Statistical thermodynamics and kinetics of DNA multiplex hybridization reactions.

abstract：:Biofilm infections can consist of bacterial aggregates that are an order of magnitude larger than neutrophils, phagocytic immune cells that densely surround aggregates but do not enter them. Because a neutrophil is too small to engulf the entire aggregate, it must be able to detach and engulf a few bacteria at a time ...

journal_title：Biophysical journal

authors： Davis-Fields M,Bakhtiari LA,Lan Z,Kovach KN,Wang L,Cosgriff-Hernandez EM,Gordon VD

更新日期：2019-10-15 00:00:00

abstract：:Mössbauer spectra of anhydrohemoglobin establish the existence of two ferrous iron spin states in anhydrohemoglobin. Magnetic susceptibility measurements show that anhydrohemoglobin is paramagnetic and has an effective magnetic moment per iron atom of 3.5+/-0.1mu(B) at low temperatures. In conjunction with the suscept...

journal_title：Biophysical journal

authors： Grant RW,Cape JA,Gonser U,Topol LE,Saltman P

更新日期：1967-11-01 00:00:00

abstract：:Cytotoxic lymphocytes are key elements of the immune system that are primarily responsible for targeting cells infected with intracellular pathogens, or cells that have become malignantly transformed. Target cells are killed mainly via lymphocyte exocytosis of specialized lysosomes containing perforin, a pore-forming ...

journal_title：Biophysical journal

authors： Woodsworth DJ,Dunsing V,Coombs D

更新日期：2015-08-04 00:00:00

abstract：:Molecular dynamics simulations have been applied to the DNA octamer d(GCGCA-GAAC). d(GTTCGCGC), which has an adenine bulge at the center to determine the pathway for interconversion between the stacked and extended forms. These forms are known to be important in the molecular recognition of bulges. From a total of ~35...

journal_title：Biophysical journal

authors： Feig M,Zacharias M,Pettitt BM

更新日期：2001-07-01 00:00:00

abstract：:A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found for the various parts of the life cycle: S, 4.13 hours; G1, 4.71 hours; G2, 2.81 hours; mitosis, 0.81 hours. The cell has a to...

journal_title：Biophysical journal

authors： PUCK TT,SANDERS P,PETERSEN D

更新日期：1964-11-01 00:00:00

abstract：:The cytoplasm of a living cell is a dynamic environment through which intracellular components must move and mix. In motile, rapidly deforming cells such as human neutrophils, bulk cytoplasmic flow couples cell deformation to the transport and dispersion of cytoplasmic particles. Using particle-tracking measurements i...

journal_title：Biophysical journal

authors： Koslover EF,Chan CK,Theriot JA

更新日期：2017-11-07 00:00:00

abstract：:Investigations into the spatiotemporal dynamics of DNA repair using live-cell imaging are aided by the ability to generate well defined regions of ultravioletlike photolesions in an optical microscope. We demonstrate that multiphoton excitation of DNA in live cells with visible femtosecond pulses produces thymine cycl...

journal_title：Biophysical journal

authors： Daddysman MK,Fecko CJ

更新日期：2011-11-02 00:00:00

abstract：:We demonstrate the ability of pulsed dipolar electron spin resonance (ESR) spectroscopy (PDS) to report on the conformation of Cu-Zn superoxide dismutase (SOD1) through the sensitive measurement of dipolar interactions between inherent Cu(2+) ions. Although the extent and the anisotropy of the Cu ESR spectrum provides...

journal_title：Biophysical journal

authors： Merz GE,Borbat PP,Pratt AJ,Getzoff ED,Freed JH,Crane BR

更新日期：2014-10-07 00:00:00

abstract：:Recent studies have demonstrated that caffeine can act as an antimutagen and inhibit the cytoxic and/or cytostatic effects of some DNA intercalating agents. It has been suggested that this inhibitory effect may be due to complexation of the DNA intercalator with caffeine. In this study we employ optical absorption, fl...

journal_title：Biophysical journal

authors： Larsen RW,Jasuja R,Hetzler RK,Muraoka PT,Andrada VG,Jameson DM

更新日期：1996-01-01 00:00:00

abstract：:Observation of cell membrane buckling and cell folding in micropipette aspiration experiments was used to evaluate the bending rigidity of the red blood cell membrane. The suction pressure required to buckle the membrane surface initially was found to be about one-half to two-thirds of the pressure that caused the cel...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1983-07-01 00:00:00

abstract：:We use optical tweezers microrheology and fluorescence microscopy to apply nonlinear microscale strains to entangled and cross-linked actin networks, and measure the resulting stress and actin filament deformations. We couple nonlinear stress response and relaxation to the velocities and displacements of individual fl...

journal_title：Biophysical journal

authors： Gurmessa B,Ricketts S,Robertson-Anderson RM

更新日期：2017-10-03 00:00:00

abstract：:Ionic channels bathed in mixed solutions of two permeant electrolytes often conduct less current than channels bathed in pure solutions of either. For many years, this anomalous mole fraction effect (AMFE) has been thought to occur only in single-file pores containing two or more ions at a time. Most thinking about ch...

journal_title：Biophysical journal

authors： Nonner W,Chen DP,Eisenberg B

更新日期：1998-05-01 00:00:00

abstract：:The effect of the presence of a minor antenna component in light-harvesting complexes of photosynthetic bacteria is investigated with numerical simulation employing the transition probability matrix method. A model antenna system of hexagonal symmetry is adopted, using as a working hypothesis that the minor component ...

journal_title：Biophysical journal

authors： Fischer MR,Hoff AJ

更新日期：1992-10-01 00:00:00

abstract：:A new stable substrate applicable to the observation of DNA molecules by atomic force microscopy (AFM) was fabricated from a ultrasmooth sapphire (alpha-Al2O3 single crystal) plate. The atomically ultrasmooth sapphire as obtained by high-temperature annealing has hydrophobic surfaces and could not be used for the AFM ...

journal_title：Biophysical journal

authors： Yoshida K,Yoshimoto M,Sasaki K,Ohnishi T,Ushiki T,Hitomi J,Yamamoto S,Sigeno M

更新日期：1998-04-01 00:00:00

abstract：:Long time series of monosynaptic Ia-afferent to alpha-motoneuron reflexes were recorded in the L7 or S1 ventral roots in the cat. Time series were collected before and after spinalization at T13 during constant amplitude stimulations of group Ia muscle afferents in the triceps surae muscle nerves. Using autocorrelatio...

journal_title：Biophysical journal

authors： Chang T,Schiff SJ,Sauer T,Gossard JP,Burke RE

更新日期：1994-08-01 00:00:00

abstract：:Collagen fibrils form extracellular networks that regulate cell functions and provide mechanical strength to tissues. Collagen fibrillogenesis is an entropy-driven process promoted by warming and reversed by cooling. Here, we investigate the influence of noncovalent interactions mediated by the collagen triple helix o...

journal_title：Biophysical journal

authors： de Wild M,Pomp W,Koenderink GH

更新日期：2013-07-02 00:00:00

abstract：:Lipid mixing between vesicles functionalized with SNAREs and the cytosolic C2AB domain of synaptotagmin-1 recapitulates the basic Ca(2+) dependence of neuronal exocytosis. However, in the conventional ensemble lipid mixing assays it is not possible to discriminate whether Ca(2+) accelerates the docking or the fusion o...

journal_title：Biophysical journal

authors： Christensen SM,Mortensen MW,Stamou DG

更新日期：2011-02-16 00:00:00

abstract：:Here we present a model for maltodextrin translocation through maltoporin channels. In a first step, our theoretical analysis does consider the case of a single binding site for a given substrate in a structurally unaffected channel with a possibly different entrance barrier on either side. It is shown how by means of...

journal_title：Biophysical journal

authors： Schwarz G,Danelon C,Winterhalter M

更新日期：2003-05-01 00:00:00

abstract：:Helicobacter pylori arginase, a bimetallic enzyme, is crucial for pathogenesis of the bacterium in human stomach. Despite conservation of the signature motifs in all arginases, the H. pylori homolog has a non-conserved motif (153ESEEKAWQKLCSL165), whose role was recently shown to be critical for its stability and func...

journal_title：Biophysical journal

authors： George G,Kombrabail M,Raninga N,Sau AK

更新日期：2017-03-28 00:00:00

abstract：:We present a detailed motion analysis of retrograde nerve growth factor (NGF) endosomes in axons to show that mechanical tugs-of-war and intracellular motor regulation are complimentary features of the near-unidirectional endosome directionality. We used quantum dots to fluorescently label NGF and acquired trajectorie...

journal_title：Biophysical journal

authors： Chowdary PD,Che DL,Zhang K,Cui B

更新日期：2015-06-02 00:00:00

abstract：:The process by which an organism changes the composition of its membranal fatty acids in response to growth temperature, so as to maintain optimal membrane functioning, is known as homeoviscous adaptation (HA). One expression of HA is the constancy of the fluorescence polarization (P) of the lipophilic probe 1,6-diphe...

journal_title：Biophysical journal

authors： Parola AH,Ibdah M,Gill D,Zaritsky A

更新日期：1990-03-01 00:00:00

abstract：:We present a simple two-parameter model for surface charge directly associated with ion channels. A spherically symmetric "charged shell" models a distribution of surface charge arrayed about the channel entrance, with a corresponding set of image charges behind the plane of the membrane. The transition between a regi...

journal_title：Biophysical journal

authors： Naranjo D,Latorre R,Cherbavaz D,McGill P,Schumaker MF

更新日期：1994-01-01 00:00:00

abstract：:The contribution of a specific hydrogen bond in apoflavodoxin to protein stability is investigated by combining theory, experiment and simulation. Although hydrogen bonds are major determinants of protein structure and function, their contribution to protein stability is still unclear and widely debated. The best meth...

journal_title：Biophysical journal

authors： Campos LA,Cuesta-López S,López-Llano J,Falo F,Sancho J

更新日期：2005-02-01 00:00:00

abstract：:Xenopus oocytes are widely employed for heterologous expression of cloned proteins, particularly electrogenic molecules such as ion channels and transporters. The high levels of expression readily obtained permit detailed investigations without interference from endogenous conductances. Injection of min K mRNA into Xe...

journal_title：Biophysical journal

authors： Tzounopoulos T,Maylie J,Adelman JP

更新日期：1995-09-01 00:00:00

abstract：:Recent studies have indicated that the tip links and kinocilial links of sensory hair bundles in the inner ear have similar properties and share a common epitope, and that cadherin 23 may also be a component of each link type. Transmission electron microscopy was therefore used to study and compare the fine structure ...

journal_title：Biophysical journal

authors： Tsuprun V,Goodyear RJ,Richardson GP

更新日期：2004-12-01 00:00:00

abstract：:Mechanical and two-dimensional (2D) x-ray diffraction studies suggest that during isometric steady-state contraction, strongly bound cross-bridges mostly occupy early states in the power stroke, whereas rigor or rigor-like cross-bridges could not be detected. However, it remained unclear whether cross-bridges accumula...

journal_title：Biophysical journal

authors： Radocaj A,Weiss T,Helsby WI,Brenner B,Kraft T

更新日期：2009-02-18 00:00:00

abstract：:Catch force in molluscan smooth muscle requires little, if any, energy input and is controlled by the phosphorylation state of the thick filament-associated mini-titin, twitchin. The kinetic parameters of myosin cross-bridge turnover in permeabilized catch muscle, and how they are potentially modified by the catch mec...

journal_title：Biophysical journal

authors： Franke AS,Mooers SU,Narayan SR,Siegman MJ,Butler TM

更新日期：2007-07-15 00:00:00

abstract：:The structure of a membrane-embedded alpha-helical reference protein, the M13 major coat protein, is characterized under different conditions of hydrophobic mismatch using fluorescence resonance energy transfer in combination with high-throughput mutagenesis. We show that the structure is similar in both thin (14:1) a...

journal_title：Biophysical journal

authors： Vos WL,Schor M,Nazarov PV,Koehorst RB,Spruijt RB,Hemminga MA

更新日期：2007-11-15 00:00:00

abstract：:Computational methods that predict three-dimensional structures from amino acid sequences have become increasingly accurate and have provided insights into structure-function relationships for proteins in the absence of structural data. However, the accuracy of computational structural models requires experimental app...

journal_title：Biophysical journal

authors： Tateyama M,Liu H,Yang AS,Cormier JW,Kass RS

更新日期：2004-03-01 00:00:00

abstract：:The canonical mechanism of gramicidin (gA) channel formation is transmembrane dimerization of nonconducting subunits that reside in opposite bilayer leaflets. The channels do not open and close; they appear and disappear due to subunit association and dissociation. Many different types of experiments support this mono...

journal_title：Biophysical journal

authors： Lum K,Ingólfsson HI,Koeppe RE 2nd,Andersen OS

更新日期：2017-10-17 00:00:00