Attraction of rotors to the pulmonary veins in paroxysmal atrial fibrillation: a modeling study.

abstract：:Many essential processes in eukaryotic cells depend on regulated molecular exchange between its two major compartments, the cytoplasm and the nucleus. In general, nuclear import of macromolecular complexes is dependent on specific peptide signals and their recognition by receptors that mediate translocation through th...

journal_title：Biophysical journal

authors： Salman H,Abu-Arish A,Oliel S,Loyter A,Klafter J,Granek R,Elbaum M

更新日期：2005-09-01 00:00:00

abstract：:Chimeras consisting of the homologous skeletal dihydropyridine receptor (DHPR) beta1a subunit and the heterologous cardiac/brain beta2a subunit were used to determine which regions of beta1a were responsible for the skeletal-type excitation-contraction (EC) coupling phenotype. Chimeras were transiently transfected in ...

journal_title：Biophysical journal

authors： Sheridan DC,Cheng W,Carbonneau L,Ahern CA,Coronado R

更新日期：2004-08-01 00:00:00

abstract：:The effect of antibodies to glycophorin A on the rotational diffusion of band 3 in human erythrocyte membranes was investigated by transient dichrosim. Three antibodies that recognize different epitopes on the exofacial domain of glycophorin A all strongly reduce the rotational mobility of band 3. The effect is at mos...

journal_title：Biophysical journal

authors： Che A,Cherry RJ

更新日期：1995-05-01 00:00:00

abstract：:We have studied the large increase in macroscopic potassium channel current caused by catecholamines in mammalian cardiac cells. An increase in macroscopic K current could result from either an increase in the single-channel current or by an increase in the number of channels that are open. Therefore, we have measured...

journal_title：Biophysical journal

authors： Bennett PB,Kass R,Begenisich T

更新日期：1989-04-01 00:00:00

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:The vessel wall experiences progressive stiffening with age and the development of cardiovascular disease, which alters the micromechanical environment experienced by resident vascular smooth muscle cells (VSMCs). In vitro studies have shown that VSMCs are sensitive to substrate stiffness, but the exact molecular mech...

journal_title：Biophysical journal

authors： Sazonova OV,Lee KL,Isenberg BC,Rich CB,Nugent MA,Wong JY

更新日期：2011-08-03 00:00:00

abstract：:Native cGMP-gated channels were studied in rod outer segments of the larval tiger salamander Ambystoma tigrinum. The alpha subunit of the cGMP-gated channel from bovine rods, here referred to as the wild type (w.t.), and mutant channels were heterologously expressed in Xenopus laevis oocytes. These channels were studi...

journal_title：Biophysical journal

authors： Sesti F,Nizzari M,Torre V

更新日期：1996-06-01 00:00:00

abstract：:Pacemaker depolarization in interstitial cells of Cajal (ICCs) is believed to be induced by Ca2+ transients and activation of anoctamin-1 (Ano1) channels in the plasma membrane. However, block of store-operated calcium entry (SOCE) or the Na-K-2Cl cotransporter (NKCC1) terminates pacemaker activity in ICC, indicating ...

journal_title：Biophysical journal

authors： Youm JB,Zheng H,Koh SD,Sanders KM

更新日期：2019-08-20 00:00:00

abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...

journal_title：Biophysical journal

authors： Butler WL

更新日期：1972-07-01 00:00:00

abstract：:The structural features of SPM that control the transbilayer distribution of beta-GalCer in POPC vesicles were investigated by (13)C- and (31)P-NMR spectroscopy using lipid analogs that share physical similarities with GalCer or SPM. The SPM analogs included N-palmitoyl-4,5-dihydro-SPM, 3-deoxy-SPM, 1-alkyl-2-amidopho...

journal_title：Biophysical journal

authors： Malewicz B,Valiyaveettil JT,Jacob K,Byun HS,Mattjus P,Baumann WJ,Bittman R,Brown RE

更新日期：2005-04-01 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:We present a new approach to probing single-particle dynamics that uses dynamic light scattering from a localized region. By scattering a focused laser beam from a micron-size particle, we measure its spatial fluctuations via the temporal autocorrelation of the scattered intensity. We demonstrate the applicability of ...

journal_title：Biophysical journal

authors： Meller A,Bar-Ziv R,Tlusty T,Moses E,Stavans J,Safran SA

更新日期：1998-03-01 00:00:00

abstract：:2D-ELDOR spectroscopy has been employed to study the dynamic structure of the liquid-ordered (Lo) phase versus that of the liquid-crystalline (Lc) phase in multibilayer phospholipid vesicles without (Lc) and with (Lo) cholesterol, using end-chain and headgroup labels and spin-labeled cholestane. The spectra are in mos...

journal_title：Biophysical journal

authors： Costa-Filho AJ,Shimoyama Y,Freed JH

更新日期：2003-04-01 00:00:00

abstract：:Thymine dimers were irradiated in aqueous solution with (60)Co gamma-rays in N(2) or O(2). Thymine and unidentified non-UV-absorbing products appeared. The thymine was identified by spectrophotometry, chromatography, and ability to support the growth of Escherichia coli 15 T(-). Residual dimer was determined by a UV-r...

journal_title：Biophysical journal

authors： Deering RA,Snipes W

更新日期：1968-03-01 00:00:00

abstract：:In the first issue, on the first page of the Biophysical Journal in 1960, Cole and Moore provided the first confirmation of the Hodgkin and Huxley formulation of the sodium and potassium conductances that underlie the action potential. In addition, working with the squid giant axon, Cole and Moore noted that strong hy...

journal_title：Biophysical journal

authors： Hoshi T,Armstrong CM

更新日期：2015-10-06 00:00:00

abstract：:The effect of agarose on nucleation of hen egg white lysozyme crystal was examined quantitatively using a temperature-jumping technique. For the first time, to our knowledge, the inhibition of agarose during the nucleation of lysozyme was quantified in two respects: a), the effect of increasing interfacial nucleation ...

journal_title：Biophysical journal

authors： Wang L,Liu XY

更新日期：2008-12-15 00:00:00

abstract：:A complete mechanistic understanding of membrane-localized processes in vesicular transport, such as membrane budding and fission, requires their reconstitution with biochemically-defined components from a biochemically-defined substrate. Supported bilayers formed by vesicle fusion represent an attractive substrate fo...

journal_title：Biophysical journal

authors： Pucadyil TJ,Schmid SL

更新日期：2010-07-21 00:00:00

abstract：:In the photocycle of bacteriorhodopsin at pH 7, a proton is ejected to the extracellular medium during the protonation of Asp-85 upon formation of the M intermediate. The group that releases the ejected proton does not become reprotonated until the prephotolysis state is restored from the N and O intermediates. In con...

journal_title：Biophysical journal

authors： Morgan JE,Vakkasoglu AS,Lanyi JK,Lugtenburg J,Gennis RB,Maeda A

更新日期：2012-08-08 00:00:00

abstract：:Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles o...

journal_title：Biophysical journal

authors： Ramadass R,Bereiter-Hahn J

更新日期：2008-10-01 00:00:00

abstract：:Adsorption-desorption isotherms were obtained for water binding by 1,2-dimyristoylphosphatidylcholine in the temperature range 15 degrees-35 degrees C. The isotherms were analyzed by Brunauer et al.'s (BET) theory and also a polarization theory, the latter being more successful in fitting the data. There was some evid...

journal_title：Biophysical journal

authors： Wilkinson DA,Morowitz HJ,Prestegard JH

更新日期：1977-11-01 00:00:00

abstract：:A model alpha-helical peptide encapsulated in a reverse micelle is used to study the structure and dynamics of proteins under constrained environments that mimic the membrane-water environment in cells. Molecular dynamics simulations of the self assembly of systems composed of a peptide, sodium bis(2-ethylhexyl) sulfo...

journal_title：Biophysical journal

authors： Tian J,Garcia AE

更新日期：2009-05-20 00:00:00

abstract：:Flow dialysis has found widespread use in determining the dissociation constant (KD) of a protein-ligand interaction or the amount of available binding sites (E0). This method has the potency to measure both these parameters in a single experiment and in this article a method to measure simultaneously the KD and E0 is...

journal_title：Biophysical journal

authors： Veldhuis G,Vos EP,Broos J,Poolman B,Scheek RM

更新日期：2004-04-01 00:00:00

abstract：:Lipid droplets (LDs) are cytosolic organelles that protrude from the endoplasmic reticulum membrane under energy-rich conditions. How an LD buds off from the endoplasmic reticulum bilayer is still elusive. By using a continuous media description, we computed the morphology of a lipid droplet embedded in between two id...

journal_title：Biophysical journal

authors： Deslandes F,Thiam AR,Forêt L

更新日期：2017-07-11 00:00:00

abstract：:In ubiquitination, cullin-RING E3 ubiquitin ligases (CRLs) assist in ubiquitin transfer from ubiquitin-conjugating enzyme E2 to the substrate. Neddylation, which involves NEDD8 transfer from E2 to E3-cullin, stimulates ubiquitination by inducing conformational change in CRLs. However, deneddylation, which removes NEDD...

journal_title：Biophysical journal

authors： Liu J,Nussinov R

更新日期：2010-08-04 00:00:00

abstract：:We present a combined experimental and computational study of the bundling of F-actin filaments induced by lysozyme proteins. Synchrotron small-angle x-ray scattering results show that these bundles consist of close-packed columnar complexes in which the actin is held together by incommensurate, one-dimensional arrays...

journal_title：Biophysical journal

authors： Guáqueta C,Sanders LK,Wong GC,Luijten E

更新日期：2006-06-15 00:00:00

abstract：:Elastin-derived peptides are released from the extracellular matrix remodeling by numerous proteases and seem to regulate many biological processes, notably cancer progression. The canonical elastin peptide is VGVAPG, which harbors the XGXXPG consensus pattern, allowing interaction with the elastin receptor complex lo...

journal_title：Biophysical journal

authors： Hernández B,Crowet JM,Thiery J,Kruglik SG,Belloy N,Baud S,Dauchez M,Debelle L

更新日期：2020-06-02 00:00:00

abstract：:The interaction of cells in a tissue depends on the nature of the extracellular matrix. The electrical properties of the narrow extracellular space are unknown. Here we consider cell adhesion mediated by extracellular matrix protein on a solid substrate as a model system. We culture human embryonic kidney (HEK293) cel...

journal_title：Biophysical journal

authors： Gleixner R,Fromherz P

更新日期：2006-04-01 00:00:00

abstract：:Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. Application of the dual whole-cell voltage clamp technique with patch electrodes to pairs of transfected SKHep1 cells revealed strong sensit...

journal_title：Biophysical journal

authors： Moreno AP,Eghbali B,Spray DC

更新日期：1991-11-01 00:00:00

abstract：:The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH in...

journal_title：Biophysical journal

authors： Wang D,Kreutzer U,Chung Y,Jue T

更新日期：1997-11-01 00:00:00

abstract：:We describe a model that relates the maximum shortening velocity of a muscle fiber, Vm, to the kinetics of the dissociation of a myosin head from actin. At Vm, the positive work exerted by cross-bridges attached in the powerstroke must be balanced by cross-bridges that have been carried by movement of the filaments in...

journal_title：Biophysical journal

authors： Cooke R,White H,Pate E

更新日期：1994-03-01 00:00:00