Temperature-Induced Catch-Slip to Slip Bond Transit in Plasmodium falciparum-Infected Erythrocytes.

abstract：:The electrophoretic behavior of defined DNA and RNA oligonucleotide duplexes from 10 to 20 bp in length has been investigated as a function of salt conditions, gel concentration, and temperature. The RNA oligomers migrated much more slowly than the DNA oligomers of the same sequence under all conditions. From sediment...

journal_title：Biophysical journal

authors： Bonifacio GF,Brown T,Conn GL,Lane AN

更新日期：1997-09-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was used to monitor secondary structural changes associated with binding of bovine prothrombin and prothrombin fragment 1 to acidic lipid membranes. Prothrombin and prothrombin fragment 1 were examined under four different conditions: in the presence of (a) Na2EDTA, (b) 5...

journal_title：Biophysical journal

authors： Wu JR,Lentz BR

更新日期：1991-07-01 00:00:00

abstract：:A cell plated on a two-dimensional substrate forms adhesions with that surface. These adhesions, which consist of aggregates of various proteins, are thought to be important in mechanosensation, the process by which the cell senses and responds to the mechanical properties of the substrate (e.g., stiffness). On the ba...

journal_title：Biophysical journal

authors： Walcott S,Kim DH,Wirtz D,Sun SX

更新日期：2011-12-21 00:00:00

abstract：:Extracellular vesicles (EVs) are proposed to play important roles in intercellular communication. Two classes of EVs can be distinguished based on their intracellular origin. Exosomes are generated within endosomes and released when these fuse with the plasma membrane, whereas ectosomes bud directly from the plasma me...

journal_title：Biophysical journal

authors： Jackson CE,Scruggs BS,Schaffer JE,Hanson PI

更新日期：2017-09-19 00:00:00

abstract：:The near-infrared charge transfer band at 760 nm (band III) has been investigated in deoxy and photodissociated dimeric Scapharca hemoglobin. At 300 K, the 10-ns spectrum of the carbonmonoxy derivative photoproduct is shifted by about 6 nm toward longer wavelengths with respect to the deoxy spectrum, both in buffer an...

journal_title：Biophysical journal

authors： Huang J,Leone M,Boffi A,Friedman JM,Chiancone E

更新日期：1996-06-01 00:00:00

abstract：:Membrane fusion proceeds via formation of intermediate nonbilayer structures. The stalk model of fusion intermediate is commonly recognized to account for the major phenomenology of the fusion process. However, in its current form, the stalk model poses a challenge. On one hand, it is able to describe qualitatively th...

journal_title：Biophysical journal

authors： Kozlovsky Y,Kozlov MM

更新日期：2002-02-01 00:00:00

abstract：:Receptor activity modifying proteins (RAMPs) are a family of single-pass transmembrane proteins that dimerize with G-protein-coupled receptors. They may alter the ligand recognition properties of the receptors (particularly for the calcitonin receptor-like receptor, CLR). Very little structural information is availabl...

journal_title：Biophysical journal

authors： Simms J,Hay DL,Wheatley M,Poyner DR

更新日期：2006-07-15 00:00:00

abstract：:The antifungal polyene antibiotic amphotericin B forms channels in lipid membranes that are permeable to ions, water, and nonelectrolytes. Anion, cation, and ion pair coordination in the water-filled pore of the "barrel" unit of the channels was studied by molecular dynamics simulations. Unlike the case of the gramici...

journal_title：Biophysical journal

authors： Khutorsky V

更新日期：1996-12-01 00:00:00

abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....

journal_title：Biophysical journal

authors： Wei P,Zheng BK,Guo PR,Kawakami T,Luo SZ

更新日期：2013-04-02 00:00:00

abstract：:Intracellular Ca2+ was determined with the fura-2 technique during electrically induced contractions in the rabbit rectococcygeus smooth muscle at 22 degreesC. The muscles were electrically activated to give short, reproducible contractions. Intracellular [Ca2+] increased during activation; the increase in [Ca2+] prec...

journal_title：Biophysical journal

authors： Arner A,Malmqvist U,Rigler R

更新日期：1998-10-01 00:00:00

abstract：:Here we model the Alzheimer beta-peptide ion channel with the goal of obtaining insight into the mechanism of amyloid toxicity. The models are built based on NMR data of the oligomers, with the universal U-shaped (strand-turn-strand) motif. After 30-ns simulations in the bilayer, the channel dimensions, shapes and sub...

journal_title：Biophysical journal

authors： Jang H,Zheng J,Nussinov R

更新日期：2007-09-15 00:00:00

abstract：:Calmodulin (CaM) expression is upregulated upon HIV-1 infection and interacts with proteins involved in viral processing, including the multifunctional HIV-1 MA protein. We present here the results of studies utilizing small-angle neutron scattering with contrast variation that, when considered in the light of earlier...

journal_title：Biophysical journal

authors： Taylor JE,Chow JYH,Jeffries CM,Kwan AH,Duff AP,Hamilton WA,Trewhella J

更新日期：2012-08-08 00:00:00

abstract：:The conformation of the polypeptide melittin in lipid membranes as determined by Raman spectroscopy is a bent alpha-helix formed by the mainly hydrophobic residues 1-21, and a nonhelical COOH-terminal segment of the hydrophilic residues 22-26. Fluorescence quenching experiments on residue Trp19 reveal that all COOH-te...

journal_title：Biophysical journal

authors： Vogel H,Jähnig F

更新日期：1986-10-01 00:00:00

abstract：:Microstructured surfaces provide a unique framework to probe cell migration and cytoskeletal dynamics in a standardized manner. Here, we report on the steady-state occupancy probability of cells in asymmetric two-state microstructures that consist of two fibronectin-coated adhesion sites connected by a thin guidance c...

journal_title：Biophysical journal

authors： Fink A,Brückner DB,Schreiber C,Röttgermann PJF,Broedersz CP,Rädler JO

更新日期：2020-02-04 00:00:00

abstract：:A nitroxide spin label attached to the C-terminus of the channel forming peptide alamethicin produces an enhancement of the nuclear spin-lattice relaxation rates of peptide protons as a result of both intermolecular and intramolecular magnetic dipole-dipole interactions. The intermolecular contribution provides eviden...

journal_title：Biophysical journal

authors： North CL,Franklin JC,Bryant RG,Cafiso DS

更新日期：1994-11-01 00:00:00

abstract：:We study the lateral headgroup interactions among phosphatidylcholine (PC) molecules and among phosphatidylethanolamine (PE) molecules in monolayers and extend our previous models. In this paper, we present an extensive set of pressure-area isotherms and surface potential experiments on monolayers of phospholipids ran...

journal_title：Biophysical journal

authors： Mingins J,Stigter D,Dill KA

更新日期：1992-06-01 00:00:00

abstract：:The time correlation function of the shape fluctuations of large (greater than 10 micron), cylindrical, hydrated, phospholipid-membrane vesicles consisting of one bimolecular layer was measured. The restoring force of the membrane was due to the excess curvature of a membrane element. A value for the curvature elastic...

journal_title：Biophysical journal

authors： Schneider MB,Jenkins JT,Webb WW

更新日期：1984-05-01 00:00:00

abstract：:Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

journal_title：Biophysical journal

authors： Grossman SH

更新日期：1991-03-01 00:00:00

abstract：:The pore-forming alpha 1 subunit of L-type calcium (Ca2+) channels is the molecular target of Ca2+ channel blockers such as phenylalkylamines (PAAs). Association and dissociation rates of (-)devapamil were compared for a highly PAA-sensitive L-type Ca2+ channel chimera (Lh) and various class A Ca2+ channel mutants. Th...

journal_title：Biophysical journal

authors： Degtiar VE,Aczél S,Döring F,Timin EN,Berjukow S,Kimball D,Mitterdorfer J,Hering S

更新日期：1997-07-01 00:00:00

abstract：:Calculations are reported of the time-dependent Nernst-Planck equations for a thin permeable membrane between electrolytic solutions. Charge neutrality is assumed for the time-dependent case. The response of such a membrane system to step current input is measured in terms of the time and space changes in concentratio...

journal_title：Biophysical journal

authors： COHEN H,COOLEY JW

更新日期：1965-03-01 00:00:00

abstract：:A detailed picture of water and ion properties in small pores is important for understanding the behavior of biological ion channels. Several recent modeling studies have shown that small, hydrophobic pores exclude water and ions even if they are physically large enough to accommodate them, a mechanism called hydropho...

journal_title：Biophysical journal

authors： Spronk SA,Elmore DE,Dougherty DA

更新日期：2006-05-15 00:00:00

abstract：:We have monitored the organization and dynamics of the hemolytic peptide melittin in membranes containing cholesterol by utilizing the intrinsic fluorescence properties of its functionally important sole tryptophan residue and circular dichroism spectroscopy. The significance of this study is based on the fact that th...

journal_title：Biophysical journal

authors： Raghuraman H,Chattopadhyay A

更新日期：2004-10-01 00:00:00

abstract：:A profound remodeling of the extracellular matrix occurs in many epithelial cancers. In ovarian cancer, the minor collagen isoform of Col III becomes upregulated in invasive disease. Here we use second harmonic generation (SHG) imaging microscopy to probe structural differences in fibrillar models of the ovarian strom...

journal_title：Biophysical journal

authors： Tilbury K,Lien CH,Chen SJ,Campagnola PJ

更新日期：2014-01-21 00:00:00

abstract：:The capability to image single microbial cell surfaces at nanometer scale under native conditions would profoundly impact mechanistic and structural studies of pathogenesis, immunobiology, environmental resistance, and biotransformation. Here, using in vitro atomic force microscopy, we have directly visualized high-re...

journal_title：Biophysical journal

authors： Plomp M,Leighton TJ,Wheeler KE,Malkin AJ

更新日期：2005-01-01 00:00:00

abstract：:Electrical bursting oscillations of mammalian pancreatic beta-cells are synchronous among cells within an islet. While electrical coupling among cells via gap junctions has been demonstrated, its extent and topology are unclear. The beta-cells also share an extracellular compartment in which oscillations of K+ concent...

journal_title：Biophysical journal

authors： Stokes CL,Rinzel J

更新日期：1993-08-01 00:00:00

abstract：:A model is presented for the steric interaction between a plasma membrane protein and the membrane cytoskeleton in the human erythrocyte. The cytoskeleton is treated as a network of polymer chains attached to a flat bilayer, and the membrane protein is a hemisphere of effective radius R(e) with center on the bilayer e...

journal_title：Biophysical journal

authors： Boal DH,Boey SK

更新日期：1995-08-01 00:00:00

abstract：:A new flow configuration allows a flow cytometer of high resolution and stability to be assembled from an inverted fluorescence microscope with incident illumination, a pulse photometer, and a multichannel pulse height analyzer. A nozzle produces a hydrodynamically focused sample stream in a liquid jet that id directe...

journal_title：Biophysical journal

authors： Lindmo T,Steen HB

更新日期：1979-10-01 00:00:00

abstract：:In contrast with most inhalational anesthetics, the anesthetic gases xenon (Xe) and nitrous oxide (N(2)O) act by blocking the N-methyl-d-aspartate (NMDA) receptor. Using x-ray crystallography, we examined the binding characteristics of these two gases on two soluble proteins as structural models: urate oxidase, which ...

journal_title：Biophysical journal

authors： Colloc'h N,Sopkova-de Oliveira Santos J,Retailleau P,Vivarès D,Bonneté F,Langlois d'Estainto B,Gallois B,Brisson A,Risso JJ,Lemaire M,Prangé T,Abraini JH

更新日期：2007-01-01 00:00:00

abstract：:Robust positioning of cells in a tissue against unavoidable noises is important for achieving normal and reproducible morphogenesis. The position in a tissue is represented by morphogen concentrations, and cells read them to recognize their spatial coordinates. From the engineering viewpoint, these positioning process...

journal_title：Biophysical journal

authors： Morishita Y,Iwasa Y

更新日期：2011-11-16 00:00:00

abstract：:Historically, predicting macroscopic blood flow characteristics such as viscosity has been an empirical process due to the difficulty in rigorously including the particulate nature of blood in a mathematical representation of blood rheology. Using a two-dimensional lattice Boltzmann approach, we have simulated the flo...

journal_title：Biophysical journal

authors： Sun C,Munn LL

更新日期：2005-03-01 00:00:00