High gradient magnetic separation of erythrocytes.

Abstract:

:The high gradient magnetic separation technique has been applied to separate paramagnetic erythrocytes from a cell suspension that also contained diamagnetic cells. Paramagnetism was induced in the red blood cells by oxidizing the iron atoms in the cell hemoglobin to the ferric state (methemoglobin). Diamagnetic cells were either untreated erythrocytes, containing oxyferrohemoglobin, or leukocytes in a suspension of mouse spleen cells. Cell suspensions were passed through a column containing 40 micron diameter stainless steel wire in a high magnetic field (33 kG). The paramagnetic cells were retained on the surface of the wire while the diamagnetic cells passed through. Elution of the paramagnetic cells was accomplished by removing the column from the magnet, in effect turning off the field.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Owen CS

doi

10.1016/S0006-3495(78)85482-4

subject

Has Abstract

pub_date

1978-05-01 00:00:00

pages

171-8

issue

2

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(78)85482-4

journal_volume

22

pub_type

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