Abstract:
:Many essential processes in eukaryotic cells depend on regulated molecular exchange between its two major compartments, the cytoplasm and the nucleus. In general, nuclear import of macromolecular complexes is dependent on specific peptide signals and their recognition by receptors that mediate translocation through the nuclear pores. Here we address the question of how protein products bearing such nuclear localization signals arrive at the nuclear membrane before import, i.e., by simple diffusion or perhaps with assistance of cytoskeletal elements or cytoskeleton-associated motor proteins. Using direct single-particle tracking and detailed statistical analysis, we show that the presence of nuclear localization signals invokes active transport along microtubules in a cell-free Xenopus egg extract. Chemical and antibody inhibition of minus-end directed cytoplasmic dynein blocks this active movement. In the intact cell, where microtubules project radially from the centrosome, such an interaction would effectively deliver nuclear-targeted cargo to the nuclear envelope in preparation for import.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Salman H,Abu-Arish A,Oliel S,Loyter A,Klafter J,Granek R,Elbaum Mdoi
10.1529/biophysj.105.060160subject
Has Abstractpub_date
2005-09-01 00:00:00pages
2134-45issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(05)72857-5journal_volume
89pub_type
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