Abstract:
:A new stable substrate applicable to the observation of DNA molecules by atomic force microscopy (AFM) was fabricated from a ultrasmooth sapphire (alpha-Al2O3 single crystal) plate. The atomically ultrasmooth sapphire as obtained by high-temperature annealing has hydrophobic surfaces and could not be used for the AFM observation of DNA. However, sapphire treated with Na3PO4 aqueous solution exhibited a hydrophilic character while maintaining a smooth surface structure. The surface of the wet-treated sapphire was found by x-ray photoelectron spectroscopy and AFM to be approximately 0.3 nm. The hydrophilic surface character of the ultrasmooth sapphire plate made it easy for DNA molecules to adhere to the plate. Circular molecules of the plasmid DNA could be imaged by AFM on the hydrophilic ultrasmooth sapphire plate.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Yoshida K,Yoshimoto M,Sasaki K,Ohnishi T,Ushiki T,Hitomi J,Yamamoto S,Sigeno Mdoi
10.1016/S0006-3495(98)77878-6subject
Has Abstractpub_date
1998-04-01 00:00:00pages
1654-7issue
4eissn
0006-3495issn
1542-0086pii
S0006-3495(98)77878-6journal_volume
74pub_type
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