Cooperative effects of rigor and cycling cross-bridges on calcium binding to troponin C.

abstract：:The spermine-induced DNA condensation is a first-order phase transition. Here, we apply a novel technique fluorescence lifetime correlation spectroscopy to analyze this transition in a greater detail. We show that the method allows for the observation of the condensed and uncondensed molecules simultaneously based sol...

journal_title：Biophysical journal

authors： Humpolícková J,Benda A,Sýkora J,Machán R,Kral T,Gasinska B,Enderlein J,Hof M

更新日期：2008-02-01 00:00:00

abstract：:Using CD spectroscopy, guanine tetraplex formation was studied with short DNA fragments in which cytosine residues were systematically added to runs of guanine either at the 5' or 3' ends. Potassium cations induced the G-tetraplex more easily with fragments having the guanine run at the 5' end, which is just an opposi...

journal_title：Biophysical journal

authors： Penázová H,Vorlicková M

更新日期：1997-10-01 00:00:00

abstract：:Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here, we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay with multicolor single-molecule fluorescence. We demons...

journal_title：Biophysical journal

authors： Severins I,Szczepaniak M,Joo C

更新日期：2018-09-18 00:00:00

abstract：:Thermodynamically rigorous free energy methods in principle allow the exact computation of binding free energies in biological systems. Here, we use thermodynamic integration together with molecular dynamics simulations of a DNA-protein complex to compute relative binding free energies of a series of mutants of a prot...

journal_title：Biophysical journal

authors： Beierlein FR,Kneale GG,Clark T

更新日期：2011-09-07 00:00:00

abstract：:Adenylate kinase, an enzyme that catalyzes the phosphoryl transfer between ATP and AMP, can interconvert between the open and catalytically potent (closed) forms even without binding ligands. Several aspects of the enzyme elasticity and internal dynamics are analyzed here by atomistic molecular dynamics simulations co...

journal_title：Biophysical journal

authors： Pontiggia F,Zen A,Micheletti C

更新日期：2008-12-15 00:00:00

abstract：:We examined the kinetics of voltage-dependent sodium currents in cerebellar Purkinje neurons using whole-cell recording from dissociated neurons. Unlike sodium currents in other cells, recovery from inactivation in Purkinje neurons is accompanied by a sizeable ionic current. Additionally, the extent and speed of recov...

journal_title：Biophysical journal

authors： Raman IM,Bean BP

更新日期：2001-02-01 00:00:00

abstract：:The harmonic mode analysis method was used to characterize the conformational deformability of regular Watson-Crick paired, mismatch- and bulge-containing RNA. Good agreement between atomic Debye-Waller factors derived from x-ray crystallography of a regular RNA oligonucleotide and calculated atomic fluctuations was o...

journal_title：Biophysical journal

authors： Zacharias M,Sklenar H

更新日期：2000-05-01 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:The bile acid-sensitive ion channel is activated by amphiphilic substances such as bile acids or artificial detergents via membrane alterations; however, the mechanism of membrane sensitivity of the bile acid-sensitive ion channel is not known. It has also not been systematically investigated whether other members of ...

journal_title：Biophysical journal

authors： Schmidt A,Alsop RJ,Rimal R,Lenzig P,Joussen S,Gervasi NN,Khondker A,Gründer S,Rheinstädter MC,Wiemuth D

更新日期：2018-03-27 00:00:00

abstract：:A novel way to model permeation through ionic channels is formulated. Our method does not require that equilibrium exists in the channel or at the channel interfaces. In addition, the potential profile does not need to be specified and the assumption of constant field across the membrane does not need to be made. Our ...

journal_title：Biophysical journal

authors： Skinner FK,Ward CA,Bardakjian BL

更新日期：1993-08-01 00:00:00

abstract：:ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

journal_title：Biophysical journal

authors： Cherepanov DA,Junge W

更新日期：2001-09-01 00:00:00

abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

journal_title：Biophysical journal

authors： Lee AI,Brody JP

更新日期：2005-06-01 00:00:00

abstract：:Fluorescence cross-correlation spectroscopy (FCCS) is used to determine interactions and dissociation constants (K(d)s) of biomolecules. The determination of a K(d) depends on the accurate measurement of the auto- and cross-correlation function (ACF and CCF) amplitudes. In the case of complete binding, the ratio of th...

journal_title：Biophysical journal

authors： Foo YH,Naredi-Rainer N,Lamb DC,Ahmed S,Wohland T

更新日期：2012-03-07 00:00:00

abstract：:Five AHCAPs exhibiting a broad-spectrum of antimicrobial activity, were examined with regard to their action in lipid mixtures with two anionic lipids, PG and CL. We find that all of the peptides studied were capable of promoting the formation of crystalline phases of DMPG in mixtures of DMPG and CL, without prior inc...

journal_title：Biophysical journal

authors： Epand RF,Maloy L,Ramamoorthy A,Epand RM

更新日期：2010-06-02 00:00:00

abstract：:Macromolecular transport between the nucleus and cytoplasm occurs through the nuclear pore complexes (NPCs). The NPC in the budding yeast Saccharomyces cerevisiae is a 60-MDa structure embedded in the nuclear envelope and composed of ~30 proteins, termed nucleoporins or nups. Here we present a large-scale analysis of ...

journal_title：Biophysical journal

authors： Damelin M,Silver PA

更新日期：2002-12-01 00:00:00

abstract：:By employing classical molecular dynamics, correlation analysis of coupling between slow and fast dynamical modes, and free energy (umbrella) sampling using classical as well as mixed quantum mechanics molecular mechanics force fields, we uncover a possible pathway for phosphoryl transfer in the self-cleaving reaction...

journal_title：Biophysical journal

authors： Radhakrishnan R

更新日期：2007-10-01 00:00:00

abstract：:The thermal stability of a Schizolobium parahyba chymotrypsin inhibitor (SPCI) as a function of pH has been investigated using fluorescence, circular dichroism, and differential scanning calorimetry (DSC). The thermodynamic parameters derived from all methods are remarkably similar and strongly suggest the high stabil...

journal_title：Biophysical journal

authors： Teles RC,Calderon Lde A,Medrano FJ,Barbosa JA,Guimarães BG,Santoro MM,de Freitas SM

更新日期：2005-05-01 00:00:00

abstract：:KATP channels assemble from four regulatory SUR1 and four pore-forming Kir6.2 subunits. At the single-channel current level, ATP-dependent gating transitions between the active burst and the inactive interburst conformations underlie inhibition of the KATP channel by intracellular ATP. Previously, we identified a slow...

journal_title：Biophysical journal

authors： Drain P,Geng X,Li L

更新日期：2004-04-01 00:00:00

abstract：:F-actin, a helical polymer formed by polymerization of the monomers (G-actin), plays crucial roles in various aspects of cell motility. Flexibility of F-actin has been suggested to be important for such a variety of functions. Understanding the flexibility of F-actin requires characterization of a hierarchy of dynamic...

journal_title：Biophysical journal

authors： Fujiwara S,Plazanet M,Matsumoto F,Oda T

更新日期：2008-06-01 00:00:00

abstract：:Cellular plasma membranes have domains that are defined, in most cases, by cytoskeletal elements. The outer half of the bilayer may also contain domains that organize glycosylphosphatidylinositol (GPI)-linked proteins. To define outer membrane barriers, we measured the resistive force on membrane bound beads as they w...

journal_title：Biophysical journal

authors： Suzuki K,Sterba RE,Sheetz MP

更新日期：2000-07-01 00:00:00

abstract：:Phosphorescence spectroscopy on mouse myeloma IgA J539 in rigid solution at 77K revealed the type of anomalous short-lived component in the tryptophan decay originally observed with lysozyme (Churchich, J.E., 1966. Biochim. Biophys. Acta. 120:406-412) and seen in a large number of Bence Jones proteins (Longworth, J.W....

journal_title：Biophysical journal

authors： Li Z,Galley WC

更新日期：1989-08-01 00:00:00

abstract：:The (31)P-nuclear magnetic resonance chemical shift of phosphatidic acid in a membrane is sensitive to the lipid head group packing and can report qualitatively on membrane lateral compression near the aqueous interface. We have used high-resolution (31)P-nuclear magnetic resonance to evaluate the lateral compression ...

journal_title：Biophysical journal

authors： Traïkia M,Warschawski DE,Lambert O,Rigaud JL,Devaux PF

更新日期：2002-09-01 00:00:00

abstract：:Stepwise two-photon excitation of chlorophyll a and b in the higher plant main light-harvesting complex (LHC II) and the minor complex CP29 (as well as in organic solution) with 100-fs pulses in the Q(y) region results in a weak blue fluorescence. The dependence of the spectral shape of the blue fluorescence on excita...

journal_title：Biophysical journal

authors： Leupold D,Teuchner K,Ehlert J,Irrgang KD,Renger G,Lokstein H

更新日期：2002-03-01 00:00:00

abstract：:It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the a...

journal_title：Biophysical journal

authors： Girdhar G,Shao JY

更新日期：2007-12-01 00:00:00

abstract：:Studies have been performed on erythrocytes that have been subjected to a low shear stress of less than 100 dyn/cm(2) in a cone-and-plate viscometer. Alterations that were observed included decreased red cell survival, increased osmotic fragility, changes in the cation permeability of the red cell membrane, and a redu...

journal_title：Biophysical journal

authors： Nanjappa BN,Chang HK,Glomski CA

更新日期：1973-11-01 00:00:00

abstract：:The energy transfer and charge separation kinetics of a photosystem I (PS I) core particle of an antenna size of 100 chlorophyll/P700 has been studied by combined fluorescence and transient absorption kinetics with picosecond resolution. This is the first combined picosecond study of transient absorption and fluoresce...

journal_title：Biophysical journal

authors： Holzwarth AR,Schatz G,Brock H,Bittersmann E

更新日期：1993-06-01 00:00:00

abstract：:The patch-clamp technique of Mueller (1975, Ann. N.Y. Acad. Sci., 274:247-264) and Neher and Sakmann (1976, Nature (Lond.), 260:799-802) was modified to be suitable for single-channel measurements in lipid bilayers at potentials up to 500 mV. This method was used to study gramicidin A single-channel current-voltage ch...

journal_title：Biophysical journal

authors： Andersen OS

更新日期：1983-02-01 00:00:00

abstract：:Numerous isotypes of the structural protein tubulin have now been characterized in various organisms and their expression offers a plausible explanation for observed differences affecting microtubule function in vivo. While this is an attractive hypothesis, there are only a handful of studies demonstrating a direct in...

journal_title：Biophysical journal

authors： Rezania V,Azarenko O,Jordan MA,Bolterauer H,Ludueña RF,Huzil JT,Tuszynski JA

更新日期：2008-08-01 00:00:00

abstract：:Total internal reflection excitation used in combination with fluorescence correlation spectroscopy (TIR-FCS) is a method for characterizing the dynamic behavior and absolute concentrations of fluorescent molecules near or at the interface of a planar substrate and a solution. In this work, we demonstrate for the firs...

journal_title：Biophysical journal

authors： Lieto AM,Cush RC,Thompson NL

更新日期：2003-11-01 00:00:00

abstract：:By using an analogy between the magnetization of a paramagnetic material in an external magnetic field and the electric polarization of the lateral wall of outer hair cells in response to the transmembrane potential, we show that, based on experimental data on the charge transfer across the membrane, it is impossible ...

journal_title：Biophysical journal

authors： Scherer MP,Gummer AW

更新日期：2005-05-01 00:00:00