A novel approach to high accuracy of video-based microrheology.


:Video-based particle tracking monitors the microscopic movement of labeled biomolecules and fluorescent probes within a complex cellular environment. Information gained from this technique enables us to extract the dynamic behavior of biomolecules and the local mechanical properties inside the cell from a tracked particle's mean-square displacement (MSD). However, MSD measurements are highly susceptible to static error introduced by noise in the image acquisition process that leads to an incorrect positioning of the particle. Static error can mask the subtle effects from the local microenvironment on the MSD and potentially generate misleading conclusions about the biophysical properties of cells. An approach that greatly increases the accuracy of MSD measurements is presented herein by combining experimental data with Monte Carlo simulations to eliminate the inherent static error. This practical method of static error correction greatly advances particle-tracking techniques.


Biophys J


Biophysical journal


Wu PH,Arce SH,Burney PR,Tseng Y




Has Abstract


2009-06-17 00:00:00














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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Rezania V,Azarenko O,Jordan MA,Bolterauer H,Ludueña RF,Huzil JT,Tuszynski JA

    更新日期:2008-08-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sergeev M,Costantino S,Wiseman PW

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  • Structural locus of the pH gate in the Kir1.1 inward rectifier channel.

    abstract::The closed-state crystal structure of prokaryotic inward rectifier, KirBac1.1, has implicated four inner helical phenylalanines near the cytoplasmic side as a possible locus of the channel gate. In the present study, we investigate whether this structural feature corresponds to the physiological pH gate of the renal i...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sackin H,Nanazashvili M,Palmer LG,Krambis M,Walters DE

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Owen DM,Lanigan PM,Dunsby C,Munro I,Grant D,Neil MA,French PM,Magee AI

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sengupta K,Aranda-Espinoza H,Smith L,Janmey P,Hammer D

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Suzuki K,Sterba RE,Sheetz MP

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    journal_title:Biophysical journal

    pub_type: 杂志文章


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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ostuni A,Bochicchio B,Armentano MF,Bisaccia F,Tamburro AM

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  • The binomial model in fluctuation analysis of quantal neurotransmitter release.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Quastel DM

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  • Modeling the attenuation and failure of action potentials in the dendrites of hippocampal neurons.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Migliore M

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  • Conformational dynamics of bovine Cu, Zn superoxide dismutase revealed by time-resolved fluorescence spectroscopy of the single tyrosine residue.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ferreira ST,Stella L,Gratton E

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  • Na-K-2Cl Cotransporter and Store-Operated Ca2+ Entry in Pacemaking by Interstitial Cells of Cajal.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Bieler J,Pozzorini C,Naef F

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    journal_title:Biophysical journal

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    authors: Dennison SM,Bowen ME,Brunger AT,Lentz BR

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sukharev S

    更新日期:2002-07-01 00:00:00

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    pub_type: 杂志文章


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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vosegaard T,Kamihira-Ishijima M,Watts A,Nielsen NC

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  • Protein-induced membrane curvature investigated through molecular dynamics flexible fitting.

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  • Photochemically induced charge separation occurring in bacteriorhodopsin. Detection by time-resolved dielectric loss.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


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  • Molecular simulation of the binding of nerve growth factor peptide mimics to the receptor tyrosine kinase A.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Berrera M,Cattaneo A,Carloni P

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  • Origin of axon membrane hyperpolarization under sucrose-gap.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


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  • Lipid Fingerprints and Cofactor Dynamics of Light-Harvesting Complex II in Different Membranes.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Thallmair S,Vainikka PA,Marrink SJ

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  • Accurate prediction of gene expression by integration of DNA sequence statistics with detailed modeling of transcription regulation.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vilar JM

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Prajapat MK,Jain K,Saini S

    更新日期:2015-10-06 00:00:00

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    abstract::GABA and glycine receptors (GlyRs) are pentameric ligand-gated ion channels that respond to the inhibitory neurotransmitters by opening a chloride-selective central pore lined with five M2 segments homologous to those of alpha(1) GlyR/ ARVG(2')LGIT(6')TVLTMTTQSSGSR. The activity of cyanotriphenylborate (CTB) and picro...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Zhorov BS,Bregestovski PD

    更新日期:2000-04-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Mikaelsson T,Adén J,Johansson LB,Wittung-Stafshede P

    更新日期:2013-02-05 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Nag A,Dinner AR

    更新日期:2006-02-01 00:00:00

  • Determination of membrane-insertion free energies by molecular dynamics simulations.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gumbart J,Roux B

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Famili I,Palsson BO

    更新日期:2003-07-01 00:00:00

  • Two-photon cross-correlation analysis of intracellular reactions with variable stoichiometry.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kim SA,Heinze KG,Bacia K,Waxham MN,Schwille P

    更新日期:2005-06-01 00:00:00