DNA multiphoton absorption generates localized damage for studying repair dynamics in live cells.

abstract：:Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous...

journal_title：Biophysical journal

authors： Bishop J,Chagovetz AM,Blair S

更新日期：2008-03-01 00:00:00

abstract：:The phase transitions of dipalmitoylphosphatidylethanolamine (DPPE) in excess water have been examined by low-angle time-resolved x-ray diffraction and calorimetry at low scan rates. The lamellar subgel/lamellar liquid-crystalline (L(c) --> L(alpha)), lamellar gel/lamellar liquid-crystalline (L(beta) --> L(alpha)), an...

journal_title：Biophysical journal

authors： Yao H,Hatta I,Koynova R,Tenchov B

更新日期：1992-03-01 00:00:00

abstract：:Wiener kernels have been measured for the light growth response of a number of mutants of Phycomyces which show abnormal phototropism (mad mutants). Representative mutants were chosen from the six complementation groups (madA to madF) associated with the light response pathway. One group, madA, associated with the inp...

journal_title：Biophysical journal

authors： Lipson ED

更新日期：1975-10-01 00:00:00

abstract：:Two mechanisms for the interaction of alternating electrical fields with biological tissue are the development of heat, via i(2)R losses, and field-induced force effects, via differences in passive electrical properties. It has been shown that for continuous wave (CW) fields in media of physiologic electrical conducti...

journal_title：Biophysical journal

authors： Sher LD,Kresch E,Schwan HP

更新日期：1970-10-01 00:00:00

abstract：:Profiles of negatively stained gap junctions have been measured by grid sectioning. After normal levels of electron irradiation, the membrane thickness shrinks to about half that of unirradiated controls, but no shrinkage occurs in the hexagonal lattice plane. Even under low irradiation conditions, there is significan...

journal_title：Biophysical journal

authors： Sosinsky GE,Jésior JC,Caspar DL,Goodenough DA

更新日期：1988-05-01 00:00:00

abstract：:The hydrodynamic properties of rigid particles are calculated from models composed of spherical elements (beads) using theories developed by Kirkwood, Bloomfield, and their coworkers. Bead models have usually been built in such a way that the beads fill the volume occupied by the particles. Sometimes the beads are few...

journal_title：Biophysical journal

authors： Carrasco B,García de la Torre J

更新日期：1999-06-01 00:00:00

abstract：:The actin cytoskeleton fulfills numerous key cellular functions, which are tightly regulated in activity, localization, and temporal patterning by actin binding proteins. Tropomyosins and gelsolin are two such filament-regulating proteins. Here, we investigate how the effects of tropomyosins are coupled to the binding...

journal_title：Biophysical journal

authors： Kis-Bicskei N,Bécsi B,Erdődi F,Robinson RC,Bugyi B,Huber T,Nyitrai M,Talián GC

更新日期：2018-02-27 00:00:00

abstract：:The active amplification of sound-induced vibrations in the cochlea, known to be crucial for auditory sensitivity and frequency selectivity, is not well understood. The outer hair cell (OHC) somatic electromotility is a potential mechanism for such amplification. Its effectiveness in vivo is putatively limited by the ...

journal_title：Biophysical journal

authors： Ramamoorthy S,Nuttall AL

更新日期：2012-02-08 00:00:00

abstract：:Five molecular dynamics computer simulations were performed on different phospholipid:sterol membrane systems in order to study the influence of sterol structure on membrane properties. Three of these simulated bilayer systems were composed of a 1:8 sterol:phospholipid ratio, each of which employed one of the sterol m...

journal_title：Biophysical journal

authors： Smondyrev AM,Berkowitz ML

更新日期：2001-04-01 00:00:00

abstract：:The information theory measure of transfer entropy is used to extract the causality of correlated motions from molecular dynamics simulations. For each pair of correlated residues, the method quantifies which residue drives the correlated motions, and which residue responds. The measure reveals how correlated motions ...

journal_title：Biophysical journal

authors： Kamberaj H,van der Vaart A

更新日期：2009-09-16 00:00:00

abstract：:Epithelial injury induces rapid recruitment of antimicrobial leukocytes to the wound site. In zebrafish larvae, activation of the epithelial NADPH oxidase Duox at the wound margin is required early during this response. Before injury, leukocytes are near the vascular region, that is, ∼100-300 μm away from the injury s...

journal_title：Biophysical journal

authors： Jelcic M,Enyedi B,Xavier JB,Niethammer P

更新日期：2017-05-09 00:00:00

abstract：:Values of K, delta G(o), delta H(o), delta S(o) and delta C(po) for the binding reaction of small organic ligands forming 1:1 complexes with either alpha- or beta-cyclodextrin were obtained by titration calorimetry from 15 degrees C to 45 degrees C. A hydrogen bond or hydrophobic interaction was introduced by adding a...

journal_title：Biophysical journal

authors： Ross PD,Rekharsky MV

更新日期：1996-10-01 00:00:00

abstract：:A sample preparation method using spherical glass ampoules has been used to achieve 1.5-Hz resolution in 1H magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of aqueous multilamellar dispersions of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (P...

journal_title：Biophysical journal

authors： Zhou Z,Sayer BG,Hughes DW,Stark RE,Epand RM

更新日期：1999-01-01 00:00:00

abstract：:Deuterium nuclear magnetic resonance spectroscopy was used to study the thermotropic phase behavior of dilauroylphosphatidylcholine (DLPC) bilayers at pressures up to 221 MPa. Pressure was found to separate the liquid crystal to gel transition from the gel to ordered crystalline phase transition. The jump in chain ord...

journal_title：Biophysical journal

authors： Bonev B,Morrow MR

更新日期：1996-06-01 00:00:00

abstract：:Most of the important types of interactions that occur in cells can be characterized as binding-diffusion type processes, and can be quantified by kinetic rate constants such as diffusion coefficients (D) and binding rate constants (k(on) and k(off)). Confocal FRAP is a potentially important tool for the quantitative ...

journal_title：Biophysical journal

authors： Kang M,Day CA,DiBenedetto E,Kenworthy AK

更新日期：2010-11-03 00:00:00

abstract：:At low surface concentrations that permit formation of impermeable membranes, neuronal soluble N-ethyl maleimide sensitive factor attachment protein receptor (SNARE) proteins form a stable, parallel, trans complex when vesicles are brought into contact by a low concentration of poly(ethylene glycol) (PEG). Surprisingl...

journal_title：Biophysical journal

authors： Dennison SM,Bowen ME,Brunger AT,Lentz BR

更新日期：2006-03-01 00:00:00

abstract：:To characterize elastic properties and geometrical parameters of individual, whole myosin molecules during their interaction with actin we sparsely adsorbed myosin molecules to nanometer-sized microspheres. Thermally driven position fluctuations of these microspheres were recorded with the three-dimensional detection ...

journal_title：Biophysical journal

authors： Scholz T,Altmann SM,Antognozzi M,Tischer C,Hörber JK,Brenner B

更新日期：2005-01-01 00:00:00

abstract：:Single-molecule methods provide direct measurements of macromolecular dynamics, but are limited by the number of degrees of freedom that can be followed at one time. High-resolution rotor bead tracking (RBT) measures DNA torque, twist, and extension, and can be used to characterize the structural dynamics of DNA and d...

journal_title：Biophysical journal

authors： Ivanov IE,Lebel P,Oberstrass FC,Starr CH,Parente AC,Ierokomos A,Bryant Z

更新日期：2018-01-23 00:00:00

abstract：:Ubiquinol cytochrome c oxidoreductase (bc1 complex) serves as an important electron junction in many respiratory systems. It funnels electrons coming from NADH and ubiquinol to cytochrome c, but it is also capable of producing significant amounts of the free radical superoxide. In situ and in other experimental system...

journal_title：Biophysical journal

authors： Bazil JN

更新日期：2017-10-03 00:00:00

abstract：:The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+...

journal_title：Biophysical journal

authors： Berdiev BK,Latorre R,Benos DJ,Ismailov II

更新日期：2001-05-01 00:00:00

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:Regulation of the F-actin severing activity of gelsolin by Ca2+ has been investigated under physiologic ionic conditions. Tryptophan fluorescence intensity measurements indicate that gelsolin contains at least two Ca2+ binding sites with affinities of 2.5 x 10(7) M-1 and 1.5 x 10(5) M-1. At F-actin and gelsolin concen...

journal_title：Biophysical journal

authors： Kinosian HJ,Newman J,Lincoln B,Selden LA,Gershman LC,Estes JE

更新日期：1998-12-01 00:00:00

abstract：:We have formulated a kinetic model for the primary steps that occur at the cell membrane during receptor-mediated endocytosis. This model includes the diffusion of receptor molecules, the binding of receptors to coated pits, the loss of coated pits by invagination, and random reinsertion of receptors and coated pits. ...

journal_title：Biophysical journal

authors： Keizer J,Ramirez J,Peacock-López E

更新日期：1985-01-01 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:The fluorescein arsenical hairpin binder (FlAsH) shows much promise to determine the relative orientations of protein regions and structures even in living cells and in the plasma membrane. In this study, we characterized FlAsH's photophysical properties by steady-state anisotropy and time-resolved single photon count...

journal_title：Biophysical journal

authors： Spille JH,Zürn A,Hoffmann C,Lohse MJ,Harms GS

更新日期：2011-02-16 00:00:00

abstract：:Diffraction rings corresponding to the first, second, and third order were obtained by laser light illumination from a suspension of rabbit glycerinated psoas myofibrils (diameter, 1-2 microns; average length of the straight region, 44 microns; average sarcomere length, 2.2-2.6 microns) of which the optical thickness ...

journal_title：Biophysical journal

authors： Ishiwata S,Okamura N

更新日期：1989-12-01 00:00:00

abstract：:The closed-state crystal structure of prokaryotic inward rectifier, KirBac1.1, has implicated four inner helical phenylalanines near the cytoplasmic side as a possible locus of the channel gate. In the present study, we investigate whether this structural feature corresponds to the physiological pH gate of the renal i...

journal_title：Biophysical journal

authors： Sackin H,Nanazashvili M,Palmer LG,Krambis M,Walters DE

更新日期：2005-04-01 00:00:00

abstract：:Choice of network topology for gene regulation has been a question of interest for a long time. How do simple and more complex topologies arise? In this work, we analyze the topology of the marRAB operon in Escherichia coli, which is associated with control of expression of genes associated with conferring resistance ...

journal_title：Biophysical journal

authors： Prajapat MK,Jain K,Saini S

更新日期：2015-10-06 00:00:00

abstract：:The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

journal_title：Biophysical journal

authors： Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

更新日期：2007-09-01 00:00:00

abstract：:Formation of α-helices is a fundamental process in protein folding and assembly. By studying helix formation in molecular simulations of a series of alanine-based peptides, we obtain the temperature-dependent α-helix propensities of all 20 naturally occurring residues with two recent additive force fields, Amber ff03w...

journal_title：Biophysical journal

authors： Best RB,de Sancho D,Mittal J

更新日期：2012-03-21 00:00:00