Abstract:
:The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical limits on the imaging field of view size. Here, we present a light sheet fluorescence microscope that achieves 390 nm isotropic resolution and high optical sectioning strength (i.e., out-of-focus blur is strongly suppressed) over large field of views, without the need for structured illumination or deconvolution-based postprocessing. We demonstrate simultaneous dual-color, high-contrast, and high-dynamic-range time-lapse imaging of migrating cells in complex three-dimensional microenvironments, three-dimensional tracking of clathrin-coated pits, and long-term imaging spanning >10 h and encompassing >2600 time points.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Dean KM,Roudot P,Welf ES,Danuser G,Fiolka Rdoi
10.1016/j.bpj.2015.05.013subject
Has Abstractpub_date
2015-06-16 00:00:00pages
2807-15issue
12eissn
0006-3495issn
1542-0086pii
S0006-3495(15)00498-1journal_volume
108pub_type
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