Abstract:
:The cellular response to external mechanical forces has important effects on numerous biological phenomena. The sequences of molecular events that underlie the observed changes in cellular properties have yet to be elucidated in detail. Here we have detected the responses of a cultured cell against locally applied cyclic stretching and compressive forces, after creating an artificial focal adhesion under a glass bead attached to the cantilever of an atomic force microscope. The cell tension initially increased in response to the tensile stress and then decreased within ∼1 min as a result of viscoelastic properties of the cell. This relaxation was followed by a gradual increase in tension extending over several minutes. The slow recovery of tension ceased after several cycles of force application. This tension-recovering activity was inhibited when cells were treated with cytochalasin D, an inhibitor of actin polymerization, or with (-)-blebbistatin, an inhibitor of myosin II ATPase activity, suggesting that the activity was driven by actin-myosin interaction. To our knowledge, this is the first quantitative analysis of cellular mechanical properties during the process of adaptation to locally applied cyclic external force.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Watanabe-Nakayama T,Machida SI,Harada I,Sekiguchi H,Afrin R,Ikai Adoi
10.1016/j.bpj.2010.12.3693subject
Has Abstractpub_date
2011-02-02 00:00:00pages
564-572issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(10)05208-2journal_volume
100pub_type
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