The polymer brush model of neurofilament projections: effect of protein composition.

abstract：:Forces generated by microtubule polymerization and depolymerization are important for the biological functioning of cells. The mean growth velocity, V, under an opposing force, F, has been measured by; Science 278:856-860) for single microtubules growing in vitro, but their analysis of the data suggested that V decrea...

journal_title：Biophysical journal

authors： Kolomeisky AB,Fisher ME

更新日期：2001-01-01 00:00:00

abstract：:The geminate recombination kinetics of CO-myoglobin strongly deviates from single exponential behavior in contrast to what is expected for unimolecular reactions (1). At low temperatures, this result was attributed to slowly exchanging conformational states which differ substantially in barrier height for ligand bindi...

journal_title：Biophysical journal

authors： Post F,Doster W,Karvounis G,Settles M

更新日期：1993-06-01 00:00:00

abstract：:Myoglobin (Mb) is a model system for ligand binding and migration. The energy barriers (ΔG) for ligand migration in Mb have been studied in the past by experiment and theory and significant differences between different approaches were found. From experiment, it is known that Mb can assume a large number of conformati...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2012-01-18 00:00:00

abstract：:Crystals have been grown of "sperm whale" myoglobin produced in Escherichia coli from a synthetic gene and the structure has been solved to 1.9 A resolution. Because of a remaining initiator methionine, this protein crystallizes in a different space group from native sperm whale myoglobin. The three-dimensional struct...

journal_title：Biophysical journal

authors： Phillips GN Jr

更新日期：1990-02-01 00:00:00

abstract：:Laurdan fluorescence, novel spectral fitting, and dynamic light scattering were combined to determine lateral lipid organization in mixed lipid membranes of the oxidized lipid, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), and each of the three bilayer lipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (...

journal_title：Biophysical journal

authors： Ranganathan R,Alshammri I,Peric M

更新日期：2020-04-21 00:00:00

abstract：:We present a three-dimensional tracking routine for nondiffraction-limited particles, which significantly reduces pixel bias. Our technique allows for increased resolution compared to that of previous methods, especially at low magnification or at high signal/noise ratio. This enables tracking with nanometer accuracy ...

journal_title：Biophysical journal

authors： van Loenhout MT,Kerssemakers JW,De Vlaminck I,Dekker C

更新日期：2012-05-16 00:00:00

abstract：:Are protein nonpolar cavities filled with water molecules? Although many experimental and theoretical investigations have been done, particularly for the nonpolar cavity of IL-1 beta, the results are still conflicting. To study this problem from the thermodynamic point of view, we calculated hydration free energies of...

journal_title：Biophysical journal

authors： Oikawa M,Yonetani Y

更新日期：2010-06-16 00:00:00

abstract：:A cell plated on a two-dimensional substrate forms adhesions with that surface. These adhesions, which consist of aggregates of various proteins, are thought to be important in mechanosensation, the process by which the cell senses and responds to the mechanical properties of the substrate (e.g., stiffness). On the ba...

journal_title：Biophysical journal

authors： Walcott S,Kim DH,Wirtz D,Sun SX

更新日期：2011-12-21 00:00:00

abstract：:A recently developed technique for in vivo determination of the electrical resistance of vascular endothelium in microvessels was applied to the vessels in a thin frog muscle, m. cutaneus pectoris. The technique consists of injection of current via a glass micropipette into a capillary and measurement of the resulting...

journal_title：Biophysical journal

authors： Olesen SP,Crone C

更新日期：1983-04-01 00:00:00

abstract：:Directly measured forces between DNA helices in ordered arrays have been reduced to simple force coefficients and mathematical expressions for the interactions between pairs of molecules. The tabulated force parameters and mathematical expressions can be applied to parallel molecules or, by transformation, to skewed m...

journal_title：Biophysical journal

authors： Podgornik R,Rau DC,Parsegian VA

更新日期：1994-04-01 00:00:00

abstract：:Phase-resolved monitoring of photoacoustic signals can provide information about the depth profile of a sample. We describe an application of this principle to determine the depth profiles of ligands and antitumor agents in mammalian cells. Measurements of the in-phase and quadrature components of the photoacoustic sp...

journal_title：Biophysical journal

authors： Narayanan K,Chandani S,Ramakrishna T,Rao CM

更新日期：1997-05-01 00:00:00

abstract：:Analysis of trajectories of dynamical biological objects, such as breeding ants or cell organelles, is essential to reveal the interactions they develop with their environments. Many previous works used a global characterization based on parameters calculated for entire trajectories. In cases where transient behavior ...

journal_title：Biophysical journal

authors： Huet S,Karatekin E,Tran VS,Fanget I,Cribier S,Henry JP

更新日期：2006-11-01 00:00:00

abstract：:It has long been recognized that protein dynamical processes occur over a wide temporal range. However, the functionality of this spectrum of events remains unclear. In this work, a generalized noise function analysis is applied to a collection of diverse protein dynamical systems. It is shown that a power law model w...

journal_title：Biophysical journal

authors： Dewey TG,Bann JG

更新日期：1992-08-01 00:00:00

abstract：:The effect of S-methylating cysteine-102 (cys-102) (SH----SSCH3) of yeast isozyme-1 (iso-1) ferricytochrome c has been studied using proton NMR spectroscopy. COSY, NOESY, and one-dimensional nuclear Overhauser effect (NOE) difference spectroscopies have all been used. The NMR spectrum of this derivative is very simila...

journal_title：Biophysical journal

authors： Busse SC,Moench SJ,Satterlee JD

更新日期：1990-07-01 00:00:00

abstract：:We showed recently that the high-salt transition of poly[d(G-C)]. poly[d(G-C)] between B-DNA and Z-DNA (at [NaCl] = 2.25 M or [MgCl(2)] = 0.7 M) can be ascribed to the lesser electrostatic free energy of the B form, due to better immersion of the phosphates in the solution. This property was incorporated in cylindrica...

journal_title：Biophysical journal

authors： Guéron M,Demaret J,Filoche M

更新日期：2000-02-01 00:00:00

abstract：:Specific proteins and lipids sequester to regions of cell membranes called rafts. Due to their high content of sphingomyelin (SM) and cholesterol, raft bilayers are thicker than nonraft bilayers and, at least at 4 degrees C, are resistant to Triton X-100 extraction. It has been postulated that rafts concentrate protei...

journal_title：Biophysical journal

authors： McIntosh TJ,Vidal A,Simon SA

更新日期：2003-09-01 00:00:00

abstract：:The ryanodine receptor (RyR) of skeletal muscle contains two functional domains: a carboxyl-terminal hydrophobic domain that forms the putative conduction pore of the calcium release channel, and a large cytoplasmic domain that corresponds to the "foot structure." To understand the contribution of the foot structure t...

journal_title：Biophysical journal

authors： Bhat MB,Zhao J,Hayek S,Freeman EC,Takeshima H,Ma J

更新日期：1997-09-01 00:00:00

abstract：:Rapid and robust methods are required to quantify the effect of hydrodynamic shear on protein conformation change. We evaluated such strategies in this work and found that the binding of the fluorescent probe 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid (bis-ANS) to hydrophobic pockets in the blood protein von ...

journal_title：Biophysical journal

authors： Themistou E,Singh I,Shang C,Balu-Iyer SV,Alexandridis P,Neelamegham S

更新日期：2009-11-04 00:00:00

abstract：:High resolution dynamics and structural information has been resolved from 2H solid-state NMR spectra of the Val-1 side-chain of the gramicidin channel in a lipid bilayer. Both powder pattern lineshapes and spectra from uniformly aligned samples of gramicidin in lipid bilayers have been analyzed to achieve a fully con...

journal_title：Biophysical journal

authors： Lee KC,Cross TA

更新日期：1994-05-01 00:00:00

abstract：:Actin polymerization is typically initiated at specific sites in a cell by membrane-bound protein complexes, and the resulting structures are involved in specialized cellular functions, such as migration, particle uptake, or mitotic division. Here we analyze the potential of the actin system to self-organize into wave...

journal_title：Biophysical journal

authors： Bretschneider T,Anderson K,Ecke M,Müller-Taubenberger A,Schroth-Diez B,Ishikawa-Ankerhold HC,Gerisch G

更新日期：2009-04-08 00:00:00

abstract：:We have characterized the fluorescence properties of 6-dodecanoyl-2-dimethylamine-naphthalene (LAURDAN) in pure interfaces formed by sphingomyelin and 10 chemically related glycosphingolipids (GSLs).1 The GSLs contain neutral and anionic carbohydrate residues in their oligosaccharide chain. These systems were studied ...

journal_title：Biophysical journal

authors： Bagatolli LA,Gratton E,Fidelio GD

更新日期：1998-07-01 00:00:00

abstract：:Verapamil and amlodipine are calcium ion influx inhibitors of wide clinical use. They are partially charged at neutral pH and exhibit amphiphilic properties. The noncharged species can easily cross the lipid membrane. We have measured with solid-state NMR the structural changes induced by verapamil upon incorporation ...

journal_title：Biophysical journal

authors： Meier M,Blatter XL,Seelig A,Seelig J

更新日期：2006-10-15 00:00:00

abstract：:We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the ne...

journal_title：Biophysical journal

authors： Wax A,Yang C,Backman V,Badizadegan K,Boone CW,Dasari RR,Feld MS

更新日期：2002-04-01 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:The temperature and voltage dependence of gating and conductance of sarcoplasmic reticulum K+ channels (S-R K+) isolated from adult canine hearts were studied using the reconstituted bilayer technique. Fusion of vesicles from this preparation frequently resulted in the incorporation of a single channel. Only bilayers ...

journal_title：Biophysical journal

authors： Shen WK,Rasmusson RL,Liu QY,Crews AL,Strauss HC

更新日期：1993-08-01 00:00:00

abstract：:Oriented solid-state NMR in combination with multiple-residue-specific (15)N labeling and extensive numerical spectral analysis is proposed to determine helix conformations of large membrane proteins in native membranes. The method is demonstrated on uniaxially oriented samples of (15)N-methionine, -valine, and -glyci...

journal_title：Biophysical journal

authors： Vosegaard T,Kamihira-Ishijima M,Watts A,Nielsen NC

更新日期：2008-01-01 00:00:00

abstract：:We demonstrate the ability of pulsed dipolar electron spin resonance (ESR) spectroscopy (PDS) to report on the conformation of Cu-Zn superoxide dismutase (SOD1) through the sensitive measurement of dipolar interactions between inherent Cu(2+) ions. Although the extent and the anisotropy of the Cu ESR spectrum provides...

journal_title：Biophysical journal

authors： Merz GE,Borbat PP,Pratt AJ,Getzoff ED,Freed JH,Crane BR

更新日期：2014-10-07 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and phospholamban (PLB) are essential for intracellular Ca2+ transport in myocytes. Ca2+-dependent activation of SERCA-PLB provides a control function that regulates cytosolic and SR Ca2+ levels. Although experimental and computational studies alone have led to a greater...

journal_title：Biophysical journal

authors： Raguimova ON,Aguayo-Ortiz R,Robia SL,Espinoza-Fonseca LM

更新日期：2020-11-03 00:00:00

abstract：:The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular co...

journal_title：Biophysical journal

authors： Tursun P,Tashiro M,Konishi M

更新日期：2005-03-01 00:00:00

abstract：:Fluorescence cross-correlation spectroscopy (FCCS) is used to determine interactions and dissociation constants (K(d)s) of biomolecules. The determination of a K(d) depends on the accurate measurement of the auto- and cross-correlation function (ACF and CCF) amplitudes. In the case of complete binding, the ratio of th...

journal_title：Biophysical journal

authors： Foo YH,Naredi-Rainer N,Lamb DC,Ahmed S,Wohland T

更新日期：2012-03-07 00:00:00