Resolution of multiphasic reactions by the combination of fluorescence total-intensity and anisotropy stopped-flow kinetic experiments.

abstract：:The naturally occurring amino acid cysteine has often been implicated with a crucial role in maintaining protein structure and stability. An intriguing duality in the intrinsic hydrophobicity of the cysteine side chain is that it exhibits both polar as well as hydrophobic characteristics. Here, we have utilized a cyst...

journal_title：Biophysical journal

authors： Iyer BR,Mahalakshmi R

更新日期：2019-07-09 00:00:00

abstract：:The distribution of a selection of cationic fluorescent dyes can be used to measure the membrane potential of individual cells with a microfluorometer. The essential attributes of these dyes include membrane permeability, low membrane binding, spectral properties which are insensitive to environment, and, of course, s...

journal_title：Biophysical journal

authors： Ehrenberg B,Montana V,Wei MD,Wuskell JP,Loew LM

更新日期：1988-05-01 00:00:00

abstract：:The effects of ultraviolet light (UV) irradiation on the rate of DNA replication in synchronized Chinese hamster ovary (CHO) cells were investigated. A technique for measuring semiconservative DNA replication was employed that involved growing the cells in medium containing 5-bromodeoxyuridine and subsequently determi...

journal_title：Biophysical journal

authors： Meyn RE,Hewitt RR,Thomas LF,Humphrey RM

更新日期：1976-05-01 00:00:00

abstract：:The mechanosensitive channel of small conductance (MscS) is a bacterial mechanosensitive channel that opens in response to rapid hypoosmotic stress. Since MscS can be opened solely by membrane stretch without help from any accessory protein, the lipid-protein interface must play a crucial role in sensing membrane tens...

journal_title：Biophysical journal

authors： Nomura T,Sokabe M,Yoshimura K

更新日期：2006-10-15 00:00:00

abstract：:Fluorescent proteins are often used as reporters of transcriptional activity. Here we present a mathematical characterization of a novel fluorescent reporter that was recently engineered to have a short half-life (approximately 12 min). The advantage of this destabilized protein is that it can track the transient tran...

journal_title：Biophysical journal

authors： Wang X,Errede B,Elston TC

更新日期：2008-03-15 00:00:00

abstract：:Cell motility is essential in a variety of biological phenomena ranging from early development to organ homeostasis and diseases. This phenomenon has mainly been studied and characterized on flat surfaces in vitro, whereas such conditions are rarely observed in vivo. Recently, cell motion in three-dimensional microfab...

journal_title：Biophysical journal

authors： Le Maout E,Lo Vecchio S,Kumar Korla P,Jinn-Chyuan Sheu J,Riveline D

更新日期：2020-10-06 00:00:00

abstract：:A sequence determinant of reduction potentials is reported for bacterial [4Fe-4S]-type ferredoxins. The residue that is four residues C-terminal to the fourth ligand of either cluster is generally an alanine or a cysteine. In five experimental ferredoxin structures, the cysteine has the same structural orientation rel...

journal_title：Biophysical journal

authors： Beck BW,Xie Q,Ichiye T

更新日期：2001-08-01 00:00:00

abstract：:The mathematical model of steady-state iron kinetics in the human presented here extends the use of radioactive tracer observations in the determination of erythron maturative and proliferative behavior. Functions descriptive of that behavior appear explicitly in the model together with functions customarily measured ...

journal_title：Biophysical journal

authors： Nooney GC

更新日期：1965-11-01 00:00:00

abstract：:Plasmodium falciparum malaria-infected red blood cells (IRBCs), or erythrocytes, avoid splenic clearance by adhering to host endothelium. Upregulation of endothelial receptors intercellular adhesion molecule-1 (ICAM-1) and cluster of differentiation 36 (CD36) are associated with severe disease pathology. Most in vitro...

journal_title：Biophysical journal

authors： Lim YB,Thingna J,Kong F,Dao M,Cao J,Lim CT

更新日期：2020-01-07 00:00:00

abstract：:Small RNA-mRNA binding is an essential step in RNA interference, an important cellular regulatory process. Calculations of binding free energy have been used in binding site prediction, but the cost of stretching the mRNA loop when the small RNA-mRNA duplex forms requires further exploration. Here, using both polymer ...

journal_title：Biophysical journal

authors： Meng Y,Aalberts DP

更新日期：2013-01-22 00:00:00

abstract：:We examined the effects of brief current pulses on the pacemaker oscillations of the Purkinje fiber using the model of McAllister , Noble, and Tsien (1975. J. Physiol. [Lond.]. 251:1-57). This model was used to construct phase-response curves for brief electric stimuli to find "black holes," where rhythmic activity of...

journal_title：Biophysical journal

authors： Chay TR,Lee YS

更新日期：1984-04-01 00:00:00

abstract：:Molecular dynamics simulations supplement single-molecule pulling experiments by providing the possibility of examining the full free energy landscape using many coordinates. Here, we use an all-atom structure-based model to study the force and temperature dependence of the unfolding of the protein filamin by applying...

journal_title：Biophysical journal

authors： Sun L,Noel JK,Sulkowska JI,Levine H,Onuchic JN

更新日期：2014-12-16 00:00:00

abstract：:The freshwater polyp Hydra can regenerate from tissue fragments or random cell aggregates. We show that the axis-defining step ("symmetry breaking") of regeneration requires mechanical inflation-collapse oscillations of the initial cell ball. We present experimental evidence that axis definition is retarded if these o...

journal_title：Biophysical journal

authors： Soriano J,Rüdiger S,Pullarkat P,Ott A

更新日期：2009-02-18 00:00:00

abstract：:Potassium accumulation associated with outward membrane potassium current was investigated experimentally in myelinated fibers and analyzed in terms of two models-three-compartment and diffusion in an unstirred layer. In the myelinated fibers, as in squid giant axons, the three-compartment model satisfactorily describ...

journal_title：Biophysical journal

authors： Moran N,Palti Y,Levitan E,Stämpfli R

更新日期：1980-12-01 00:00:00

abstract：:Two monocysteine mutants of cardiac muscle troponin C, cTnC(C35S) and cTnC(C84S), were genetically generated and labeled with the fluorescent probe 2-[4'-(iodoacetamido)anilino]naphthalene-6-sulfonic acid (IAANS) at Cys-84 and Cys-35, respectively. Cys-84 is located on helix D in the regulatory N-domain, and Cys-35 is...

journal_title：Biophysical journal

authors： Dong WJ,Wang CK,Gordon AM,Cheung HC

更新日期：1997-02-01 00:00:00

abstract：:Chlamydomonas exhibits a sequence of a photoreceptor current and two flagellar currents upon stimulation with bright green flashes. The currents are thought to be a prerequisite for the well-known photophobic responses. In the preceding paper, we analyzed the kinetics of these currents and their dependence on extracel...

journal_title：Biophysical journal

authors： Nonnengässer C,Holland EM,Harz H,Hegemann P

更新日期：1996-02-01 00:00:00

abstract：:Helix-coil transitions in polyalanine molecules of length 10 are studied by multi-canonical Monte Carlo simulations. The solvation effects are included by either a distance-dependent dielectric permittivity or by a term that is proportional to the solvent-accessible surface area of the peptide. We found a strong depen...

journal_title：Biophysical journal

authors： Peng Y,Hansmann UH

更新日期：2002-06-01 00:00:00

abstract：:The effects of rigor and cycling cross-bridges on distributions of calcium (Ca) bound within sarcomeres of rabbit psoas muscle fibers were compared using electron probe x-ray microanalysis. Calcium in the overlap region of rigor fibers, after correction for that bound to thick filaments, was significantly higher than ...

journal_title：Biophysical journal

authors： Cantino ME,Quintanilla A

更新日期：2007-01-15 00:00:00

abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

journal_title：Biophysical journal

authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

更新日期：2010-10-20 00:00:00

abstract：:The brain Na+ channel-1 (BNC1, also known as MDEG1 or ASIC2) is a member of the DEG/ENaC cation channel family. Mutation of a specific residue (Gly430) that lies N-terminal to the second membrane-spanning domain activates BNC1 and converts it from a Na+-selective channel to one permeable to both Na+ and K+. Because al...

journal_title：Biophysical journal

authors： Adams CM,Price MP,Snyder PM,Welsh MJ

更新日期：1999-03-01 00:00:00

abstract：:Morphogenesis of epithelial tissues requires tight spatiotemporal coordination of cell shape changes. In vivo, many tissue-scale shape changes are driven by pulsatile contractions of intercellular junctions, which are rectified to produce irreversible deformations. The functional role of this pulsatory ratchet and its...

journal_title：Biophysical journal

authors： Staddon MF,Cavanaugh KE,Munro EM,Gardel ML,Banerjee S

更新日期：2019-11-05 00:00:00

abstract：:The subgel phases of unlabeled, specifically chain perdeuterated and specifically 13C = O labeled representative samples of the n-saturated diacylphosphatidylcholines were studied by Fourier-transform infrared spectroscopy. Our results indicate that the spectroscopic properties exhibited by the subgel phases of the lo...

journal_title：Biophysical journal

authors： Lewis RN,McElhaney RN

更新日期：1992-01-01 00:00:00

abstract：:The CLC family of chloride channels and transporters is a functionally diverse group of proteins important in a wide range of physiological processes. ClC-4 and ClC-5 are localized to endosomes and seem to play roles in the acidification of these compartments. These proteins were recently shown to function as Cl(-)/H(...

journal_title：Biophysical journal

authors： Osteen JD,Mindell JA

更新日期：2008-11-15 00:00:00

abstract：:The lipid matrix present in the uppermost layer of the skin, the stratum corneum, plays a crucial role in the skin barrier function. The lipids are organized into two lamellar phases. To gain more insight into the molecular organization of one of these lamellar phases, we performed neutron diffraction studies. In the ...

journal_title：Biophysical journal

authors： Groen D,Gooris GS,Barlow DJ,Lawrence MJ,van Mechelen JB,Demé B,Bouwstra JA

更新日期：2011-03-16 00:00:00

abstract：:The vibrational spectroscopy and population dynamics of excited singlet (2(1)Ag), excited triplet (3B u), and the ground (1Ag) electronic states of carotenoids in chromatophores of Chromatium vinosum (mainly spirilloxanthin and rhodopin) and of the same carotenoids in benzene solutions are examined by picosecond time-...

journal_title：Biophysical journal

authors： Hayashi H,Noguchi T,Tasumi M,Atkinson GH

更新日期：1991-07-01 00:00:00

abstract：:EmrE is a small multidrug resistance transporter that has been well studied as a model for secondary active transport. Because transport requires the protein to convert between at least two states open to opposite sides of the membrane, it is expected that blocking these conformational transitions will prevent transpo...

journal_title：Biophysical journal

authors： Dutta S,Morrison EA,Henzler-Wildman KA

更新日期：2014-08-05 00:00:00

abstract：:We present a minimal model of plasma membrane heterogeneity that combines criticality with connectivity to cortical cytoskeleton. The development of this model was motivated by recent observations of micron-sized critical fluctuations in plasma membrane vesicles that are detached from their cortical cytoskeleton. We i...

journal_title：Biophysical journal

authors： Machta BB,Papanikolaou S,Sethna JP,Veatch SL

更新日期：2011-04-06 00:00:00

abstract：:A recent theory for the long time dynamics of flexible chain molecules is applied for the first time to a peptide of biological importance, the neurotransmitter met-enkephalin. The dynamics of met-enkephalin is considerably more complicated than that of the previously studied glycine oligomers; met-enkephalin contains...

journal_title：Biophysical journal

authors： Kostov KS,Freed KF

更新日期：1999-01-01 00:00:00

abstract：:Forces generated by microtubule polymerization and depolymerization are important for the biological functioning of cells. The mean growth velocity, V, under an opposing force, F, has been measured by; Science 278:856-860) for single microtubules growing in vitro, but their analysis of the data suggested that V decrea...

journal_title：Biophysical journal

authors： Kolomeisky AB,Fisher ME

更新日期：2001-01-01 00:00:00

abstract：:The effect of uncharged, dipolar phloretin on anion and cation conductance through a black lipid membrane can be used to study its adsorption behavior. The adsorption of phloretin can be described by a Langmuir isotherm with weak dipole-dipole interaction. ...

journal_title：Biophysical journal

authors： de Levie R,Rangarajan SK,Seelig PF,Andersen OS

更新日期：1979-02-01 00:00:00