Ca2+ and Myosin Cycle States Work as Allosteric Effectors of Troponin Activation.

abstract：:A significant amount of work has been devoted to obtaining a detailed atomistic knowledge of the human hemoglobin mechanism. Despite this impressive research, to date, the ligand diffusion processes remain unclear and controversial. Using recently developed computational techniques, PELE, we are capable of addressing ...

journal_title：Biophysical journal

authors： Lucas MF,Guallar V

更新日期：2012-02-22 00:00:00

abstract：:The effects of the Ca(2+)-induced Ca2+ release blocker procaine on individual sarcoplasmic reticulum Ca2+ release channels have been examined in planar lipid bilayers. Procaine did not reduce the single channel conductance nor appreciably shorten the mean open times of the channel; rather, it increased the longest clo...

journal_title：Biophysical journal

authors： Zahradníková A,Palade P

更新日期：1993-04-01 00:00:00

abstract：:Connectin (titin) is a large filamentous protein (single peptide) with a molecular mass of approximately 3 MDa, contour length approximately 900 nm, and diameter approximately 4 nm, and resides in striated muscle. Connectin links the thick filaments to the Z-lines in a sarcomere and produces a passive elastic force wh...

journal_title：Biophysical journal

authors： Higuchi H,Nakauchi Y,Maruyama K,Fujime S

更新日期：1993-11-01 00:00:00

abstract：:The extracellular matrix contains components with remarkable mechanical properties, including fibronectin (Fn) fibers with extensibilities of >700% strain. We utilized what we consider a novel technique to quantify the extent of molecular unfolding that contributes to Fn fiber extension, and we compared this behavior ...

journal_title：Biophysical journal

authors： Bradshaw MJ,Smith ML

更新日期：2011-10-05 00:00:00

abstract：:The polyunsaturated fatty acid docosahexaenoic acid (DHA) makes up approximately 50% of the lipid chains in the retinal rod outer segment disk membranes and a large fraction of the lipid chains in the membranes of neuronal tissues. There is an extensive literature concerned with the dietary requirements for essential ...

journal_title：Biophysical journal

authors： Everts S,Davis JH

更新日期：2000-08-01 00:00:00

abstract：:beta-Lactamase, which catalyzes beta-lactam antibiotics, is prototypical of large alpha/beta proteins with a scaffolding formed by strong noncovalent interactions. Experimentally, the enzyme is well characterized, and intermediates that are slightly less compact and having nearly the same content of secondary structur...

journal_title：Biophysical journal

authors： Vijayakumar S,Vishveshwara S,Ravishanker G,Beveridge DL

更新日期：1993-12-01 00:00:00

abstract：:Measurements were made of exchange rates of oxygen, carbon dioxide, and krypton-85 with blood at 37.5 degrees C. Gas transfer took place across a 1 mil silicone rubber membrane. The blood was in a rotating disk boundary layer flow, and the controlling resistance to transfer was the concentration boundary layer. Measur...

journal_title：Biophysical journal

authors： Spaeth EE,Friedlander SK

更新日期：1967-11-01 00:00:00

abstract：:Total internal reflection (TIR) microfluorimetry was established as a method to measure continuously the volume of adherent cells and applied to measure membrane permeabilities in cells transfected with water channel homologs. Cytosol was labeled with the membrane-impermeant fluorophore calcein. Fluorescence was excit...

journal_title：Biophysical journal

authors： Farinas J,Simanek V,Verkman AS

更新日期：1995-04-01 00:00:00

abstract：:Yeast cytosine deaminase, a zinc metalloenzyme, catalyzes the deamination of cytosine to uracil. Experimental and computational evidence indicates that the rate-limiting step is product release, instead of the chemical reaction step. In this work, we use molecular dynamics to suggest ligand exit paths. Simulation at 3...

journal_title：Biophysical journal

authors： Yao L,Yan H,Cukier RI

更新日期：2007-04-01 00:00:00

abstract：:We present a theoretical model for aqueous solutions of double-stranded (ds) DNA with explicit consideration of electrostatic interactions, excluded-volume effects, van der Waals attractions, and salt ions. With reasonable parameters estimated from the DNA structure and experimental data for electrolytes, we are able ...

journal_title：Biophysical journal

authors： Li Z,Wu J,Wang ZG

更新日期：2008-02-01 00:00:00

abstract：:Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Amon...

journal_title：Biophysical journal

authors： Capitanio M,Pavone FS

更新日期：2013-09-17 00:00:00

abstract：:Myo1b is a myosin that is exquisitely sensitive to tension. Its actin-attachment lifetime increases > 50-fold when its working stroke is opposed by 1 pN of force. The long attachment lifetime of myo1b under load raises the question: how are actin attachments that last >50 s in the presence of force regulated? Like mos...

journal_title：Biophysical journal

authors： Lewis JH,Greenberg MJ,Laakso JM,Shuman H,Ostap EM

更新日期：2012-06-20 00:00:00

abstract：:Actin is one of the main components of the architecture of cells. Actin filaments form different polymer networks with versatile mechanical properties that depend on their spatial organization and the presence of cross-linkers. Here, we investigate the mechanical properties of actin bundles in the absence of cross-lin...

journal_title：Biophysical journal

authors： Rückerl F,Lenz M,Betz T,Manzi J,Martiel JL,Safouane M,Paterski-Boujemaa R,Blanchoin L,Sykes C

更新日期：2017-09-05 00:00:00

abstract：:The conformation of mononucleosome complexes reconstituted with recombinant core histones on a 614-basepair-long DNA fragment containing the Xenopus borealis 5S rRNA nucleosome positioning sequence was studied by scanning/atomic force microscopy in the absence or presence of linker histone H1. Imaging without prior fi...

journal_title：Biophysical journal

authors： Kepert JF,Tóth KF,Caudron M,Mücke N,Langowski J,Rippe K

更新日期：2003-12-01 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00

abstract：:Cytotoxic lymphocytes are key elements of the immune system that are primarily responsible for targeting cells infected with intracellular pathogens, or cells that have become malignantly transformed. Target cells are killed mainly via lymphocyte exocytosis of specialized lysosomes containing perforin, a pore-forming ...

journal_title：Biophysical journal

authors： Woodsworth DJ,Dunsing V,Coombs D

更新日期：2015-08-04 00:00:00

abstract：:Heme proteins react inhomogeneously with ligands at cryogenic temperatures and homogeneously at room temperature. We have identified and characterized a transition from inhomogeneous to homogeneous behavior at intermediate temperatures in the time dependence of CO binding to horse myoglobin. The turnover is attributed...

journal_title：Biophysical journal

authors： Agmon N,Doster W,Post F

更新日期：1994-05-01 00:00:00

abstract：:Cardiac contraction and relaxation dynamics result from a set of simultaneously interacting Ca(2+) regulatory mechanisms. In this study, cardiocyte Ca(2+) dynamics were modeled using a set of six differential equations that were based on theories, equations, and parameters described in previous studies. Among the uniq...

journal_title：Biophysical journal

authors： Snyder SM,Palmer BM,Moore RL

更新日期：2000-07-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bu...

journal_title：Biophysical journal

authors： Kaufman EN,Jain RK

更新日期：1991-09-01 00:00:00

abstract：:The mating pathway in Saccharomyces cerevisiae is one of the best understood signal transduction pathways in eukaryotes. It transmits the mating signal from plasma membrane into the nucleus through the G-protein coupled receptor and the mitogen-activated protein kinase (MAPK) cascade. According to current understandin...

journal_title：Biophysical journal

authors： Shao D,Zheng W,Qiu W,Ouyang Q,Tang C

更新日期：2006-12-01 00:00:00

abstract：:Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments a...

journal_title：Biophysical journal

authors： Mao B,Ebrey TG,Crouch R

更新日期：1980-02-01 00:00:00

abstract：:Atherosclerosis is a disease initiated by lipoprotein aggregation and deposition in artery walls. In this study, the de novo low-density lipoprotein aggregation process was examined. Nine major intermediates were identified in two stages of the aggregation process. In the aggregation stage, low-density lipoprotein mol...

journal_title：Biophysical journal

authors： Xu S,Lin B

更新日期：2001-10-01 00:00:00

abstract：:Ca2+ release from the endoplasmic reticulum (ER) contributes to Ca2+ transients in frog sympathetic ganglion neurons. Here we use video-rate confocal fluo-4 fluorescence imaging to show that single action potentials reproducibly trigger rapidly rising Ca2+ transients at 1-3 local hot spots within the peripheral ER-ric...

journal_title：Biophysical journal

authors： Cseresnyés Z,Schneider MF

更新日期：2004-01-01 00:00:00

abstract：:We investigate the effects of two structurally similar small cyclic molecules: salicylic acid and perillic acid on a zwitterionic model lipid bilayer, and show that both molecules might have biological activity related to membrane thinning. Salicylic acid is a nonsteroidal antiinflammatory drug, some of the pharmacolo...

journal_title：Biophysical journal

authors： Khandelia H,Witzke S,Mouritsen OG

更新日期：2010-12-15 00:00:00

abstract：:Bin/Amphiphysin/Rvs-homology (BAR) domains generate and sense membrane curvature by binding the negatively charged membrane to their positively charged concave surfaces. N-BAR domains contain an N-terminal extension (helix-0) predicted to form an amphipathic helix upon membrane binding. We determined the NMR structure...

journal_title：Biophysical journal

authors： Löw C,Weininger U,Lee H,Schweimer K,Neundorf I,Beck-Sickinger AG,Pastor RW,Balbach J

更新日期：2008-11-01 00:00:00

abstract：:Cell spreading is involved in many physiological and pathological processes. The spreading behavior of a cell significantly depends on its microenvironment, but the biochemomechanical mechanisms of geometry-confined cell spreading remain unclear. A dynamic model is here established to investigate the spreading of cell...

journal_title：Biophysical journal

authors： Zhao ZL,Liu ZY,Du J,Xu GK,Feng XQ

更新日期：2017-06-06 00:00:00

abstract：:Previous studies have demonstrated a calcium-dependent interaction of calmodulin (CaM) and Fas that is regulated during Fas-induced apoptosis in several cell lines, including cholangiocarcinoma, Jurkat cells, and osteoclasts. The binding of CaM and Fas has been identified on residues 231-254 of Fas; the V254N point mu...

journal_title：Biophysical journal

authors： Suever JD,Chen Y,McDonald JM,Song Y

更新日期：2008-12-15 00:00:00

abstract：:The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe to determine the viscous properties of the aqueous lumen of the ER. GFP was targeted to the ER lumen of CHO cells by transient tr...

journal_title：Biophysical journal

authors： Dayel MJ,Hom EF,Verkman AS

更新日期：1999-05-01 00:00:00

abstract：:The suprachiasmatic nucleus governs daily variations of physiology and behavior in mammals. Within single neurons, interlocked transcriptional/translational feedback loops generate circadian rhythms on the molecular level. We present a mathematical model that reflects the essential features of the mammalian circadian ...

journal_title：Biophysical journal

authors： Becker-Weimann S,Wolf J,Herzel H,Kramer A

更新日期：2004-11-01 00:00:00

abstract：:Galactosylceramides (GalCers) containing nervonoyl (24:1(Delta15(cis))) acyl chains have the capacity to assemble into nanotubular microstructures in excess water (. Biophys. J. 69:1976-1986). To define the structural parameters that modulate nanotube formation, GalCer derivatives were synthesized that contained cis m...

journal_title：Biophysical journal

authors： Kulkarni VS,Boggs JM,Brown RE

更新日期：1999-07-01 00:00:00