Torsion profiling of proteins using magnetic particles.

Abstract:

:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Protein G-Immunoglobulin G (IgG) and IgG-IgG complexes, sandwiched between a substrate and a superparamagnetic particle, for torques in the range between 0.5 × 10(3) and 5 × 10(3) pN·nm. Both molecular systems show torsional stiffening for increasing rotation angle, but the elastic and inelastic torsion stiffnesses are remarkably different. We interpret the results in terms of the structural properties of the molecules. The torsion profiling technique opens new dimensions for research on biomolecular characterization and for research on bio-nanomechanical structure-function relationships.

journal_name

Biophys J

journal_title

Biophysical journal

authors

van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

doi

10.1016/j.bpj.2013.01.027

subject

Has Abstract

pub_date

2013-03-05 00:00:00

pages

1073-80

issue

5

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(13)00132-X

journal_volume

104

pub_type

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