phospholipid type 16-PC, in POPC/cholesterol oriented multilayer model membranes were examined. Our results are consistent with a nonideal solution containing cholesterol-rich clusters cre...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(89)82847-4

    authors: Shin YK,Freed JH

    更新日期:1989-03-01 00:00:00

  • A model of phosphofructokinase and glycolytic oscillations in the pancreatic beta-cell.

    abstract::We have constructed a model of the upper part of the glycolysis in the pancreatic beta-cell. The model comprises the enzymatic reactions from glucokinase to glyceraldehyde-3-phosphate dehydrogenase (GAPD). Our results show, for a substantial part of the parameter space, an oscillatory behavior of the glycolysis for a ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(03)74460-9

    authors: Westermark PO,Lansner A

    更新日期:2003-07-01 00:00:00

  • F-actin, a model polymer for semiflexible chains in dilute, semidilute, and liquid crystalline solutions.

    abstract::Single actin filaments were analyzed in solutions ranging from dilute (0.2 microgram/ml), where filaments interact only with solvent, to concentrations (4.0 mg/ml) at which F-actin forms a nematic phase. A persistence length of approximately 1.8 microns and an average length of approximately 22 microns (Kaufmann et al...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(96)79630-3

    authors: Käs J,Strey H,Tang JX,Finger D,Ezzell R,Sackmann E,Janmey PA

    更新日期:1996-02-01 00:00:00

  • On the minimization of fluctuations in the response times of autoregulatory gene networks.

    abstract::The temporal dynamics of the concentrations of several proteins are tightly regulated, particularly for critical nodes in biological networks such as transcription factors. An important mechanism to control transcription factor levels is through autoregulatory feedback loops where the protein can bind its own promoter...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2011.08.005

    authors: Murugan R,Kreiman G

    更新日期:2011-09-21 00:00:00

  • Analysis of a Functional Dimer Model of Ubiquinol Cytochrome c Oxidoreductase.

    abstract::Ubiquinol cytochrome c oxidoreductase (bc1 complex) serves as an important electron junction in many respiratory systems. It funnels electrons coming from NADH and ubiquinol to cytochrome c, but it is also capable of producing significant amounts of the free radical superoxide. In situ and in other experimental system...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2017.08.018

    authors: Bazil JN

    更新日期:2017-10-03 00:00:00

  • Carboxyl tail prevents yeast K(+) channel closure: proposal of an integrated model of TOK1 gating.

    abstract::TOK1 encodes the channel responsible for the prominent outward K(+) current of the yeast plasma membrane. It can dwell in several impermeable states, including a rapidly transiting, K(+)-electromotive-force-dependent "R" (rectifying) state, a voltage-independent "IB" (interburst) state, and a set of [K(+)](ext) and vo...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(02)75440-4

    authors: Loukin SH,Saimi Y

    更新日期:2002-02-01 00:00:00

  • Dipole-Potential-Mediated Effects on Ion Pump Kinetics.

    abstract::The kinetics of conformational changes of P-type ATPases necessary for the occlusion or deocclusion of transported ions are known to be sensitive to the composition of the surrounding membrane, e.g., phospholipid content, mole percentage of cholesterol, and the presence of lipid-bound anions. Research has shown that m...

    journal_title:Biophysical journal

    pub_type: 杂志文章,评审

    doi:10.1016/j.bpj.2015.08.022

    authors: Clarke RJ

    更新日期:2015-10-20 00:00:00

  • Comparative Fourier transform infrared spectroscopy study of cold-, pressure-, and heat-induced unfolding and aggregation of myoglobin.

    abstract::We studied the cold unfolding of myoglobin with Fourier transform infrared spectroscopy and compared it with pressure and heat unfolding. Because protein aggregation is a phenomenon with medical as well as biotechnological implications, we were interested in both the structural changes as well as the aggregation behav...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(02)75605-1

    authors: Meersman F,Smeller L,Heremans K

    更新日期:2002-05-01 00:00:00

  • Effect of anisotropic reactivity on the rate of diffusion-controlled reactions: comparative analysis of the models of patches and hemispheres.

    abstract::A comparative analysis of two models of anisotropic reactivity in bimolecular diffusion-controlled reaction kinetics is presented. One is the conventional model of reactive patches (MRP), where the surface of a molecule is assumed to be reactive over a certain region (circular patch) with the rest of the surface being...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(01)76180-2

    authors: Barzykin AV,Shushin AI

    更新日期:2001-05-01 00:00:00

  • Quantum yield and image contrast of bacteriochlorophyll monolayers in photoelectron microscopy.

    abstract::The photoelectron quantum yield spectrum of bacteriochlorophyll aGg (Bchl a ) from Rhodospirillum rubrum was determined in order to evaluate the possibility of mapping photoreceptor distribution and organization in bacterial chromatophores. The quantum yield is on the order of 1 X 10(-3) electrons/incident photon at 1...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(78)85519-2

    authors: Barnes RB,Amend J,Sistrom WR,Griffith OH

    更新日期:1978-03-01 00:00:00

  • Stability constant of the 1:1 complex of sodium with guanosine 5'-monophosphate.

    abstract::The stability of the 1:1 complex of sodium ion with the dianion of guanosine 5'-monophosphate has been determined by means of a potentiometric titration employing a specific ion electrode. The stability constant for the reaction Na(+) + 5'-GMP(2-) Na(5'-GMP)(-) was found to be 2.85 +/- 0.36 M(-1) at 5 degrees C and an...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/s0006-3495(82)84564-5

    authors: Walmsley JA,Pinnavaia TJ

    更新日期:1982-06-01 00:00:00

  • Three-dimensional modeling of the brain's ECS by minimum configurational energy packing of fluid vesicles.

    abstract::The extracellular space of the brain is the heterogeneous porous medium formed by the spaces between the brain cells. Diffusion in this interstitial space is the mechanism by which glucose and oxygen are delivered to the brain cells from the vascular system. It is also a medium for the transport of certain information...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.106.095547

    authors: Nandigam RK,Kroll DM

    更新日期:2007-05-15 00:00:00

  • The EF loop in green proteorhodopsin affects conformation and photocycle dynamics.

    abstract::The proteorhodopsin family consists of retinal proteins of marine bacterial origin with optical properties adjusted to their local environments. For green proteorhodopsin, a highly specific mutation in the EF loop, A178R, has been found to cause a surprisingly large redshift of 20 nm despite its distance from the chro...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2013.06.014

    authors: Mehler M,Scholz F,Ullrich SJ,Mao J,Braun M,Brown LJ,Brown RC,Fiedler SA,Becker-Baldus J,Wachtveitl J,Glaubitz C

    更新日期:2013-07-16 00:00:00

  • Continuous allosteric regulation of a viral packaging motor by a sensor that detects the density and conformation of packaged DNA.

    abstract::We report evidence for an unconventional type of allosteric regulation of a biomotor. We show that the genome-packaging motor of phage ϕ29 is regulated by a sensor that detects the density and conformation of the DNA packaged inside the viral capsid, and slows the motor by a mechanism distinct from the effect of a dir...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2014.11.3469

    authors: Berndsen ZT,Keller N,Smith DE

    更新日期:2015-01-20 00:00:00

  • An atomistic view on human hemoglobin carbon monoxide migration processes.

    abstract::A significant amount of work has been devoted to obtaining a detailed atomistic knowledge of the human hemoglobin mechanism. Despite this impressive research, to date, the ligand diffusion processes remain unclear and controversial. Using recently developed computational techniques, PELE, we are capable of addressing ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2012.01.011

    authors: Lucas MF,Guallar V

    更新日期:2012-02-22 00:00:00

  • The Contribution of the Ankyrin Repeat Domain of TRPV1 as a Thermal Module.

    abstract::The TRPV1 cation nonselective ion channel plays an essential role in thermosensation and perception of other noxious stimuli. TRPV1 can be activated by low extracellular pH, high temperature, or naturally occurring pungent molecules such as allicin, capsaicin, or resiniferatoxin. Its noxious thermal sensitivity makes ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2019.10.041

    authors: Ladrón-de-Guevara E,Dominguez L,Rangel-Yescas GE,Fernández-Velasco DA,Torres-Larios A,Rosenbaum T,Islas LD

    更新日期:2020-02-25 00:00:00

  • Resonant and localized breathing modes in terminal regions of the DNA double helix.

    abstract::A Green's function approach is used in constructing a dynamic model of a semi-infinite length of the DNA homopolymer B poly(d) . poly(d). Considerable attention is focused on the hydrogen bond stretching close to the terminus. A melting (or breathing) coordinate (M) is defined as an average over the three linking hydr...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(81)84789-3

    authors: Putnam BF,Van Zandt LL,Prohofsky EW,Mei WN

    更新日期:1981-08-01 00:00:00

  • Torque generation by the flagellar rotary motor.

    abstract::A review is given of the structure and dynamics of the flagellar rotary motor. Force-generating elements in a motor driving a tethered bacterium (a cell fixed to the substratum by a single flagellum) exert forces of order 20 pN while moving at speeds of order 1 micron/s. Force-generating elements in a motor driving a ...

    journal_title:Biophysical journal

    pub_type: 杂志文章,评审

    doi:

    authors: Berg HC

    更新日期:1995-04-01 00:00:00