The Photosystem II Subunit S under Stress.

abstract：:The freshwater polyp Hydra can regenerate from tissue fragments or random cell aggregates. We show that the axis-defining step ("symmetry breaking") of regeneration requires mechanical inflation-collapse oscillations of the initial cell ball. We present experimental evidence that axis definition is retarded if these o...

journal_title：Biophysical journal

authors： Soriano J,Rüdiger S,Pullarkat P,Ott A

更新日期：2009-02-18 00:00:00

abstract：:Coarse-grained (CG) models of biomolecules have recently attracted considerable interest because they enable the simulation of complex biological systems on length-scales and timescales that are inaccessible for atomistic molecular dynamics simulation. A CG model is defined by a map that transforms an atomically detai...

journal_title：Biophysical journal

authors： Zhang Z,Lu L,Noid WG,Krishna V,Pfaendtner J,Voth GA

更新日期：2008-12-01 00:00:00

abstract：:We used micron-sized latex spheres to probe the phase state and the viscoelastic properties of dimyristoylphosphatidylcholine (DMPC) bilayers as a function of temperature. One or two particles were manipulated and stuck to a DMPC giant vesicle by means of an optical trap. Above the fluid-gel main transition temperatur...

journal_title：Biophysical journal

authors： Dimova R,Pouligny B,Dietrich C

更新日期：2000-07-01 00:00:00

abstract：:A number of membrane proteins act via binding at the water/lipid bilayer interface. An important example of such proteins is provided by the gating-modifier toxins that act on voltage-gated potassium (Kv) channels. They are thought to partition to the headgroup region of lipid bilayers, and so provide a good system fo...

journal_title：Biophysical journal

authors： Wee CL,Gavaghan D,Sansom MS

更新日期：2008-10-01 00:00:00

abstract：:Femtosecond time-resolved transient absorption spectroscopy was employed to characterize for the first time the primary photoisomerization dynamics of a bacterial phytochrome system in the two thermally stable states of the photocycle. The 85-kDa phytochrome Cph1 from the cyanobacterium Synechocystis PCC 6803 expresse...

journal_title：Biophysical journal

authors： Heyne K,Herbst J,Stehlik D,Esteban B,Lamparter T,Hughes J,Diller R

更新日期：2002-02-01 00:00:00

abstract：:Herein, we identify the coordination environment of Cu²(+) in the human α1-glycine receptor (GlyR). GlyRs are members of the pentameric ligand-gated ion channel superfamily (pLGIC) that mediate fast signaling at synapses. Metal ions like Zn²(+) and Cu²(+) significantly modulate the activity of pLGICs, and metal ion co...

journal_title：Biophysical journal

authors： Ruthstein S,Stone KM,Cunningham TF,Ji M,Cascio M,Saxena S

更新日期：2010-10-20 00:00:00

abstract：:We present the measurement of the force required to rupture a single protein-sugar bond using a methodology that provides selective discrimination between specific and nonspecific binding events and helps verify the presence of a single functional molecule on the atomic force microscopy tip. In particular, the interac...

journal_title：Biophysical journal

authors： Ratto TV,Langry KC,Rudd RE,Balhorn RL,Allen MJ,McElfresh MW

更新日期：2004-04-01 00:00:00

abstract：:Coarse-grained molecular dynamics simulations are applied to explore the experimentally observed ability of the liquid-ordered (lo)/liquid-disordered (ld) phase boundary to facilitate viral membrane fusion. Surprisingly, a formed fusion stalk can be both attracted (i.e., stalkophilic) and repelled (i.e., stalkophobic)...

journal_title：Biophysical journal

authors： Risselada HJ

更新日期：2017-06-20 00:00:00

abstract：:N-BAR domains are protein modules that bind to and induce curvature in membranes via a charged concave surface and N-terminal amphipathic helices. Recently, molecular dynamics simulations have demonstrated that the N-BAR domain can induce a strong local curvature that matches the curvature of the BAR domain surface fa...

journal_title：Biophysical journal

authors： Blood PD,Swenson RD,Voth GA

更新日期：2008-08-01 00:00:00

abstract：:Phosphatidylinositol phospholipase Cγ (PLCγ) is an intracellular membrane-associated second-messenger signaling protein activated by tyrosine kinases such as fibroblast growth factor receptor 1. PLCγ contains the regulatory γ-specific array (γSA) comprising a tandem Src homology 2 (SH2) pair, an SH3 domain, and a spli...

journal_title：Biophysical journal

authors： Koss H,Bunney TD,Esposito D,Martins M,Katan M,Driscoll PC

更新日期：2018-07-03 00:00:00

abstract：:The initial output of a time-resolved macromolecular crystallography experiment is a time-dependent series of difference electron density maps that displays the time-dependent changes in underlying structure as a reaction progresses. The goal is to interpret such data in terms of a small number of crystallographically...

journal_title：Biophysical journal

authors： Kostov KS,Moffat K

更新日期：2011-01-19 00:00:00

abstract：:The formulation of Lorente de Nó for the potential distribution in a volume conductor due to a contained bioelectric source is reviewed. The aforementioned formula is based on data taken from the bioelectric source in air (excised). This work is extended to the derivation of a formula which depends upon quantities ava...

journal_title：Biophysical journal

authors： PLONSEY R

更新日期：1964-07-01 00:00:00

abstract：:We have examined the morphology and osmotic properties of large unilamellar vesicles (LUVs) prepared by extrusion. Contrary to expectations, we observe by cryo-electron microscopy that such vesicles, under isoosmotic conditions, are non-spherical. This morphology appears to be a consequence of vesicle passage through ...

journal_title：Biophysical journal

authors： Mui BL,Cullis PR,Evans EA,Madden TD

更新日期：1993-02-01 00:00:00

abstract：:TWIK-1 two-pore domain K(+) channels generally produce nonmeasurable or very low levels of K(+) currents in heterologous expression systems under physiologically ionic conditions. Two controversial mechanisms have been proposed to account for this behavior: TWIK-1 K(+) channels are expressed in the cell surface but si...

journal_title：Biophysical journal

authors： Ma L,Xie YP,Zhou M,Chen H

更新日期：2012-04-18 00:00:00

abstract：:Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin g...

journal_title：Biophysical journal

authors： Horn C,Steinem C

更新日期：2005-08-01 00:00:00

abstract：:The bovine eye lens protein, alpha L crystallin, has been studied with photon correlation spectroscopy and statical light scattering in the concentration range up to 200 g/l in different solvent conditions. At higher concentration (c greater than 70 g/l) the scattering behavior is quite complicated, which results in n...

journal_title：Biophysical journal

authors： Andries C,Clauwaert J

更新日期：1985-05-01 00:00:00

abstract：:Actin is ubiquitous globular protein that polymerizes into filaments and forms networks that participate in the force generation of eukaryotic cells. Such forces are used for cell motility, cytokinesis, and tissue remodeling. Among those actin networks, we focus on the actin cortex, a dense branched network beneath th...

journal_title：Biophysical journal

authors： Bussonnier M,Carvalho K,Lemière J,Joanny JF,Sykes C,Betz T

更新日期：2014-08-19 00:00:00

abstract：:In addition to being the gateway for all access to the eukaryotic genome, chromatin has in recent years been identified as carrying an epigenetic code regulating transcriptional activity. Though much is known about the biochemistry of this code, little is understood regarding the different fiber structures through whi...

journal_title：Biophysical journal

authors： Depken M,Schiessel H

更新日期：2009-02-01 00:00:00

abstract：:The effects of different cations on the hydrodynamic radius (RH) of a 48-bp synthetic DNA are measured by time-resolved fluorescence polarization anisotropy of intercalated ethidium. Relative statistical errors in RH are only approximately 1%. With increasing cation concentration, Na+ causes a small decrease in RH, Cs...

journal_title：Biophysical journal

authors： Fujimoto BS,Miller JM,Ribeiro NS,Schurr JM

更新日期：1994-07-01 00:00:00

abstract：:An assumption usually made when developing kinetic models for the gating of ion channels is that the transitions among the various states involved in the gating obey microscopic reversibility. If this assumption is incorrect, then the models and estimated rate constants made with the assumption would be in error. This...

journal_title：Biophysical journal

authors： Song L,Magleby KL

更新日期：1994-07-01 00:00:00

abstract：:VP16, a protein encoded by herpes simplex virus, has a well-characterized 78 amino acid acidic activation domain. When tethered to DNA, tandem repeats of an eight amino acid motif taken from this region stimulate the transcription of a nearby gene. This work addresses how these minimal activation motifs interact with ...

journal_title：Biophysical journal

authors： Bamdad C

更新日期：1998-10-01 00:00:00

abstract：:Leukocyte capture and rolling on the vascular endothelium is mediated principally by the selectin family of cell adhesion receptors. In a parallel plate flow chamber, neutrophil rolling on purified selectins or a selectin-ligand substrate was resolved by high speed videomicroscopy as a series of ratchet-like steps wit...

journal_title：Biophysical journal

authors： Smith MJ,Berg EL,Lawrence MB

更新日期：1999-12-01 00:00:00

abstract：:Cryo-electron microscopy is rapidly emerging as a powerful technique to determine the structures of complex macromolecular systems elusive to other techniques. Because many of these systems are highly dynamical, characterizing their movements is also a crucial step to unravel their biological functions. To achieve thi...

journal_title：Biophysical journal

authors： Bonomi M,Pellarin R,Vendruscolo M

更新日期：2018-04-10 00:00:00

abstract：:To investigate the mechanisms by which vesicles of pulmonary surfactant adsorb to an air-liquid interface, we measured the effect of different phospholipids and of their concentration both in the subphase and at the interface on this process. Adsorbing vesicles contained the hydrophobic surfactant proteins mixed with ...

journal_title：Biophysical journal

authors： Walters RW,Jenq RR,Hall SB

更新日期：2000-01-01 00:00:00

abstract：:Antisense oligonucleotides present a powerful means to inhibit expression of specific genes, but their effectiveness is limited by factors including cellular delivery, biochemical attack, and poor binding to target. We have developed a systems model of the processes required for an antisense oligonucleotide to enter, ...

journal_title：Biophysical journal

authors： Roth CM

更新日期：2005-10-01 00:00:00

abstract：:Total internal reflection (TIR) microfluorimetry was established as a method to measure continuously the volume of adherent cells and applied to measure membrane permeabilities in cells transfected with water channel homologs. Cytosol was labeled with the membrane-impermeant fluorophore calcein. Fluorescence was excit...

journal_title：Biophysical journal

authors： Farinas J,Simanek V,Verkman AS

更新日期：1995-04-01 00:00:00

abstract：:Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

journal_title：Biophysical journal

authors： De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

更新日期：2010-09-22 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spec...

journal_title：Biophysical journal

authors： Apte DV,Koutalos Y,McFarlane DK,Dawson MJ,Ebrey TG

更新日期：1989-09-01 00:00:00

abstract：:The structure of fully hydrated gel phase dimyristoylphosphatidylcholine lipid bilayers was obtained at 10 degrees C. Oriented lipid multilayers were used to obtain high signal-to-noise intensity data. The chain tilt angle and an estimate of the methylene electron density were obtained from wide angle reflections. The...

journal_title：Biophysical journal

authors： Tristram-Nagle S,Liu Y,Legleiter J,Nagle JF

更新日期：2002-12-01 00:00:00

abstract：:Video-based particle tracking monitors the microscopic movement of labeled biomolecules and fluorescent probes within a complex cellular environment. Information gained from this technique enables us to extract the dynamic behavior of biomolecules and the local mechanical properties inside the cell from a tracked part...

journal_title：Biophysical journal

authors： Wu PH,Arce SH,Burney PR,Tseng Y

更新日期：2009-06-17 00:00:00