Investigation of the adsorption of PEG1500-12-acyloxystearate surfactants onto phospholipid bilayers: an ellipsometry and cryo-TEM study.

abstract：:In recent experimental reports, robust circadian oscillation of the phosphorylation level of KaiC has been reconstituted by incubating three cyanobacterial proteins, KaiA, KaiB, and KaiC, with ATP in vitro. This reconstitution indicates that protein-protein interactions and the associated ATP hydrolysis suffice to gen...

journal_title：Biophysical journal

authors： Nagai T,Terada TP,Sasai M

更新日期：2010-06-02 00:00:00

abstract：:Biological membranes are composed of a large number lipid species differing in hydrophobic length, degree of saturation, and charge and size of the headgroup. We now present data on the effect of hydrocarbon chain length of the lipids and headgroup composition on the lateral mobility of the proteins in model membranes...

journal_title：Biophysical journal

authors： Ramadurai S,Duurkens R,Krasnikov VV,Poolman B

更新日期：2010-09-08 00:00:00

abstract：:A single isolated sinoatrial pacemaker cell presents intrinsic interbeat interval (IBI) variability that is believed to result from the stochastic characteristics of the opening and closing processes of membrane ion channels. To our knowledge, a novel mathematical framework was developed in this work to address the ef...

journal_title：Biophysical journal

authors： Dvir H,Zlochiver S

更新日期：2015-03-10 00:00:00

abstract：:KATP channels assemble from four regulatory SUR1 and four pore-forming Kir6.2 subunits. At the single-channel current level, ATP-dependent gating transitions between the active burst and the inactive interburst conformations underlie inhibition of the KATP channel by intracellular ATP. Previously, we identified a slow...

journal_title：Biophysical journal

authors： Drain P,Geng X,Li L

更新日期：2004-04-01 00:00:00

abstract：:The cell membrane having a transport system is inferred to be flexible when its function is being activated. For the brush border membrane vesicles prepared from rat small intestine, which have the co-transport system of Na+ and glucose, the membrane elasticity was measured as a function of the d-glucose concentration...

journal_title：Biophysical journal

authors： Miyamoto S,Maeda T,Fujime S

更新日期：1988-04-01 00:00:00

abstract：:Previous studies have demonstrated that the carboxyl terminus of the gap junction protein Cx43 (Cx43CT) can act as an independent, regulatory domain that modulates intercellular communication in response to appropriate chemical stimuli. Here, we have used NMR, chemical cross-linking, and analytical ultracentrifugation...

journal_title：Biophysical journal

authors： Sorgen PL,Duffy HS,Spray DC,Delmar M

更新日期：2004-07-01 00:00:00

abstract：:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Prot...

journal_title：Biophysical journal

authors： van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

更新日期：2013-03-05 00:00:00

abstract：:Elastin-derived peptides are released from the extracellular matrix remodeling by numerous proteases and seem to regulate many biological processes, notably cancer progression. The canonical elastin peptide is VGVAPG, which harbors the XGXXPG consensus pattern, allowing interaction with the elastin receptor complex lo...

journal_title：Biophysical journal

authors： Hernández B,Crowet JM,Thiery J,Kruglik SG,Belloy N,Baud S,Dauchez M,Debelle L

更新日期：2020-06-02 00:00:00

abstract：:The effects of lipid chain packing and permeant size and shape on permeability across lipid bilayers have been investigated in gel and liquid crystalline dipalmitoylphosphatidylcholine (DPPC) bilayers by a combined NMR line-broadening/dynamic light scattering method using seven short-chain monocarboxylic acids (formic...

journal_title：Biophysical journal

authors： Xiang TX,Anderson BD

更新日期：1998-12-01 00:00:00

abstract：:We present a simple two-parameter model for surface charge directly associated with ion channels. A spherically symmetric "charged shell" models a distribution of surface charge arrayed about the channel entrance, with a corresponding set of image charges behind the plane of the membrane. The transition between a regi...

journal_title：Biophysical journal

authors： Naranjo D,Latorre R,Cherbavaz D,McGill P,Schumaker MF

更新日期：1994-01-01 00:00:00

abstract：:Interactions between amyloid-β peptide (Aβ) and the cell membrane include interaction with membrane lipids and binding to membrane receptors, both of which are considered to be the toxicity mechanisms of Aβ. However, it is unclear whether both mechanisms lead to cytotoxicity. Thus, we aimed to analyze these two mechan...

journal_title：Biophysical journal

authors： Shi JM,Zhu L,Lan X,Zhao DW,He YJ,Sun ZQ,Wu D,Li HY

更新日期：2020-09-15 00:00:00

abstract：:We have characterized the fluorescence properties of 6-dodecanoyl-2-dimethylamine-naphthalene (LAURDAN) in pure interfaces formed by sphingomyelin and 10 chemically related glycosphingolipids (GSLs).1 The GSLs contain neutral and anionic carbohydrate residues in their oligosaccharide chain. These systems were studied ...

journal_title：Biophysical journal

authors： Bagatolli LA,Gratton E,Fidelio GD

更新日期：1998-07-01 00:00:00

abstract：:The effects of lysolipids on phospholipid layer curvature and bending elasticity were examined using x-ray diffraction and the osmotic stress method. Lysolipids with two different head groups, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and differing hydrocarbon chains were mixed with the hexagonal-for...

journal_title：Biophysical journal

authors： Fuller N,Rand RP

更新日期：2001-07-01 00:00:00

abstract：:The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clampe...

journal_title：Biophysical journal

authors： Oberhauser AF,Monck JR,Fernandez JM

更新日期：1992-03-01 00:00:00

abstract：:The difference in the surface charge distribution between light-adapted and dark-adapted purple membranes was investigated with electric dichroism measurements from approximately pH 5 to pH 11. Purple membrane sheets in solution are oriented in a weak electric field by their permanent dipole moment, which is due to th...

journal_title：Biophysical journal

authors： Otomo J,Ohno K,Takeuchi Y,Ikegami A

更新日期：1986-08-01 00:00:00

abstract：:Sum-frequency vibrational spectroscopy (SFVS) is used to measure the intrinsic rate of lipid flip-flop for 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) in planar-supported lipid bilayers (PSs). Asymmetric P...

journal_title：Biophysical journal

authors： Liu J,Conboy JC

更新日期：2005-10-01 00:00:00

abstract：:We describe a statistical technique, the joint peristimulus time (PST) scatter diagram, for the analysis of data from simultaneously recorded neurons subjected to repeated stimulation. Distinguishable features in the scatter diagram are related to effects of the stimulus on the observed neurons and to functional relat...

journal_title：Biophysical journal

authors： Gerstein GL,Perkel DH

更新日期：1972-05-01 00:00:00

abstract：:Stress fibers are cellular contractile actomyosin machines central to wound healing, shear stress response, and other processes. Contraction mechanisms have been difficult to establish because stress fibers in cultured cells typically exert isometric tension and present little kinetic activity. In a recent study, livi...

journal_title：Biophysical journal

authors： Stachowiak MR,O'Shaughnessy B

更新日期：2009-07-22 00:00:00

abstract：:The persistence length of titin from rabbit skeletal muscles was measured using a combination of static and dynamic light scattering, and neutron small angle scattering. Values of persistence length in the range 9-16 nm were found for titin-II, which corresponds to mainly physiologically inelastic A-band part of the p...

journal_title：Biophysical journal

authors： Di Cola E,Waigh TA,Trinick J,Tskhovrebova L,Houmeida A,Pyckhout-Hintzen W,Dewhurst C

更新日期：2005-06-01 00:00:00

abstract：:The actin cytoskeleton fulfills numerous key cellular functions, which are tightly regulated in activity, localization, and temporal patterning by actin binding proteins. Tropomyosins and gelsolin are two such filament-regulating proteins. Here, we investigate how the effects of tropomyosins are coupled to the binding...

journal_title：Biophysical journal

authors： Kis-Bicskei N,Bécsi B,Erdődi F,Robinson RC,Bugyi B,Huber T,Nyitrai M,Talián GC

更新日期：2018-02-27 00:00:00

abstract：:Conductance noise measurement of the open states of alamethicin transmembrane channels reveals excess noise attributable to cooperative low-frequency molecular dynamics that can generate fluctuations approximately 1 A rms in the effective channel pore radius. Single-channel currents through both persistent and nonpers...

journal_title：Biophysical journal

authors： Mak DO,Webb WW

更新日期：1995-12-01 00:00:00

abstract：:A new method, weighted-ensemble Brownian dynamics, is proposed for the simulation of protein-association reactions and other events whose frequencies of outcomes are constricted by free energy barriers. The method features a weighted ensemble of trajectories in configuration space with energy levels dictating the prop...

journal_title：Biophysical journal

authors： Huber GA,Kim S

更新日期：1996-01-01 00:00:00

abstract：:A promising strategy to control the aggregation of the Alzheimer's Aβ peptide in the brain is the clearance of Aβ from the central nervous system into the peripheral blood plasma. Among plasma proteins, human serum albumin plays a critical role in the Aβ clearance to the peripheral sink by binding to Aβ oligomers and ...

journal_title：Biophysical journal

authors： Milojevic J,Melacini G

更新日期：2011-01-05 00:00:00

abstract：:Ultrasonic relaxation of multilamellar liposomes formed from dipalmitoylphosphatidylcholine was measured near the gel-to-liquid crystal transition by a differential ultrasonic resonator. The relaxation time and strength increased remarkably near the transition temperature, indicating a pseudocritical phenomenon. A qua...

journal_title：Biophysical journal

authors： Mitaku S,Jippo T,Kataoka R

更新日期：1983-05-01 00:00:00

abstract：:It is often assumed that pH(i) is spatially uniform within cells. A double-barreled microperfusion system was used to apply solutions of weak acid (acetic acid, CO(2)) or base (ammonia) to localized regions of an isolated ventricular myocyte (guinea pig). A stable, longitudinal pH(i) gradient (up to 1 pH(i) unit) was ...

journal_title：Biophysical journal

authors： Swietach P,Leem CH,Spitzer KW,Vaughan-Jones RD

更新日期：2005-04-01 00:00:00

abstract：:It has long been recognized that protein dynamical processes occur over a wide temporal range. However, the functionality of this spectrum of events remains unclear. In this work, a generalized noise function analysis is applied to a collection of diverse protein dynamical systems. It is shown that a power law model w...

journal_title：Biophysical journal

authors： Dewey TG,Bann JG

更新日期：1992-08-01 00:00:00

abstract：:Under the assumption that vesicles are the anatomic correlate of quantal release, the forces governing the movement of synaptic vesicles inside neurons are analyzed. Semiquantitative calculations are presented to show that a diffuse layer field penetrates a few Debye lengths into the axoplasm. This field binds tightly...

journal_title：Biophysical journal

authors： Remler MP

更新日期：1973-02-01 00:00:00

abstract：:The theory for determination of the orientation of adsorbed fluorescent molecules using total internal reflection fluorescence, as explained in part I of this series, is illustrated by measurements on adsorbed tetramethylpyridinium porphyrin (H2TMPyP) and porphyrin cytochrome c molecules. The results are encouraging, ...

journal_title：Biophysical journal

authors： Bos MA,Kleijn JM

更新日期：1995-06-01 00:00:00

abstract：:As double-stranded DNA is stretched to its B-form contour length, models of polymer elasticity can describe the dramatic increase in measured force. When the molecule is stretched beyond this contour length, it shows a highly cooperative overstretching transition. We have measured the elasticity and overstretching tra...

journal_title：Biophysical journal

authors： Wenner JR,Williams MC,Rouzina I,Bloomfield VA

更新日期：2002-06-01 00:00:00

abstract：:The fusion of a membrane-bounded vesicle with a target membrane is a key step in intracellular trafficking, exocytosis, and drug delivery. Molecular dynamics simulations have been used to study the fusion of small unilamellar vesicles composed of a dipalmitoyl-phosphatidylcholine (DPPC)/palmitic acid 1:2 mixture in at...

journal_title：Biophysical journal

authors： Knecht V,Marrink SJ

更新日期：2007-06-15 00:00:00