Studies on the structure of actin gels using time correlation spectroscopy of fluorescent beads.

abstract：:We investigate the interactions between lipid bilayers and amphiphilic peptides using a solvent-free coarse-grained simulation technique. In our model, each lipid is represented by one hydrophilic and three hydrophobic beads. The amphiphilic peptide is modeled as a hydrophobic-hydrophilic cylinder with hydrophilic cap...

journal_title：Biophysical journal

authors： Illya G,Deserno M

更新日期：2008-11-01 00:00:00

abstract：:The recently developed CMAP correction to the CHARMM22 force field (C22) is evaluated from 25 ns molecular dynamics simulations on hen lysozyme. Substantial deviations from experimental backbone root mean-square fluctuations and N-H NMR order parameters obtained in the C22 trajectories (especially in the loops) are el...

journal_title：Biophysical journal

authors： Buck M,Bouguet-Bonnet S,Pastor RW,MacKerell AD Jr

更新日期：2006-02-15 00:00:00

abstract：:Molecular dynamics simulations of protein folding and unfolding are often carried out at temperatures (400-600 K) that are much higher than physiological or room temperature to speed up the (un)folding process. Use of such high temperatures changes both the protein and solvent properties considerably, compared to phys...

journal_title：Biophysical journal

authors： Walser R,Mark AE,van Gunsteren WF

更新日期：2000-06-01 00:00:00

abstract：:Lateral tension in cell plasma membranes plays an essential role in regulation of a number of membrane-related intracellular processes and cell motion. Understanding the physical factors generating the lateral tension and quantitative determination of the tension distribution along the cell membrane is an emerging top...

journal_title：Biophysical journal

authors： Schweitzer Y,Lieber AD,Keren K,Kozlov MM

更新日期：2014-01-07 00:00:00

abstract：:The nucleus has a smooth, regular appearance in normal cells, and its shape is greatly altered in human pathologies. Yet, how the cell establishes nuclear shape is not well understood. We imaged the dynamics of nuclear shaping in NIH3T3 fibroblasts. Nuclei translated toward the substratum and began flattening during t...

journal_title：Biophysical journal

authors： Li Y,Lovett D,Zhang Q,Neelam S,Kuchibhotla RA,Zhu R,Gundersen GG,Lele TP,Dickinson RB

更新日期：2015-08-18 00:00:00

abstract：:Circadian rhythms possess the ability to robustly entrain to the environmental cycles. This ability relies on the phase synchronization of circadian rhythm gene regulation to different environmental cues, of which light is the most obvious and important. The elucidation of the mechanism of circadian entrainment requir...

journal_title：Biophysical journal

authors： Gunawan R,Doyle FJ 3rd

更新日期：2006-09-15 00:00:00

abstract：:To investigate diffusion processes in agarose gel, nanoparticles with sizes in the range between 1 and 140 nm have been tested by means of fluorescence correlation spectroscopy. Understanding the diffusion properties in agarose gels is interesting, because such gels are good models for microbial biofilms and cells cyt...

journal_title：Biophysical journal

authors： Fatin-Rouge N,Starchev K,Buffle J

更新日期：2004-05-01 00:00:00

abstract：:Cytoplasmic free Mg(2+) concentration ([Mg(2+)]i) was measured in rat ventricular myocytes with a fluorescent indicator furaptra (mag-fura-2) introduced by AM-loading. By incubation of the cells in a high-K(+) (Ca(2+)- and Mg(2+)-free) solution, [Mg(2+)]i decreased from ? 0.9 mM to 0.2 to 0.5 mM. The lowered [Mg(2+)]i...

journal_title：Biophysical journal

authors： Tashiro M,Inoue H,Konishi M

更新日期：2014-11-04 00:00:00

abstract：:High resolution dynamics and structural information has been resolved from 2H solid-state NMR spectra of the Val-1 side-chain of the gramicidin channel in a lipid bilayer. Both powder pattern lineshapes and spectra from uniformly aligned samples of gramicidin in lipid bilayers have been analyzed to achieve a fully con...

journal_title：Biophysical journal

authors： Lee KC,Cross TA

更新日期：1994-05-01 00:00:00

abstract：:The process of clathrin self-assembly into closed shells with the Fullerene structure is investigated. It is argued that the shell size distribution is governed by the kinetics of assembly and depends on the rate of growth controlled by the free clathrin concentration. The particularly abundant small structures--the "...

journal_title：Biophysical journal

authors： Shraiman BI

更新日期：1997-02-01 00:00:00

abstract：:The cytoplasm of a living cell is a dynamic environment through which intracellular components must move and mix. In motile, rapidly deforming cells such as human neutrophils, bulk cytoplasmic flow couples cell deformation to the transport and dispersion of cytoplasmic particles. Using particle-tracking measurements i...

journal_title：Biophysical journal

authors： Koslover EF,Chan CK,Theriot JA

更新日期：2017-11-07 00:00:00

abstract：:We have studied the combined effects of X-irradiation and nitrogen mustard treatment on the colony-forming ability of Chinese hamster cells. In contrast to X-irradiation, nitrogen mustard acting by itself yields an exponential dose-effect relationship. Moreover, whether delivered in immediate sequence or after varying...

journal_title：Biophysical journal

authors： Sakamoto K,Elkind MM

更新日期：1969-09-01 00:00:00

abstract：:Alkaline sucrose sedimentation studies of DNA from mouse lymphoma cells (L5178Y) treated with caffeine have demonstrated the following effects. Caffeine (at a concentration of 1.6 mM) does not introduce strand breaks into preformed DNA nor does it inhibit the rejoining of gamma-ray-induced strand breaks. Although it d...

journal_title：Biophysical journal

authors： Lehmann AR

更新日期：1972-10-01 00:00:00

abstract：:Open channel properties of canine cardiac Purkinje cell Na+ channels were studied with single channel cell-attached recording and with whole cell macroscopic current recording in internally perfused cells. Single channel currents and membrane currents increased with an increase in Na+ concentration, but showed evidenc...

journal_title：Biophysical journal

authors： Sheets MF,Scanley BE,Hanck DA,Makielski JC,Fozzard HA

更新日期：1987-07-01 00:00:00

abstract：:One theory of freezing damage suggests that slowly cooled cells are killed by being exposed to increasing concentrations of electrolytes as the suspending medium freezes. A corollary to this view is that protective additives such as glycerol protect cells by acting colligatively to reduce the electrolyte concentration...

journal_title：Biophysical journal

authors： Rall WF,Mazur P,Souzu H

更新日期：1978-07-01 00:00:00

abstract：:In the photocycle of bacteriorhodopsin at pH 7, a proton is ejected to the extracellular medium during the protonation of Asp-85 upon formation of the M intermediate. The group that releases the ejected proton does not become reprotonated until the prephotolysis state is restored from the N and O intermediates. In con...

journal_title：Biophysical journal

authors： Morgan JE,Vakkasoglu AS,Lanyi JK,Lugtenburg J,Gennis RB,Maeda A

更新日期：2012-08-08 00:00:00

abstract：:It has been a significant challenge to quantitatively study the dynamic intracellular processes in live cells. These studies are essential for a thorough understanding of the underlying mechanisms regulating the signaling pathways and the transitions between cell cycle stages. Our studies of Cdc20, an important mitoti...

journal_title：Biophysical journal

authors： Wang Z,Shah JV,Berns MW,Cleveland DW

更新日期：2006-07-01 00:00:00

abstract：:Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resol...

journal_title：Biophysical journal

authors： Gustafsson MG,Shao L,Carlton PM,Wang CJ,Golubovskaya IN,Cande WZ,Agard DA,Sedat JW

更新日期：2008-06-01 00:00:00

abstract：:Proliferation of mammalian cells, even under conditions of unlimited growth, presents a complex problem because of the interaction of deterministic and stochastic processes. Division of the cell cycle into a finite number of parts establishes a multidimensional vector space. In this space an arbitrary culture can be r...

journal_title：Biophysical journal

authors： Hahn GM

更新日期：1966-05-01 00:00:00

abstract：:A beat-to-beat variation in the cardiac action potential duration is a phenomenon known as alternans. Alternans has been linked to ventricular fibrillation, and thus the ability to predict the onset of alternans could be clinically beneficial. Theoretically, it has been proposed that the slope of a restitution curve, ...

journal_title：Biophysical journal

authors： Cram AR,Rao HM,Tolkacheva EG

更新日期：2011-02-16 00:00:00

abstract：:In permeabilized single fibers of fast (psoas) and slow (soleus) muscle from the rabbit, the effect of pH on isometric myofibrillar ATPase activity and force was studied at 15 degrees C, in the pH range 6.4-7.9. ATPase activity was measured photometrically by enzymatic coupling of the regeneration of ATP to the oxidat...

journal_title：Biophysical journal

authors： Potma EJ,van Graas IA,Stienen GJ

更新日期：1994-12-01 00:00:00

abstract：:Nuclear transport receptors (NTRs) mediate nucleocytoplasmic transport via their affinity for unstructured proteins (polymers) in the nuclear pore complex (NPC). Here, we have modeled the effect of NTRs on polymeric structure in the nanopore confinement of the NPC central conduit. The model explicitly takes into accou...

journal_title：Biophysical journal

authors： Osmanović D,Ford IJ,Hoogenboom BW

更新日期：2013-12-17 00:00:00

abstract：:Structural transition can be induced in charged micelles by increasing the ionic strength of the medium. We have monitored the organization and dynamics of the functionally important tryptophan residues of gramicidin in spherical and rod-shaped sodium dodecyl sulfate micelles utilizing a combination of wavelength-sele...

journal_title：Biophysical journal

authors： Rawat SS,Kelkar DA,Chattopadhyay A

更新日期：2005-11-01 00:00:00

abstract：:Maintenance of paroxysmal atrial fibrillation (AF) by fast rotors in the left atrium (LA) or at the pulmonary veins (PVs) is not fully understood. To gain insight into this dynamic and complex process, we studied the role of the heterogeneous distribution of transmembrane currents in the PVs and LA junction (PV-LAJ) i...

journal_title：Biophysical journal

authors： Calvo CJ,Deo M,Zlochiver S,Millet J,Berenfeld O

更新日期：2014-04-15 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and phospholamban (PLB) are essential for intracellular Ca2+ transport in myocytes. Ca2+-dependent activation of SERCA-PLB provides a control function that regulates cytosolic and SR Ca2+ levels. Although experimental and computational studies alone have led to a greater...

journal_title：Biophysical journal

authors： Raguimova ON,Aguayo-Ortiz R,Robia SL,Espinoza-Fonseca LM

更新日期：2020-11-03 00:00:00

abstract：:The spermine-induced DNA condensation is a first-order phase transition. Here, we apply a novel technique fluorescence lifetime correlation spectroscopy to analyze this transition in a greater detail. We show that the method allows for the observation of the condensed and uncondensed molecules simultaneously based sol...

journal_title：Biophysical journal

authors： Humpolícková J,Benda A,Sýkora J,Machán R,Kral T,Gasinska B,Enderlein J,Hof M

更新日期：2008-02-01 00:00:00

abstract：:We have measured the fluorescence spectra of a single fluorophore attached to a single protein molecule in aqueous solution using a total internal reflection fluorescence microscope. The most reactive cysteine residue of myosin subfragment-1 (S1) was labeled with tetramethylrhodamine. The spectral shift induced by a c...

journal_title：Biophysical journal

authors： Wazawa T,Ishii Y,Funatsu T,Yanagida T

更新日期：2000-03-01 00:00:00

abstract：:In most environments, such as natural aquatic systems, bacteria are found predominantly in self-organized sessile communities known as biofilms. In the presence of a significant flow, mature multispecies biofilms often develop into long filamentous structures called streamers, which can greatly influence ecosystem pro...

journal_title：Biophysical journal

authors： Rusconi R,Lecuyer S,Autrusson N,Guglielmini L,Stone HA

更新日期：2011-03-16 00:00:00

abstract：:The mechanism of pH-triggered destabilization of liposomes composed of a polyethyleneglycol-orthoester-distearoylglycerol lipid (POD) and phosphatidyl ethanolamine (PE) has been studied using an ANTS/DPX leakage and a lipid-mixing assay. We developed a kinetic model that relates POD hydrolysis to liposome collapse. Th...

journal_title：Biophysical journal

authors： Guo X,MacKay JA,Szoka FC Jr

更新日期：2003-03-01 00:00:00

abstract：:Apolipoprotein H (ApoH), also known as beta(2)-glycoprotein I, is a plasma glycoprotein with its in vivo physiological and pathogenic roles being closely related to its interaction with negatively charged membranes. Although the three-dimensional crystal structure of ApoH has been recently solved, direct evidence abou...

journal_title：Biophysical journal

authors： Wang F,Xia XF,Sui SF

更新日期：2002-08-01 00:00:00