Synchronization of circadian oscillation of phosphorylation level of KaiC in vitro.

abstract：:ATP-sensitive K(+)-channels link metabolism and excitability in neurons, myocytes, and pancreatic islets. Mutations in the pore-forming subunit (Kir6.2; KCNJ11) cause neonatal diabetes, developmental delay, and epilepsy by decreasing sensitivity to ATP inhibition and suppressing electrical activity. Mutations of resid...

journal_title：Biophysical journal

authors： Koster JC,Kurata HT,Enkvetchakul D,Nichols CG

更新日期：2008-11-15 00:00:00

abstract：:Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models are constructed computationally by building specific regions of the amino acid sequence as alpha helices and then positio...

journal_title：Biophysical journal

authors： Trewhella J,Anderson S,Fox R,Gogol E,Khan S,Engelman D,Zaccai G

更新日期：1983-06-01 00:00:00

abstract：:Cholesterol is a key component of eukaryotic membranes, but its role in cellular biology in general and in lipid rafts in particular remains controversial. Model membranes are used extensively to determine the phase behavior of ternary mixtures of cholesterol, a saturated lipid, and an unsaturated lipid with liquid-or...

journal_title：Biophysical journal

authors： Bennett WFD,Shea JE,Tieleman DP

更新日期：2018-06-05 00:00:00

abstract：:Many cell cytoskeletons include an aster of microtubules, with the centrosome serving as the focal point. The position of the centrosome within the cell is important in such directional activities as wound closure and interactions of immune cells. Here we analyzed the centrosome positioning as it is dictated by microt...

journal_title：Biophysical journal

authors： Maly VI,Maly IV

更新日期：2010-11-03 00:00:00

abstract：:A dynamic model of smooth muscle contraction is presented and is compared with the mechanical properties of vascular smooth muscle in the rat portal vein. The model is based on the sliding filament theory and the assumption that force is produced by cross-bridges extending from the myosin to the actin filaments. Thus,...

journal_title：Biophysical journal

authors： Gestrelius S,Borgström P

更新日期：1986-07-01 00:00:00

abstract：:Based on the existence of ATP-sensitive potassium channels in the plasma membrane of pancreatic beta cells, we develop a quantitative explanation of the electrical activity observed in pancreatic islets. The proposed mechanism involves the voltage-dependent inward calcium and outward potassium currents described by Ro...

journal_title：Biophysical journal

authors： Keizer J,Magnus G

更新日期：1989-08-01 00:00:00

abstract：:Critical to biological processes such as secretion and transport, protein-lipid interactions within the membrane and at the membrane-water interface still raise many questions. Here we examine the role of lipid headgroups in these interactions by using gramicidin A (gA) channels in planar bilayers as a probe. We show ...

journal_title：Biophysical journal

authors： Rostovtseva TK,Petrache HI,Kazemi N,Hassanzadeh E,Bezrukov SM

更新日期：2008-02-15 00:00:00

abstract：:Solid phase amplification (SPA), a new method to amplify DNA, is characterized by the use of surface-bound primers. This limits the amplification to two-dimensional surfaces and therefore allows the easy parallelization of DNA amplification in a single system. SPA leads to the formation of small but dense DNA brushes,...

journal_title：Biophysical journal

authors： Mercier JF,Slater GW

更新日期：2005-07-01 00:00:00

abstract：:Solid-state NMR study shows that the 22-residue K3 peptide (Ser(20)-Lys(41)) from beta(2)-microglobulin (beta(2)m) adopts a beta-strand-loop-beta-strand conformation in its fibril state. Residue Pro(32) has a trans conformation in the fibril state of the peptide, while it adopts a cis conformation in the native state ...

journal_title：Biophysical journal

authors： Liang C,Derreumaux P,Mousseau N,Wei G

更新日期：2008-07-01 00:00:00

abstract：:The relevance of various residue positions for the stability and the folding characteristics of the prion protein in its normal cellular form are investigated by using molecular dynamics simulations of models exploiting the topology of the native state. These models allow for reproducing the experimentally validated t...

journal_title：Biophysical journal

authors： Settanni G,Hoang TX,Micheletti C,Maritan A

更新日期：2002-12-01 00:00:00

abstract：:Stabilized intermediate redox states of cytochrome c are generated by radiolytic reduction of initially oxidized enzyme in glass matrices at liquid nitrogen temperature. In the intermediate states the heme group is reduced by hydrated electrons, whereas the protein conformation is restrained close to its oxidized form...

journal_title：Biophysical journal

authors： Cartling B

更新日期：1983-08-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) has been used to measure the diffusion of fluorescently labeled beads in solutions of polymerized actin or buffer. The results, obtained at actin concentrations of 1 mg/ml, show that small beads (0.09 micron in diameter) diffuse nearly as rapidly in the actin gel as in buffe...

journal_title：Biophysical journal

authors： Qian H,Elson EL,Frieden C

更新日期：1992-10-01 00:00:00

abstract：:Formation of low-temperature ordered gel phases in several fully hydrated phosphatidylethanolamines (PEs) and phosphatidylcholines (PCs) with saturated chains as well as in dipalmitoylphosphatidylglycerol (DPPG) was observed by synchrotron x-ray diffraction, microcalorimetry, and densitometry. The diffraction patterns...

journal_title：Biophysical journal

authors： Tenchov B,Koynova R,Rapp G

更新日期：2001-04-01 00:00:00

abstract：:The active amplification of sound-induced vibrations in the cochlea, known to be crucial for auditory sensitivity and frequency selectivity, is not well understood. The outer hair cell (OHC) somatic electromotility is a potential mechanism for such amplification. Its effectiveness in vivo is putatively limited by the ...

journal_title：Biophysical journal

authors： Ramamoorthy S,Nuttall AL

更新日期：2012-02-08 00:00:00

abstract：:Although actin and myosin are important contributors to cell-force generation, shape change, and motility, their contributions to cell stiffness and frequency-dependent rheology have not been conclusively determined. We apply several pharmacological interventions to cultured epithelial cells to elucidate the roles of ...

journal_title：Biophysical journal

authors： Van Citters KM,Hoffman BD,Massiera G,Crocker JC

更新日期：2006-11-15 00:00:00

abstract：:Cationic lipid-DNA (CL-DNA) complexes are abundantly used in nonviral gene therapy clinical applications. Surface functionality is the next step in developing these complexes as competent, target-specific gene carriers. Poly(ethylene glycol) (PEG) is the natural choice to serve as a protective coat or act as a tether ...

journal_title：Biophysical journal

authors： Martin-Herranz A,Ahmad A,Evans HM,Ewert K,Schulze U,Safinya CR

更新日期：2004-02-01 00:00:00

abstract：:Chimeric antigen receptors (CARs) have recently been approved for the treatment of hematological malignancies, but our lack of understanding of the basic mechanisms that activate these proteins has made it difficult to optimize and control CAR-based therapies. In this study, we use phosphoproteomic mass spectrometry a...

journal_title：Biophysical journal

authors： Rohrs JA,Zheng D,Graham NA,Wang P,Finley SD

更新日期：2018-09-18 00:00:00

abstract：:Some low molecular weight biomolecules, i.e., NAD(P)H, are unstable at high temperatures. The use of these biomolecules by thermophilic microorganisms has been scarcely analyzed. Herein, NADH stability has been studied at different temperatures and viscosities. NADH decay increased at increasing temperatures. At incre...

journal_title：Biophysical journal

authors： Cuecas A,Cruces J,Galisteo-López JF,Peng X,Gonzalez JM

更新日期：2016-08-23 00:00:00

abstract：:Red fluorescent proteins are important tools in fluorescence-based life science research. Recently, we have introduced eqFP611, a red fluorescent protein with advantageous properties from the sea anemone Entacmaea quadricolor. Here, we have studied the submillisecond light-driven intramolecular dynamics between bright...

journal_title：Biophysical journal

authors： Schenk A,Ivanchenko S,Röcker C,Wiedenmann J,Nienhaus GU

更新日期：2004-01-01 00:00:00

abstract：:Cell migration is based on an actin treadmill, which in turn depends on recycling of G-actin across the cell, from the rear where F-actin disassembles, to the front, where F-actin polymerizes. To analyze the rates of the actin transport, we used the Virtual Cell software to solve the diffusion-drift-reaction equations...

journal_title：Biophysical journal

authors： Novak IL,Slepchenko BM,Mogilner A

更新日期：2008-08-01 00:00:00

abstract：:Adenylate kinase, an enzyme that catalyzes the phosphoryl transfer between ATP and AMP, can interconvert between the open and catalytically potent (closed) forms even without binding ligands. Several aspects of the enzyme elasticity and internal dynamics are analyzed here by atomistic molecular dynamics simulations co...

journal_title：Biophysical journal

authors： Pontiggia F,Zen A,Micheletti C

更新日期：2008-12-15 00:00:00

abstract：:The relationship between calcium current and transmitter release was studied in squid giant synapse. It was found that the voltage-dependent calcium current triggers the release of synaptic transmitter in direct proportion to its magnitude and duration. Transmitter release occurs with a delay of approximately 200 mus ...

journal_title：Biophysical journal

authors： Llinás R,Steinberg IZ,Walton K

更新日期：1981-03-01 00:00:00

abstract：:Recently various pathways of human telomere (ht) DNA folding into G-quadruplexes and of ligand binding to these structures have been proposed. However, the key issue as to the nature of forces driving the folding and recognition processes remains unanswered. In this study, structural changes of 22-mer ht-DNA fragment ...

journal_title：Biophysical journal

authors： Bončina M,Hamon F,Islam B,Teulade-Fichou MP,Vesnaver G,Haider S,Lah J

更新日期：2015-06-16 00:00:00

abstract：:The circadian clocks keeping time in many living organisms rely on self-sustained biochemical oscillations entrained by external cues, such as light, to the 24-h cycle induced by Earth's rotation. However, environmental cues are unreliable due to the variability of habitats, weather conditions, or cue-sensing mechanis...

journal_title：Biophysical journal

authors： Pfeuty B,Thommen Q,Lefranc M

更新日期：2011-06-08 00:00:00

abstract：:It was previously reported (Chernomordik and Sowers, 1991) that erythrocyte ghosts which were exposed to a 42 degrees C, 10-min heat treatment would, upon electrofusion, produce over 15-20 s a fusion product with an "open lumen" (i.e., the fusion product became converted to one large sphere), while electrofusion of gh...

journal_title：Biophysical journal

authors： Wu Y,Sjodin RA,Sowers AE

更新日期：1994-01-01 00:00:00

abstract：:Sinusoidal oscillatory flow of blood and of aqueous glycerol solutions was produced in rigid cylindrical tubes. For aqueous glycerol, the amplitude of the measured pressure gradient wave form conformed closely to that predicted by Womersley's theory of oscillatory flow, up to Reynolds numbers approaching 2000. Blood d...

journal_title：Biophysical journal

authors： Kunz AL,Coulter NA Jr

更新日期：1967-01-01 00:00:00

abstract：:The vessel wall experiences progressive stiffening with age and the development of cardiovascular disease, which alters the micromechanical environment experienced by resident vascular smooth muscle cells (VSMCs). In vitro studies have shown that VSMCs are sensitive to substrate stiffness, but the exact molecular mech...

journal_title：Biophysical journal

authors： Sazonova OV,Lee KL,Isenberg BC,Rich CB,Nugent MA,Wong JY

更新日期：2011-08-03 00:00:00

abstract：:Intramolecular electron transfer between CuA and heme a in solubilized bacterial (Paracoccus denitrificans) cytochrome c oxidase was investigated by pulse radiolysis. CuA, the initial electron acceptor, was reduced by 1-methylnicotinamide radicals in a diffusion-controlled reaction, as monitored by absorption changes ...

journal_title：Biophysical journal

authors： Farver O,Grell E,Ludwig B,Michel H,Pecht I

更新日期：2006-03-15 00:00:00

abstract：:The capability to image single microbial cell surfaces at nanometer scale under native conditions would profoundly impact mechanistic and structural studies of pathogenesis, immunobiology, environmental resistance, and biotransformation. Here, using in vitro atomic force microscopy, we have directly visualized high-re...

journal_title：Biophysical journal

authors： Plomp M,Leighton TJ,Wheeler KE,Malkin AJ

更新日期：2005-01-01 00:00:00

abstract：:Outside-out patches of enzymatically dissociated adult and denervated mouse muscle fibers were superfused repetitively by pulses of acetylcholine (ACh) containing solution. Up to 300 channels opened simultaneously 300 microseconds after the beginning of a 1,000 microM ACh pulse corresponding to a peak current i of alm...

journal_title：Biophysical journal

authors： Franke C,Hatt H,Parnas H,Dudel J

更新日期：1991-11-01 00:00:00