Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation.

abstract：:The 77-K resonance Raman vibrational spectrum of intact goldfish rod photoreceptors containing 3,4-dehydro (A2) retinal is dominated by scattering from the 9-cis component of the steady state at all excitation wavelengths. Intact goldfish photoreceptors were regenerated with an A1-retinal chromophore to determine whet...

journal_title：Biophysical journal

authors： Barry B,Mathies RA,Pardoen JA,Lugtenburg J

更新日期：1987-10-01 00:00:00

abstract：:Label-free imaging techniques such as differential interference contrast (DIC) allow the observation of cells and large subcellular structures in their native, unperturbed states with minimal exposure to light. The development of robust computational image-analysis routines is vital to quantitative label-free imaging....

journal_title：Biophysical journal

authors： Chaphalkar AR,Jawale YK,Khatri D,Athale CA

更新日期：2021-01-11 00:00:00

abstract：:Computer-assisted motion analysis coupled to flash photolysis of caged chemoeffectors provides a means for time-resolved analysis of bacterial chemotaxis. Escherichia coli taxis toward the amino acid attractant L-aspartate is mediated by the Tar receptor. The physiology of this response, as well as Tar structure and b...

journal_title：Biophysical journal

authors： Jasuja R,Keyoung J,Reid GP,Trentham DR,Khan S

更新日期：1999-03-01 00:00:00

abstract：:It is well known that electromagnetic fields cause mechanical forces. If one applies an electrical field to a suspension of microscopic particles, these particles realign themselves along the direction of the field and form pearl-chain-like aggregates. These chains are mostly single stranded but they are frequently mu...

journal_title：Biophysical journal

authors： Takashima S,Schwan HP

更新日期：1985-04-01 00:00:00

abstract：:In the last decades, it has been demonstrated that many animal species orient in the Earth magnetic field. One of the best-studied examples is the use of the geomagnetic field by migratory birds for orientation and navigation. However, the biophysical mechanism underlying animal magnetoreception is still not understoo...

journal_title：Biophysical journal

authors： Solov'yov IA,Schulten K

更新日期：2009-06-17 00:00:00

abstract：:Cardiomyocytes have intracellular diffusion restrictions, which spatially compartmentalize ADP and ATP. However, the models that predict diffusion restrictions have used data sets generated in rat heart permeabilized fibers, where diffusion distances may be heterogeneous. This is avoided by using isolated, permeabiliz...

journal_title：Biophysical journal

authors： Sepp M,Vendelin M,Vija H,Birkedal R

更新日期：2010-06-16 00:00:00

abstract：:Universal features of the peptide aggregation process suggest a common mechanism, with a first-order phase transition in aqueous solutions of the peptides being the driving force. Small system sizes strongly affect the stability of the minor phase in the two-phase region. We show manifestations of this effect in aqueo...

journal_title：Biophysical journal

authors： Singh G,Brovchenko I,Oleinikova A,Winter R

更新日期：2008-10-01 00:00:00

abstract：:A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...

journal_title：Biophysical journal

authors： Tai K,Shen T,Börjesson U,Philippopoulos M,McCammon JA

更新日期：2001-08-01 00:00:00

abstract：:Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as ...

journal_title：Biophysical journal

authors： Atanasov V,Knorr N,Duran RS,Ingebrandt S,Offenhäusser A,Knoll W,Köper I

更新日期：2005-09-01 00:00:00

abstract：:The time dependence of magnetic field effects on light absorption by triplet-state and radical ions in quinone-depleted reaction centers of Rhodopseudomonas sphaeroides strain R-26 has been investigated. Measurements on the time scale of the hyperfine interaction in the radical pair [(BChl)2+. ...BPh-.)] provided kine...

journal_title：Biophysical journal

authors： Ogrodnik A,Krüger HW,Orthuber H,Haberkorn R,Michel-Beyerle ME,Scheer H

更新日期：1982-07-01 00:00:00

abstract：:In cardiac muscle, troponin (Tn) and tropomyosin inhibit actin and myosin interactions through the steric blocking of myosin binding to F-actin. Ca2+ binding to Tn C modulates this inhibition. Thin filaments become activated upon Ca2+ binding, which enables strong binding of myosin with a concomitant release of ATP hy...

journal_title：Biophysical journal

authors： Solís C,Kim GH,Moutsoglou ME,Robinson JM

更新日期：2018-11-06 00:00:00

abstract：:Root mean-square deviation (RMSD) after roto-translational least-squares fitting is a measure of global structural similarity of macromolecules used commonly. On the other hand, experimental x-ray B-factors are used frequently to study local structural heterogeneity and dynamics in macromolecules by providing direct i...

journal_title：Biophysical journal

authors： Kuzmanic A,Zagrovic B

更新日期：2010-03-03 00:00:00

abstract：:We perform single-molecule flow experiments using confocal microscopy and a microfluidic device for shear rates up to 20,000 s-1 and present results for the shear-induced unraveling and elongation of tethered von Willebrand factor (VWF) multimers. Further, we employ companion Brownian dynamics simulations to help expl...

journal_title：Biophysical journal

authors： Wang Y,Morabito M,Zhang XF,Webb E 3rd,Oztekin A,Cheng X

更新日期：2019-06-04 00:00:00

abstract：:Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models are constructed computationally by building specific regions of the amino acid sequence as alpha helices and then positio...

journal_title：Biophysical journal

authors： Trewhella J,Anderson S,Fox R,Gogol E,Khan S,Engelman D,Zaccai G

更新日期：1983-06-01 00:00:00

abstract：:Microstructured surfaces provide a unique framework to probe cell migration and cytoskeletal dynamics in a standardized manner. Here, we report on the steady-state occupancy probability of cells in asymmetric two-state microstructures that consist of two fibronectin-coated adhesion sites connected by a thin guidance c...

journal_title：Biophysical journal

authors： Fink A,Brückner DB,Schreiber C,Röttgermann PJF,Broedersz CP,Rädler JO

更新日期：2020-02-04 00:00:00

abstract：:The products of MgATP hydrolysis bind to the nucleotide site of myosin and thus may be expected to inhibit the contraction of muscle fibers. We measured the effects of phosphate and MgADP on the isometric tensions and isotonic contraction velocities of glycerinated rabbit psoas muscle at 10 degrees C. Addition of phos...

journal_title：Biophysical journal

authors： Cooke R,Pate E

更新日期：1985-11-01 00:00:00

abstract：:The shapes of fluorescence induction curves in spinach chloroplasts, measured using double-flash pump-probe techniques, are shown to depend on the duration of the actinic flashes. For flash durations tau(0)

journal_title：Biophysical journal

authors： Valkunas L,Geacintov NE,France L,Breton J

更新日期：1991-02-01 00:00:00

abstract：:The structure of a membrane-embedded alpha-helical reference protein, the M13 major coat protein, is characterized under different conditions of hydrophobic mismatch using fluorescence resonance energy transfer in combination with high-throughput mutagenesis. We show that the structure is similar in both thin (14:1) a...

journal_title：Biophysical journal

authors： Vos WL,Schor M,Nazarov PV,Koehorst RB,Spruijt RB,Hemminga MA

更新日期：2007-11-15 00:00:00

abstract：:A general analytical description of the equilibrium and reaction kinetics of DNA multiplex hybridization has been developed. In this approach, multiplex hybridization is considered to be a competitive multichannel reaction process: a system wherein many species can react both specifically and nonspecifically with one ...

journal_title：Biophysical journal

authors： Horne MT,Fish DJ,Benight AS

更新日期：2006-12-01 00:00:00

abstract：:We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The in...

journal_title：Biophysical journal

authors： Friedrich M,Gan Q,Ermolayev V,Harms GS

更新日期：2011-04-20 00:00:00

abstract：:A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

journal_title：Biophysical journal

authors： Schwartz TL,Kado RT

更新日期：1977-06-01 00:00:00

abstract：:Protein conformational fluctuations modulate the catalytic powers of enzymes. The frequency of conformational fluctuations may modulate the catalytic rate at individual reaction steps. In this study, we modulated the rotary fluctuation frequency of F1-ATPase (F1) by attaching probes with different viscous drag coeffic...

journal_title：Biophysical journal

authors： Watanabe R,Hayashi K,Ueno H,Noji H

更新日期：2013-11-19 00:00:00

abstract：:Voltage-sensitive fluorescent dyes are commonly used to measure cardiac electrical activity. Recent studies indicate, however, that optical action potentials (OAPs) recorded from the myocardial surface originate from a widely distributed volume beneath the surface and may contain useful information regarding intramura...

journal_title：Biophysical journal

authors： Hyatt CJ,Mironov SF,Wellner M,Berenfeld O,Popp AK,Weitz DA,Jalife J,Pertsov AM

更新日期：2003-10-01 00:00:00

abstract：:Molecular dynamics simulations have been applied to the DNA octamer d(GCGCA-GAAC). d(GTTCGCGC), which has an adenine bulge at the center to determine the pathway for interconversion between the stacked and extended forms. These forms are known to be important in the molecular recognition of bulges. From a total of ~35...

journal_title：Biophysical journal

authors： Feig M,Zacharias M,Pettitt BM

更新日期：2001-07-01 00:00:00

abstract：:Time-resolved dielectric loss (TRDL) measurements are reported for the photochemical excitation of bacteriorhodopsin (bR) in solid films of Halobacterium halobium purple membranes. These measurements provide an independent confirmation for the existence of an important component of charge separation in these membranes...

journal_title：Biophysical journal

authors： McIntosh AR,Boucher F

更新日期：1991-07-01 00:00:00

abstract：:Frog skeletal muscle fibers, mechanically skinned under water-saturated silicone oil, swell upon transfer to aqueous relaxing medium (60 mM KCl; 3 mM MgCl(2); 3 mM ATP; 4 mM EGTA; 20 mM Tris maleate; pH = 7.0; ionic strength 0.15 M). Their cross-sectional areas, estimated with an elliptical approximation, increase 2.3...

journal_title：Biophysical journal

authors： Godt RE,Maughan DW

更新日期：1977-08-01 00:00:00

abstract：:Formation of the inverted hexagonal (H(II)) phase from the lamellar (L(alpha)) phase of bovine brain-extracted phosphatidylcholine (BBPC) and phosphatidylethanolamine (BBPE) was investigated using 31P-NMR with or without cholesterol. When the ratio of BBPC to BBPE was 1:1, the H(II) formation was observed in the prese...

journal_title：Biophysical journal

authors： Hayakawa E,Naganuma M,Mukasa K,Shimozawa T,Araiso T

更新日期：1998-02-01 00:00:00

abstract：:Formation of protrusions and protein segregation on the membrane is of a great importance for the functioning of the living cell. This is most evident in recent experiments that show the effects of the mechanical properties of the surrounding substrate on cell morphology. We propose a mechanism for the formation of me...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2007-12-01 00:00:00

abstract：:The characteristic equatorial X-ray pattern from a relaxed vertebrate skeletal muscle changes when the muscle is activated. In particular, there is a simultaneous decrease in the intensity of the first reflection (I10) and increase in the intensity of the second (I11). This observed change is almost reciprocal. When c...

journal_title：Biophysical journal

authors： Lymn RW

更新日期：1978-01-01 00:00:00

abstract：:Atomic force microscopy (AFM) has become an important tool for quantifying mechanical properties of biological materials ranging from single molecules to cells and tissues. Current AFM techniques for measuring elastic and viscoelastic properties of whole cells are based on indentation of cells firmly adhered to a subs...

journal_title：Biophysical journal

authors： Rosenbluth MJ,Lam WA,Fletcher DA

更新日期：2006-04-15 00:00:00