X-rays and nitrogen mustard: independent action in Chinese hamster cells.

abstract：:Flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD) are essential flavoprotein cofactors. A riboflavin kinase (RFK) activity catalyzes riboflavin phosphorylation to FMN, which can then be transformed into FAD by an FMN:adenylyltransferase (FMNAT) activity. Two enzymes are responsible for each one of thes...

journal_title：Biophysical journal

authors： Lans I,Seco J,Serrano A,Burbano R,Cossio P,Daza MC,Medina M

更新日期：2018-09-18 00:00:00

abstract：:In this paper, we report on the presence of cation binding areas on bovine serum amine oxidase, where metal ions of the groups IA and IIA, such as Na(+), K(+), Cs(+), Mg(2+), and Ca(2+), bind with various affinities. We found a cation-binding area that influences the enzyme activity if occupied, so that the catalytic ...

journal_title：Biophysical journal

authors： Di Paolo ML,Scarpa M,Corazza A,Stevanato R,Rigo A

更新日期：2002-10-01 00:00:00

abstract：:It has long been recognized that protein dynamical processes occur over a wide temporal range. However, the functionality of this spectrum of events remains unclear. In this work, a generalized noise function analysis is applied to a collection of diverse protein dynamical systems. It is shown that a power law model w...

journal_title：Biophysical journal

authors： Dewey TG,Bann JG

更新日期：1992-08-01 00:00:00

abstract：:Force spectroscopy is commonly used to measure the kinetics of processes occurring in single biological molecules. These measurements involve attaching the molecule of interest to micron-sized or larger force probes via compliant linkers. Recent theoretical work has described how the properties of the probes and linke...

journal_title：Biophysical journal

authors： Neupane K,Woodside MT

更新日期：2016-07-26 00:00:00

abstract：:Catch force in molluscan smooth muscle requires little, if any, energy input and is controlled by the phosphorylation state of the thick filament-associated mini-titin, twitchin. The kinetic parameters of myosin cross-bridge turnover in permeabilized catch muscle, and how they are potentially modified by the catch mec...

journal_title：Biophysical journal

authors： Franke AS,Mooers SU,Narayan SR,Siegman MJ,Butler TM

更新日期：2007-07-15 00:00:00

abstract：:The cell membrane having a transport system is inferred to be flexible when its function is being activated. For the brush border membrane vesicles prepared from rat small intestine, which have the co-transport system of Na+ and glucose, the membrane elasticity was measured as a function of the d-glucose concentration...

journal_title：Biophysical journal

authors： Miyamoto S,Maeda T,Fujime S

更新日期：1988-04-01 00:00:00

abstract：:The kinetics of porin incorporation into black lipid membranes (BLM) made of phosphatidylinositol (PI) or oxidized cholesterol (Ox Ch) were studied by means of alternating current; the set-up was able to acquire resistance and capacitance simultaneously by means of a mixed double-frequency approach at 1 Hz and 1 KHz, ...

journal_title：Biophysical journal

authors： Gallucci E,Micelli S,Monticelli G

更新日期：1996-08-01 00:00:00

abstract：:Glutamic acid [(L-glu)n] + dihydrogen phosphate systems are studied by infrared (IR) spectroscopy dried and hydrated at 75% relative humidity, as a function of both the phosphate-glutamic acid residue (Pi/glu) ratio and the type of cations present. It is shown that the glutamic acid groups form hydrogen-bonded chains ...

journal_title：Biophysical journal

authors： Burget U,Zundel G

更新日期：1987-12-01 00:00:00

abstract：:We have analyzed flash-induced period-four damped oscillation of oxygen evolution and chlorophyll fluorescence with the aid of a kinetic model of photosystem II. We have shown that, for simulation of the period-four oscillatory behavior of oxygen evolution, it is essential to consider the so-called intermediate S-stat...

journal_title：Biophysical journal

authors： Jablonsky J,Lazar D

更新日期：2008-04-01 00:00:00

abstract：:Here we present an efficient implementation of line-scan fluorescence correlation spectroscopy (i.e., one-dimensional spatio-temporal image correlation spectroscopy) using a commercial laser scanning microscope, which allows the accurate measurement of diffusion coefficients and concentrations in biological lipid memb...

journal_title：Biophysical journal

authors： Ries J,Chiantia S,Schwille P

更新日期：2009-03-04 00:00:00

abstract：:The group-additive decomposition of the unfolding free energy of a protein in an osmolyte solution relative to that in water poses a fundamental paradox: whereas the decomposition describes the experimental results rather well, theory suggests that a group-additive decomposition of free energies is, in general, not va...

journal_title：Biophysical journal

authors： Tomar DS,Asthagiri D,Weber V

更新日期：2013-09-17 00:00:00

abstract：:Structure factor amplitudes and phases can be computed directly from electron cryomicroscopy images. Inherent aberrations of the electromagnetic lenses and other instrumental factors affect the structure factors, however, resulting in decreased accuracy in the determined three-dimensional reconstruction. In contrast, ...

journal_title：Biophysical journal

authors： Thuman-Commike PA,Tsuruta H,Greene B,Prevelige PE Jr,King J,Chiu W

更新日期：1999-04-01 00:00:00

abstract：:The composition and electrolyte concentration of the aqueous bathing environment have important consequences for many biological processes and can profoundly affect the behavior of biomolecules. Nevertheless, because of computational limitations, many molecular simulations of biophysical systems can be performed only ...

journal_title：Biophysical journal

authors： Zavadlav J,Sablić J,Podgornik R,Praprotnik M

更新日期：2018-05-22 00:00:00

abstract：:Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and LOV2. LOV2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the FMN C(4a) atom. ...

journal_title：Biophysical journal

authors： Alexandre MT,Domratcheva T,Bonetti C,van Wilderen LJ,van Grondelle R,Groot ML,Hellingwerf KJ,Kennis JT

更新日期：2009-07-08 00:00:00

abstract：:Von Willebrand factor (VWF) is a multimeric plasma glycoprotein involved in both hemostasis and thrombosis. VWF conformational changes, especially unfolding of the A2 domain, may be required for efficient enzymatic cleavage in vivo. It has been shown that a single A2 domain unfolds at most probable unfolding forces of...

journal_title：Biophysical journal

authors： Ying J,Ling Y,Westfield LA,Sadler JE,Shao JY

更新日期：2010-04-21 00:00:00

abstract：:The Ca(2+) permeability of N-methyl-D-aspartate receptor (NMDA-R) channels was studied in human embryonic kidney cells transfected with the NR1-NR2A subunit combination. To determine the fractional Ca(2+) current (P(f)), measurements of fura-2-based Ca(2+) influx and whole-cell currents were made in symmetrical monova...

journal_title：Biophysical journal

authors： Schneggenburger R

更新日期：1996-05-01 00:00:00

abstract：:Vinculin activation plays a critical role in focal adhesion initiation and formation. In its native state, vinculin is in an autoinhibitory conformation in which domain 1 prevents interaction of the vinculin tail domain with actin by steric hindrance. Once activated, vinculin is able to interact with both actin and ta...

journal_title：Biophysical journal

authors： Golji J,Mofrad MR

更新日期：2010-08-09 00:00:00

abstract：:The x-ray structure analysis of photosystem I (PS I) crystals at 4-A resolution (Schubert et al., 1997, J. Mol. Biol. 272:741-769) has revealed the distances between the three iron-sulfur clusters, labeled F(X), F(1), and F(2), which function on the acceptor side of PS I. There is a general consensus concerning the as...

journal_title：Biophysical journal

authors： Shinkarev VP,Vassiliev IR,Golbeck JH

更新日期：2000-01-01 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:The (31)P-nuclear magnetic resonance chemical shift of phosphatidic acid in a membrane is sensitive to the lipid head group packing and can report qualitatively on membrane lateral compression near the aqueous interface. We have used high-resolution (31)P-nuclear magnetic resonance to evaluate the lateral compression ...

journal_title：Biophysical journal

authors： Traïkia M,Warschawski DE,Lambert O,Rigaud JL,Devaux PF

更新日期：2002-09-01 00:00:00

abstract：:DNA represents a promising therapeutic and prophylactic macromolecule in treating genetic diseases, infectious diseases and cancers. The therapeutic potential of DNA is directly related to how DNA transports within the targeted tissue. In this study, fluorescence photobleaching recovery was used to examine the diffusi...

journal_title：Biophysical journal

authors： Shen H,Hu Y,Saltzman WM

更新日期：2006-07-15 00:00:00

abstract：:The products of MgATP hydrolysis bind to the nucleotide site of myosin and thus may be expected to inhibit the contraction of muscle fibers. We measured the effects of phosphate and MgADP on the isometric tensions and isotonic contraction velocities of glycerinated rabbit psoas muscle at 10 degrees C. Addition of phos...

journal_title：Biophysical journal

authors： Cooke R,Pate E

更新日期：1985-11-01 00:00:00

abstract：:A partially depolarized squid axon membrane is assumed to have a quasi-steady state negative resistance, the membrane potential is clamped at one point, and a distribution of potential along the axon is obtained from the cable equation. Nominal experimental values of -2 ohm cm(2) for the membrane and 6 ohm cm(2) for t...

journal_title：Biophysical journal

authors： COLE KS

更新日期：1961-05-01 00:00:00

abstract：:Chimeras consisting of the homologous skeletal dihydropyridine receptor (DHPR) beta1a subunit and the heterologous cardiac/brain beta2a subunit were used to determine which regions of beta1a were responsible for the skeletal-type excitation-contraction (EC) coupling phenotype. Chimeras were transiently transfected in ...

journal_title：Biophysical journal

authors： Sheridan DC,Cheng W,Carbonneau L,Ahern CA,Coronado R

更新日期：2004-08-01 00:00:00

abstract：:The molecular weight of single-stranded DNA from the slime mold Physarum polycephalum has been determined by alkaline gradient centrifugation. The average molecular weight during DNA synthesis ( approximately 1.5 x 10(7) D) is less than that observed in nonsynthetic periods ( approximately 4 x 10(7) D). On the basis o...

journal_title：Biophysical journal

authors： McGrath RA,Williams RW

更新日期：1967-05-01 00:00:00

abstract：:We revisit a heteropolymer collapse theory originally introduced to explore how the balance between hydrophobic interactions and configurational entropy determines the thermal stability of globular proteins at ambient pressure. We generalize the theory by introducing a basic statistical mechanical treatment for how pr...

journal_title：Biophysical journal

authors： Cheung JK,Shah P,Truskett TM

更新日期：2006-10-01 00:00:00

abstract：:Actomyosin interactions were examined by using yeast actin mutants with alanines replacing charged amino acid pairs D24/D25, E99/E100, D80/D81, and E83/K84. In the in vitro motility experiments, actin filaments of D24A/D25A or E99A/E100A mutants moved in the presence of 0.7% methylcellulose at the velocities of wild-t...

journal_title：Biophysical journal

authors： Miller CJ,Cheung P,White P,Reisler E

更新日期：1995-04-01 00:00:00

abstract：:Two-dimensional 1H NMR spectroscopy over a range of temperature through thermal unfolding has been applied to the low-spin, ferric cyanide complex of myoglobin from Aplysia limacina to search for intermediates in the unfolding and to characterize the effect of temperature on the magnetic properties and electronic stru...

journal_title：Biophysical journal

authors： Xia Z,Nguyen BD,Brunori M,Cutruzzolà F,La Mar GN

更新日期：2005-12-01 00:00:00

abstract：:Gangliosides are a class of glycosphingolipids (GSLs) with amphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domains makes them vital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustere...

journal_title：Biophysical journal

authors： Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

更新日期：2016-11-01 00:00:00

abstract：:The structure of polymer-decorated phospholipid monolayers at the solid-solution interface was investigated using neutron reflectometry. The monolayers were composed of distearoylphosphatidylethanolamine (DSPE) matrixed with varying amounts of DSPE-PEG (DSPE with polyethylene glycol covalently grafted to its headgroup...

journal_title：Biophysical journal

authors： Kuhl TL,Majewski J,Wong JY,Steinberg S,Leckband DE,Israelachvili JN,Smith GS

更新日期：1998-11-01 00:00:00