Myosin cross-bridge kinetics and the mechanism of catch.

abstract：:Phospholipids have been treated as dimers on a hexagonal lattice, and a move has been introduced that allows the dimers to move and change their orientation on the lattice. Simulations have been performed in which phospholipid chains have been treated as being either independent or infinitely coupled thermodynamically...

journal_title：Biophysical journal

authors： Jerala R,Almeida PF,Biltonen RL

更新日期：1996-08-01 00:00:00

abstract：:To understand the thermodynamic exclusion of Na(+) relative to K(+) from the S(2) site of the selectivity filter, the distribution P(X)(epsilon) (X = K(+) or Na(+)) of the binding energy (epsilon) of the ion with the channel is analyzed using the potential distribution theorem. By expressing the excess chemical potent...

journal_title：Biophysical journal

authors： Dixit PD,Merchant S,Asthagiri D

更新日期：2009-03-18 00:00:00

abstract：:The diffusion of Ca as it converges to the external mouth of a Ca channel is examined. Diffusional limitation on Ca ions entering Ca channels during current flow, cause local extracellular Ca depletions. Such extracellular Ca depletions have been reported in cardiac muscle. The cardiac sarcolemma has a large number of...

journal_title：Biophysical journal

authors： Bers DM,Peskoff A

更新日期：1991-03-01 00:00:00

abstract：:We have measured the static magnetization of unreduced and reduced reaction centers that vary in their quinone content. Measurements were performed in the temperature range 0.7 degrees K less than T less than 200 degrees K and magnetic fields of up to 10 kG. The electronic g-value, crystal field parameters D, E, and t...

journal_title：Biophysical journal

authors： Butler WF,Johnston DC,Shore HB,Fredkin DR,Okamura MY,Feher G

更新日期：1980-12-01 00:00:00

abstract：:The p7 protein from hepatitis C virus is critical for the assembly and secretion of infectious virus, making it an attractive drug target. It is thought to be a viroporin with a demonstrated ion channel activity when reconstituted into planar lipid bilayers. Electron microscopy experiments suggest that p7 oligomers co...

journal_title：Biophysical journal

authors： Chew CF,Vijayan R,Chang J,Zitzmann N,Biggin PC

更新日期：2009-01-01 00:00:00

abstract：:Atherosclerosis is a disease initiated by lipoprotein aggregation and deposition in artery walls. In this study, the de novo low-density lipoprotein aggregation process was examined. Nine major intermediates were identified in two stages of the aggregation process. In the aggregation stage, low-density lipoprotein mol...

journal_title：Biophysical journal

authors： Xu S,Lin B

更新日期：2001-10-01 00:00:00

abstract：:Alamethicin and its analogs from cation selective, multi-conductance channels in lipid bilayers. The conductance levels have been thought to be due to a barrel-stave structure where conducting pores (barrels) are formed by the self-assembly of a variable number of alpha-helical rods (staves). The conductance transitio...

journal_title：Biophysical journal

authors： Laver DR

更新日期：1994-02-01 00:00:00

abstract：:Stationary and kinetic experiments were performed on lipid bilayer membranes to study the mechanism of iodine- and bromine-mediated halide transport in detail. The stationary conductance data suggested that four different 1:1 complexes between I2 and Br2 and the halides I- and Br- were responsible for the observed con...

journal_title：Biophysical journal

authors： Klotz KH,Benz R

更新日期：1993-12-01 00:00:00

abstract：:The effect of transepithelial potential difference (psi) on Na and Cl flux across toad bladder was assessed by measuring isotopic flux between identical media at various values of psi. The contribution of edge damage to ionic permeability was eliminated, resulting in relatively high spontaneous psi (-97 +/-4 mv) and l...

journal_title：Biophysical journal

authors： Walser M

更新日期：1972-04-01 00:00:00

abstract：:A recipe is given for designing theoretical models for donor-acceptor systems in which fluorescence energy transfer and motion takes place simultaneously. This recipe is based on the idea that a system exhibiting both motion and fluorescence energy transfer can be modeled by specifying a number of "states" and the rat...

journal_title：Biophysical journal

authors： van der Meer BW,Raymer MA,Wagoner SL,Hackney RL,Beechem JM,Gratton E

更新日期：1993-04-01 00:00:00

abstract：:We present a detailed motion analysis of retrograde nerve growth factor (NGF) endosomes in axons to show that mechanical tugs-of-war and intracellular motor regulation are complimentary features of the near-unidirectional endosome directionality. We used quantum dots to fluorescently label NGF and acquired trajectorie...

journal_title：Biophysical journal

authors： Chowdary PD,Che DL,Zhang K,Cui B

更新日期：2015-06-02 00:00:00

abstract：:Effects of intracellular ionic strength on the isotonic contraction properties of both intact fibers and skinned fibers give insights into the cross-bridge mechanism, but presently there is fundamental disagreement in the results on the two fiber preparations. This paper, which studies the effects on contraction of va...

journal_title：Biophysical journal

authors： Gulati J,Babu A

更新日期：1984-02-01 00:00:00

abstract：:Starting from the Monod-Wyman-Changeux (MWC) model (Monod, J., J. Wyman, and J. P. Changeux. 1965. J. Mol. Biol. 12:88-118), we obtain an analytical expression for the slope of the Hill plot at any ligand concentration. Furthermore, we derive an equation satisfied by the ligand concentration at the position of maximum...

journal_title：Biophysical journal

authors： Zhou G,Ho PS,van Holde KE

更新日期：1989-02-01 00:00:00

abstract：:A kinetic model is proposed to reproduce the time courses of the concentration change in paused leader RNA, terminated leader RNA, and readthrough RNA in the single-round transcription experiments on trp leader region of Escherichia coli and its mutants, L132, L75, and L75L135 (Winkler, M. E., and C. Yanofsky, 1981, B...

journal_title：Biophysical journal

authors： Suzuki H,Kunisawa T,Otsuka J

更新日期：1987-03-01 00:00:00

abstract：:We measured isotonic sliding distance of single skinned fibers from rabbit psoas muscle when known and limited amounts of ATP were made available to the contractile apparatus. The fibers were immersed in paraffin oil at 20 degrees C, and laser pulse photolysis of caged ATP within the fiber initiated the contraction. T...

journal_title：Biophysical journal

authors： Higuchi H,Goldman YE

更新日期：1995-10-01 00:00:00

abstract：:Mutant Ras proteins are important drivers of human cancers, yet no approved drugs act directly on this difficult target. Over the last decade, the idea has emerged that oncogenic signaling can be diminished by molecules that drive Ras into orientations in which effector-binding interfaces are occluded by the cell memb...

journal_title：Biophysical journal

authors： Neale C,García AE

更新日期：2020-03-10 00:00:00

abstract：:Xenopus melanophores have pigment organelles or melanosomes which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. Melanosomes are transported by microtubule motors, kinesin-2 and cytoplasmic dynein, and an actin motor, myosin-V. We explored the regulation of melanosome trans...

journal_title：Biophysical journal

authors： Levi V,Serpinskaya AS,Gratton E,Gelfand V

更新日期：2006-01-01 00:00:00

abstract：:Ribosomes isolated from growing hyphae of Neurospora crassa contain 53 per cent protein and 47 per cent RNA and have a sedimentation coefficient of 81S at 20 degrees C and infinite dilution. These ribosomes are stable at pH 7.4 in the presence of 0.01 M and 0.002 M MgCl(2) but undergo a dissociation into smaller parti...

journal_title：Biophysical journal

authors： STORCK R

更新日期：1963-01-01 00:00:00

abstract：:Flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD) are essential flavoprotein cofactors. A riboflavin kinase (RFK) activity catalyzes riboflavin phosphorylation to FMN, which can then be transformed into FAD by an FMN:adenylyltransferase (FMNAT) activity. Two enzymes are responsible for each one of thes...

journal_title：Biophysical journal

authors： Lans I,Seco J,Serrano A,Burbano R,Cossio P,Daza MC,Medina M

更新日期：2018-09-18 00:00:00

abstract：:The structure of fully hydrated gel phase dimyristoylphosphatidylcholine lipid bilayers was obtained at 10 degrees C. Oriented lipid multilayers were used to obtain high signal-to-noise intensity data. The chain tilt angle and an estimate of the methylene electron density were obtained from wide angle reflections. The...

journal_title：Biophysical journal

authors： Tristram-Nagle S,Liu Y,Legleiter J,Nagle JF

更新日期：2002-12-01 00:00:00

abstract：:We analyze a two-state stochastic corral model for regulation of protein diffusion in a cell membrane. This model could mimic control of protein transport in the membrane by the cytoskeleton. The dynamic corral acts as a gate which when open permits an otherwise trapped protein to escape to a neighboring corral in the...

journal_title：Biophysical journal

authors： Leitner DM,Brown FL,Wilson KR

更新日期：2000-01-01 00:00:00

abstract：:A complete description of protein structure and function must include a proper treatment of mechanisms that lead to cooperativity. The helix/coil transition serves as a simple example of a cooperative folding process, commonly described by a nucleation-propagation mechanism. The prevalent view is that coil structure m...

journal_title：Biophysical journal

authors： Vorov OK,Livesay DR,Jacobs DJ

更新日期：2009-12-02 00:00:00

abstract：:Here we present a model for maltodextrin translocation through maltoporin channels. In a first step, our theoretical analysis does consider the case of a single binding site for a given substrate in a structurally unaffected channel with a possibly different entrance barrier on either side. It is shown how by means of...

journal_title：Biophysical journal

authors： Schwarz G,Danelon C,Winterhalter M

更新日期：2003-05-01 00:00:00

abstract：:Clostridium difficile (C. diff) is one of the most common and most severe hospital-acquired infections; its consequences range from lengthened hospital stay to outright lethality. C. diff causes cellular damage through the action of two large toxins TcdA and TcdB. Recently, there has been increased effort toward devel...

journal_title：Biophysical journal

authors： Swett RJ,Cisneros GA,Feig AL

更新日期：2013-07-16 00:00:00

abstract：:Several strategies directed toward increasing the participation of minority students in physics and biophysics are presented. Since the number of minority students entering college with an interest in science and mathematics must be increased if we expect to see more students graduating in science, several programs ai...

journal_title：Biophysical journal

authors： Tanaka JC,Gladney LD

更新日期：1993-07-01 00:00:00

abstract：:Thermal inactivation of T(4) and lambda bacteriophage shows two components of differing sensitivity are present. These cannot be interpreted as owing to nucleic acid and protein. One protein function-the inhibition of radiation-induced DNA degradation-is lost with quite different thermal kinetics. lambda heated in the...

journal_title：Biophysical journal

authors： Pollard EC,Solosko W

更新日期：1971-01-01 00:00:00

abstract：:In the photocycle of bacteriorhodopsin (bR) from Halobacterium salinarum mutant L93A, the O-intermediate accumulates and the cycling time is increased approximately 200 times. Nevertheless, under continuous illumination, the protein pumps protons at near wild-type rates. We excited the mutant L93A in purple membrane w...

journal_title：Biophysical journal

authors： Tóth-Boconádi R,Keszthelyi L,Stoeckenius W

更新日期：2003-06-01 00:00:00

abstract：:Pure Michaelis-Menten enzymes have been studied (i.e., enzymes with a hyperbolic (S, V) behavior in a well-stirred solution). When such enzymes are associated with a structure in vitro, even in the simplest conceivable form (immobilization in a homogeneous gel), they can produce enzymic or transport reactions with man...

journal_title：Biophysical journal

authors： Vincent JC,Thellier M

更新日期：1983-01-01 00:00:00

abstract：:The impedance measured in a strip of heart tissue from the moth Hyalophora cecropia is fitted by circuit models of several configurations. The circuits include: (a) a single R-C circuit (b) a double R-C circuit (c) terminated transmission lines, and (d) a pattern of cells with cell-to-cell transmission paths. The last...

journal_title：Biophysical journal

authors： Stibitz GR,McCann FV

更新日期：1974-02-01 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00