Abstract:
:TWIK-1 two-pore domain K(+) channels generally produce nonmeasurable or very low levels of K(+) currents in heterologous expression systems under physiologically ionic conditions. Two controversial mechanisms have been proposed to account for this behavior: TWIK-1 K(+) channels are expressed in the cell surface but silenced by sumoylation at a lysine residue (TWIK-1 K274); constitutive and rapid internalization of TWIK-1 causes TWIK-1 channel silencing. Here we report that TWIK-1 K(+) channels heterologously expressed in Chinese hamster ovary cells, which are silent in physiological K(+) gradients, are able to conduct large monovalent cation currents when extracellular ionic conditions change. These results support the hypothesis that TWIK-1 K(+) channels are expressed in the cell surface but silent, and suggest that the TWIK-1 gating behavior rather than the lack of cell surface expression of TWIK-1 results in nondetectable TWIK-1 K(+) currents in heterologous expression systems.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Ma L,Xie YP,Zhou M,Chen Hdoi
10.1016/j.bpj.2012.03.011subject
Has Abstractpub_date
2012-04-18 00:00:00pages
L34-6issue
8eissn
0006-3495issn
1542-0086pii
S0006-3495(12)00321-9journal_volume
102pub_type
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