pH-dependent dimerization of the carboxyl terminal domain of Cx43.

Abstract:

:Previous studies have demonstrated that the carboxyl terminus of the gap junction protein Cx43 (Cx43CT) can act as an independent, regulatory domain that modulates intercellular communication in response to appropriate chemical stimuli. Here, we have used NMR, chemical cross-linking, and analytical ultracentrifugation to further characterize the biochemical and biophysical properties of the Connexin43 carboxyl terminal domain (S255-I382). NMR-diffusion experiments at pH 5.8 suggested that the Connexin43 carboxyl terminus (CX43CT) may have a molecular weight greater than that of a monomer. Sedimentation equilibrium and cross-linking data demonstrated a predominantly dimeric state for the Cx43CT at pH 5.8 and 6.5, with limited dimer formation at a more neutral pH. NMR-filtered nuclear Overhauser effect studies confirmed these observations and identified specific areas of parallel orientation within Cx43CT, likely corresponding to dimerization domains. These regions included a portion of the SH3 binding domain, as well as two fragments previously found to organize in alpha-helical structures. Together, these data show that acidification causes Cx43CT dimer formation in vitro. Whether dimer formation is an important structural component of the regulation of Connexin43 channels remains to be determined. Dimerization may alter the affinity of Cx43CT regions for specific molecular partners, thus modifying the regulation of gap junction channels.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Sorgen PL,Duffy HS,Spray DC,Delmar M

doi

10.1529/biophysj.103.039230

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

574-81

issue

1

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(04)73542-0

journal_volume

87

pub_type

杂志文章
  • Two progressive substrates of the M-intermediate can be identified in glucose-embedded, wild-type bacteriorhodopsin.

    abstract::Glucose-embedded bacteriorhodopsin shows M-intermediates with different Amide I infrared bands when samples are illuminated at 240 or 260 K, in contrast with fully hydrated samples where a single M-intermediate is formed at all temperatures. In hydrated, but not in glucose-embedded specimens, the N intermediate is for...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(94)80585-5

    authors: Vonck J,Han BG,Burkard F,Perkins GA,Glaeser RM

    更新日期:1994-09-01 00:00:00

  • Interfacial Binding Sites for Cholesterol on TRP Ion Channels.

    abstract::Transient receptor potential (TRP) channels are members of a large family of ion channels located in membranes rich in cholesterol, some of whose functions are affected by the cholesterol content of the membrane. Here, cholesterol binding to TRPs is studied using a docking procedure that allows the transmembrane surfa...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2019.10.011

    authors: Lee AG

    更新日期:2019-11-19 00:00:00

  • Bathoproducts of rhodopsin, isorhodopsin I, and isorhodopsin II.

    abstract::Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments a...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(80)85129-0

    authors: Mao B,Ebrey TG,Crouch R

    更新日期:1980-02-01 00:00:00

  • Reversing nerve cell pathology by optimizing modulatory action on target ion channels.

    abstract::In diseases of the brain, the distribution and properties of ion channels display deviations from healthy control subjects. We studied three cases of ion channel alteration related to epileptogenesis. The first case of ion channel alteration represents an enhanced sodium current, the second case addresses the downregu...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2011.08.055

    authors: Tigerholm J,Fransén E

    更新日期:2011-10-19 00:00:00

  • Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength.

    abstract::Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction limit emit separately when simultaneously exposed to excitation light. A basic mechanism for such transient fluo...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2010.08.012

    authors: Testa I,Wurm CA,Medda R,Rothermel E,von Middendorf C,Fölling J,Jakobs S,Schönle A,Hell SW,Eggeling C

    更新日期:2010-10-20 00:00:00

  • Reaction fields in the environment of fluorescent probes: polarity profiles in membranes.

    abstract::Fluorescent probes in biological systems are sensitive to environmental polarity by virtue of their response to the reaction field created by polarization of the dielectric medium. Classically, fluorophore solvatochromism is analyzed in terms of the Lippert equation and later variants, all of which rely upon the origi...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2009.01.006

    authors: Marsh D

    更新日期:2009-04-08 00:00:00

  • Slow inactivation in voltage gated potassium channels is insensitive to the binding of pore occluding peptide toxins.

    abstract::Voltage gated potassium channels open and inactivate in response to changes of the voltage across the membrane. After removal of the fast N-type inactivation, voltage gated Shaker K-channels (Shaker-IR) are still able to inactivate through a poorly understood closure of the ion conduction pore. This, usually slower, i...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.105.060152

    authors: Oliva C,González V,Naranjo D

    更新日期:2005-08-01 00:00:00

  • Orientational disorder and motion of weakly attached cross-bridges.

    abstract::In a relaxed muscle fiber at low ionic strength, the cross-bridges may well be in states comparable to the one that precedes the cross-bridge power stroke (Schoenberg, M. 1988. Adv. Exp. Med. Biol. 226:189-202). Using electron paramagnetic resonance (EPR) and (saturation transfer) electron paramagnetic resonance (ST-E...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(91)82093-8

    authors: Fajer PG,Fajer EA,Schoenberg M,Thomas DD

    更新日期:1991-09-01 00:00:00

  • Hydration of phosphatidylocholine. Adsorption isotherm and proton nuclear magnetic resonance studies.

    abstract::Adsorption-desorption isotherms were obtained for water binding by 1,2-dimyristoylphosphatidylcholine in the temperature range 15 degrees-35 degrees C. The isotherms were analyzed by Brunauer et al.'s (BET) theory and also a polarization theory, the latter being more successful in fitting the data. There was some evid...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(77)85542-2

    authors: Wilkinson DA,Morowitz HJ,Prestegard JH

    更新日期:1977-11-01 00:00:00

  • The role of semidisorder in temperature adaptation of bacterial FlgM proteins.

    abstract::Probabilities of disorder for FlgM proteins of 39 species whose optimal growth temperature ranges from 273 K (0°C) to 368 K (95°C) were predicted by a newly developed method called Sequence-based Prediction with Integrated NEural networks for Disorder (SPINE-D). We showed that the temperature-dependent behavior of Flg...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2013.10.026

    authors: Wang J,Yang Y,Cao Z,Li Z,Zhao H,Zhou Y

    更新日期:2013-12-03 00:00:00

  • Fast in silico protein folding by introduction of alternating hydrogen bond potentials.

    abstract::We accelerate protein folding in all-atom molecular dynamics simulations by introducing alternating hydrogen bond potentials as a supplement to the force field. The alternating hydrogen bond potentials result in accelerated hydrogen bond reordering, which leads to rapid formation of secondary structure elements. The m...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.107.122192

    authors: Wolf MG,de Leeuw SW

    更新日期:2008-05-15 00:00:00

  • Vibrational equilibration in absorption difference spectra of chlorophyll a.

    abstract::We describe Franck-Condon simulations of vibrational cooling effects on absorption difference spectra in chlorophyll a (Chl a). The relative contributions of vibrational equilibration in the electronic ground and excited states depend on the pump and probe wavelengths. For Franck-Condon-active vibrational modes exhibi...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(95)80145-1

    authors: Struve WS

    更新日期:1995-12-01 00:00:00

  • Side-chain structure and dynamics at the lipid-protein interface: Val1 of the gramicidin A channel.

    abstract::High resolution dynamics and structural information has been resolved from 2H solid-state NMR spectra of the Val-1 side-chain of the gramicidin channel in a lipid bilayer. Both powder pattern lineshapes and spectra from uniformly aligned samples of gramicidin in lipid bilayers have been analyzed to achieve a fully con...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(94)80928-2

    authors: Lee KC,Cross TA

    更新日期:1994-05-01 00:00:00

  • The stiffness of the flagella of impaled bull sperm.

    abstract::The elastic rigidity (stiffness) of impaled motionless bull sperm flagella has been determined by a manipulatory technique which permitted direct analytical treatment of the experimental system. The effects of external ATP and ADP were measured. It was found that ATP acts as a plasticizing agent, while ADP does not. T...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(73)85997-1

    authors: Lindemann CB,Rudd WG,Rikmenspoel R

    更新日期:1973-05-01 00:00:00

  • Comparison of the biophysical properties of racemic and d-erythro-N-acyl sphingomyelins.

    abstract::In this study stereochemically pure d-erythro-sphingomyelins (SMs) with either 16:0 or 18:1(cisDelta9) as the N-linked acyl-chain were synthesized. Our purpose was to examine the properties of these sphingomyelins and acyl-chain matched racemic (d-erythro/l-threo) sphingomyelins in model membranes. Liquid-expanded d-e...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(99)76997-3

    authors: Ramstedt B,Slotte JP

    更新日期:1999-09-01 00:00:00

  • Do All X-ray Structures of Protein-Ligand Complexes Represent Functional States? EPOR, a Case Study.

    abstract::Based on differences between the x-ray crystal structures of ligand-bound and unbound forms, the activation of the erythropoietin receptor (EPOR) was initially proposed to involve a cross-action scissorlike motion. However, the validity of the motions involved in the scissorlike model has been recently challenged. Her...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2016.12.042

    authors: Corbett MSP,Mark AE,Poger D

    更新日期:2017-02-28 00:00:00

  • Asymmetrical membranes and surface tension.

    abstract::The (31)P-nuclear magnetic resonance chemical shift of phosphatidic acid in a membrane is sensitive to the lipid head group packing and can report qualitatively on membrane lateral compression near the aqueous interface. We have used high-resolution (31)P-nuclear magnetic resonance to evaluate the lateral compression ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(02)73915-5

    authors: Traïkia M,Warschawski DE,Lambert O,Rigaud JL,Devaux PF

    更新日期:2002-09-01 00:00:00

  • Measuring the Refractive Index of Bovine Corneal Stromal Cells Using Quantitative Phase Imaging.

    abstract::The cornea is the primary refractive lens in the eye and transmits >90% of incident visible light. It has been suggested that the development of postoperative corneal haze could be due to an increase in light scattering from activated corneal stromal cells. Quiescent keratocytes are thought to produce crystallins that...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2015.08.046

    authors: Gardner SJ,White N,Albon J,Knupp C,Kamma-Lorger CS,Meek KM

    更新日期:2015-10-20 00:00:00

  • Temperature derivative fluorescence spectroscopy as a tool to study dynamical changes in protein crystals.

    abstract::Motions through the energy landscape of proteins lead to biological function. At temperatures below a dynamical transition (150-250 K), some of these motions are arrested and the activity of some proteins ceases. Here, we introduce the technique of temperature-derivative fluorescence microspectrophotometry to investig...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(04)74365-9

    authors: Weik M,Vernede X,Royant A,Bourgeois D

    更新日期:2004-05-01 00:00:00

  • Conformational heterogeneity of creatine kinase determined from phase resolved fluorometry.

    abstract::Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(91)82274-3

    authors: Grossman SH

    更新日期:1991-03-01 00:00:00

  • Molecular dynamics of alamethicin transmembrane channels from open-channel current noise analysis.

    abstract::Conductance noise measurement of the open states of alamethicin transmembrane channels reveals excess noise attributable to cooperative low-frequency molecular dynamics that can generate fluctuations approximately 1 A rms in the effective channel pore radius. Single-channel currents through both persistent and nonpers...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(95)80103-7

    authors: Mak DO,Webb WW

    更新日期:1995-12-01 00:00:00

  • Model-driven designs of an oscillating gene network.

    abstract::The current rapid expansion of biological knowledge offers a great opportunity to rationally engineer biological systems that respond to signals such as light and chemical inducers by producing specific proteins. Turning on and off the production of proteins on demand holds great promise for creating significant biote...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.105.064204

    authors: Tuttle LM,Salis H,Tomshine J,Kaznessis YN

    更新日期:2005-12-01 00:00:00

  • Modeling of Noisy Spindle Dynamics Reveals Separable Contributions to Achieving Correct Orientation.

    abstract::The mechanisms by which the mammalian mitotic spindle is guided to a predefined orientation through microtubule-cortex interactions have recently received considerable interest, but there has been no dynamic model that describes spindle movements toward the preferred axis in human cells. Here, we develop a dynamic mod...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2015.08.014

    authors: Corrigan AM,Shrestha R,Draviam VM,Donald AM

    更新日期:2015-10-06 00:00:00

  • Particulate nature of blood determines macroscopic rheology: a 2-D lattice Boltzmann analysis.

    abstract::Historically, predicting macroscopic blood flow characteristics such as viscosity has been an empirical process due to the difficulty in rigorously including the particulate nature of blood in a mathematical representation of blood rheology. Using a two-dimensional lattice Boltzmann approach, we have simulated the flo...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.104.051151

    authors: Sun C,Munn LL

    更新日期:2005-03-01 00:00:00

  • Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.

    abstract::The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3'-deac-eda-ADP (deac-ed...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(04)74289-7

    authors: Khromov AS,Webb MR,Ferenczi MA,Trentham DR,Somlyo AP,Somlyo AV

    更新日期:2004-04-01 00:00:00

  • Insight into the putative specific interactions between cholesterol, sphingomyelin, and palmitoyl-oleoyl phosphatidylcholine.

    abstract::The effects of cholesterol (Chol) on phospholipid bilayers include ordering of the fatty acyl chains, condensing of the lipids in the bilayer plane, and promotion of the liquid-ordered phase. These effects depend on the type of phospholipids in the bilayer and are determined by the nature of the underlying molecular i...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.106.088427

    authors: Aittoniemi J,Niemelä PS,Hyvönen MT,Karttunen M,Vattulainen I

    更新日期:2007-02-15 00:00:00

  • Comparative Fourier transform infrared spectroscopy study of cold-, pressure-, and heat-induced unfolding and aggregation of myoglobin.

    abstract::We studied the cold unfolding of myoglobin with Fourier transform infrared spectroscopy and compared it with pressure and heat unfolding. Because protein aggregation is a phenomenon with medical as well as biotechnological implications, we were interested in both the structural changes as well as the aggregation behav...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(02)75605-1

    authors: Meersman F,Smeller L,Heremans K

    更新日期:2002-05-01 00:00:00

  • Extracting the causality of correlated motions from molecular dynamics simulations.

    abstract::The information theory measure of transfer entropy is used to extract the causality of correlated motions from molecular dynamics simulations. For each pair of correlated residues, the method quantifies which residue drives the correlated motions, and which residue responds. The measure reveals how correlated motions ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2009.07.019

    authors: Kamberaj H,van der Vaart A

    更新日期:2009-09-16 00:00:00

  • Protein-induced surface structuring in myelin membrane monolayers.

    abstract::Monolayers prepared from myelin conserve all the compositional complexity of the natural membrane when spread at the air-water interface. They show a complex pressure-dependent surface pattern that, on compression, changes from the coexistence of two liquid phases to a viscous fractal phase embedded in a liquid phase....

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.107.112441

    authors: Rosetti CM,Maggio B

    更新日期:2007-12-15 00:00:00

  • Tuning the voltage-sensor motion with a single residue.

    abstract::The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzymes regulated by a voltage-sensor domain (VSD) related to the VSD found in voltage-gated ion channels. Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics compared to ion chann...

    journal_title:Biophysical journal

    pub_type: 信件

    doi:10.1016/j.bpj.2012.06.030

    authors: Lacroix JJ,Bezanilla F

    更新日期:2012-08-08 00:00:00