Catalysis-enhancement via rotary fluctuation of F1-ATPase.

abstract：:Ion channels and transporters are membrane proteins whose functions are driven by conformational changes. Classical biophysical techniques provide insight into either the structure or the function of these proteins, but a full understanding of their behavior requires a correlation of both these aspects in time. Patch-...

journal_title：Biophysical journal

authors： Kusch J,Zifarelli G

更新日期：2014-03-18 00:00:00

abstract：:We examined the effects of brief current pulses on the pacemaker oscillations of the Purkinje fiber using the model of McAllister , Noble, and Tsien (1975. J. Physiol. [Lond.]. 251:1-57). This model was used to construct phase-response curves for brief electric stimuli to find "black holes," where rhythmic activity of...

journal_title：Biophysical journal

authors： Chay TR,Lee YS

更新日期：1984-04-01 00:00:00

abstract：:4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that have terminal -C(NH2)2+ groups. The hydration changes that accompany their binding to the minor groove of the (AATT)2 sequence have been studied using the osmotic stress technique with fluorescence spectroscopy. The affinity...

journal_title：Biophysical journal

authors： Degtyareva NN,Wallace BD,Bryant AR,Loo KM,Petty JT

更新日期：2007-02-01 00:00:00

abstract：:Ca2+ regulates ryanodine receptor's (RyR) activity through an activating and an inhibiting Ca2+-binding site located on the cytoplasmic side of the RyR channel. Their altered sensitivity plays an important role in the pathology of malignant hyperthermia and heart failure. We used lanthanide ions (Ln3+) as probes to in...

journal_title：Biophysical journal

authors： Sárközi S,Komáromi I,Jóna I,Almássy J

更新日期：2017-05-23 00:00:00

abstract：:Skeletal and heart muscle excitability is based upon the pool of available sodium channels as determined by both fast and slow inactivation. Slow inactivation in hH1 sodium channels significantly differs from slow inactivation in hSkM1. The beta(1)-subunit modulates fast inactivation in human skeletal sodium channels ...

journal_title：Biophysical journal

authors： Vilin YY,Makita N,George AL Jr,Ruben PC

更新日期：1999-09-01 00:00:00

abstract：:Cardiomyocytes have intracellular diffusion restrictions, which spatially compartmentalize ADP and ATP. However, the models that predict diffusion restrictions have used data sets generated in rat heart permeabilized fibers, where diffusion distances may be heterogeneous. This is avoided by using isolated, permeabiliz...

journal_title：Biophysical journal

authors： Sepp M,Vendelin M,Vija H,Birkedal R

更新日期：2010-06-16 00:00:00

abstract：:PSII catalyzes the oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. PSII contains an oxygen-evolving complex, which is located on the lumenal side of the PSII reaction center and which contains manganese, calcium, and chloride. Four sequential photooxidation reactions are required to gener...

journal_title：Biophysical journal

authors： De Riso A,Jenson DL,Barry BA

更新日期：2006-09-01 00:00:00

abstract：:Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport; however, its precise mechanistic role ...

journal_title：Biophysical journal

authors： Crosby KC,Postma M,Hink MA,Zeelenberg CH,Adjobo-Hermans MJ,Gadella TW

更新日期：2013-05-07 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00

abstract：:Large-conductance Ca(2+)-activated K(+) channels (BK) play a fundamental role in modulating membrane potential in many cell types. The gating of BK channels and its modulation by Ca(2+) and voltage has been the subject of intensive research over almost three decades, yielding several of the most complicated kinetic me...

journal_title：Biophysical journal

authors： Rosales RA,Varanda WA

更新日期：2009-05-20 00:00:00

abstract：:The susceptibility of the Neurospora crassa (slime) cellular membrane to electroporation and electrodestruction in an alternating electric field was further investigated. The results were analyzed according to the dynamic rheological model of the cytoplasmic membrane. Based on an analytical description of membrane sus...

journal_title：Biophysical journal

authors： Pawlowski P,Szutowicz I,Rózycki S,Zieliński J,Fikus M

更新日期：1996-02-01 00:00:00

abstract：:Starzak and Starzak (1978. IEEE Trans. Biomed. Eng. 25:201-204.) proposed that, in cyclic application of current and voltage-clamps, the fidelity of the match of the output current with the original stimulus could be used to measure the spatial uniformity of voltage in a membrane. However, they failed to find such a m...

journal_title：Biophysical journal

authors： Moore JW,Stockbridge N,Schiff SJ

更新日期：1982-12-01 00:00:00

abstract：:There is no methodology for the estimation of the dynamic features of large-molecular-weight RNAs in homogeneous physiological media. In this report, a luminescence anisotropy-based method using a long-lifetime luminescent oligonucleotide probe for the estimation of the dynamic features of large-molecular-weight RNA i...

journal_title：Biophysical journal

authors： Sakamoto T,Mahara A,Yamagata K,Iwase R,Yamaoka T,Murakami A

更新日期：2005-12-01 00:00:00

abstract：:Neuronal synapses are highly specialized structures for communication between nerve cells. Knowledge about their molecular organization and dynamics is still incomplete. The large multidomain protein Bassoon plays a major role in scaffolding and organizing the cytomatrix at the active zone of neurotransmitter release ...

journal_title：Biophysical journal

authors： Jose M,Nair DK,Altrock WD,Dresbach T,Gundelfinger ED,Zuschratter W

更新日期：2008-02-15 00:00:00

abstract：:There are several examples of membrane-associated protein domains that target curved membranes. This behavior is believed to have functional significance in a number of essential pathways, such as clathrin-mediated endocytosis, which involve dramatic membrane remodeling and require the recruitment of various cofactors...

journal_title：Biophysical journal

authors： Cui H,Lyman E,Voth GA

更新日期：2011-03-02 00:00:00

abstract：:It has been repeatedly noted that the change of conformation of the molecules that serve as the ion-selective channels for sodium and potassium conductance in the nerve membrane will be accompanied by a change in the dipole moment of the molecule. This time-dependent change of dipole moment will produce transient curr...

journal_title：Biophysical journal

authors： Strandberg MW

更新日期：1977-09-01 00:00:00

abstract：:The problem of the desorption of a lipid molecule from a lipid vesicle (donor) and its incorporation into another vesicle (acceptor) at high acceptor concentrations, which has been investigated experimentally (Jones, J. D. and Thompson, T. E., 1990. Biochemistry, 29:1593-1600), is analyzed here from a theoretical poin...

journal_title：Biophysical journal

authors： Almeida PF

更新日期：1999-04-01 00:00:00

abstract：:Choice of network topology for gene regulation has been a question of interest for a long time. How do simple and more complex topologies arise? In this work, we analyze the topology of the marRAB operon in Escherichia coli, which is associated with control of expression of genes associated with conferring resistance ...

journal_title：Biophysical journal

authors： Prajapat MK,Jain K,Saini S

更新日期：2015-10-06 00:00:00

abstract：:To study possible structural changes in weak cross-bridge attachment to actin upon activation of the thin filament, two-dimensional (2D) x-ray diffraction patterns of skinned fibers from rabbit psoas muscle were recorded at low and high calcium concentration in the presence of saturating concentrations of MgATPgammaS,...

journal_title：Biophysical journal

authors： Kraft T,Xu S,Brenner B,Yu LC

更新日期：1999-03-01 00:00:00

abstract：:The energy transfer processes between carotenoids and Chls have been studied by femtosecond transient absorption in the CP29-WT complex, which contains only two carotenoids per polypeptide located in the L1 and L2 sites, and in the CP29-E166V mutant in which only the L1 site is occupied. The comparison of these two sa...

journal_title：Biophysical journal

authors： Croce R,Müller MG,Caffarri S,Bassi R,Holzwarth AR

更新日期：2003-04-01 00:00:00

abstract：:The wormlike-chain (WLC) model is widely used to describe the energetics of DNA bending. Motivated by recent experiments, alternative, so-called subelastic chain models were proposed that predict a lower elastic energy of highly bent DNA conformations. Until now, no unambiguous verification of these models has been ob...

journal_title：Biophysical journal

authors： Schöpflin R,Brutzer H,Müller O,Seidel R,Wedemann G

更新日期：2012-07-18 00:00:00

abstract：:Nuclear transport receptors (NTRs) mediate nucleocytoplasmic transport via their affinity for unstructured proteins (polymers) in the nuclear pore complex (NPC). Here, we have modeled the effect of NTRs on polymeric structure in the nanopore confinement of the NPC central conduit. The model explicitly takes into accou...

journal_title：Biophysical journal

authors： Osmanović D,Ford IJ,Hoogenboom BW

更新日期：2013-12-17 00:00:00

abstract：:The mathematics of the binomial model for quantal neurotransmitter release is considered in general terms, to explore what information might be extractable from statistical aspects of data. For an array of N statistically independent release sites, each with a release probability p, the compound binomial always pertai...

journal_title：Biophysical journal

authors： Quastel DM

更新日期：1997-02-01 00:00:00

abstract：:Cell adhesion to extracellular matrix is mediated by receptor-ligand interactions. When a cell first contacts a surface, it spreads, exerting traction forces against the surface and forming new bonds as its contact area expands. Here, we examined the changes in shape, actin polymerization, focal adhesion formation, an...

journal_title：Biophysical journal

authors： Reinhart-King CA,Dembo M,Hammer DA

更新日期：2005-07-01 00:00:00

abstract：:Skeletal muscle contraction is a canonical example of motor-driven force generation. Despite the long history of research in this topic, a mechanistic explanation of the collective myosin force generation is lacking. We present a theoretical model of muscle contraction based on the conformational movements of individu...

journal_title：Biophysical journal

authors： Lan G,Sun SX

更新日期：2005-06-01 00:00:00

abstract：:Purple photosynthetic bacteria harvest light using pigment-protein complexes which are often arranged in pseudo-organelles called chromatophores. A model of a chromatophore from Rhodospirillum photometricum was constructed based on atomic force microscopy data. Molecular-dynamics simulations and quantum-dynamics calcu...

journal_title：Biophysical journal

authors： Chandler DE,Strümpfer J,Sener M,Scheuring S,Schulten K

更新日期：2014-06-03 00:00:00

abstract：:Control of organelle abundance is a fundamental unsolved problem in cell biology. Mechanisms for number control have been proposed in which organelle assembly is actively increased or decreased to compensate for deviations from a set-point, but such phenomena have not been experimentally verified. In this report we ex...

journal_title：Biophysical journal

authors： Marshall WF

更新日期：2007-09-01 00:00:00

abstract：:We report a method to profile the torsional spring properties of proteins as a function of the angle of rotation. The torque is applied by superparamagnetic particles and has been calibrated while taking account of the magnetization dynamics of the particles. We record and compare the torsional profiles of single Prot...

journal_title：Biophysical journal

authors： van Reenen A,Gutiérrez-Mejía F,van IJzendoorn LJ,Prins MW

更新日期：2013-03-05 00:00:00

abstract：:The molecular cascade that controls switching of the direction of rotation of Escherichia coli flagellar motors is well known, but the conformational changes that allow the rotor to switch are still unclear. The signaling molecule CheY, when phosphorylated, binds to the C-ring at the base of the rotor, raising the pro...

journal_title：Biophysical journal

authors： Hosu BG,Berg HC

更新日期：2018-02-06 00:00:00

abstract：:Diluted bull semen samples were bombarded with a 24 Mev proton beam. Dose response curves for the fraction of cells which survived the bombardment and for the average velocity of the surviving cells were measured. Target theory indicated a cross section of the sensitive volume of 2.1 x 10(-10) cm(2). Respiration measu...

journal_title：Biophysical journal

authors： Rikmenspoel R,van Herpen G

更新日期：1969-06-01 00:00:00