Theoretical analysis of membrane tension in moving cells.

Abstract:

:Lateral tension in cell plasma membranes plays an essential role in regulation of a number of membrane-related intracellular processes and cell motion. Understanding the physical factors generating the lateral tension and quantitative determination of the tension distribution along the cell membrane is an emerging topic of cell biophysics. Although experimental data are accumulating on membrane tension values in several cell types, the tension distribution along the membranes of moving cells remains largely unexplored. Here we suggest and analyze a theoretical model predicting the tension distribution along the membrane of a cell crawling on a flat substrate. We consider the tension to be generated by the force of actin network polymerization against the membrane at the cell leading edge. The three major factors determining the tension distribution are the membrane interaction with anchors connecting the actin network to the lipid bilayer, the membrane interaction with cell adhesions, and the force developing at the rear boundary due to the detachment of the remaining cell adhesion from the substrate in the course of cell crawling. Our model recovers the experimentally measured values of the tension in fish keratocytes and their dependence on the number of adhesions. The model predicts, quantitatively, the tension distribution between the leading and rear membrane edges as a function of the area fractions of the anchors and the adhesions.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Schweitzer Y,Lieber AD,Keren K,Kozlov MM

doi

10.1016/j.bpj.2013.11.009

subject

Has Abstract

pub_date

2014-01-07 00:00:00

pages

84-92

issue

1

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(13)01237-X

journal_volume

106

pub_type

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