Arginase of Helicobacter Gastric Pathogens Uses a Unique Set of Non-catalytic Residues for Catalysis.

abstract：:The addition of 10(-3) M p-chloromercuribenzene sulfonate (PCMBS) to the solution bathing the mucosal surface of rabbit colon has no effect on the rate of active Na transport but blocks or reverses the inhibitory action of amiloride. The tissue must be exposed to PCMBS for 20-30 min for a complete blocking effect, and...

journal_title：Biophysical journal

authors： Gottlieb GP,Turnheim K,Frizzell RA,Schultz SG

更新日期：1978-04-01 00:00:00

abstract：:Intensities of x-ray scattering from a series of fragmented rabbit muscle sarcoplasmic reticulum (SR) samples have been measured over the range x = 0.05 to s = 0.25. By varying the relative concentrations of lipid and protein (chiefly the Mg++-dependent, Ca++- stimulated ATPase) in the membranes of this series, and by...

journal_title：Biophysical journal

authors： Brady GW,Fein DB,Harder ME,Spehr R,Meissner G

更新日期：1981-04-01 00:00:00

abstract：:A promising strategy to control the aggregation of the Alzheimer's Aβ peptide in the brain is the clearance of Aβ from the central nervous system into the peripheral blood plasma. Among plasma proteins, human serum albumin plays a critical role in the Aβ clearance to the peripheral sink by binding to Aβ oligomers and ...

journal_title：Biophysical journal

authors： Milojevic J,Melacini G

更新日期：2011-01-05 00:00:00

abstract：:Many experiments have measured the effect of force on the dissociation of single selectin bonds, but it is not yet clear how the force dependence of molecular dissociation can influence the rolling of cells expressing selectin molecules. Recent experiments using constant-force atomic force microscopy or high-resolutio...

journal_title：Biophysical journal

authors： Caputo KE,Lee D,King MR,Hammer DA

更新日期：2007-02-01 00:00:00

abstract：:The average sizes of fluid and gel domains in the two-component, two-phase system formed from mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine were determined from an analysis of the electron spin resonance spectral lineshapes of a dimyristoyl phosphatidylcholine-nitroxide spin label as a...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Thompson TE

更新日期：1992-08-01 00:00:00

abstract：:In this article we present a study of a new class of surfactants denoted as PEG1500-12-acyloxystearates, which have potential use as pharmaceutical solubilizers. These amphiphilic molecules present interesting properties with regard to cell damage effects. PEG1500-12-acyloxystearates with C(14) to C(16) acyloxy chains...

journal_title：Biophysical journal

authors： Vaccaro M,von Corswant C,Söderman O

更新日期：2007-12-15 00:00:00

abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...

journal_title：Biophysical journal

authors： Butler WL

更新日期：1972-07-01 00:00:00

abstract：:The photochemical reaction process of bacteriorhodopsin in the nanosecond time range (-120-860 ns) was measured in the 1400-900 cm-1 region with an improved time resolved dispersive-type infrared spectrometer. The system is equipped with a newly developed detection unit whose instrumental response to a 5-ns laser puls...

journal_title：Biophysical journal

authors： Sasaki J,Yuzawa T,Kandori H,Maeda A,Hamaguchi H

更新日期：1995-05-01 00:00:00

abstract：:We present a three-dimensional tracking routine for nondiffraction-limited particles, which significantly reduces pixel bias. Our technique allows for increased resolution compared to that of previous methods, especially at low magnification or at high signal/noise ratio. This enables tracking with nanometer accuracy ...

journal_title：Biophysical journal

authors： van Loenhout MT,Kerssemakers JW,De Vlaminck I,Dekker C

更新日期：2012-05-16 00:00:00

abstract：:Computational methods that predict three-dimensional structures from amino acid sequences have become increasingly accurate and have provided insights into structure-function relationships for proteins in the absence of structural data. However, the accuracy of computational structural models requires experimental app...

journal_title：Biophysical journal

authors： Tateyama M,Liu H,Yang AS,Cormier JW,Kass RS

更新日期：2004-03-01 00:00:00

abstract：:Model compound studies in the literature show how Hofmeister ion interactions affect protein stability. Although model compound results are typically obtained as salting-out constants, they can be used to find out how the interactions affect protein stability. The null point in the Hofmeister series, which divides pro...

journal_title：Biophysical journal

authors： Baldwin RL

更新日期：1996-10-01 00:00:00

abstract：:There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In additio...

journal_title：Biophysical journal

authors： Jiang Y,Rabbi M,Kim M,Ke C,Lee W,Clark RL,Mieczkowski PA,Marszalek PE

更新日期：2009-02-01 00:00:00

abstract：:The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle relies on the direct i...

journal_title：Biophysical journal

authors： Milhiet PE,Gubellini F,Berquand A,Dosset P,Rigaud JL,Le Grimellec C,Lévy D

更新日期：2006-11-01 00:00:00

abstract：:The molecular cascade that controls switching of the direction of rotation of Escherichia coli flagellar motors is well known, but the conformational changes that allow the rotor to switch are still unclear. The signaling molecule CheY, when phosphorylated, binds to the C-ring at the base of the rotor, raising the pro...

journal_title：Biophysical journal

authors： Hosu BG,Berg HC

更新日期：2018-02-06 00:00:00

abstract：:On the basis of its role in the analysis of mammillary compartmental systems, a matrix with non-zero elements in the first row, first column, and along the main diagonal and with zero elements elsewhere is called a mammillary matrix. It is pointed out that such matrices occur in a variety of biological problems includ...

journal_title：Biophysical journal

authors： Hearon JZ

更新日期：1964-01-01 00:00:00

abstract：:We have studied the atomic level structure of mineralized articular cartilage with heteronuclear solid-state NMR, our aims being to identify the inorganic species present at the surfaces of the mineral crystals which may interact with the surrounding organic matrix and to determine which components of the organic matr...

journal_title：Biophysical journal

authors： Duer MJ,Friscić T,Murray RC,Reid DG,Wise ER

更新日期：2009-04-22 00:00:00

abstract：:Quasilongitudinal sound velocities and the second-order elastic moduli of tetragonal hen egg-white lysozyme crystals were determined as a function of relative humidity (RH) by Brillouin scattering. In hydrated crystals the measured sound velocities in the [110] plane vary between 2.12 +/- 0.03 km/s along the [001] dir...

journal_title：Biophysical journal

authors： Speziale S,Jiang F,Caylor CL,Kriminski S,Zha CS,Thorne RE,Duffy TS

更新日期：2003-11-01 00:00:00

abstract：:The effect of cross-bridge clustering and head-head competition on the mechanical response of skeletal muscle under equilibrium conditions is considered. For this purpose, the recent multiple site equilibrium cross-bridge model of Schoenberg (Schoenberg, M., 1985, Biophys. J., 48:467-475) is extended in accordance wit...

journal_title：Biophysical journal

authors： Tözeren A,Schoenberg M

更新日期：1986-11-01 00:00:00

abstract：:The high gradient magnetic separation technique has been applied to separate paramagnetic erythrocytes from a cell suspension that also contained diamagnetic cells. Paramagnetism was induced in the red blood cells by oxidizing the iron atoms in the cell hemoglobin to the ferric state (methemoglobin). Diamagnetic cells...

journal_title：Biophysical journal

authors： Owen CS

更新日期：1978-05-01 00:00:00

abstract：:Bases, nucleosides, nucleotides, and polynucleotides were exposed to chemically generated singlet oxygen to determine whether the species oxidized paralleled those oxidized in photodynamic reactions. In neutral or basic aqueous solution guanine, guanosine, deoxyguanosine, guanylic acid, deoxyguanylic acid, thymine, an...

journal_title：Biophysical journal

authors： Hallett FR,Hallett BP,Snipes W

更新日期：1970-04-01 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

journal_title：Biophysical journal

authors： Dickinson GD,Parker I

更新日期：2013-01-22 00:00:00

abstract：:We studied the photocurrents of a cyanobacterial rhodopsin Gloeobacter violaceus (GR) in Xenopus laevis oocytes and HEK-293 cells. This protein is a light-driven proton pump with striking similarities to marine proteorhodopsins, including the D121-H87 cluster of the retinal Schiff base counterion and a glutamate at po...

journal_title：Biophysical journal

authors： Vogt A,Wietek J,Hegemann P

更新日期：2013-11-05 00:00:00

abstract：:The morphological features of α-synuclein (AS) amyloid aggregation in vitro and in cells were elucidated at the nanoscale by far-field subdiffraction fluorescence localization microscopy. Labeling AS with rhodamine spiroamide probes allowed us to image AS fibrillar structures by fluorescence stochastic nanoscopy with ...

journal_title：Biophysical journal

authors： Roberti MJ,Fölling J,Celej MS,Bossi M,Jovin TM,Jares-Erijman EA

更新日期：2012-04-04 00:00:00

abstract：:An assumption usually made when developing kinetic models for the gating of ion channels is that the transitions among the various states involved in the gating obey microscopic reversibility. If this assumption is incorrect, then the models and estimated rate constants made with the assumption would be in error. This...

journal_title：Biophysical journal

authors： Song L,Magleby KL

更新日期：1994-07-01 00:00:00

abstract：:The kinetics of aggregation of alpha-synuclein are usually studied by turbidity or Thio-T fluorescence. Here we follow the disappearance of monomers and the formation of early oligomers using fluorescence correlation spectroscopy. Alexa488-labeled A140C-synuclein was used as a fluorescent probe in trace amounts in the...

journal_title：Biophysical journal

authors： Nath S,Meuvis J,Hendrix J,Carl SA,Engelborghs Y

更新日期：2010-04-07 00:00:00

abstract：:This is the first in a series of four papers in which we present the numerical simulation of the application of the voltage clamp technique to excitable cells. In this paper we describe the application of the Crank-Nicolson (1947) method for the solution of the parabolic partial differential equations that describe a ...

journal_title：Biophysical journal

authors： Moore JW,Ramón F,Joyner RW

更新日期：1975-01-01 00:00:00

abstract：:The interplay between epigenetic modification and chromatin compaction is implicated in the regulation of gene expression, and it comprises one of the most fascinating frontiers in cell biology. Although a complete picture is still lacking, it is generally accepted that the differentiation of embryonic stem (ES) cells...

journal_title：Biophysical journal

authors： Chalut KJ,Höpfler M,Lautenschläger F,Boyde L,Chan CJ,Ekpenyong A,Martinez-Arias A,Guck J

更新日期：2012-11-21 00:00:00

abstract：:The motility of Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion...

journal_title：Biophysical journal

authors： Rogers SS,Waigh TA,Lu JR

更新日期：2008-04-15 00:00:00

abstract：:Structural changes in frog skeletal muscle were studied using x-ray diffraction with a time resolution of 0.53-1.02 ms after a single electrical stimulus at 8 degrees C. Tension began to drop at 6 ms (latency relaxation), reached a minimum at 8 ms, and then twitch tension developed. The intensity of the meridional ref...

journal_title：Biophysical journal

authors： Yagi N

更新日期：2003-02-01 00:00:00