Abstract:
:Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Apte DV,Koutalos Y,McFarlane DK,Dawson MJ,Ebrey TGdoi
10.1016/S0006-3495(89)82691-8subject
Has Abstractpub_date
1989-09-01 00:00:00pages
447-52issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(89)82691-8journal_volume
56pub_type
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