A novel approach to high accuracy of video-based microrheology.

Abstract:

:Video-based particle tracking monitors the microscopic movement of labeled biomolecules and fluorescent probes within a complex cellular environment. Information gained from this technique enables us to extract the dynamic behavior of biomolecules and the local mechanical properties inside the cell from a tracked particle's mean-square displacement (MSD). However, MSD measurements are highly susceptible to static error introduced by noise in the image acquisition process that leads to an incorrect positioning of the particle. Static error can mask the subtle effects from the local microenvironment on the MSD and potentially generate misleading conclusions about the biophysical properties of cells. An approach that greatly increases the accuracy of MSD measurements is presented herein by combining experimental data with Monte Carlo simulations to eliminate the inherent static error. This practical method of static error correction greatly advances particle-tracking techniques.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Wu PH,Arce SH,Burney PR,Tseng Y

doi

10.1016/j.bpj.2009.03.029

subject

Has Abstract

pub_date

2009-06-17 00:00:00

pages

5103-11

issue

12

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(09)00770-X

journal_volume

96

pub_type

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